地塞米松对中性粒细胞性哮喘模型小鼠气道炎症的影响

地塞米松对哮喘小鼠支气管肺泡灌洗液中IL-25和IFN-γ的影响陆韦;王蕾;谯明;王玉;江吉富;吴中明【摘要】Objective To investigate the mechanism of therapeutic action of dexamethasone on asthmatic mice by detecting the levels of IL-25 and IFN-γ in bronchoalveolar lavage fluid (BALF). Methods Balb/c mice with SPF grade were randomly divided into normal control group, asthma group and dexamethasone group. Asthma group and dexamethasone group were sensitized and challenged with ovalbumin ( OVA) . Dexamethasone group was intraperitoneally injected with dexamethasone one hour before challenging. The mice were executed 24 hours after the last challenge, and the HE stained pathological sections of the right lung were made. Pathological sections of lung were observed. BALF in the left lung was also collected. The total white blood cell count and absolute eosinophile ( EOS) count were observed, and the percentage of EOS was calculated. The levels of IL-25 and IFN-γwere measured with ELISA, and correlation analyses were made. Results The counts of total white blood cell and EOS, and the percentage of EOS were significantly higher in the asthma group than in the normal control group and dexamethasone group (P<0. 05). No differences were found between the normal control group and dexamethasone group. The IL-25 level was higher in the asthma group than in the normal control group and dexamethasone group (P<0. 05), and its level in the dexamethasone group was also higher than that in thenormal control group. The IFN-γlevel was lower in the asthma group thanin the normal control group and dexamethasone group (P<0. 05), while there was no significant difference between the normal control group and dexamethasone group. IL-25 was negatively correlated with IFN-γin each group. Conclusion Part of the mechanisms of dexamethasone acting on asthma are related to its inhibition on the pulmonary inflammation and promotion on the expression of IFN-γ, and possible inhibition of IL-25 expression.%目的通过检测支气管肺泡灌洗液(BALF)中白细胞介素-25(IL-25)和γ-干扰素(IFN-γ)的水平,探讨地塞米松对小鼠支气管哮喘的治疗作用机制。

