Ezetimibe_163222-33-1_DataSheet_MedChemExpress

2.1. Classification of the substance or mixture Regulation (EC) No 1272/2008: -Directive 67/548/EEC: -2.2. Label elementsClassification according to Regulation (EC) No. 1272/2008 [CLP] Hazard pictograms:-Signal word: -Hazard statements: -Precautionary statements: -Classification according to Directive 67/548/EEC or 1999/45/EC Hazard symbols:-R-phrases: -S-phrases: -2.3. Other hazardsNo risks worthy of mention.SECTION 3: Composition / information on ingredients 3.1. SubstancesNatural plant extract.3.2. Mixturesnot applicableComposition/information on ingredientsSubstance: EC-no.: CAS-No.: INDEXno.: REACH-no.: Concentration: Classification: EC1272/2008(CLP):Classification:67/548/EEC:Glycerides, Limnanthes alba 604-884-4 153065-40-8,169107-13-5≤ 100 (w/w)Substance with a common (EC) occupational exposure limit valueSubstance: EC-no.: CAS-No.: INDEX no.: REACH-no.: Concentration: Classification: EC1272/2008(CLP): Classification: 67/548/EEC:(Full text of R-, H- and EUH-phrases: see section 16.)Additional informationnoneSECTION 4: First aid measures4.1. Description of first aid measuresGeneral information: In case of accident or if you feel unwell, seek medical advice immediately(show safety data sheet if possible). Take off immediately all contaminatedclothing.Following inhalation: Move victim to fresh air. Put victim at rest and keep warm. Seek medicalattention if problems persist.Following skin contact: After contact with skin, wash immediately with plenty of water and soap. Incase of skin irritation, seek medical treatment.After eye contact: No special measures are necessary. In case of eye irritation consult anophthalmologist.After ingestion: Rinse mouth immediately and drink large quantities of water. Seek medicalattention if problems persist.Self-protection of the first aider: No special measures are necessary.4.2. Most important symptoms and effects, both acute and delayedSymptoms: No symptoms known up to now.Nature of Hazard: No risks worthy of mention.4.3. Indication of any immediate medical attention and special treatment needed Notes for the doctor: Have victim drink large quantities of water, with active charcoal if possible. Special treatment: First Aid, decontamination, treatment of symptoms.SECTION 5: Firefighting measures5.1. Extinguishing mediaSuitable extinguishing media: Carbon dioxide (CO2). Dry extinguishing powder. Foam.Unsuitable extinguishing media: Water fog. High power water jet. Water.5.2. Special hazards arising from the substance or mixturePyrolysis products, toxic. Carbon dioxide. Carbon monoxide.5.3. Advice for firefightersGeneral informationMove undamaged containers from immediate hazard area if it can be done safely.Special protective equipment for firefighters:In case of fire: Wear self-contained breathing apparatus.Additional informationCollect contaminated fire extinguishing water separately. Do not allow entering drains or surface water.SECTION 6: Accidental release measures6.1. Personal precautions, protective equipment and emergency proceduresWear personal protection equipment. Guide people to safety. Provide adequate ventilation. Remove all sources of ignition. High slip hazard because of leaking or spilled product.6.2. Environmental precautionsDo not empty into drains or the aquatic environment. Do not allow to enter into soil/subsoil.6.3. Methods and material for containment and cleaning upAbsorb with liquid-binding material (e.g. sand, diatomaceous earth, acid- or universal binding agents). Treat the assimilated material according to the section on waste disposal. Avoid generation of dust. Clear contaminated area thoroughly.6.4. Reference to other sectionsSafe handling: see section 7Personal protection equipment: see section 8Disposal: see section 13Crafter's Choice™ Meadowfoam Seed Oil Revision date: 28.05.2015SECTION 7: Handling and storage7.1. Precautions for safe handlingAdvices on safe handlingAvoid contact with eyes.Technical measuresProvide for sufficient ventilation and punctiform suction at critical points.Precautions against fire and explosionUsual measures for fire prevention.Additional informationnone7.2. Conditions for safe storage, including any incompatibilitiesTechnical measures and storage conditionsNo special technical protective measures are necessary.Packaging materialsKeep/Store only in original container.Requirements for storage rooms and vesselsKeep/Store only in original container. Keep container tightly closed.Hints on joint storageDo