DRE(M)E样本说明

Date :日期:1-8th Floor, Building #1,10th Industrial Zone, Tian Liao Community, Gong Ming Area, Guang Ming栋一至八楼Web/网址:Features特征 PLCC-2 Package. 垂直型表贴封装 Extremely wide viewing angle. 发光角度大Suitable for all SMT assembly and solder process. 适用于所有的SMT 组装和焊接工艺 Available on tape and reel. 适用于载带及卷轴 Moisture sensitivity level: 3. 防潮等级:3 Package:3000pcs/reel. 包装:3000颗/卷 RoHS compliant. RoHS 认证 Description 描述 The Orange source color devices are made with AlGaInP on Substrate Light Emitting Diode橙光LED 由AlGaInP 四种元素芯片激发而成 Applications 应用 Optical indicator. 光学指示 Indoor display. 室内显示Landscape lighting,lamp belt. 景观照明,灯带等General suitable applications.其他适合的应用 RF-201709070127Package Dimension 封装尺寸 外形尺寸:2.0*1.4*1.3mmNOTES:备注1.All dimensions units are mm. (所有尺寸标注单位为毫米)2.All dimensions tolerances are 0.2mm unless otherwise noted. (除特别标注外,所有尺寸允许公差为±0.2毫米)Electrical / Optical Characteristics at Ts=25°C 电性与光学特性 Absolute Maximum Ratings at Ts=25°C 绝对最大值 Item 项目Symbol 符号test condition 测试条件Value unit 单位 Min. Max. Typ.Forward VoltageRank B1 VfIF=20mA1.8 1.92.1V RankB2 1.9 2.0 V Rank C12.0 2.1 V Rank C2 2.1 2.2 V Rank D1 2.2 2.3 V Rank D2 2.32.4 V Dominant wavelengthRank E00wld IF=20mA600 605 602.5 nm Rank F00 605 610 nm Luminous intensityRank H20 IVIF=20mA180 230 265 mcd Rank I10230 280 mcd Rank I20 280 350 mcd Rank J10350 430 mcd Reverse Current IR VR=5V --- 10 --- uAViewing Angle 2Θ1/2 IF=20mA --- --- 120 Deg Thermal resistanceRth(j-s)IF=20mA------290 ℃/WParameter (参数) Symbol (符号)Rating (值)Units (单位)Power Dissipation (功耗) Pd 48 mW Forward Current (正向电流) IF 20 mA Peak Forward Current (峰值电流) IFP 100 mA Reverse Voltage (反向电压) VR 5 V Electrostatic Discharge(HBM)(静电) ESD 2000 V Operating Temperature (操作温度) Topr -40 ~ +85 ℃ Storage Temperature (储存温度) Tstg -40 ~ +100℃ junction temperature (结温) Tj90℃Note: 1. 1/10 Duty cycle, 0.1ms pulse width. 脉宽0.1ms,周期1/10.2. The above forward voltage measurement allowance tolerance is 0.1V. 以上所示电压测量误差0.1V.3. The above wavelength measurement allowance tolerance is ±1nm. 以上所示波长测量误差±1nm.4. the above luminous flux measurement allowance tolerance ±10%. 上述光通量的测试允许公差为±10%.5. Care is to be taken that power dissipation does not exceed the absolute maximum rating of the product. 使用功率不能超过规定的最大值.6. All measurements were made under the standardized environment of us. 所有测试都是基于我们现有的标准测试平台.7.When the LEDs are in operation the maximum current should be decided after measuring the package temperature,junction temperature should not exceed the maximum rate.LED使用的最大电流需要根据散热条件确定,结温不能超过最大值Handling Precautions 使用注意事项1>.LED operating environment and sulfur element composition cannot be over 100PPM in the LED mating usage material. This is provided for informational purposes only and is not a warranty or endorsement.LED工作环境及与LED适配的材料中，硫元素及化合物成份不可超过100PPM，单一的溴元素含量要求小于900PPM，单一氯元素含量要求小于900PPM，溴元素与氯元素总含量必须小于1500PPM（检测含量为与LED直接接触面上元素含量）。

D-二聚体标准操作规程（NycoCard D-dimer测定试剂盒）1. 检测目的：本试剂盒用于检测血浆中纤维蛋白的降解产物D-二聚体（D-dimer）的浓度。

可作为血栓性疾病诊治过程的重要辅助工具。

2.原理：基于免疫过滤试验原理，将血浆加入检测卡孔内，样品被吸入，D-dimer分子被吸附到包被D-dimer单克隆抗体的膜中，然后加入D-dimer单克隆抗体与胶体金偶联物溶液，膜中D-dimer将与抗体金偶联物发生结合反应，形成三明治嵌合体，过剩偶联物用洗涤液洗去。

样本中存在病理水平的D-dimer，检测膜显现微红色，颜色的深浅与样本中D-dimer的浓度成正比。

色泽强度可用比色卡进行对比评价，也可用读数仪（NycoCard® READER）进行读数。

3.试剂盒：NycoCard D-dimer测定试剂盒3.1试剂盒的组成：试剂盒的规格为48人份/盒3.11检测卡包装在铝箔袋内，每卡有6个检测孔，孔内的渗水膜包被D-dimer单克隆抗体。

卡已经过处理，允许使用记号笔在孔下的空白处对病人/标本的属性进行标记。

3.12偶联抗体反应液R1 1×3ml，黑盖棕色瓶，为D-dimer单克隆抗体与胶体金偶联物溶液。

3.13洗涤液R2 1×12ml，白色瓶盖，内容PH8.0的缓冲液，主要成分为小牛血清白蛋白及清洗剂。

3.14阳性质控品1×1ml，白色瓶盖，主要为纤维蛋白的降解产物与小牛血清白蛋白的缓冲液及稳定剂，参考值参见瓶签所示范围。

3.15判读比色卡1个，塑料比色卡，五个颜色逐深的孔分别代表0.3-8mg/L的5个水平D-dimer的浓度，用于判读检测结果。

3.16塑料袋2只，一只存放开启的未使用的检测卡，一只用于存放使用后的检测卡。

3.2试剂盒的储存条件：在2-8℃贮存，失效期见试剂盒上的标签。

避免储存温度超过25 ℃、光线直射和潮湿。

试剂盒室温下储存可达一周。

3.21检测卡检测卡在2-8℃保存在未开启的铝箔袋内或开启后放置在盒中的塑料袋内，可稳定至标签所示的效期。

Triage D-Dimer Test 产品说明D-Dimer 的快速定量检测T r i a g e D -D i m e r T e s t 1符号术语表请参见 q u i d e l .c o m /g l o s s a r y仅供出口。

