Vectorpro技术说明书

Promega Corporation2800 Woods Hollow Road Madison, WI 53711-5399USA Telephone 608-274-4330Toll Free 800-356-9526Fax 608-277-2516InternetPRODUCT USE LIMITATIONS, WARRANTY,DISCLAIMERPromega manufactures products for a number of intended uses. Please refer to the product label for the intended use statements for specific products.Promega products contain chemicals which may be harmful if misused. Due care should be exercised with all Promega products to prevent direct human contact.Each Promega product is shipped with documentation stating specifications and other technical information.Promega products are warranted to meet or exceed the stated specifications. Promega's sole obligation and the customer's sole remedy is limited to replace-ment of products free of charge in the event products fail to perform as warranted. Promega makes no other warranty of any kind whatsoever, and SPECIFICALLY DISCLAIMS AND EXCLUDES ALL OTHER WAR-RANTIES OF ANY KIND OR NATURE WHATSOEVER,DIRECTLY OR INDIRECTLY, EXPRESS OR IMPLIED,INCLUDING, WITHOUT LIMITATION, AS TO THESUITABILITY, PRODUCTIVITY, DURABILITY, FITNESS FOR A PARTICULAR PURPOSE OR USE, MER-CHANTABILITY, CONDITION, OR ANY OTHER MAT-TER WITH RESPECT TO PROMEGA PRODUCTS. In no event shall Promega be liable for claims for any other damages, whether direct, incidental, foresee-able, consequential, or special (including but not lim-ited to loss of use, revenue or profit), whether based upon warranty, contract, tort (including negligence) or strict liability arising in connection with the sale or the failure of Promega products to perform in accordance with the stated specifications.Part# 9PIE665Revised 2/09Part# 9PIE665Printed in USA. Revised 2/09pGL4.10[luc2] Vector :Part No.Size E665A20µgDescription: The pGL4.10[luc2] Vector (a–d)encodes the luciferase reporter gene luc2(Photinus pyralis ) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.The pGL4.10[luc2] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.Concentration: 1µg/µl.GenBank ®Accession Number: AY738222.Storage Buffer: The pGL4.10[luc2] Vector is supplied in 10mM Tris-HCl (pH 7.4), 1mM EDTA.Storage Conditions:See the product information label for storage temperature recommendations. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes. These fluctuations can greatly alter product stability. See the expiration date on the product information age Notes:1.For easy transfer from one pGL4 Vector to another, the multiple cloning region is consistent throughout the pGL4 Vector series. For easy transfer between pGL3 Vectors and pGL4 Vectors, many of the restriction enzyme sites present in the pGL3 Vectors are also present in the pGL4 Vectors.2.Concentration gradients may form in frozen products and should be dispersed upon thawing. Mix well prior to use.Quality Control AssaysNuclease Assay: Following incubation of 1µg of pGL4.10[luc2] Vector in standard restriction digest buffers at 37°C for16–24 hours, no evidence of nuclease activity is detected by agarose gel electrophoresis.Physical Purity:A 260/A 280≥1.80, A 260/A 250≥1.05 at pH 7.4.Sequence: The pGL4.10[luc2] Vector has been completely sequenced and has 100% identity with the published sequence,available at: ww w w .p r o m e g a .c o m /v e c t o r s /© 2004–2009 Promega Corporation. All Rights Reserved.GenBank is a registered trademark of the U.S.Department of Health and Human Services.Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.All specifications are subject to change without prior notice.Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products.AF9PI E665 0209E665(a)READ THIS FIRST BEFORE OPENING PRODUCTThe sale of this product and its use by the purchaser are subject to the terms of a limited use label license, the full text of whichis shipped with this product and also available at: ww w w .p r o m e g a .c o m \L U L L . That text must be read by the purchaser prior to opening this product to determine whether the purchaser agrees that all use of the product shall be in accordance with thelicense terms. If the purchaser is not willing to accept the terms of the limited use label license, Promega is willing to accept the return of the unopened product and provide the purchaser with a full refund. However, if the product is opened for any reason,then the purchaser agrees to be bound by the terms of the limited use label license.(b)Australian Pat. No. 2001 285278 and other patents pending.(c)Patent Pending.(d)The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.Features List and Map for the pGL4.10[luc2] VectorMultiple cloning region 1–70luc2reporter gene100–1752SV40 late poly(A) region1787–2008Reporter Vector primer 4 (RVprimer4) binding region 2076–2095Col E1-derived plasmid replication origin 2333Synthetic β-lactamase (Amp r ) coding region 3124–3984Synthetic poly(A) signal/transcriptional pause site 4089–4242Reporter Vector primer 3 (RVprimer3) binding region4191–4210Promega Corporation · 2800 Woods Hollow Road·Madison, WI 53711-5399 U.S.A. ·Toll Free in the USA 800-356-9526 ·Telephone 608-274-4330 ·Part# 9PIE665Printed in USA. Revised 2/094721M A9 15 18 24 26 28 34 42 47 60 665´...ACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCTGGCCTAACTGGCCGGTACCTGAGCTCGCTAGCCTCGAGGATATCAAGATCTRVprimer3SfiI BglIBglIINheI XhoI EcoRV Acc65I KpnI EcolCRI SacISfiI BglI HindIII F i g u r e 2. T h e m u l t i p l e c l o n i n g r e g i o n o f t h e p G L 4 V e c t o r s .Sequence information and restriction enzyme tables for the pGL4 Vectors are availableonline at: ww w w .p r o m e g a .c o m /v e c t o r s /Further information on the use of pGL4 Vectors is available in Technical Manual #TM259,which is available online at: ww w w .p r o m e g a .c o m /t b s /。