not store together with: Oxidizing agents. Keep away from food, drink and animal feedingstuffs. Storage class: 10 Combustible liquids unless LGK 3A or 3B.Further information on storage conditionsProtect against: heat. UV-radiation/sunlight.7.3. Specific end use(s)Observe instructions for use.SECTION 8: Exposure controls/personal protection8.1. Control parametersoccupational exposure limit valueSubstance: CAS-No.: Source: Occupationalexposure limit value: Limitation ofexposure peaks:Remark:Substance with a common (EC) occupational exposure limit valueSubstance: CAS-No.: Source: Occupationalexposure limit value: Limitation ofexposure peaks:Remark:DNEL-/PNEC-valuesDNEL valueSubstance: CAS-No.: DNEL/DMEL Industry Professional consumer PNEC ValueSubstance: CAS-No.: PNEC Worker, industry Worker, professional consumer Risk management measures according to used control banding approachnoneCrafter's Choice™ Meadowfoam Seed Oil Revision date: 28.05.2015 Additional informationDoes not contain substances above concentration limits fixing an occupational exposure limit.8.2. Exposure controlsOccupational exposure controlsTechnical measures and the application of adequate working methods take priority over the use of personal protection equipment.General protection and hygiene measuresDo not eat, drink, smoke or sneeze at the workplace. Wash hands before breaks and at the end of work. Apply skin care products after work. Wash contaminated clothing prior to re-use.Personal protection equipmentMinimum standard for preventive measures while handling with working materials are specified in the TRGS 500.Respiratory protectionRespiratory protection not required.Hand protectionTested protective gloves are to be worn: German Industry Norms (DIN) / European Norms (EN): DIN EN 374Eye/face protectionTightly sealed safety glasses.Body protectionFor the protection against direct skin contact, body protective clothing is essential (in addition to the usual working clothes). Only wear fitting, comfortable and clean protective clothing. Barrier creams are not substitutes for body protection.Environmental exposure controlsrefer to chapter 7. No further action is necessary.Consumer exposure controlsrefer to chapter 7. No further action is necessary.Exposure ScenariononeSECTION 9: Physical and chemical properties9.1. Information on basic physical and chemical propertiesAppearancePhysical state: liquidColour: yellow - goldenOdour: characteristic like: oil.Odour threshold: No data availableSafety relevant basis dataparameter unit remarkDensity: 0,90-0,92 g/cm³Bulk density: not applicablepH: not applicableMelting point/freezing point: 5-7 °CInitial boiling point and boiling No data availableCrafter's Choice™ Meadowfoam Seed Oil Revision date: 28.05.2015 range:Flash point: 334 °CFlammability (solid, gas): No data available Explosivity: No data available Lower explosion limit: No data availableUpper explosion limit: No data availableIgnition temperature: No data available Decomposition temperature: No data availableOxidizing potential: not applicableVapour pressure: No data availableVapour density: No data available Evaporation rate: not applicableWater solubility: not miscibleFat solubility: complete miscibleSoluble in: : not applicablePartition coefficient: n-No data availableoctanol/water:Viscosity: at °C: 40 49,6 cStSolvent separation test: No data availableSolvent content: No data available9.2. Other informationnoneSECTION 10: Stability and reactivity10.1. Reactivitynone10.2. Chemical stabilityWith proper storage and handling the product is stable.10.3. Possibility of hazardous reactionsnone10.4. Conditions to avoidheat. UV-radiation/sunlight.10.5. Incompatible materialsOxidizing agents.10.6. Hazardous decomposition productsPyrolysis products, toxic. Carbon monoxide. Carbon dioxide.Additional informationnoneSECTION 11: Toxicological information11.1. Information on toxicological effectsNo information available.Acute toxicitySubstance: CAS-No.: Toxicological informationCrafter's Choice™ Meadowfoam Seed Oil Revision date: 28.05.2015 Specific symptoms in laboratory animalsNo information available.Irritation and etchingIrritant effect on the skinNot an irritant.Irritant effect on the eyeNot an irritant.Irritant effect on the respiratory tractNot an irritant.Additional informationnot corrosiveSensitizationno danger of sensitization.Repeated dose toxicity (subacute, subchronic, chronic)No information available.CMR effects (carcinogenicity, mutagenicity and toxicity for