在美国没有销售。

计用途Q u i d e l T r i a g e D -D i m e r T e s t 是一种与 Q u i d e l T r i a g e检测仪合用的荧光免疫分析方法，用来定量检测 E D T A 抗凝全血和血浆样本中交联纤维蛋白降解产物中的 D -d i m e r 含量。

该检测方法用于辅助诊断和评估疑诊为弥漫性血管内凝血或血栓栓塞疾病（包括肺栓塞）的患者。

检测概要和说明在凝血过程中，凝血酶通过蛋白水解降解掉血纤肽 A 和血纤肽 B ，将纤维蛋白原转换为可溶 性纤维蛋白。

可溶性纤维蛋白自发聚合，而 D 区则在 X I I I a 因子的催化作用下形成共价交联。

交联纤维蛋白最终通过纤维蛋白溶解途径进行降解。

纤溶酶裂解交联纤维蛋白晶格中的共价键，并释放纤维蛋白降解产物 (F D P )，其中包括由两个 D 分子片段 (D -d i m e r ) 形成的一个分子量为 200 k D a 的交联分子。

曾有学者描述，在患有包括肺栓塞 (P E ) 和深静脉血栓 (D V T ) 的静脉血栓栓塞患者中，循环 D -d i m e r 有所升高（参见 G o l d h a b e r , S .Z . (1998) N e w E n g l. J . M e d . 339; 93-104)。

检测步骤的原理Q u i d e l T r i a g eD -D i m e r T e s t 是一种一次性荧光免疫分析测试装置，用于确定E D T A 抗凝全血或血浆标本中的 D -d i m e r 浓度。

检测步骤包括向测试卡的样品端口添加几滴 E D T A 抗凝全血或血浆样本。

超低温降温仪 Revco TM RDE 系列、Forma TM FDE 系列、HERAfreeze TM HDE 系列和 Thermo Scientific TM TDE 系列安装和操作329712H61 •修订版 F • 2022 年 3 月重要请阅读本说明手册。

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目录型号 (1)安全注意事项 (2)开箱 (3)装箱单 (3)一般性建议 (4)温度监测 (4)一般用法 (4)初次装载 (4)电池舱门打开/关闭 (4)运行标准 (5)电气规格 (5)安装 (6)位置 (6)保护导体电流 (6)调平 (6)刮冰刀 (6)备份系统（可选） (7)超级绝缘机柜结构 (7)门操作 (7)压力均衡口 (7)安装远程警报连接器 (7)预期用途 (8)操作 (9)初始启动 (9)操作概述 (9)显示 (9)设置 (10)关机 (10)刮冰刀说明 (11)预期用途 (11)非预期用途 (11)注意事项和使用方法 (11)备份系统（可选） (12)CO2和 LN2注意事项 (12)安装 (12)启动 (13)操作 (13)温度记录仪（可选） (14)设置和操作 (14)更换记录纸 (14)校准调整 (14)维护 (15)清洁冷凝器 (15)清洁冷凝器过滤器 (15)垫圈维护 (15)为降温仪除霜 (15)电池维护 (15)维护计划 (16)故障排除指南 (17)错误代码 (20)保修 (21)保修（国际） (22)附录 A：警报摘要 (23)附录 B：Modbus ASCII 参数表 (25)WEEE 合规 (34)联系信息 (35)型号表 1. 适用的型号Forma - FDExxx86F*300/400/500/600A/D/VForma - FDExxx86F* - ULTS 300/400/500/600A/D/VThermo Scientific –TDExxx86F*300/400/500/600A/D/VThermo Scientific –TDExxx86F*- ULTS300/400/500/600A/D/VHERAfreeze – HDExxx86F*300/400/500/600A/D/VRevco - RDExxx86F*300/400/500/600A/D/V1|型号Thermo Fisher Scientific 超低温降温仪安全注意事项在本手册中，使用下列符号和惯例：下面列举了一些适用于本产品的重要安全防范措施：EMC（适用时）此设备的 EMC 注册仅限商业用途。