Using Vector NTI Advance™ 10.0¾Opening Vector NTI Advance™ 10.01. On your Windows® desktop, click on the Start button.2. Select Programs > Invitrogen > Vector NTI Advance 10 to open the menu ofVector NTI Advance™ modules.3. Select Vector NTI Explorer to open the Explorer or Vector NTI to open theMolecule Viewer,as shown in Figure 1.Molecule ViewerVector NTI Explorer Figure 1. Opening the Molecule Viewer and Vector NTI Explorer.¾Vector NTI ExplorerVector NTI Explorer is the main tool for accessing the information in your local Vector NTI Advance™ database. Using the Explorer, you can import, open, export, and organize molecules and other database items, and launch other Vector NTI Advance™ modules (Figure 2). To launch Vector NTI Explorer:• In the Molecule Viewer, click on the Local Database icon(), or• From the Windows®Start menu, select Programs > > Invitrogen > Vector NTI Advance 10 > Vector NTI Explorer.Local/SharedData ExchangeList ofdatabaserecordsFigure 2. Vector NTI Explorer WindowFigure 3. List of object types.¾ Molecule ViewerThe Molecule Viewer displays information about DNA, RNA, and protein molecules. Tolaunch the Molecule Viewer :• From the Windows® Start menu , select Programs > Invitrogen> Vector NTIAdvance 10 > Vector NTI , or• Double-click on a molecule name in the Vector NTI Explorer . To open a molecule from within the Molecule Viewer , click on the Open button() onthe main toolbar and select the molecule name from the dialog box.The molecule will be loaded into the Molecule Viewer .Pane-specific Graphics PaneText Pane (info about features, restriction site, etc.) Sequence PaneFigure 4. Molecule Viewer windowText Pane Graphics PaneFigure 5. Pane-specificFigure 6. Text PaneFigure 7. Graphics PaneFigure 8. Sequence pane¾ Create a Molecule Display WindowThere are 2 different ways of creating new DNA/RNA in Vector NTI:• Importing from GenBank/GenPept, EMBL/SWISS-PROT and FASTA formats .The sequence and Feature map are converted from the file, and the newmolecule becomes part of the Vector NTI database.1. Open the Vector NTI Explorer.2. Select theDNA/RNA Molecules(Main) within the database table combo box.3. Select the Importand click the Moleculefrom Text file…5. Click OK.4. Import molecules from GenBank/GenPept, EMBL/SWISS-PROT and FASTA formats.6. Choose the file that you want to import and click Open.10. Double click the created file to open. 11. The result is showed in Molecule Viewer. 9. Select the molecule type on DNA/RNA molecule page and click OK.•Creating new molecules from user-defined nucleotide sequences. These can be manually entered or pasted from the clipboard and the sequence entered as a new molecule.1. Open File > Create New Sequence >Using Sequence Editor (DNA/RNA)… within the Molecule Viewer.3. On Sequence and Maps , click sequence…to add the nucleotide sequences.