reproduction)CarcinogenicityNo indications of human carcinogenicity exist.Germ cell mutagenicityNo indications of human germ cell mutagenicity exist.Reproductive toxicityNo indications of human reproductive toxicity exist.General remarksnonePractical experience/human evidencenoneOther observationsnoneOther informationnoneSECTION 12: Ecological information12.1. ToxicityNo information available.EcotoxicitySubstance: CAS-No.: Ecotoxicity12.2. Persistence and degradabilityEasily biodegradable (concerning to the criteria of the OECD)12.3. Bioaccumulative potentialNo indication of bio-accumulation potential.12.4. Mobility in soilnone12.5. Results of PBT and vPvB assessmentThis substance does not meet the criteria for classification as PBT or vPvB.12.6. Other adverse effectsnoneFurther ecological informationDo not empty into drains or the aquatic environment. Do not allow to enter into soil/subsoil.Further detailsnoneSECTION 13: Disposal considerations13.1. Waste treatment methodsAppropriate disposal/ProductWaste disposal according to EC Directives 75/442/EEC and 91/689/EEC on waste and hazardous waste in their latest versions.Appropriate disposal / PackageCleaned containers may be recycled. Handle contaminated packaging in the same way as the substance itself.List of proposed waste codes/waste designations in accordance with EWCAccording to EAKV, allocation of waste identity numbers/waste descriptions must be carried out in a specific way for every industry and process.Waste code product: 20 01 26 - oil and fat other than those mentioned in 20 01 25Waste code packaging: 15 01 06 - mixed packagingremarkWaste disposal according to official state regulations.SECTION 14: Transport information14.1. UN numberUN No.: none14.2. UN proper shipping nameOfficial directive for the transportnonenoneProper Shipping namenonenone14.3. Transport hazard class(es)Hazard label(s): none Classification code:none14.4. Packing groupPacking group: none14.5. Environmental hazardsEnvironmental hazards: none14.6. Special precautions for userLand transport (ADR/RID)Remark: Not a hazardous material with respect to these transportation regulations.Transport category: none Tunnel restriction code: noneSpecial provisions: none Limited quantity (LQ): noneSea transport (IMDG)Special Provisions: noneRemark: Not a hazardous material with respect to these transportation regulations.EmS-No: none MFAG:none Marine pollutant: noneSpecial provisions: none Limited quantity (LQ): noneAir transport (ICAO-TI / IATA-DGR)Remark: Not a hazardous material with respect to these transportation regulations.Limited quantity (LQ): none14.7. Transport in bulk according to Annex II of MARPOL 73/78 and the IBC CodeRemark: noneSECTION 15: Regulatory informationLabelingHazardous component(s) for labellingGlycerides, Limnanthes albaSpecial labelling of particular preparationsSafety data sheet available for professional user on request.15.1. Safety, health and environmental regulations/legislation specific for the substance or mixtureEU legislationInformation according to 1999/13/EC about limitation of emissions of volatile organic compounds (VOC-guideline).noneRegulation (EC) No 2037/2000 concerning materials, which cause damage to the ozone layer.noneRegulation (EC) No. 648/2004 (Detergents regulation)noneNational regulationsObserve in addition any national regulations!Restrictions of occupationnoneStorage class10 Combustible liquids unless LGK 3A or 3B.Water hazard class (WGK)1 weak water pollutant (WGK 1)Other regulations, restrictions and prohibition regulationsCrafter's Choice™ Meadowfoam Seed Oil Revision date: 28.05.2015 none15.2. Chemical Safety AssessmentFor this preparation a chemical safety assessment has been carried out: -For this substance a chemical safety assessment is not required.SECTION 16: Other informationRelevant R-, H- and EUH-phrases (Number and full text)Hazard statements-R-phrases-Training advicenoneRecommended restrictions of userefer to chapter 1.Further remarksThe above information describes exclusively the safety requirements of the product and is based on our present-day knowledge. The information is intended to give you advice about the safe handling of the product named in this safety data sheet, for storage, processing, transport and disposal. The information cannot be transferred to other products. In the case of mixing the product with other products or in the case of processing, the information on this safety data sheet is not necessarily valid for the new made-up material.。