MB.1001E NF O Q U ESos modelos binoculares tienen tubos inclinados de 45 °, distancia inter-pupilar ajustable entre 48 y 75 mm y un ajuste de ± 5 dioptrías en el tubo izquierdo os modelos trinoculares tienen tubos inclinados de 30 °, la distancia inter-pupilar es ajustable entre 48 y 75 mm y un ajuste de ± 5 dioptrías en el tubo izquierdo l cabezal trinocular tiene un divisor de haz de trayectoria de luz fija (50:50)MB.1051-LCDMB.1155 MB.1153MB.1152 MB.1051I LU M I N AC I ÓN• T odas las versiones monoculares se suministran con una iluminación LED 1W de intensidad ajustable• L as versiones binoculares y trinoculares se suministran con un sistema de iluminación NeoLED ™ 1W de intensidad ajustable• T odos los modelos tienen baterías internas recargables con un cargador de batería externo de 100-240 V / adaptador de redCO N T E N I D O I N C LU I D O• S e suministra con cargador de adaptador de red, filtro blanco, cubierta anti-polvo, manual de usuario y aceite de inmersión de 5 ml para modelos con objetivo S100x. Software incluido para modelos digitales y para los modelos LCD• Todo empacado en una caja de poliestirenoMODELOS DIGI TALESC ÁM A R A• C ámara integrada CMOS USB-2 de 1.3 MP• R esolución máxima de 1272 x 952, profundidad de color de 24 bits, hasta 30 fotogramas por segundo• S e entrega con el software ImageFocus 4, cable USB-2 y un patrón de calibración de 1 mm / 100 partes • La garantía para la cámara es de dos añosS O F T WA R E• E l software ImageFocus 4 de captura y análisis permite guardar imágenes en formatos .jpg, .tif o .bmp, así como videos en formato .avi• L as imágenes se pueden anotar y las mediciones se pueden realizar en imágenes en vivo o capturadas• C ompatible con Windows 7, 8 y 10, configuraciones de 32 y 64 bits • L a versión Mac OS Light también está disponible para capturar imágenes • L as actualizaciones se pueden descargar en nuestro sitio web PA N TA L L A LC DTodos los microscopios MicroBlue LCD vienen con una pantalla LCD de 5.6”• Resolución de la pantalla 640 x 480• Las imágenes y los videos se guardan directamente en la tarjeta SD • R esolución de imagen 2048 x 1536, 1600 x 1200, 1280 x 960, 640 x 480 en formato .JPEG• Resolución video 640 x 480 en formato .AVI• Suministrado con el programa Imagebasic et cable USB.2• Garantía de 2 años para la cámaraP R O G R A M A I M AG E B A S I C• E l programa de captura permite guardar imágenes en formato.jpg y videos en formato .avi• Compatible con Windows 7, 8 et 10, en configuraciones de 32 y 64 bits • L as actualizaciones se pueden descargar desde nuestra página web EuromexMicroscopenbv•Papenkamp20•6836BDArnhem•TheNetherlands•T+31(0)263232211•F+31(0)263232833•****************•ACCESORIOS Y REPUES TOSMB.6010 Ocular de gran campo WF10x/18 mmMB.6010-P Ocular de gran campo WF10x/18 mm con puntero MB.6010-M Ocular de gran campo WF10x/18 mm micrométrico MB.6015 Ocular de gran campo WF15x/11 mm MB.6020 Ocular de gran campo WF20x/9 mm MB.6099 Pareja de protectores de gomaMB.7004 Objetivo acromático 4x/0.10, para-focal 35 mm MB.7010 Objetivo acromático 10x/0.25, para-focal 35 mm MB.7020 Objetivo acromático 20x/0.40, para-focal 35 mm MB.7040 Objetivo acromático S40x/0.65, para-focal 35 mm MB.7060 Objetivo acromático S100x/1.25, para-focal 35 mm MB.9710 Filtro esmeriladoMB.9900 Maleta de transporte de aluminioAE.9918 Bolsa de microscopio de nylon, dimensiones 26 x 18 x 400 mm MB.9975 Adaptador de red / cargador externo 100-240 Vca / 5 Vcc (50/60 Hz)MB.9981 Recambio 1W LED MB.9991 Recambio 1W NeoLEDM O D E LO SMono Bino TrinoObjetivos máximos Objetivos 4/10/S40xObjetivo S60xObjetivo S100x Platina mecánica X-YLED NeoLED BateríasMB.1001 •3•••MB.1051•4••••MB.1651•4•••••MB.1151•4•••••MB.1052•4••••MB.1652•4•••••MB.1152•4•••••MB.1053•4••••MB.1653•4•••••MB.1153•4•••••M O D E LO SDigital LCD Digital mono Objetivos máximos Objetivos 4/10/S40x Objetivo S60x Objetivo S100x Platina mecánica X-YLED NeoLED BateríasMB.1001-LCD*•3•••MB.1051-LCD*•4••••MB.1055•4••••MB.1655•4•••••MB.1155•4•••••* modelos con pantalla LCDPB.5155 P orta-objetos de 76x26 mm., cantos pulidos(caja de 50 unidades)PB.5157-W P orta-objetos de 76x26 mm., cantos pulidos. Banda blanca(caja de 50 unidades)PB.5157-B P orta-objetos de 76x26 mm., cantos pulidos. Banda azul(caja de 50 unidades)PB.5160 P orta-objetos de 76x26 mm., cantos pulidos. Con cavidad(caja de 10 unidades)PB.5165 C ubre-objetos de 18x18mm., 0.13-0.17 mm.(caja de 100 unidades)PB.5168 C ubre-objetos de 22x22mm., 0.13-0.17 mm.(caja de 100 unidades)PB.5245 Papel de limpieza de lentes (paquete de 100 hojas)PB.5255 Aceite de inmersión, n=1.482 (25 ml.)PB.5274 Alcohol isopropilico 99% (200 ml.)PB.5275 K it de limpieza compuesto por líquido de limpieza de lentes,gamuza, papel de limpieza de lentes, cepillo, pera de aire y bastoncillos de algodón.。

Common Features:•Large backlit LCD display with easy-to-read 1" digits •Type K thermometer built-in for temperature measurements •Low current capability:measure down to 0.1μA •Input fuse protection and mis-connection warnings •Complete with CAT III test leads, multi-position tilt standand velcro strap for hanging,protective holster with testlead holder, bead wiretemperature probe (-4 to 482°F/-20 to 250°C),and 9V batteryRugged design with built-in IR ThermometerExtech 400 Series Multimeters are available with or without the built-in InfraRed thermometer, select auto or manual ranging, True RMS or averaging, and choosethe meter you want at a price you can afford.Extech 400 Series MultiMetersOrdering Information EX410..............Manual Ranging MultiMeter EX410-NIST ......EX410 MultiMeter w/ NIST Certificate EX411..............True RMS Manual Ranging MultiMeter EX411-NIST ......EX411 MultiMeter w/ NIST Certificate EX420..............Autoranging MultiMeter EX420-NIST ......EX420 MultiMeter w/ NIST Certificate EX430..............True RMS Autoranging MultiMeter EX430-NIST ......EX430 MultiMeter w/ NIST Certificate EX450..............Autoranging MultiMeter + IR Thermometer EX450-NISTL ....EX450 MultiMeter w/ NIST Certificate EX470..............True RMS Autoranging MultiMeter + IR Thermometer EX470-NISTL ....EX470 MultiMeter w/ NIST CertificateAccessoriesCA500..............Case with shoulder strap and side pockets TP873..............Replacement Bead wire Temperature Probe HG500..............Magnetic Hanging StrapModel EX450•Built-in IR Thermometer•AutorangingModel EX470•Built-in IR Thermometer•True RMS•Autoranging•Capacitance•Frequency•Relative functionModel EX410/EX411•True RMS (EX411)•Manual Ranging Model EX420/EX430•True RMS (EX430)•Autoranging •Capacitance •Frequency。

D-二聚体（D-Dimer）测定试剂盒（胶体金免疫层析法）产品技术要求贝尔D-二聚体（D-Dimer）测定试剂盒（胶体金免疫层析法）适用范围：用于体外定量测定人血清或血浆中的D-二聚体(D-Dimer)含量。

