现代仪器分析 第一讲_ 高效毛细管电泳法在药物分析中的应用chaj ppt课件

毛细管电泳分析法在药物分析中的应用摘要毛细管电泳技术又称为高效毛细管电泳。

作为一种新的分离分析技术，以其高效，快速，低实验消耗等优点，受到了广泛重视，而其在药物分析中的应用得到迅速的发展。

在原料分析中的中药材鉴别和质量控制，中药有效成分的分离与测定和中成药制剂。

而在西药复方制剂中，广泛用于解热镇痛药、抗组胺药、消炎药和止咳药，降压药和抗生素、合成抗菌剂及生物技术产品等药物和制剂的分离，鉴定和分析及其对手性分子的拆分，基于手性主—客体络合的毛细管电泳手性拆分，基于手性胶束增溶的毛细管电泳手性拆分和基于蛋白质亲和的毛细管电泳手性拆分，还有临床用药中，都显示了其高效，快速的特点。

毛细管电泳技术正广泛用于药物分析的各个相关的部分中，正越来越受到人们的重视。

AbstractCapillary electrophoresis technology called high performance capillary electrophoresis. As a new kind of separation and analysis technology, with its rapid, efficient, low consumption advantages of experiment was Received widely attention, and its application in pharmaceutical analysis is rapid development. The analysis of Chinese herbal medicine in raw material identification and quality control, the TCM separation and determination and proprietary Chinese medicine preparations. But in western medicine compound preparations, widely used in antipyretic analgesics, the antihistamine drugs, expectorant and cough, antihypertensives and antibiotics, synthetic antibacterial agent and biotechnology product such drugs and preparation of separation, appraisal and analysis and the opponent of chiral molecule split, based on chiral Lord - object complexation of capillary electrophoresis chiral resolution, based on chiral dissociation of increase soluble adopted capillary electrophoresis chiral separation and based on protein affinitive capillary electrophoresis chiral resolution, and clinical medicine, shows its high efficiency, fast characteristic. Capillary electrophoresis technology is widely used in pharmaceutical analysis of each relevant sections, are becoming more and more attention by people.关键词：毛细管电泳技术药物分析应用Keywords: Capillary electrophoresis drug analysis application前言毛细管电泳(CE) 又称为高效毛细管电泳(high performance capillary electrophoresis，HPCE)，它以弹性石英毛细血管为分离通道，以高压直流电场为驱动力，依据样品中各组分的淌度和分配行为上的差别进行分离和分析。

毛细管电泳分析法在药物分析中的应用摘要：毛细管电泳可以分离无机离子、有机离子、小分子和大分子等物质，在生命科学、化学、药学等领域得到了广泛的应用。

随着HPCE技术进入成熟阶段和日益普及，利用其优势特点来解决中药有效成分或特征成分是一种发展的趋势；HPCE可以应用于西药各种剂型中主要成分的测定，可较少许多程序；HPCE课取代微生物效价法来测定抗生素的含量测定；此外在中药的含量测定也会起到强大的力量。

毛细管电泳的其他分离模式也使其优势更为明显，在药物分析中有着一个举足轻重的地位。

Capillary electrophoresis can Separates Inorganic Ions、organic ions、Macromolecule、Small Molecular、and so on.It has found wide applications in life science、chemistry 、Pharmacy and other relative areas.since the de and public use of HPCE technology ,using it’s advantange to solve chinese medicine effective constituents or characteristic components has became the development tendency, HPCE can apply to the determination of main Composition in various Formulation of western medicine which may decrease many procedures . what is more HPCE class determinates the content in antibiotics instead of microbiological assay of antibiotics.In additions ,italso play an important role in the determination of chinese medicine. The other separation about Capillary electrophoresis are put it into a more high level and occupies a decisive position in the pharmaceutical analysis.关键字：高效毛细管电泳、药学药品分析、中药分析、抗生素含量测定、杂质含量测定毛细管电泳，也称高效毛细管电泳（HPCE），是以高压直流电场为驱动力，以毛细管为分离通道，根据样品中各组份的淌度和分配行为的不同进行分离的一种分析方法，是近年来迅猛发展的分析分离技术，进行高效快速分离的一种新技术。