地塞米松对支气管哮喘模型大鼠气道重建的影响王文建;杨莉【期刊名称】《郑州大学学报（医学版）》【年(卷),期】2007(042)001【摘要】目的:观察地塞米松对哮喘模型大鼠气道重建的影响.方法:24只SD大鼠随机分成哮喘模型组、激素干预组和正常对照组,每组8只,前2组以卵蛋白致敏并长期吸人激发大鼠慢性哮喘模型,正常对照组以生理盐水代替卵蛋白同法处理大鼠.激素干预组每次激发前给予地塞米松0.5 mg/只腹腔注射,哮喘模型组给予等量生理盐水,共4周.最后1次激发后24 h内处死大鼠,取大鼠肺组织行免疫组化测定Ⅰ、Ⅲ型胶原和转化生长因子β1(TGF-β1)的含量,同时测定气道内外径及平滑肌层、网状基底膜的厚度.结果:哮喘模型组气道壁平滑肌和基底膜层厚度及Ⅲ型胶原与TGF-β1含量均高于正常对照组和激素干预组(P均＜0.001),内外径比值低于正常对照组和激素干预组(P＜0.001),而激素干预组和正常对照组以上指标差异无统计学意义;3组间Ⅰ型胶原含量差异无统计学意义(P＞0.05).结论:地塞米松可减少气道壁胶原沉积和平滑肌增生,从而抑制哮喘大鼠气道重建,其机制可能与抑制TGF-β1的表达有关.【总页数】4页(P130-133)【作者】王文建;杨莉【作者单位】无锡儿童医院呼吸科,无锡,214002;东南大学附属中大医院儿科,南京,210009【正文语种】中文【中图分类】R9【相关文献】1.地塞米松对哮喘大鼠气道重建、肥大细胞及IL-10的影响 [J], 刘莉;余可斐;穆敬平;黎瑞红;孟忠吉;赵磊2.转化生长因子β1对不同阶段支气管哮喘模型大鼠气道平滑肌细胞外信号调节激酶通道的影响 [J], 蔺鹏翔;张焕萍3.支气管哮喘模型大鼠气道重建的特征及机制 [J], 许淑云;徐永健;张珍祥;倪望;陈士新4.寒喘舒对支气管哮喘模型小鼠气道重建的影响 [J], 洪滔;贾菠;舒坤;黄秀萍;黄云;袁可望;刘志勇;李守明5.平喘方对支气管哮喘模型小鼠气道重建的影响 [J], 朱慧华;虞坚尔;张晓峰;陈燕萍;庞惠芳;王国华;管宇;吴杰因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对哮喘小鼠气道血管生成及血管内皮生长因子表达的影响姚红卫;吕丽丽【期刊名称】《重庆医学》【年(卷),期】2012(41)36【摘要】Objective To investigate the effects of dexamethsone on airway angiogenesis and transforming vascular endothelial growth factor in chronic asthma mice. Methods The asthmatic model was established by sensitization and challenge with OVA. The mice were randomly divided into three groups ,control group,asthma group and dexamethsone treatment group. After the last chal lenge,eosinophile(EOS) counts and active VEGF level in bronchoalveolar lavage fluid(BALF) were calculated; the pathologic chan ges of bronchi and the lung tissue were evaluated; Anti Factor YUAg antibody was used to detect vessels ,The number of vessels in lamina propria and submucosa of tracheae was counted microscopically; Immuno his to chemistry were used to measure the level of VEGF in the lung tissues of asthmatic mice;the airway wallthickness(WAt/Pbm) and vascular counts were measured by using im age analysis system. Results EOS increased significantly in asthma group compared to that in Dexamethsone group,while between dexamethsone group and control group there was no significantly difference. After repeatedly OVA exposure,the density of vessels and airway wallthickness(WAt/Pbm) and the number of vascular in lung tissues were increased significantly compared with those of control group,which were also positively related with the concentrations of VEGF in lung tissue(P<0.05). Conclusion Dexam ethsone can relieve the allergic inflammation of airway, and ease off airway angiogenesis in asthmatic mice. VEGF was over ex pressed in the asthmatic mice,and may play an important role in airway angiogenesis.%目的探讨地塞米松对哮喘小鼠气道血管生成及血管内皮生长因子(VEGF)表达的影响.方法用鸡卵清蛋白(OVA)致敏和雾化激发建立慢性哮喘小鼠模型,实验分为对照组、哮喘组和地塞米松组.测定支气管肺泡灌洗液(BALF)中白细胞和嗜酸粒细胞(EOS)及活性VEGF表达的变化;行HE染色及VEGF、抗Ⅷ因子免疫组化染色,在显微镜下计数气管黏膜固有层、黏膜下层血管密度,采用医学图像分析软件测定支气管管壁厚度(WAt/Pbm)及肺组织切片中的血管数.结果哮喘组EOS 计数明显升高,气管血管密度、WAt/Pbm及肺组织切片中的血管数明显增加,与VEGF的表达呈正相关.地塞米松组与哮喘组比较炎性反应减轻,气管血管密度、WAt/Pbm及肺组织切片中的血管数减少,VEGF表达减弱,与对照组比较差异有统计学意义(P<0.05).结论 VEGF在哮喘小鼠模型气道及肺组织内过度表达,在气道血管生成过程中可能具有重要作用.早期地塞米松干预可降低哮喘模型的气道炎症,减缓气道血管生成的过程.【总页数】3页(P3854-3856)【作者】姚红卫;吕丽丽【作者单位】江苏省常州市第四人民医院呼吸内科,213001;徐州医学院附属医院老年科,江苏徐州,221008【正文语种】中文【相关文献】1.地塞米松对哮喘小鼠气道炎症反应和STAT6表达的影响 [J], 彭小华;杨远2.血管内皮生长因子受体抑制剂SU5614对哮喘模型小鼠气道炎症的影响 [J], 姜爱英;于仁志;吕玉凤;赵丽丽3.地塞米松对哮喘小鼠气道平滑肌细胞中胸腺活化调节趋化因子表达的影响 [J], 张娟;赵铭山4.地塞米松对哮喘小鼠气道平滑肌细胞增殖及转化生长因子β1表达的影响 [J], 刘平莉;吕丽丽;夏春伟;李若然;姚红卫;朱述阳5.地塞米松对哮喘小鼠中性粒细胞性气道炎症和HMGB1表达的影响 [J], 苏艳新;张明香;刘莎;王婷;谢军;邹文静;阮玲英;罗征秀;符州因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对中性粒细胞性哮喘模型小鼠气道炎症的影响摘要】目的：观察地塞米松对中性粒细胞性哮喘（neutrophilic asthma，NA）模型小鼠气道炎症的影响。

方法：C57BL雌性小鼠随机分成NA组、NA地塞米松干预（neutrophilic asthma treating with dexamethasone，NAD）组和正常（NC）组，每组8只。

NA、NAD组予卵蛋白、脂多糖致敏并卵蛋白激发；NAD组激发前腹腔注射地塞米松；NC组不做处理。

小鼠肺功能仪检测气道阻力，以阻力倍增值评价气道反应性；检测支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）有核细胞浓度及分类比例；流式细胞术检测脾脏Th17细胞及ki-67+Th17细胞比例；ELISA检测BALF IL-17浓度。

结果（1）NA组气道阻力倍增值显著高于NC组（P<0.05）。

（2）NAD组BALF有核细胞浓度、NEU%、EOS%均显著低于NA组，但仍高于NC组（均P<0.05）。

（3）NAD组脾脏Th17细胞比例显著低于NA组，但仍高于NC组（均P<0.05）；NA、NAD组脾脏ki-67+Th17细胞比例均显著高于NC组（均P<0.05），但NA、NAD组间差异无统计学意义。