¾ Working with a Molecule’s Graphical RepresentationVector NTI also lets you manually format graphical maps and change the predefined display style for elements of feature and restriction maps. You can also change the shape and drawing style of features, move and format text, add text annotations, etc.A. Add features within molecule1. Select Graphics Pane.2. Click Add Feature.3. Choose the Feature.4. Create the Feature Name.5. Add the Position of Feature.6. Select Complementary when you want to change the direction of the feature in the molecule.7. Click OK.Show graphical maps in the Graphics Pane.B. Change the Graphics DisplayThere are 2 different ways of Change the Graphics Display in Vector NTI:•Change the Graphics Display, click on the Graphics Display Setup ( ) on the main toolbar.1. Select the feature that you want tochange the graphics display.2. Click Graphics Display Setup.Show theFeature type8. Click OK.• The other way, click on the Edit Picture () on the main toolbar.1. Click Edit Picture.3. On the Fill page , select a color from the color menu. 2. Select the feature that you want to change the graphics display and right click to choose Properties.5. Click OK. to close the dialog box.Show the result.¾Generating Restriction MapsRestriction maps of DNA/RNA molecules can be quickly generated in Vector NTI. For unsequenced molecule regions, you may enter the known positions of restriction sites. All the molecule descendants inherit these sites.There are 2 different ways of Generating Restriction Maps in Vector NTI:•Generating Restriction Maps, select Analyses > Restriction Analyses > Restriction Site…to open the Restriction Map Setup dialog box.•Click on the Display Setup () on the main toolbar to open the MoleculeDisplay Setup dialog box.2. Select RMap Setup… to open Restriction Map Setup dialog box. 1. Click Display Setup .4. Choose the restriction enzyme and click OK.5. Click OK > OK to close the dialog box.Display the Restriction Mapwithin the molecule on theGraphic Pane.Restriction site is shownon the Sequence pane.¾Primers Design and PCR AnalysisVector NTI can design PCR primers, sequencing primers and hybridization probes and save them to the database for future use. Using parameters you have defined, Vector NTIcan analyze those primers and probes or those you have defined yourself to determine thebest ones for optimal experimental results.4. Edit the Product Length(for example, up to maximum).to open the Choose Database Enzyme dialog box.8. Click OK to close the box.9. Highlight the PCR product.11. Create the PCR product name. 12. Click OK .13. Insert the molecule into subset in the Local Database and click OK .14. Open the Local Database.15. Double click this file to openon Molcule Viewer.¾ Molecule ConstructionConstruction means creating a DNA molecule from fragments that are completely defined and made compatible by the user.1. Open the first fragment from Local Database.2. Then open the second molecule from Local Database again.Two display windows are now open.3. Select List> Show Lists to open the Listsdialog box.4. Press Add > Add Fragment > of the name of the molecule of interest (First Fragment) to open the Fragment Wizard dialog box.5. Select theConstruction Fragment option, leave the Insert Inverted option unchecked and click the Next > to proceed.8. Click the Add to List to add the first fragment to the list.10. Select the Construction Fragment option, choose the Insert Inverted option checked, and click the Next>to proceed. 9. Press Add > Add Fragment > of the name of the molecule of interest (Second Fragment) to open the Fragment Wizarddialog box.15. Click Run to create the new DNA molecules.20. Click Yes to create constructs.。