1.1 包装规格20人份/盒1.2主要组成成分本试剂盒由D-Dimer检测卡、干燥剂和滴管组成。

D-Dimer检测卡由试纸条外壳与试纸条构成，试纸条由样品垫、胶体金垫（喷有由胶体金标记的D-Dimer单克隆抗体）、层析膜（T线包被有D-Dimer 单克隆抗体，C线包被有羊抗鼠IgG抗体）、吸水纸、衬垫构成。

检测卡为20人份/盒，干燥剂为1个/袋、滴管为20个/盒。

2.1 物理性状2.1.1 外观试剂盒各组分齐全、完整；包装袋应密封性好无破损；标签清晰；材料附着牢固，条宽应适应于卡壳且装配紧密。

2.1.2 膜条宽度膜条宽度应不低于4.0mm。

2.1.3 液体移行速度液体移行速度应不低于10mm/min。

2.2 空白检出限应小于0.1mg/L。

2.3 分析特异性分别用浓度为1000ng/mL肌酸激酶同工酶MB（CK-MB）、1000ng/ml肌红蛋白（Myo）、1000ng/ml心肌肌钙蛋白T（cTnT）、1000ng/ml心肌肌钙蛋白I（cTnI）、5000ng/ml C反应蛋白(CRP)分析特异性参考品进行测定，结果应小于0.1mg/L。

2.4 重复性用0.5mg/L D-二聚体(D-Dimer)参考品和2.0mg/L D-二聚体(D-Dimer)参考品重复检测10次，其变异系数（CV）应不大于15%。

2.5 准确度将D-Dimer含量为5.0mg/L人血清参考品加入到D-Dimer含量为0.1mg/L的正常人血清参考品中，参考品按照体积比1:9混合，对混合后样本进行检测，回收率应在85%～115%范围内。