（4）NAD组BALF IL-17浓度显著低于NA组，但仍高于NC组（均P<0.05）。

结论：地塞米松可能通过抑制Th17细胞及IL-17表达，减轻NA模型小鼠气道炎症；Th17细胞增殖功能增强且不受地塞米松影响可能与气道炎症持续存在有关。

【关键词】哮喘；中性粒细胞；气道炎症；地塞米松【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2015）08-0345-03Effect of dexamethasone on airway inflamation inthe mouse model of neutrophilic asthma【Abstract】Objective To observe the effect of dexamethasone on airway inflammation in the mouse model of Neutrophilic Asthma.Methods Female C57BLmice were randomly divided into NA, NAD and NC group.NA、NAD mice were sensitized by ovalbumin OVA and LPS and challenged by OVA. NAD mice were treated with dexamethasone at beginning of each challenge.NC mice withouttreatment.Airway resistance were measured and fold increase were caculated as an assessment for AHR.Total white blood cell concentration and classification of proportion were determined in the BALF.Percentages of Th17 cells and Ki-67+Th17cells in the spleen were determined by FCM.BALF IL-17 concentration were determined by ELISA. Results (1) Fold increase of airway resistance in the NA group were higher than that of the NC group (each P<0.05).（2）BALF total cell count, neutrophil and eosinophil percentages in the NAD group were lower than that of theNA group (each P<0.05), but were higher than that of the NC group(each P<0.05). (3)Th17 cell percentages in the spleen of the NAD group were lower than that of the NA group, but were higher than that of the NC group(each P<0.05). Ki-67+Th17 cells percentage in the spleen of the NA and NAD group were higher than that of the NC group(each P<0.05);Hoever,no difference were found in the NA group compared with that of the NAD group（4）BALF IL-17 concentration in the NAD group were lower than that of the NA group, but were higher than that of the NC group(each P<0.05). Conclusions Airway inflammation in the mouse model of NA were improved by dexamethasone via its inhibition on the exspression of Th17 cells.The enhanced function of Th17 cells proliferation not influenced by dexamethasone，may be responsible for the persistence of the inflammation.【Keywords】Asthma; Neutrophil;Airway inflammation; Dexamethasone哮喘是最常见的慢性呼吸道疾病，其本质是多种细胞及细胞组分共同参与的慢性气道炎症。

银杏内酯Ｂ协同地塞米松对小鼠哮喘模型气道炎症和辅助Ｔ细胞亚群影响作用的研究作者：吕进泉张晓鸣吕剑平忻悦唐燕来源：《中国现代医生》2009年第23期[摘要] 目的探讨银杏内酯B协同地塞米松对支气管哮喘气道变应性炎症及辅助T细胞亚群的作用。

方法40只BALB/c小鼠随机分成正常对照组、哮喘对照组、地塞米松组、银杏内酯B组和联合干预组,每组8只。

收集支气管肺泡灌洗液(BALF),行白细胞(WBC)及嗜酸性粒细胞( EOS)计数;应用ELISA法测定BALF中IFN-γ和IL-4水平。

结果BALF中WBC及EOS计数:3个用药组较哮喘对照组明显减少(P[关键词] 支气管哮喘; 银杏内酯B; 气道炎症; T辅助细胞亚群[中图分类号] R969 [文献标识码] A [文章编号] 1673-9701(2009)23-25-02Effects of Ginkgolide B Coordinated with Dexamethasone on Allergic Airway Inflammation and T Helper Cell Subsets in Asthmatic RatsLV Jinquan ZHANG Xiaoming LV Jianping XIN Yue TANG YanPediatrics Department,the Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China[Abstract]ObjectiveTo investigate the effects of Ginkgolide B(GB)coordinatedwith Dexamethasone(DXM)on allergic airway inflammation and T helper cell subsets in asthmatic rats. Methods Forty BALB/c rats were randomly divided into 5 groups equally:the normal group,the asthma model group,the DXM group,the GB group and the DXM+GB group. The WBC count and eosinophil(EOS)count in bronchoalveolar lavage fluid(BALF) were estimated and ELISA was used to determine the concentrations of IFN-γ and IL-4 in BALF. ResultsCompared with the asthma model group,EOS and WBC count and IL-4 level in BALF were significantly lower in the three treated groups(P[Key Words]Asthma; Gingkgolide B; Inflammation; T-lymphocyte cell subsets目前,PAF拮抗剂成为哮喘治疗的研究方向之一,本文探讨天然PAF拮抗剂——银杏内酯B 对哮喘小鼠气道炎症和T辅助细胞(Th)亚群平衡的影响。