Vectorpro技术说明书Vectorpro技术说明书VECTOR PRO技术说明书北京创宇星通科技有限公司2005年3月VECTOR PRO技术说明－实现GPS定位定向VECTOR能够提供可靠的，高更新率的位置信息和指向信息是依靠以下的方式：VECTOR使用了两个高性能GPS模块和两个多路径抑制天线来处理GPS信号，其中一对GPS 模块和天线被指定为主GPS，另外一对被指定为辅GPS。

位置结算是参考主GPS天线的相位中心位置，方位信息是参考由主GPS天线相位中心和辅GPS天线相位中心组成的基线。

VECTOR外壳上方的方向箭头是用于指示解算出的指向（当VECTOR的安装方向平行于载体的首尾连线），这个箭头位于辅GPS天线的上方。

移动RTK基准站RTKVECTOR内部的GPS模块利用L1频段的C/A码和载波相位数据解算主GPS天线和辅GPS 天线的位置关系，可以达到厘米以内精度。

利用RTK技术，计算辅GPS天线位置的方法是遵从于主GPS天线位置，当主GPS天线是移动的，通常被看作移动基准站RTK。

（moving base station Real-Time Kinematic）。

在移动基准站RTK的算法当中，VECTOR 预先强制辅GPS天线是在距离主GPS天线半径为0.5米的圆内，从而提高了辅天线与主天线的相对位置精度。

辅助传感器缩短解算时间：VECTOR除了二组GPS模块外，还包含了一组陀螺、磁罗经、倾斜传感器。

他们发挥不同的作用使RTK的初始化过程更快、更可靠。

提供方位备份：在丢失GPS信号期间，VECTOR可以依靠陀螺和磁罗经提供指向数据。

安装之前您需要了解的：1．VECTOR的安装位置将直接影响接收的效果，位置和指向的精度。

应该尽量远离其他电子设备和天线（尤其是视频天线）。

值得注意的是：位置解算是依照VECTOR主GPS天线的相位中心位置，即中心线上，距离尾部5.7CM的位置。

2．选择VECTOR的安装位置时还要考虑适合接收广播信号，不要安装在凹陷的地方，尽量高于其他平面。

To Configure with an Ethernet Cable:Although the CC Vector can be set up from a tablet or a smartphone, it may be easier to use a computer using the included 18” Ethernet cable.Connect the Ethernet cable to your computer’s Ethernet port and to either of the CC Vector’s Ethernet ports on the back of the device. Configure Wirelessly (most popular way):(Internet browser required).1. From your computer, tablet, smartphone or WiFi device, go to the WiFi Settings/Connections and connect to the WiFi Network Name listed on the sticker underneath theCC Vector.2. Enter the WiFi Password when prompted.1WiFi Network Name ORNOTE:Please do not skip any steps. Steps “B1” or “B2” must be done before proceeding.121After plugging in the AC Power cord into the CC Vector Repeater, wait 1 - 2 minutes.Leave this unplugged Attach the small antenna to the CC Vector ™53Connect the 1.5 ft. USB Amplifier cable 4Plug into the CC Vector ™ Connect the CC DX Mile ™to the 30 ft.cable. Make sure to insert cable tip completely inside connector.Connect the AC power plug into a wall outlet 6Attach the large antenna:Please follow the included Antenna Assembly /Mounting guide.See Page 5 for weatherproofing.™Please assemble all items as pictured before proceeding.V15-20222After confirming you have completed Step “B”, open the Internet Browser screen on your device and enter 192.168.18.1 into the Address Bar. Press “Enter” on your keyboard.NOTE: At this point you are connected to the CC Vector but your browser will likely tell you that you are not connected to the internet.This is normal because the CC Vector is not yet setup. Continue with the setup.NOTE: For best performance, your signal should be higher than 50%. For a better signal, try adjusting the orientation and placement of the USB antenna - then click “Rescan” to see if the signal is higher.NOTE: If network in Step “D” does not require a password, then you will not get thisscreen. Proceed to Step “F”.Click onthe WiFinetwork thatyou wantto repeat..3The system will reboot. Please make sure your computer, phone or tablet stays connected to “CCrane” and does not automatically connect to another WiFi network. If no response after 1 minute then close and re-open your web browser screen.If “Status” is “Connected” and “Internet Access” is “Yes”, then you are finished with the setup.Bookmark this page with your web browser in case you need to return to it to check the connection or change networks. You can now close this screen and continue using the internet normally.You should now see a screen similar to this: (Screens may vary on some devices).If “Status” is “Connected” and “Internet Access”is “No”, please wait for 5 to 10 minutes. If no change, please see “Troubleshooting and Tips” #2.Troubleshooting and Tips1. No “Network Names” are showing under the titles for Step “D” under “Pick network to extend” or it continues to show “Scanning”.a. No WiFi networks in range of the USB WiFi Receiver and antenna. Try moving or re-adjust the USB WiFi Receiver (CC DX Mile) and antenna.b. Check to make sure that the small USB end of the cable is