2.6 线性D-二聚体(D-Dimer)检测线性范围为[0.1，5.0]mg/L，试剂盒相关系数r应≥0.99。

Products Solutions Services SD00292F/00/EN/15.1571290820Safety InstructionsSource ContainerFQG60, FQG61/62, FQG63, FQG66Radiometric level measurementSupplementary safety instructions for radioactive sources and source containers approved for use in CanadaSource Container FQG60, FQG61/62, FQG63, FQG662Endress + HauserTable of contentsIntroduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3Scope of application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Leak test procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3Equipment to be tested . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Frequency of leak testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Leak test procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3Visual check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Emergency procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . .7Objective and overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7Source Container FQG60, FQG61/62, FQG63, FQG66Endress + Hauser 3IntroductionScope of applicationThe safety instructions contained in this document apply to radioactive sources and source containers which have been approved by the CNSC for the use in Canada.This document does not substitute the Operating Instructions TI00445F (FQG60), TI00435F (FQG61/62), TI00446F (FQG63), BA01327F and Technical Information TI01171F (FQG66).When operating a source container within Canada, the respective Operating Instructions have to be strictly observed as well as the procedures described in this document.Leak test procedureEquipment to be tested A leak test is required if the source activity is greater than 50 MBq.Frequency of leak testingSubject to other regulations by CNSC the device has to be tested •once every 12 months for sources in continuous operation•once every 24 months if the source is placed into continuous storage•immediately, if the source is placed back into operation after being stored for 12 or more consecutive months (replaces any previous test that may have been done during the previous 12 months while in storage).Leak test procedure1.Proceed as follows for FQG60, FQG61/62 and FQG63:1Wiping surface for the leak test along the edge of the nameplateA FQG60; Ordering feature 240 "Emission Angle; Application", option 3 "20deg; limit switch + density"B FQG60; Ordering feature 240 "Emission Angle; Application", option 5 "40deg; level"C FQG60; Ordering feature 240 "Emission Angle; Application", option 4 "20deg; density 30deg diagonal radiation"Leak tests are required whenever an incident occurs that may damage the sealed source or shielding. The leak test must be performed as soon as possible after the incident.Ensure that the person collecting the leak test samples has:•access to and follows approved leak test procedures•received radiation safety training to control any associated radiation hazard •sufficient sampling materials and leak test sampling certificates.Source Container FQG60, FQG61/62, FQG63, FQG664Endress + HauserA FQG61/62; Ordering feature 020 "Version", option A "Cylinder lock fixation ON/OFF + covering cap"B FQG61/62; Ordering feature 020 "Version", option B "Padlock fixation OFF + locking bolt ON + rotary bracket"C FQG61/62; Ordering feature 020 "Version", option C "Padlock fixation ON/OFF + rotary bracket"D FQG61/62; Ordering feature 020 "Version",option D "Padlock fixation ON/OFF + o-ring seal double > higher protection dust/humidity + rotary bracket"EFQG61/62; Ordering feature 020 "Version",option K "Pneumatic cutoff, non Ex + padlock fixation OFF",option L "Pneumatic cutoff, ATEX + padlock fixation OFF ATEX II 2 G c IIB T4 X",option M "Pneumatic cutoff, non Ex + o-ring seal double > higher protection dust/humidity + padlock fixation OFF" or option N "Pneumatic cutoff, ATEX + o-ring seal double > higher protection dust/humidity + padlock fixation OFF +ATEX II 2 G c IIB T4 X"A FQG63, Ordering feature 020 "Version", optionB "Rotary bracket + locking bolt ON + padlock fixation OFF"B FQG63, Ordering feature 020 "Version", optionC "Padlock fixation ON/OFF + rotary bracket"2.Have the samples analysed by a CNSC approved laboratory. A source is to be considered leaking ifmore than 200 Bq is detected on a leak test sample.3.In case of an indeed leaking source:- immediately discontinue use of the device- contact the responsible radiation protection officer for instructions- take appropriate measures to control a potential spread of radioactive contamination from the source- secure the source- notify CNSC that a leaking source has been detectedSource Container FQG60, FQG61/62, FQG63, FQG66Endress + Hauser 54.In case of an indeed leaking source:- immediately discontinue use of the device- contact the responsible radiation protection officer for instructions- take appropriate measures to control a potential spread of radioactive contamination from the source- secure the source- notify CNSC that a leaking source has been detectedSource Container FQG60, FQG61/62, FQG63, FQG666Endress + Hauser1.Proceed as follows for FQG66, ordering feature 020 "Version", option L "Pneumatic cutoff +proximity switch":4.In case of an indeed leaking source:- immediately discontinue use of the device- contact the responsible radiation protection officer for instructions- take appropriate measures to control a potential spread of radioactive contamination from the source- secure the source- notify CNSC that a leaking source has been detectedVisual checkIf considerable corrosion is visible at the housing (FQG60: especially in the area of the shutter plate; FQG61/62: especially at the cover plate of the radiation channel) measure the radiation level around the device. If values occur exceeding the normal operation level, cordon off the area and contact immediately the responsible radiation safety officer for instructions. In any case corroded devices or corroded parts of devices shall be exchanged as soon as possible.For FQG63: Source containers with corroded extension element, interlocks or source holder rods require immediate exchange.For FQG66: especially at the cover plate of the radiation channel and at the welding seams of the device base.Source Container FQG60, FQG61/62, FQG63, FQG66Endress + Hauser 7Emergency procedureObjective and overviewThis emergency procedure should be put into effect immediately to secure an area in the interests of protecting personnel where an exposed source is known, or suspected, to exist.Such an emergency exists when a radioisotope is exposed either by it becoming separated from the source container or the source container shutter cannot be closed or if available, the extension element or the shutter cannot be moved to OFF position or a possible damage on fire has occurred.This procedure will safeguard an area until an appropriate radiation protection officer can attend site and advise on corrective action.The custodian of the radioactive source (the customer’s designated "authorized person") is responsible for observing this procedure.Procedure1.Determine the unsafe area by measurement (on site) or by calculation knowing the size and typeof source installed from the records.2.Cordon off the area at the boundary where the radiation level exceeds 2.5 Sv/h (0.25 mR/h) byyellow tape or rope and post international radiation warning signs.In case a fire has occurred:- Assume that some of the lead shielding has melted.- Examine the source container environment for any damage to the mounting structure (bolts, brackets, etc.).- Survey the damaged source container and compare results with initial installation survey.- In case the housing of the source container is heavily damaged, leak test the source container for removable contamination.- For FQG61, FQG62, FQG63, FQG66: Check the shutter mechanism for proper function.In case of a shutter that will not closed:FQG60:- If part of the area is accessible (e.g. a vessel in the event of a level gauge installation where there is a possibility that a person might enter), the source housing should be unbolted from itsmounting and laid face down on the ground or put emission channel towards a thick wall. The weight of approx. 18kg (39.7 lbs) allows manual handling.- Personnel should at all times be behind the source housing, not in front of the emission channel (rectangular shutter plate).FQG61/62:- If part of the area is accessible (e.g. a vessel in the event of a level gauge installation where there is a possibility that a person might enter), the source housing should be unbolted from itsmounting and laid face down on the ground or put emission channel towards a thick wall. The ring-eyelet on the housing should facilitate safe handling.- Personnel should at all times be behind the source housing, not in front of the emission channel (flange).In case of a shutter that will not close due to failure of the pneumatic drive:Switch off or disconnect compressed air, remove supply lines if necessary. Open lower padlock, unscrew the allen screws adjacent to the pneumatic drive and remove the complete drive unit to get access to the source holder. Turn source holder manually to the marked off position.FQG63:In case the extension element is retracted but the swivel insert does not close:- If part of the area is accessible (e.g. a vessel in the event of a level gauge installation where there is a possibility that a person might enter), the source housing (extension element in OFF position) should be unbolted from its mounting and laid face down on the ground or put emission channel towards a thick wall. The ring-eyelet on the housing should facilitate safe handling.- Personnel should at all times be behind the source housing, not in front of the emission channel (flange).In case the extension element is not retractable:If part of the area is accessible (e.g. a vessel in the event of a level gauge installation where there is a possibility that a person might enter), the source container should be unbolted, removed together with the inner proctection pipe from its mounting and laid down on the ground. The protection pipe should be immediately completely covered with a suitable shielding.Source Container FQG60, FQG61/62, FQG63, FQG668Endress + HauserPersonnel should at all times keep maximum possible distance from the protection pipe and should at all times be behind the source container, not in front of the emission channel (flange of the source container) or near the extension element or near the protection pipe. The eye bolts on the housing should facilitate safe handling.FQG66:- If part of the area is accessible (e.g. a vessel in the event of a level gauge installation where there is a possibility that a person might enter), the source housing should be unbolted from its mounting and> in case the radiation emission channel is located horizontally: put the emission channel towards a very thick wall, or> in case the radiation emission channel is located vertically: put the source container on its device base on the ground.Risk of injury‣ Use lifting tools due to the weight of approx. 435 kg (959.18 lb).- Personnel should at all times be behind the source housing, not in front of the emission channel (flange).1Eye bolt2Connection screw3.If it is not practical to cordon off the entire area or if the source is in immediate danger of moving,it may be necessary to secure the source by relocating it or adding shielding. Here, the inverse square law should be observed, i.e. radiation reduces with distance quadratically. The source should only be handled via pliers or tongs and held as far away from the body as possible. The time taken to fulfill the exercise should be minimized by rehearsal prior to rm the local Canadian Nuclear Safety Commission Duty Officer responsible for the area inwhich the incident has occured, and ask for immediate action.Telephone number: (613) 995-0479 (Ottawa)This is to be done as soon as possible and not later than 24 hours of the incident being discovered.5.After thorough assessment of the damage, the CNSC Inspector, in conjunction withEndress+Hauser, will agree a remedy to the specific problem.CAUTIONSource Container FQG60, FQG61/62, FQG63, FQG66Endress + Hauser9Source Container FQG60, FQG61/62, FQG63, FQG66 10Endress + HauserSource Container FQG60, FQG61/62, FQG63, FQG66Endress + Hauser1171290820。