Inventec Performance Chemicals USA, LLCSAFETY DATA SHEET (SDS)SECTION 1: PRODUCT AND COMPANY IDENTIFICATIONPRODUCT NAME: Amtech Tacky Paste Flux Series: 200, 400, 500, 600, 4000, SynTECH, WSFC-305L and #61 SYNONYMS:Tacky FluxMANUFACTURER: Inventec Performance Chemicals USA, LLCADDRESS:PO Box 989 Deep River, CT 06417 USAPHONE:860-526-8300FAX:860-526-8243EMERGENCY:Infotrac-(800)535-5035REVISION DATE:December 19, 2014REVISION DATE: 3DOCUMENT NAME:SDS-Tacky Flux-008PRODUCT USE:Bonding solder joints in production and repair of circuit boardsSECTION 2: HAZARDS IDENTIFICATIONCHEMICAL NAME:N/ACHEMICAL FAMILY:MixtureCHEMICAL FORMULA:N/AROUTES OF ENTRY: Inhalation, Ingestion, Skin/Eye ContactGHS:Signal Word: WarningHazard statement(s)H302 Harmful if swallowedH317 May cause an allergic skin reactionH320 Causes eye irritationH335 May cause respiratory irritationPrecautionary statement(s)P102 Keep out of reach of childrenP233 Keep container tightly closedP264 Wash hands thoroughly after handlingP270 Do not eat, drink or smoke when using this productP280 Wear protective gloves/protective clothing/eye protection/face protectionP302+P352 IF ON SKIN: Wash with plenty of soap and waterP305+P351 IF IN EYES: Rinse continuously with water for several minutesP404 Store in a closed containerP501 Dispose of contents/containers in accordance with Federal, State/Provincial, and/or local regulations POTENTIAL HEALTH EFFECTS:EYE CONTACT: May cause moderate irritation. Do not allow material to come in contact with eyes.SKIN CONTACT: May cause moderate skin irritation.INHALATION: May cause irritation to the respiratory tract.INGESTION: Harmful if swallowed. May cause irritation to the mouth, throat, and stomach. May cause abdominal discomfort, nausea, vomiting, and/or diarrhea.CHRONIC: Not established.SECTION 2 NOTES:Inventec Performance Chemicals USA, LLC does not recommend, manufacture, market, or endorse any of its products for human consumption.SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTSIngredient CAS Number Exposure LimitsModified Rosins N/A N/APine Oil Derivatives 8000-41-7 N/AProprietary Ingredients N/A N/AMixed Carboxylic Acids N/A N/ASECTION 3 NOTES:Percentages of individual components are not listed as this information is considered a trade secret.SECTION 4: FIRST AID MEASURESEYES: Flush with plenty of water, contact a physician. If contact lenses can be removed easily, flush eyes without contact lenses. SKIN: Wash affected area with plenty of warm, soapy water. If irritation persists, seek medical attention.INGESTION: Call a physician or Poison Control Center immediately. Do not induce vomiting.INHALATION: Remove to fresh air. If not breathing, seek immediate medical attention.SECTION 5: FIRE-FIGHTING MEASURESEXTINGUISHING MEDIA: Dry chemical, foamSPECIAL FIRE FIGHTING PROCEDURES: Do not use water. Use NIOSH-approved self-contained Breathing Apparatusand full protective clothing if involved in a fire.UNUSUAL FIRE AND EXPLOSION HAZARDS:This product does not present any unusual fire and explosion hazards. SECTION 6: ACCIDENTAL RELEASE MEASURESACCIDENTAL RELEASE MEASURES: If material spills or leaks, collect and place into a properly labeled waste container. Remove traces of tacky flux using cloth rags or paper towels moistened with Isopropyl Alcohol. Follow on-site personal protective equipment recommendations.SECTION 6 NOTES:See Sections 2, 4, and 7 for additional information.SECTION 7: HANDLING AND STORAGEHANDLING/STORAGE: Keep containers tightly closed when not in use. Use care to avoid spills. Avoid inhalation of fumes or dust. Avoid contact with eyes, skin, and clothing.OTHER PRECAUTIONS: Empty containers may retain product residues in vapor, liquid, and/or solid form. All labeled hazard precautions should be observed.WORK HYGIENIC PRACTICES: Cosmetics/Food/Drink/Tobacco should not be consumed or used in work areas. Always wash hands after handling material and before applying or using cosmetics/food/drink/tobacco.SECTION 7 NOTES:For industrial use only.SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTIONVENTILATION: Provide sufficient mechanical (general and/or local exhaust) ventilation to maintain exposure below TLVs. RESPIRATORY PROTECTION: Use with adequate ventilation.EYE PROTECTION: Use with appropriate safety glasses.SKIN PROTECTION: Protective gloves and clothing