地塞米松对哮喘小鼠模型血清CC16的影响孙惠泉;郝创利;范丽萍;葛春龙【期刊名称】《中国血液流变学杂志》【年(卷),期】2008(018)003【摘要】目的观察腹腔内注射地塞米松对小鼠支气管哮喘模型血清Clara细胞蛋白16(CC16)的影响.方法以30只健康小鼠为实验动物,随机分为3组:即正常组、哮喘组和地塞米松(DEX)组.用卵蛋白致敏、雾化建立豚鼠哮喘模型,用酶联免疫吸附试验检测血清CC16的含量并观察其在组问的含量变化.结果哮喘组小鼠血清中CC16含量明显低于正常对照组(P＜0.05);经DEX预处理后,血清CC16水平较哮喘组明显升高(P＜0.05).结论 CC16参与了哮喘的发病,可能是其中一种重要的内源性抗炎保护因子;而糖皮质激素在哮喘中的抗炎作用可能是通过提高血清CC16的浓度而实现的.【总页数】3页(P335-336,346)【作者】孙惠泉;郝创利;范丽萍;葛春龙【作者单位】苏州大学附属儿童医院,江苏苏州,215003;苏州大学附属儿童医院,江苏苏州,215003;苏州大学附属儿童医院,江苏苏州,215003;苏州大学附属儿童医院,江苏苏州,215003【正文语种】中文【中图分类】R562.25【相关文献】1.射干麻黄汤对哮喘小鼠模型气道炎症及血清IL-6、IL-1O水平的影响 [J], 隋博文;李明虎;翟平平;李明爽;王浩;王达;李成2.哮喘患者血清CC16蛋白检测的临床价值研究 [J], 张悦;王惠萱;陈忠明;杨光辉3.哮喘小鼠模型中气道重构的变化及地塞米松的干预作用 [J], 王敏;李蓓;张光环4.血清CC16含量检测在哮喘诊治中的价值分析 [J], 张悦;陈忠明;王惠萱;杨光辉5.不同控制水平哮喘患儿血清25(OH)D3、CC16水平和FeNO的变化及临床意义[J], 李远哲;郭燕军;胡文洁;施阳;侯杰;林新春;宋晓琴;丁显飞因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对哮喘小鼠气道平滑肌细胞中胸腺活化调节趋化因子表达的影响张娟;赵铭山【期刊名称】《山东医药》【年(卷),期】2012(52)5【摘要】Objective To observe the influence of expression of dexamethasone on thymus activation regulated chemo-kine(TARC) in asthma smooth muscle cells in asthmatic mice models. Methods Thirty-six mice were randomly divided into three groups: normal control group ( group A), asthma model group ( group B) , and dexamethasone treated group (group C). The asthmatic mice models were established by OVA and AL(OH)3. White cells in BALF and eosinophils (EOS) were counted, HE-stained was used to observe the changes of pulmonary histopathology. The levels of TARC and IL-4 in both serum and BALF were measured by enzyme-linked immunosorbent assay (ELISA), then the expression of TARC protein in airway smooth muscle cells was assessed with immunohistochemistry. Results The total cell numbers and EOS count of group B were significantly higher than those in group A and groupC(P<0.01), EOS counts of group C were higher than those of groupA(P<0.05). The concentrations of TARC and IL-4 in serum of group B were obviously higher than that in group A and group C(P<0.01). The concentrations of TARC and IL-4 in BALF of group B were significantlyhigher than those in group A and group C (P < 0.01); the concentrations of TARC in BALF of group C were higher than those in group A(P <0.01). The protein expressions of TARC in airway smooth muscle cells of group B were significantly higher than those in group A and group C(P <0.01). In mice serum, strong positive correlations were found between TARC and IL-4(r=0.669, P < 0.01) ; there were significant positive correlations between TARC and IL4 in BALF (r=0.845,P<0.01). Conclusions The expressions of TARC in asthma smooth musde cells of asthma mice were significantly higher than those in normal control group. Dexamethasone may inhibit TARC expression through reducing secretion of IL-4.%目的探讨胸腺活化调节趋化因子(TARC)在哮喘小鼠气道平滑肌细胞中的表达及地塞米松对TARC的影响.方法 36只小鼠随机分为对照组(A组)、哮喘模型组(B组)、地塞米松治疗组(C 组),每组12只.以卵白蛋白OVA和氢氧化铝混悬液致敏及OVA激发建立哮喘小鼠模型.行支气管肺泡灌洗液(BALF)沉渣白细胞及嗜酸性粒细胞(EOS)计数,HE染色观察肺组织病理改变,应用酶联免疫吸附实验测定血清及BALF中TARC、IL-4的浓度,并用免疫组织化学的方法测定气道平滑肌细胞中TARC蛋白的表达量.结果 B组白细胞及EOS计数明显高于A、C组(P＜0.01),C组EOS计数高于A组(P＜0.05).B组血清及BALF中TARC及IL-4的浓度显著高于A、C组(P＜0.01)；C组血清、BALF中TARC的浓度低于B组(P＜0.01).B组气道平滑肌细胞中TARC蛋白表达量较A、C组显著增高(P＜0.01).小鼠血清、BALF中TARC浓度与IL-4浓度呈正相关(r=0.669、0.845,P均＜0.01).结论 TARC在哮喘组小鼠肺组织气道平滑肌细胞中的蛋白表达量较正常组明显增多,地塞米松可能通过减少IL-4的分泌从而抑制TARC的表达.【总页数】4页(P25-28)【作者】张娟;赵铭山【作者单位】滨州医学院附属医院,山东滨州256603;滨州医学院附属医院,山东滨州256603【正文语种】中文【中图分类】R562.2【相关文献】1.卡介菌多糖核酸对哮喘小鼠胸腺活化调节趋化因子及mRNA表达的影响 [J], 郑吉善;张正霞;李昌崇;陈庆武;苏苗赏;张维溪;张海邻;董琳;陈小芳;罗运春2.MRL/Ipr小鼠肾组织胸腺和活化调节趋化因子表达检测 [J], 刘海娜;吴春玲;肖卫国3.地塞米松对哮喘小鼠气道平滑肌细胞增殖及转化生长因子β1表达的影响 [J], 刘平莉;吕丽丽;夏春伟;李若然;姚红卫;朱述阳4.地塞米松对哮喘大鼠气道平滑肌细胞增殖及ERK1/2mRNA表达水平的影响 [J], 徐妍;张焕萍5.地塞米松对变应性哮喘小鼠胸腺活化调节趋化因子及mRNA表达的影响 [J], 郑吉善;苏苗赏;李昌崇;叶乐平;董琳;罗运春;陈小芳;李孟荣;张正霞因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对哮喘大鼠Aiolos基因表达的影响摘要：目的：探讨糖皮质激素治疗哮喘的机制。