S A M P L E R SItem Portfolio SamplerSingle Copy Sampler (Box)Description Use for a customer’s first introduction to the program.Includes:Program Guide,System 44Research Foundation Paper,SPI Assessment Paper,Product T our CD,and Print Component Sampler.Use at conferences,events and with customers who have already been introduced to the program and are serious about purchasing.Includes the Program Guide,System 44Research Foundation Paper,SPI Assessment Paper,Product T our CD,Component Map,Poster,44Book ,Decodable Digest ,T eaching Resources Guides,Screening ,Assessment and Reporting Guide and an assortment of Student Library Books.Item #159280159279Availability at JC 9/1/089/1/08Availability on the Portal N/A N/AUnitCost$12.50*$34.00*P R O M OT I O N A L M AT E R I A L SItemProgram GuideProduct T our CD Simulator CDComponent MapTeaching Guide Sampler (Book)“Tiny TE”DescriptionComprehensive overview of the program.Great introduction for customers—perfect leave behind.T otal pages:36.Content currently featured on the System 44Web site.This CD allows customers to get an overview of the program,and hear from Marilyn Adams and T ed Hasselbring.(This is a Flash®file—NO Internetconnection needed.)T abs have been added for easier navigation.Use this CD to better familiarize yourself with the software.You will be able to access all of the zones—it is representative of a student experience.May also be used with customers for demonstration purposes,but do not hand out the CD.We created a limited quantity for internal use only.This is NOT in JC.Visual overview of the program including teacher and student materials,and software applications.Each edition is highlighted.Includes several lessons from the Teaching Guide.Provides customers with an overview of the entire book and highlights several lessons.We are not providing copies of the complete TG in any e this item for any customers who want to know what's covered in the TG.T otal pages:60.Item #153045159589N/A 0-545-11901-4159292Availability at JCNow!Now!N/A Now!Now!Availability on the PortalNow!N/AN/ANow!N/APromotional Materials and SamplersItem#1602443007/08SCHOLASTIC,SYSTEM 44,SCHOLASTIC PHONICS INVENTORY,and associated logos and designs are trademarks and/or registered trademarks of Scholastic Inc.Other company names,brand names,and product names are the property and/or trademarks of their respective owners*Unit cost based on initial quote.Final cost may differ slightly.R E S E A R C HItemSystem 44Research Foundation PaperSPI Assessment PaperSPI T echnical ManualSystem 44Formative Research PaperDescriptionThorough explanation of the research foundations behind System 44.Underscores the importance of anassessment like SPI and its effectiveness.Details how SPI was validated including the study that correlates SPI to the TOWRE and Woodcock-JohnsonDetails the results of studies conducted during the development of System 44.Item #1595861593280-545-11679-1TKAvailability at JCNow!Now!8/1/089/15/08Availability on the PortalNow!Now!Now!9/1/08P R O M OT I O N A L M AT E R I A L SDescription Includes selections from the Teaching Guide ,Decodable Digest ,44Book ,Student Libraries and T eaching Resources Guide.Also features selections from theScreening ,Assessment and Reporting Guide ,highlighting the System 44and SPI reports.T otal pages:76Features wonderful visual of the sounds and spellings that are taught in the program.Great for hanging during an event or presentation.Item PrintComponent Sampler (Book)The System of Sounds &Spelling PosterItem #159293155626Availability at JCNow!Now!Availability on the PortalN/AN/AO N LY O N T H E S A L E S P O R TA L !Availability on the PortalNow!Now!Now!Now!Now!Now!Now!Now!Now!And distributedon CDN/ADescriptionFor READ 180and non READ 180customers.Compiled list of questions since the launchof System 44.Updated weekly and posted on the portal.PowerPoint®slides outlining the strengths and weaknesses of technology and print competitors.The list of books in the Upper Elementary and Secondary Student Libraries with their corresponding Lexile®levels and genre.For READ 180and non READ 180customers.For READ 180and non READ 180customers.A detailed program scope and sequence.Up-to-date technical specifications.NEW!Includes PowerPoint®presentation with video and script that was presented at the summer sales meeting.An animation of the 44reasons poster that runs on a loop for conferences and other events.ItemOrder FormsFAQsCompetitive OverviewBook ListSales ProposalsCost ProposalsScope &SequenceT echnical SpecificationsSales PresentationConference Exhibit Loop。