should be worn when handling material. Wash hands thoroughly with soap and water upon leaving the work area.SECTION 9: PHYSICAL AND CHEMICAL PROPERTIESAPPEARANCE: Clear, White, or Yellow to Dark Amber gelODOR: Mild odorODOR THRESHOLD: Not establishedpH as SUPPLIED: N/ASECTION 9: PHYSICAL AND CHEMICAL PROPERTIES (continued)MELTING POINT: Not establishedFREEZING POINT: Not establishedINITIAL BOILING POINT: Not establishedBOILING RANGE: Not establishedFLASH POINT: Not establishedEVAPORATION RATE: Not establishedFLAMMABILITY (solid): Not establishedUPPER/LOWER FLAMMABILITY: Not establishedUPPER/LOWER EXPLOSIVE LIMITS:Not establishedVAPOR PRESSURE (mmHg): N/A (°F/°C)VAPOR DENSITY (AIR = 1): N/A (°F/°C)RELATIVE DENSITY: Not establishedSOLUBILITY IN WATER: PartiallyPARTITION COEFFICIENT (n-octanol/water): Not establishedAUTOIGNITION TEMPERATURE: Not establishedDECOMPOSITION TEMPERATURE: Not establishedVISCOSITY: N/A (°F/°C)SECTION 10: STABILITY AND REACTIVITYSTABILITY: StableCONDITIONS TO AVOID (STABILITY): Freezing temperatures. High temperatures. INCOMPATIBILITY (MATERIAL TO AVOID): Strong oxidizing materialsHAZARDOUS DECOMPOSITION/BY-PRODUCTS: Harmful organic fumes and toxic oxide fumes may form at elevatedtemperatures.POSSIBILITY OF HAZARDOUS REACTIONS: Will not occurSECTION 11: TOXICOLOGICAL INFORMATIONACUTE TOXICITY: Not availableSKIN CORRISION/IRRITATION: Not establishedSERIOUS EYE DAMAGE/IRRITATION: Not availableRESPIRATORY OR SKIN SENSITIZATION: Not establishedGERM CELL MUTAGENICITY: Not availableCARCINOGENICITY: Not availableREPRODUCTIVE TOXICITY: Not availableSTOT-SINGLE EXPOSURE: Not availableSTOT-REPEATED EXPOSURE: Not availableASPIRATION HAZARD: Not availableSECTION 12: ECOLOGICAL INFORMATIONTOXICITY: Product not testedPERSISTENCE AND DEGRADIBILITY: Product not testedBIOACCUMULATIVE POTENTIAL: Product not testedMOBILITY IN SOIL: Product not testedOTHER ADVERSE EFFECTS: Product not testedSECTION 13: DISPOSAL CONSIDERATIONSWASTE DISPOSAL METHOD: Scrap and waste solder should be stored in a dry, sealed container for later disposal. Disposal must be in accordance with Federal, State/Provincial, and Local Regulations.SECTION 14: TRANSPORT INFORMATIONTransport in accordance with applicable regulations and requirements.UN Number: Not availableUN Proper Shipping Name: Not availablePackaging Group:Not applicableEnvironmental Hazards:NoneTRANSPORT HAZARD CLASSES:US DOT Hazardous Material Classification: Tacky Flux is not listed as a DOT hazardous materialWater Transportation: Tacky Flux is not listed as a hazardous materialIATA Hazardous Material Classification: Tacky Flux is not listed as IATA hazardous materialSECTION 15: REGULATORY INFORMATIONAll ingredients used to manufacture this product are listed on the EPA TSCA Inventory.U.S. FEDERAL REGULATIONS: Not regulatedSTATE REGULATIONS: Not regulatedINTERNATIONAL REGULATIONS: Not regulatedSECTION 16: OTHER INFORMATIONHMIS Rating: Health=1 Flammability=1 Physical Hazard=0 Personal Protection=X KEY:N/A: Not applicableGHS: Global Harmonized SystemOSHA: Occupational Safety and Health AdministrationACGIH: American Conference of Governmental Industrial HygienistsNTP: National Toxicology ProgramIARC: International Agency for Research on CancerCAS: Chemical Abstract ServiceNIOSH: National Institute for Occupational Safety & HealthSTOT: Specific target organ toxicityTLV: Threshold limit valueUS DOT: United States Department of TransportationDOT: Department of TransportationIATA: International Air Transport AssociationEPA:Environmental Protection AgencyTSCA:Toxic Substance Control ActHMIS:Hazardous Material Identification SystemPREPARATION INFORMATION:This update supersedes all previously released documents.PREPARED BY: Wendy W. GesickAPPROVED BY: Leigh W. GesickDISCLAIMER:The information contained herein is based on data considered to be accurate but does not purport to be all-inclusive and shall be used only as a guide. No warranty is expressed or implied regarding the accuracy of this data and Inventec Performance Chemicals USA, LLC shall not be held liable for any damage resulting from any handling or contact with the above product. Liability is expressly disclaimed for loss or injury arising out of use of this information or the use of any materials designated. This material is not for resale, unauthorized distribution, or personal use.。