方法：SPF级wister大鼠30只，随机分成3组，每组10只。

参照Petersen LC的方法制备哮喘模型。

结果：正常对照组肺组织病理学检测未见明显气道炎症改变。

哮喘组支气管周围可见大量炎症细胞浸润，包括巨噬细胞、淋巴细胞、中性粒细胞和EOS等；地塞米松组的小气道管壁增厚，分泌物增加、肺泡壁增厚和炎症细胞浸润现象较哮喘组大鼠明显减轻。

哮喘模型组外周血白细胞总数及嗜酸粒细胞比例与正常对照组和地塞米松组比较显著升高，差异有显著性（白细胞总数与地塞米松组比较P＜0.05，嗜酸粒细胞比例与地塞米松组比较P＜0.01，二者与正常对照组比较均P＜0.01）。

正常对照组外周血白细胞与地塞米松组与地塞米松组比较无差异性P＞0.05，嗜酸粒细胞比例与地塞米松组比较差异有显著性P＜0.01。

各组大鼠淋巴细胞AiolosmRNA均有表达。

C组大鼠淋巴细胞AiolosmRNA表达量较多。

X组大鼠淋巴细胞AiolosmRNA表达量明显减少。

与C组比较有显著性差异（P ＜0.05）；D组大鼠淋巴细胞AiolosmRNA表达量与X组有不同程度的增高（P＜0.05），而比C组略低（P ＜0.05）。

结论：哮喘大鼠的Aiolos基因的表达减弱；地塞米松可增加哮喘大鼠Aiolos基因的表达；地塞米松可改善哮喘大鼠的气道炎症。

关坚词：地塞米松；支气管哮喘；Aiolo基因；模型[Abstract]Objective To explore of glucocorticoid in the treatment of asthma.[Methods] SPF level obtained wister 30 rats were randomly divided into 3 groups of 10each.[Results] Lung tissue of normal control group detected no significant changes in airway inflammation. Bronchial asthma can be seen around the large amount of inflammatory cell infiltration，including macrophages，lymphocytes，neutrophilsand EOS，etc.；inflammatory cells primarily in the bronchial submucosa and mucosaof the outer layer around small blood vessels and alveolar infiltration，etc.；see trachea stenosis，airway epithelial cell loss，significantly increased airway secretions，bronchial wall thickening. Dexamethasone group，the small airway wall thickening，increased secretions，alveolar wall thickening and inflammatory cell infiltration significantly reduced compared with asthma group. Asthmatic peripheral blood leukocytes and eosinophil percentage with the control group and dexamethasone group were significantly higher，the difference was statistically significant（total number of leukocytes and dexamethasone group P﹤ 0.05，the proportion of eosinophils and dexamethasone group P &lt；0.01，both compared with the control group were P﹤0.01）. WBC normal control group and dexamethasone group compared with the dexamethasone group，no difference P﹤0.05，the proportion of eosinophils compared with the dexamethasone group there was significant differenceP﹤0.01.Lymphocytes AiolosmRNA rats were expressed. C expression in rat lymphocytes AiolosmRNA more. X group significantly decreased the expression of lymphocyte AiolosmRNA. And C groups were significantly different（P﹤0.05）；D expression in rat lymphocytes AiolosmRNA X group with varying degrees of increased （P0.05），but slightly lower than the C group（P﹤0.05）.Conclusion1. Asthmatic rats Aiolos gene expression was decreased，2. Dexamethasone increased Aiolos gene expression in asthmatic rats，3. Dexamethasone can improve airway inflammation in asthmatic rats.Key words：dexamethasone；bronchial asthma；Aiolo gene；mod哮喘是一种常见病，我国哮喘的发病率约在1%～4%，全世界范围内哮喘发病呈逐年增加趋势[1]。