properly inserted fully into the USB WiFi receiver’s (CC DX Mile) connector.c. Make sure to use the main USB connector of the USB cable and that it is inserted into the USB Port of the USB Amplifier Cable. After above is checked, click Rescan. Call C. Craneat (800) 522-8863 if you are still not able to see any “Network Names” in Step “D”.4Troubleshooting and Tips - Continued2. The “Status” shows “Connected” and “Internet Access” shows “No”In some cases, if you are at a public WiFi such as an RV Park, Hotel, Hospital, coffee shop, etc. that requires a Username & password, or to accept the User’s Terms and Agreement, open your internet browser and follow their instructions.3. If your CC Vector WiFi Repeater is not working as it should, dropping connections or not being responsive, you should first do a reset of the repeater to see if the problems persist. Other scenarios where you might want to reset your repeater is if you've forgotten the password or mis-configured it and can't get access anymore.To reset your CC Vector WiFi Repeater please follow these steps:- Power up your CC Vector. - Press and hold the reset button on the bottom of the unit for 15 seconds (Use a paper clip or a non-sharp object). - Repeat Steps A1 through G.This process usually works for resetting the CC Vector WiFi Repeater to its factory setting.4. Error Messages “WiFi Connection Failed” - Most likely the password entered for the selected Network to repeat is incorrect. Re-enter correct password.“Connection lost – The Device connection lost” - Your device has lost connection with the CCRANE Network. Most likely your computer, laptop or smartphone re-connected to another nearby network instead of the CCRANE network. Please check your connection to the CCRANE network.Additional Tips• For best performance we recommend 5 to 15 feet of separation between the CC Vector and the USB Antenna.• In some cases you may receive faster WiFi by rotating the USB Antenna horizontally, making sure to aim the broad side towards the distant WiFi network.• It is normal to receive some speed reduction when repeating a WiFi network (because it has to deliver the signal twice).• Some devices such as smartphones, tablets, printers, etc. offerWPS as an alternate form of WiFi connection which does not require a password.To connect devices to the CC Vector without entering a password, press the “WPS ” button when prompted by your device to connect. or WiFi device. •Check the WPS instructions for your computer“WPS” buttonPlease visit our website at for additional information or call 800-522-8863.5WeatherproofingOnce the antenna is securely screwed onto the CC DXMile, use the supplied coaxial seal to completely coverthe connection.We also recommend coating the USB connection withsilicone grease and wrapping with the supplied coaxial seal for added protection.2Before connecting the CC DX Mile to the antenna, coatthe threads of the antenna with the supplied siliconegrease.This will help protect the threads from rust and water incursion.1Cold Weather Protection – Below 32⁰ for the CC DX MileThe CC DX Mile can become intermittent at about 32 degrees F. Hereis a simple suggestion to give it another 10 degrees of protection downto about 22 degrees. The CC DX Mile creates a small amount of heatthat can be retained by using a 10” long piece of pipe insulation madefor 1-1/2” outside diameter pipe. The insulation shown has an “outsidediameter” of about 3”. Three layers of electric tape can be used tocompress and seal the top. It is best to leave the bottom open fordrainage.We suggest removing the insulation when the weather has warmed forthe season or when it approaches 75⁰ F. The unit can be damaged orits lifetime reduced if operated at high temperatures. For temperaturesbelow 22 degrees F you could add another layer of pipe insulationmade for 3 inch pipe. One may be able to find on Amazon by carefullysearching: “Pipe Insulation Tubing 3 inch Water Resistant” andselecting the “.75 