User GuideModel 380363Insulation Tester / MegohmmeterIntroductionCongratulations on your purchase of Extech’s Insulation Tester/Megohmmeter. The Model 380363 provides three test ranges plus continuity and AC/DC voltage measurement. Manual datalogging stores up to 9 data sets. This professional meter, with proper care, will provide years of reliable service.Safety1. Circuits under test must be de-energized and isolated before connections are made (except forvoltage measurements).2. Circuit connections must not be touched during a test. Use extreme caution when working nearbare conductors and bus bars. Accidental contact with conductors could result in electricalshock.3. Use caution when working near voltages above 60VDC or 30VACrms.4. After insulation tests, capacitors must be discharged.5. Test leads (including alligator clips) must be in good working order, clean and without brokenor cracked insulation.6. When servicing, use only specified replacement parts.International Safety SymbolsCaution, refer to this manual before using this meterDangerous VoltagesMeter is protected throughout by double or reinforced insulationMeter Description1. LCD Display2. LOCK button3. MEMORY control button4. UP arrow key5. TEST button6. Rotary switch7. DOWN arrow key8. READ memory button9. ZERO adjust button 10. Input terminalsThe tilt stand and battery compartment are located at the rear of the instrumentOperationConnecting Test LeadsFor all measurements connect the red test lead to the V Ω input terminal and the black test lead to the COM input terminal. Test Lead Check 1. Set the rotary switch to the range.2. Touch the test lead tips together.3. Resistance should read less than 0.5Ω and the audio tone should sound.4. With the leads not touching, the display should read OL indicating over-range.5.Readings displayed other than the readings described above are evidence of a problem and the test leads must be replaced before using the meter. Failure to do so could result in damage to equipment and electrical shock. If replacing the test leads does not solve the problem, return the instrument for repair.Insulation Resistance Measurements (Megohmmeter Tests)Warning: Do not perform Insulation Resistance measurements if AC Voltage is present on the device under test.1. Connect the red test lead to the VΩ input terminal; black lead to the COM terminal.2. Set the function switch to the desired MΩ test voltage position.3.will be displayed if the voltage is over 30V.4. The display will show “----“ until the TEST button is pushed. Press and hold the TEST button.The upper right display shows the test voltage applied. The main display shows the resistance.If the display reads OL, the measurement is beyond the range of the instrument.5. Keep the test leads connected to the equipment under test and release the TEST button. Thecircuit will discharge through the meter. Keep the test leads connected until the circuit iscompletely discharged and the upper right display shows 0 volts.Test Lock FunctionFor hands-free operation, use the TEST LOCK feature.1.2. Press the TEST key to start the test. A beeper will sound every 2 seconds to indicate that themeter is in Lock mode.3. Press LOCK or TEST to disable the Lock function and end the test.Notes on IR (Megohmeter) testing:1. The maximum measurement range for the 380363 is 10GΩ. Frequently, insulation resistancewill exceed this value and the meter will show the overload display symbol OL, indicating that the insulation under test is good.2. If the device being tested is highly capacitive, the display will indicate an increasing resistancevalue over time. Allow the reading to stabilize before ending a test.Manual Data Store and Read ModeTo Clear Memory1. Turn the meter off.2. Press and hold down the MEM key, and turn the meter on.3. The display will show “MEM”, "X", "CLr" and "no".4. Press " ” to select "yes" to erase or "no" to leave.5. If "yes" was selected, press "MEM" to erase the memory.Entering data into memory1. Press the MEM key to enter the displayed data into memory.2. The display will show “MEM” and the memory address number in the upper left hand corner.Total memory size is 9 sets.3. When the memory is full, the LCD will show "MEM F".Reading memory data1. Press the READ key to enter Read Mode. The display will show the “READ” icon and thememory address number in the upper left hand corner.2. Use the and arrow keys to scroll through the stored readings.3. Press the READ key again to exit this mode.AC/DC Voltage Measurement1. Set the Rotary switch to the ~V position.2. Connect the red test lead to the VΩ terminal and the black test lead to the COM terminal.3. Connect the test leads to the circuit under test.4. Read the voltage value on the LCD. The meter shows DC voltage in the upper right and ACvoltage on the main display.Resistance Measurement and Audible Continuity Test2. Connect the red test lead to the VΩ terminal and the black lead to the COM terminal.3. Connect the tips of the test leads to the circuit under test.4. Read the resistance value on the display. When the resistance of a circuit is less than approx.100Ω ± 80Ω, the audible tone will sound.LOΩ Low Resistance Measurement1. Set the Rotary switch to the LoΩ position.2. Connect the red test lead to the VΩ terminal and the black lead to the COM terminal.3.4.5. Press and hold the TEST key until a stable reading is displayed.6. Release the TEST key. The upper right hand display will hold the reading until a new test isstarted or a different function is selected.7. Swap the red and black probes to reverse the polarity of the test current. The reading shouldremain the same. This test is useful to detect corroded connections which can cause different readings in both polarities.Using the LOCK Function to measure resistanceThe Lock function can be used to make several resistance measurements in succession without the need to push and hold the TEST key for each measurement.1. Press the LOCK key to enter the LOCK mode, and then press the TEST key.2. Zero out the test lead resistance.3. Connect the probes to the test locations in succession.4. Press the LOCK key to disable the lock function.Note: The meter cannot indicate if the circuit is live in this mode. Ensure that the circuit is de-energized before connecting the test leads or the fuse may blow.Auto Power OffTo conserve battery life, the meter will automatically turn off after 15 minutes of non-use. To turn the meter back on, turn the rotary switch to OFF, then to the desired function.Analog Bar GraphThe analog bar graph displays resistance on a logarithmic scale and voltage on a linear scale. The value always tracks the main display.MaintenanceBattery ReplacementWhen the low battery symbol appears (BT) on the LCD the six 1.5V ‘AA’ batteries must be replaced.1. Turn the meter off and remove the test leads2. Remove the four (4) Phillips head screws on the rear of the meter3. Remove the meter’s rear cover4. Replace the batteries observing polarity5. Affix the rear cover and secure the rear screwsYou, as the end user, are legally bound (Battery ordinance) to return all used batteriesand accumulators; disposal in the household garbage is prohibited!You can hand over your used batteries / accumulators at collection points in yourcommunity or wherever batteries / accumulators are sold!Disposal: Follow the valid legal stipulations in respect of the disposal of the device at theend of its lifecycleFuse ReplacementIf the meter turns ON but does not measure correctly, check the internal fuse.1. Turn the meter off and remove the test leads2. Remove the four (4) Phillips head screws on the rear of the meter3. Remove the meter’s rear cover4. Check and replace the fuse if necessary (0.5A / 600V fast blow)5. Affix the rear cover and secure the rear screwsCleaningPeriodically wipe the case with a dry cloth. Do not use solvents or abrasives to clean this instrument.ApplicationsMeasuring Power Tools and Small AppliancesThis section applies to any device under test that uses a line cord.connected to the device’s housing (chuck, blade, etc.) and to thelines of the power cord. Refer to the diagram.Testing AC MotorsDisconnect the motor from the line by disconnecting the wires from the motor terminals or opening the mains switch.If the mains switch is opened, and the motor also has a motor-starter, then the starter must be held in the ON position. With the mains switch opened, the measured resistance will include the resistance of the motor wire and all other components between the motor and the main switch. If a weakness is indicated, the motor and other components should be checked individually. If the motor is disconnected at the motor terminals, connect one meter lead to the grounded motor housing andthe other lead to one of the motor leads. Refer to diagram at below.Testing DC Motors1. Disconnect the motor from the line.2. To test the brush rigging, field coils and armature, connect one meter lead to the groundedmotor housing and the other lead to the brush on the commutator.3. If the resistance measurement indicates a weakness, raise the brushes off of the commutatorand separately test the armature, field coils and brush rigging (one at a time). Leave one lead connected to the grounded motor housing while testing the motor components. This alsoapplies to DC Generators.Testing Cables1. Disconnect the cable under test from the line.2. Disconnect the opposite end of the cable to avoid errors as a result of leakage from otherequipment.3. Check each conductor to ground and/or lead sheath by connecting one meter lead to groundand/or lead sheath and the other meter lead to each of the conductors in turn.4. Check insulation resistance between conductors by connecting meter leads to conductors inpairs. Refer to diagram. In the diagram, note that the 3-conductor cable has two wires shorted to the ground shield. This two-wire/shield connection is then connected to one side of themeter. The remaining conductor is connected to the other side of the meter.SpecificationsGeneral specificationsDisplay 3-3/4 digit (4000 count) LCDSampling rate 1 reading per secondTest ranges Resistance: 4MΩ, 40MΩ, 400MΩ, 4000MΩ, 10GΩTest Voltages: 250V, 500V, 1000VAC/DC Voltage range 999VResistance range 9999ΩLo Resistance range 40ΩOver range indicator ‘OL’ displayed when measurement exceeds range Zero adjust AutomaticMemory Nine (9) data recordsLow battery indicator ‘BT’ symbol displayed when battery voltage is low Power source Six (6) 1.5 ‘AA’ batteriesPower consumption 20 to 95mA (depending upon function)Fuse protection 0.5A / 600V fast blow fuseOperating conditions 0 to 50o C (32 to 122o F); 80% RHDimensions 235 x 116 x 54mm (9.3 x 4.6 x 2.1”)Weight 520g (1.15 lbs)Safety ratings Pollution degree 2; indoor use; CE marked;Meets IEC 61010-1 and IEC 61557 standards Category Rating CAT III-1000VRange SpecificationsMEGOHMMETER RANGES Range Test Voltages Resolution Accuracy4M Ω 0.001M Ω40M Ω 0.01M Ω 400M Ω 0.1M Ω4000M Ω 1M Ω10G Ω 250V (+30% ~ -0%) 500V (+30% ~ -0%)1000V (+30% ~ -0%)00.01G Ω 3%+5 (<1000M)5%+5 (>1000M)(% reading + digits at 23o C ± 5oC < 80% RH)Analog Bar Graph 0 to 10G Ω Nominal Current ≧1mACircuit ProtectionTest inhibited if input ≧ 30V AC or DCAC VOLTAGE (40Hz-500Hz)Range Res. Accuracy Input impedance OverloadProtection999VAC1V±2% + 2d9M Ω1000VrmsDC VOLTAGERange Res. Accuracy Input impedance OverloadProtection999VDC1V±1% + 2d9M Ω1000VrmsRESISTANCE & CONTINUITYRangeRes. Accuracy Max. open circuitVoltOverload Protect. Resistance: 999.9Ω 0.1Ω±1% + 3d3V1000VrmsContinuity100Ω ± 80Ω)LOW RESISTANCE Ω Range Res.Accuracy Max. open circuitVoltOverload Protection 40Ω 0.01Ω±2% + 2d6V>30V AC/DCCopyright © 2012 Extech Instruments Corporation (a FLIR company)All rights reserved including the right of reproduction in whole or in part in any form.。