地塞米松对哮喘鼠活化T细胞核因子c1及气道炎症的影响何彩霞;张辉;肖卫兵;张小燕【期刊名称】《中国现代医学杂志》【年(卷),期】2015(25)26【摘要】目的观察地塞米松对哮喘小鼠活化T细胞核因子c1 (NFATc1)表达及气道炎症的影响.方法建立哮喘小鼠模型并收集支气管肺泡灌洗液(BALF),酶联免疫吸附法(ELISA)测定BALF中细胞因子白介素-4(IL-4)、白介素-5(IL-5)及γ干扰素(IFN-γ)水平,免疫组织化学法测定气道周围淋巴细胞中NFATc1蛋白的表达.结果哮喘小鼠IL-4、IL-5水平及NFATc1蛋白表达较正常组升高,差异有统计学意义(P ＜0.05);地塞米松干预组IL-4、IL-5水平及NFATc1蛋白表达较哮喘组降低,差异有统计学意义(P＜0.05).结论地塞米松可抑制NFATc1的表达,降低细胞因子IL-4、IL-5水平,减轻哮喘气道炎症.【总页数】4页(P37-40)【作者】何彩霞;张辉;肖卫兵;张小燕【作者单位】湖北省荆州市第三人民医院呼吸内科,湖北荆州434001;湖北省荆州市第三人民医院呼吸内科,湖北荆州434001;湖北省荆州市第三人民医院呼吸内科,湖北荆州434001;湖北省荆州市第三人民医院呼吸内科,湖北荆州434001【正文语种】中文【中图分类】R562.25【相关文献】1.地塞米松对呼吸道合胞病毒感染哮喘加重小鼠气道TSLP分泌及炎症的影响 [J], 夏虎;骆利敏;于化鹏;邓火金;蔡绍曦2.哮喘小鼠气道炎症与活化T细胞核因子c1的相关性及尼卡地平对其的干预作用[J], 何彩霞;朱述阳;卞宏;谷名晓;吴丰芹3.地塞米松对哮喘小鼠中性粒细胞性气道炎症和HMGB1表达的影响 [J], 苏艳新;张明香;刘莎;王婷;谢军;邹文静;阮玲英;罗征秀;符州4.蝉石止哮汤联合地塞米松对哮喘患者Th1/Th2细胞因子及气道炎症的影响 [J], 杨波5.蝉石止哮汤联合地塞米松对哮喘患者Th1/Th2细胞因子及气道炎症的影响 [J], 杨波因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对哮喘小鼠气道炎症反应和STAT6表达的影响彭小华;杨远【期刊名称】《东南大学学报（医学版）》【年(卷),期】2009(028)005【摘要】目的: 研究地塞米松对支气管哮喘小鼠急性气道炎症反应的作用及对信号转导子和转录激活子6(STAT6)表达的影响.方法: 用免疫组织化学法检测给予地塞米松后哮喘小鼠肺组织中STAT6的表达.结果: 哮喘组小鼠肺泡灌洗液(BALF)中细胞总数、嗜酸粒细胞(EOS)绝对值显著高于对照组(P<0.01),上述炎症细胞计数地塞米松组比哮喘组明显减少(P<0.01) .HE染色示地塞米松组小鼠气道炎症反应程度较哮喘组减轻.STAT6在气道上皮细胞表达,哮喘组气道上皮细胞中STAT6 表达较对照组增加,地塞米松组STAT6表达与哮喘组比较明显减少,较对照组增加.支气管上皮细胞STAT6蛋白与BALF中的EOS绝对值呈显著正相关.结论: 地塞米松可在一定程度上减轻哮喘小鼠的气道炎症反应,作用机制可能是通过下调肺脏组织中STAT6蛋白的过度表达,从而抑制依赖于STAT6/IL-4通路的急性气道炎症反应.【总页数】4页(P409-412)【作者】彭小华;杨远【作者单位】东南大学附属中大医院,呼吸内科,江苏,南京,210009;东南大学附属中大医院,呼吸内科,江苏,南京,210009【正文语种】中文【中图分类】R562.2;R-33【相关文献】1.STAT1、STAT6在支气管哮喘小鼠中的表达及地塞米松对其表达的影响 [J], 郑美梅;王方剑;段成城2.STAT1、STAT6在支气管哮喘小鼠中的表达及地塞米松对其表达的影响 [J], 郑美梅;王方剑;段成城3.咪喹莫特对小鼠哮喘模型气道炎症和STAT6表达的影响 [J], 金淑贤;殷凯生;卞涛;陈子庆4.地塞米松对哮喘小鼠中性粒细胞性气道炎症和HMGB1表达的影响 [J], 苏艳新;张明香;刘莎;王婷;谢军;邹文静;阮玲英;罗征秀;符州5.CpG ODN对小鼠哮喘模型气道炎症及信号转导子和转录激活子6(STAT6)表达的影响 [J], 杨远;彭小华;林勇因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对哮喘模型小鼠肺组织活性氧及线粒体基因MTCO1的影响颜鹏;邓育琼;黄杏兰;黄彩凤;赵晓庆;刘升;程喜平;刘晓东【期刊名称】《实用医学杂志》【年(卷),期】2022(38)6【摘要】目的探讨地塞米松对哮喘模型小鼠肺组织活性氧、线粒体基因MTCO1的影响。

方法动物实验采用BALB/C小鼠利用卵清蛋白诱导生成哮喘模型鼠,21只小鼠分为地塞米松组、哮喘组、空白组;所有实验小鼠检测丙二醛;使用qPCR和WB检测肺组织MTCO1。

细胞分为地塞米松组、IL-5组、空白组。

荧光检测线粒体活性氧、线粒体膜电位,使用qPCR和WB检测线粒体MTCO1。

结果哮喘组和地塞米松组肺组织中丙二醛组较空白组增高(P <0.05),地塞米松组较哮喘组进一步增高(P <0.05);qPCR示哮喘组和地塞米松组肺组织中的MTCO1较空白组降低(P <0.05);地塞米松组MTCO1较哮喘组降低(P <0.05);WB结果与qPCR具有相同的趋势。

细胞中IL-5组及地塞米松组的MTCO1对比空白组均降低(P <0.05),地塞米松组较IL5组进一步降低,差异无统计学意义(P> 0.05);对比空白组,IL-5干预组及地塞米松组的线粒体活性氧均增高,地塞米松组线粒体活性氧较IL-5组有增高的趋势;IL-5组、地塞米松组线粒体膜电位较空白组均降低,对比地塞米松组线粒体膜电位较IL-5组,有降低的趋势。