inch thickness“ size. These suggestions are forexperimentation at your own risk.21For More Speed connect a WiFi Router - (Not Required)WiFi speed is variable and depends on distance and other conditions. The CC Vector provides enough speed for browsing the Internet with several devices or streaming video on one device.To improve the WiFi speed on all your devices connect a WiFi router (not included) to the #2LAN port of the CC Vector.1) Setup the CC Vector normally using the included directions.2) Confirm that the CC Vector is working by browsing the Internet while wirelessly connected to it.3) Connect the included 18” network cable (or use your own longer network cable) to the #2 LAN port of the CC Vector.4) Connect the other end of the network cable to the “Internet” or “WAN” port on the back of your WiFi router.5) Test the setup by wirelessly connecting your computer or other device to the router’s WiFi signal and browse the Internet.6Back of CC VectorWireless RouterSpecificationsCC DX Mile Long Range General SpecificationsWireless Standard: Frequency Band: Chipset Gain:****************: ****************: ****************: *****************: Certifications: Operating Temperature: Storage Temperature:802.11 B, G, N, AC2.4 & 5 GHz23 dBm11 Mbps54 Mbps150 Mbps433 MbpsFCC, ROHS32° F - 122° F (see weatherproofing on page 5) 14° F - 149° FCC Vector WiFi Repeater General SpecificationsStandards Interface Security Power Frequency Warranty:Wired: IEEE 802.3/802.3uWireless: 802.11b, 802.11g, 802.11nWAN: 10/100BASE-TX Port * 1LAN: 10/100BASE-TX Port * 1USB 2.0 Port * 1WEP EncryptionWPA/WPA2-PSK (Pre-Shared Key)WPA/WPA2 AESWiFi Protected Setup (WPS)Input:100-240V~50/**********Output: 12V, 1A2412~2462MHz (FCC)2412~2472MHz (ETSI)2412~2484MHz (Japan)1-Year Limited7Before UsePlease read these IMPORTANT SAFETY WARNINGS before use. It is important to read and understand all instructions.Lightning You may need to ground your antenna if you live in an area prone to lightning.Power Lines Never mount an antenna near power lines. If a power line falls and comes into contact with your antenna, DO NOT TRY TO REMOVE IT. Call your local power company.Wind Installing an antenna on windy days can be dangerous. Slight winds can create strong forces against the antenna equipment. If you have any doubts about your ability to install the antenna safely, please hire a licensed, bonded professional to do the work. C. Crane is not responsible or liable for damage or injury resulting from antenna installations.1. Read and understand all safety and operating instructions prior to installing the antenna. Retain Instructions: The safety and operating instructions should be retained for future reference.2. Water and Moisture: Coaxial sealant around openings and connections of device and antenna will prevent damage when used outdoors. Use device in a vertical position.3. Never use a utility pole to mount the device and antenna or to use as a support. Never climb a utility pole.4. Antenna installations can be dangerous because many times it involves the use of a ladder and a possible fall. Do Not install antenna on a wet, snowy or windy day or if a thunderstorm is approaching especially when on top of a building or ladder.5. The antenna can be struck by lightning, which can be fatal. We recommend a licensed and insured installation by a qualified person.6. In the event of storms, disconnect the antenna from your device. This will prevent damage caused by lightning.7. The installation or dismantling of any antenna near power lines is dangerous. Each year hundreds of people are killed or injured while attempting to install or dismantle an antenna.Tips to protect your house and contents from lightning: /nlsi_lhm/lEEE_Guide.pdfCopyright ©2022 by C. Crane172 Main Street, Fortuna, CA 95540 • Phone: 1-800-522-8863 • Web: All rights reserved. No part of this booklet may be reproduced, in any form or means whatsoeverwithout permission in writing from C.Crane.。