88 Insulation tester ideal for maintenance and repair operations on electrical equipment such as motors, cables, etc.65290.010MΩ to 420.0MΩ0.020MΩ to 420.0MΩ0.050MΩ to 4200MΩFEATURES• Selectable test voltages 250, 500 and 1000V (model 6528)• Selectable test voltages 50, 100, 250, 500 and 1000V (model 6529)• Basic DMM functions; Volts, Continuity, Resistance • DAR and PI ratio test functions (model 6529)• Dual line display to view the insulation value and real-time test voltage simultaneously in an easy-to-read format• DMR mode - relative resistance comparison to a reference value• 2-color backlighting easily shows alarm conditions • Shockproof sheath for excellent handling • Automatic power-off function to optimize the battery life• Programmable alarm thresholds2126.55Megohmmeter Model 6529 (Digital, 50V, 100V, 250V, 500V, 1kV, 420k-Ohm, Continuity, Alarm, Timer & PI/DAR)PRODUCTS INCLUDEIncludes soft carrying case, set of (2) 5 ft color-coded (red/black) silicone leads and alligator clips, (2) color-coded (red/black) test probes {Rated 1000V CAT IV, UL V2}, (6) 1.5V AA batteries and a user manual.ACCESSORIES/REPLACEMENTS65296528Catalog #2138.54 Continuity probe Catalog #2119.02 Soft carrying pouch Catalog #2971.04 Fuse – Set of (2) FF, 200mA, 1000V, 10kA, 6x32mmCatalog #5000.94Lead – Set of (2) 5 ft color-coded (red/black) silicone leads with 4mm straight/right angle banana plugs (Rated 1000V CAT IV, UL)Catalog #5000.97/#5000.98 Black/Red Test Probe (1000V CAT IV, 15A, UL V2)Cat #5000.99/#5100.00 Clip – Safety Alligator – Black/Red (1000V CAT IV, 15A, UL V2)N E WUS Design Patent US D890,617 S。

DEAE-纤维素1 简介DEAE-纤维素，它采用平均粒径为50μm的颗粒型亲水高分子聚合物，表面又用大分子糖链接枝，使它有更高的比表面积和更好的生物兼容性，保持更高载量，同时又具有更好的分辨率。

由于比表面积大，平衡和洗脱的时间也更短。

它经过接枝即使是纯化病毒，质粒等超大分子的物质，载量基本保持不变。

本产品物理和化学稳定性好，使用寿命长，操作方便。

2 填料特征：3 应用的注意事项：3.1 色谱柱装填（1）所需要用到的材料的温度要与色谱操作的温度一样，液体最好做脱气处理。

填料可直接称量需要的量用缓冲液溶涨一小时装柱即可。

（2）在柱子下端加入20%乙醇，以除去柱子中的空气，关闭柱子出口，在柱内保留少量的20%乙醇。

20%乙醇容易产生气泡，可以在里面加1%吐温避免气泡产生。

也可以换成纯水装柱子，但是需要把填料中的20%乙醇也换成纯水，具体的方法取需要体积的填料在抽滤漏斗上进行，也可以小心倾去填料上的20%乙醇，再换成5倍体积的纯水，反复沉淀去上清，5次左右就可以用于装柱子。

（3）此填料颗粒比较细，所以一定要注意柱子要选择合适的筛网，不能漏，也可以取点填料加到筛网上试试，如果没有问题再将填料连续倒入柱子时，要用玻璃棒的紧靠柱子内壁引流，以减少气泡的产生，让填料先自然沉降到填料体积不再变化，而填料和上面的液体很好分层，上层溶液完全澄清，就可以开泵用适当的流速压柱子，填料体积不再变化后，再把转换头紧顶在填料上就可以平衡柱子使用。

使用的流速要小于装柱子的流速。

（4）在装柱子前，填料从冰箱中取出至少要室温放置2-3个小时，这样避免装柱子时由于温度变化而使柱子中产生气泡。

3.2 蛋白的结合样品的盐浓度和pH要尽量和平衡柱子的缓冲液一致，盐浓度过高或者pH带低也许挂不上，所以要根据自己的样品做适当调整。

3.3 蛋白的洗脱这个填料如果采用线性梯度洗脱，柱子的直径和高度比最好是大于10，数值越大越有利于分离，而且样品最好别上太多，可以按约10mg/ml上样，如果采用阶段洗脱的方法，装短粗柱子就可以，上样量也没有限制。