结论地塞米松降低哮喘肺组织中已异常下降的MTCO1表达并升高哮喘肺组织中已异常升高的活性氧,不利于哮喘的缓解。

糖皮质激素通过线粒体途径调控作用可以增强氧化应激反应,可能是哮喘不定期发作的潜在危险因素,抗氧化可能缓解在糖皮质激素通过线粒体途径调控造成对哮喘的不利影响。

【总页数】7页(P731-737)【作者】颜鹏;邓育琼;黄杏兰;黄彩凤;赵晓庆;刘升;程喜平;刘晓东【作者单位】广州医科大学附属第一医院;广州医科大学附属第一医院皮肤科【正文语种】中文【中图分类】R256.12【相关文献】1.地塞米松联合1,25-二羟维生素D3对慢性哮喘小鼠肺组织中NF-κB活性的影响2.含鼠白细胞介素12基因腺病毒载体对小鼠支气管哮喘模型肺组织GATA-3表达的影响3.支气管哮喘小鼠模型肺组织和肺泡灌洗液高迁移率族蛋白B1的表达及地塞米松的干预作用4.哮喘及呼吸道合胞病毒感染小鼠肺组织 NGF、LIF 表达及地塞米松对其的影响5.地塞米松对致敏性哮喘小鼠肺组织CCR3和Eotaxin表达的影响因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对致敏性哮喘小鼠肺组织CCR3和Eotaxin表达的影响金淑贤;殷凯生;卞涛【期刊名称】《中国现代医学杂志》【年(卷),期】2006(16)8【摘要】目的观察地塞米松对小鼠哮喘模型气道炎症和肺组织CC型趋化因子Eotaxin和受体CCR3表达的影响.方法建立哮喘模型,自第14天雾化吸入OVA前0.5 h,干预组分别雾化吸入咪喹莫特30 min及ip地塞米松.OVA雾化结束后24 h,每组小鼠眼球摘除采血收集血清.采用酶联免疫吸附法测定血清、支气管肺泡灌洗液及肺组织匀浆中Eotaxin水平:收集肺泡灌洗液进行细胞计数和分类;H-E染色观察肺组织炎症改变;用免疫组化方法检测肺组织Eotaxin和CCR3的表达;用Western blot方法测定肺组织CCR3表达;用逆转录-聚合酶链反应半定量检测肺组织IL-4、IFN-γ、Eotaxin、和CCR3 mRNA表达水平.结果H-E染色可见地塞米松组小鼠气道炎症程度减轻;地塞米松治疗组嗜酸性粒细胞、单核细胞和淋巴细胞比哮喘组明显减少;哮喘组小鼠血清和肺组织匀浆Eotaxin水平较正常组升高,地塞米松治疗组小鼠较哮喘组下降;Eotaxin、CCR3均在气道上皮细胞表达,哮喘组小鼠气道上皮细胞Eotaxin、CCR3较正常组增加,地塞米松治疗组较哮喘组减轻;哮喘组小鼠肺组织Eotaxin CCR3和IL-4 mRNA表达较正常组增强,地塞米松治疗组小鼠肺组织Eotaxin、CCR3和IL-4 mRNA表达较哮喘组降低.结论地塞米松减轻哮喘小鼠的气道炎症与抑制哮喘小鼠肺组织Eotaxin、CCR3蛋白和mRNA的过度表达有关.【总页数】6页(P1140-1144,1148)【作者】金淑贤;殷凯生;卞涛【作者单位】南京医科大学第一附属医院,呼吸内科,江苏,南京,210029;南京医科大学第一附属医院,呼吸内科,江苏,南京,210029;南京医科大学第一附属医院,呼吸内科,江苏,南京,210029【正文语种】中文【中图分类】R977.1;R562.25【相关文献】1.平喘颗粒对哮喘小鼠肺组织Eotaxin和CCR3表达的影响 [J], 王珏;李竹英2.百令胶囊对哮喘豚鼠骨髓及肺组织CD_(34)^+、Eotaxin及CCR3表达的影响[J], 黄艳;王小莉;刘斌;刘鑫3.mPEG5000修饰的VLA-4拮抗肽KILDVA对致敏性哮喘小鼠肺组织Eotaxin表达的影响 [J], 甄晓兰;高福禄;赵铁华4.三氧化二砷对哮喘豚鼠肺组织中CD_(34)^+祖细胞、Eotaxin及CCR3表达的影响 [J], 宋宗芳;刘鑫;黄艳;胡耀明;罗如莹5.哮喘小鼠CD34^+祖细胞和嗜酸性粒细胞的动态变化及其与CCR3/eotaxin表达的关系 [J], 李小波;张蓉映;王斌梁;杨再兴;任应鹏因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对哮喘小鼠气道重塑的影响杨远;林勇;黄静【期刊名称】《现代医学》【年(卷),期】2006(34)4【摘要】目的探讨糖皮质激素对哮喘气道重塑和肺内基质金属蛋白酶-9(MMP-9)、金属蛋白酶组织抑制剂-1(TIMP-1)表达的影响。

方法以卵蛋白致敏激发的慢性哮喘小鼠模型为对象,测定对照组、哮喘组、地塞米松组支气管基底膜周径(Pbm)、上皮黏膜层面积(WAmuc)、平滑肌面积(WAm)、气管内壁面积(WA i),并用Pbm标准化。

天狼猩红染色测定气道Ⅰ、Ⅲ型胶原的面积。

免疫组化方法测定气道MMP-9及其抑制剂TIMP-1,行免疫组化图像分析。

结果(1)地塞米松组的WAmuc/Pbm、WAm/Pbm、WA i/Pbm大于对照组而小于哮喘组,差异有显著性(P<0.05);(2)地塞米松组Ⅰ、Ⅲ型胶原面积较对照组增加而明显低于哮喘组(P<0.05);(3)哮喘组MMP-9、TIMP-1表达明显高于地塞米松组,差异有显著性(P<0.05)。

结论慢性哮喘小鼠气道壁明显增厚,而早期行地塞米松干预则可能通过调节MMP-9/TIMP-1比值来减轻气道重塑。

【总页数】4页(P219-222)【关键词】哮喘;气道重塑;地塞米松;基质金属蛋白酶-9;金属蛋白酶组织抑制剂-1;小鼠【作者】杨远;林勇;黄静【作者单位】东南大学附属中大医院呼吸科【正文语种】中文【中图分类】R562.11;Q786【相关文献】1.转化生长因子β1Ⅰ型受体拮抗剂对支气管哮喘小鼠气道炎症及气道重塑的影响[J], 李娟;沈奕;钱艳;黄茂2.地塞米松对支气管哮喘大鼠气道重塑及气道平滑肌中Toll样受体4表达的影响[J], 韦江红;莫碧文;陶赞英;黄剑伟3.NS1619干预对哮喘小鼠气道重塑及气道平滑肌肌动蛋白、血清血小板衍生生长因子-BB表达的影响 [J], 洪璨;武怡;赵中秀4.TSLP-OX40信号通路对哮喘小鼠气道重塑的影响及地塞米松的干预作用 [J], 宫静;吴根英;高修霞5.Rho/Rho激酶在哮喘小鼠气道的表达及其对气道重塑的影响 [J], 项蔷薇;朱妍艳;罗运春;王强;李昌崇因版权原因，仅展示原文概要，查看原文内容请购买。

地塞米松对急性过敏性哮喘小鼠肺AQP5表达的影响吴葆菁;朱军;檀卫平;麦贤弟;黄花荣;李静;李文益【期刊名称】《南方医科大学学报》【年(卷),期】2008(028)009【摘要】目的观察水通道蛋白5(AQP5)在急性过敏性哮喘小鼠肺组织的改变,以及地塞米松对其的影响,初步探讨AQP5在哮喘发病机制中的作用.方法建立急性过敏性哮喘小鼠模型,并随机分为急性哮喘组、对照组和地塞米松治疗组,检测支气管肺泡灌洗液(BALF)中自细胞总数及分类计数、ELISA法测定IL-5和IFN-γ水平,RT-PCR和免疫组化法检测AQP5在肺组织的表达以及观察肺组织形态学的改变.结果 (1)哮喘组BALF中白细胞总数、嗜酸性粒细胞数和IL-5水平均高于对照组(P＜0.01),IFN-γ水平低于对照组(P＜0.01),治疗后白细胞总数、嗜酸性粒细胞数和IL-5水平均明显下降(P＜0.05,P＜0.01,P＜0.01),IFN-γ水平明显上升(P＜0.01);(2)哮喘组AQP5 mRNA水平明显高于对照组(P＜0.01),治疗后表达明显下降(P＜0.01);(3)哮喘组肺组织可见较弥漫的炎性改变,黏液分泌增多,血管壁水肿明显,血管周围有大量的炎症细胞渗出物,治疗组形态学改变明显减轻:AQP5表达于正常对照组的肺泡上皮细胞、呼吸道的表层柱状上皮细胞和黏膜下腺腺泡细胞的顶膜,哮喘组表达呈强阳性,地塞米松治疗后表达明显减弱.结论 AQP5在急性哮喘小鼠肺组织内过度表达,可能与黏液高分泌有关,地塞米松可以下调AQP5的表达,可明显减轻小鼠肺部的炎性、水肿和黏液高分泌的病理生理表现.【总页数】4页(P1670-1673)【作者】吴葆菁;朱军;檀卫平;麦贤弟;黄花荣;李静;李文益【作者单位】中山大学附属第二医院,儿科,广东广州 510120;中山大学附属第二医院,药剂科,广东广州 510120;中山大学附属第二医院,儿科,广东广州 510120;中山大学附属第二医院,儿科,广东广州 510120;中山大学附属第二医院,儿科,广东广州510120;中山大学附属第二医院,儿科,广东广州 510120;中山大学附属第二医院,儿科,广东广州 510120【正文语种】中文【中图分类】R363【相关文献】1.地塞米松对急性过敏性哮喘小鼠肺水通道蛋白1的影响 [J], 吴葆菁;李文益;檀卫平;麦贤弟;黄花荣;李静2.地塞米松对125I内照射裸鼠A549肺癌细胞AQP5及存活素survivin表达的影响 [J], 张金山;邓咏梅;温戈;李园;安薇;姚红霞3.动态研究地塞米松对哮喘小鼠肺IL-25mRNA和IL-25表达的影响 [J], 陆韦;刘清亮;冉丰丰;张承旻;张承昊;苏俊4.地塞米松对肺损伤小鼠肺巨噬细胞TLR4原位表达的影响 [J], 王谦;吕晓静;周贤梅;刘会平;施斌5.地塞米松抑制NF-κBp65和HMGB1表达对小鼠重症急性胰腺炎的影响 [J], 商琼琼;曲桂武;张建民;许红梅;逢泽辉;王庆华因版权原因，仅展示原文概要，查看原文内容请购买。