人核基质蛋白-22ELISA检测试剂盒使用说明书提供商：上海乔羽生物科技有限公司本试剂仅供研究使用标本：尿液试验原理：NMP-22试剂盒是固相夹心法酶联免疫吸附实验（ELISA）.已知NMP-22浓度的标准品、未知浓度的样品加入微孔酶标板内进行检测。

先将NMP-22和生物素标记的抗体同时温育。

人核基质蛋白-22ELISA检测试剂盒洗涤后，加入亲和素标记过的HRP。

再经过温育和洗涤，去除未结合的酶结合物，然后加入底物A、B，和酶结合物同时作用。

产生颜色。

颜色的深浅和样品中NMP-22的浓度呈比例关系。

自备材料蒸馏水。

加样器：5ul、10ul、50ul、100ul、200、500ul、1000ul。

振荡器及磁力搅拌器等。

安全性避免直接接触终止液和底物A、B。

一旦接触到这些液体，请尽快用水冲洗。

实验中不要吃喝、抽烟或使用化妆品。

不要用嘴吸取试剂盒里的任何成份。

操作注意事项试剂应按标签说明书储存，使用前恢复到室温。

稀稀过后的标准品应丢弃，不可保存。

实验中不用的板条应立即放回包装袋中，密封保存，以免变质。

不用的其它试剂应包装好或盖好。

不同批号的试剂不要混用。

保质前使用。

使用一次性的吸头以免交叉污染，吸取终止液和底物A、B液时，避免使用带金属部分的加样器。

使用干净的塑料容器配置洗涤液。

使用前充分混匀试剂盒里的各种成份及样品。

洗涤酶标板时应充分拍干，不要将吸水纸直接放入酶标反应孔中吸水。

底物A应挥发，避免长时间打开盖子。

底物B对光敏感，人核基质蛋白-22ELISA 检测试剂盒避免长时间暴露于光下。

避免用手接触，有毒。

实验完成后应立即读取OD值。

加入试剂的顺序应一致，以保证所有反应板孔温育的时间一样。

按照说明书中标明的时间、加液的量及顺序进行温育操作。

样品收集、处理及保存方法血清-----操作过程中避免任何细胞刺激。

使用不含热原和内毒素的试管。

收集血液后，1000×g离心10分钟将血清和红细胞迅速小心地分离。

1160-006AM040322BHTI Alternate Materials List2299-947-100-690115BHTI Procurement Specification for Epoxy Adhesive, Heat Resistant3299-947-320-820507BHTI Adhesive Film and Primer System, Intermediate Cure Temperature (260-290º F) Service Temperature 67-225º F)468A900000G011101BAC Finish Spec: F-15574A900000E990308BAC Finish Specification for F18 Aircraft674A900004DG M051220BAC Ctrl: Fract Crit Parts, F-18774A901001F981208BAC Std Finish Codes: F-18 A\C8901-947-002CA D950510BHTI Finish Specification for the V-22 Aircraft (Bell Boeing) Model 901) EMDAircraft9A-A-208See Special Notes DL C120719FED Ink, Marking, Stencil, Opaque10A-A-2962Cancelled - no s/s spec A980810Cancel Notice2FED Commercial Item Description Enamel, Alkyd, Exterior, Solvent Based, Low VocOk to use cancspec.11A-A-3097DC-970506Notice 4FED Commercial Item Description Adhesives, Cyanoacrylate, Rapid RoomTemperature-Curing, Solventless12A-A-3165DE A071116FED Lacquer, Gloss, for A/C Use13A-A-00208See Special Notes DL D121001FED Commercial Item Description Ink, Marking, Stencil, Opaque (Porous andNon-Porous Surfaces)14A-A-52080B980523Notice 1FED Tape, Lacing, and Tying, Nylon15A-A-52081DF B980523Notice 1FED Tape, Lacing, and Tying, Polyester16A-A-52082DC D090817FED Tape, Lacing and Tying, TFE-Fluorocarbon17A-A-52083BJ C040223FED Tape, Lacing, and Tying, Glass18A-A-52084B980523Notice 1FED Tape, Lacing and Tying, Aramid19A-A-55829DC A110322DLA Acetic Acid, Glacial, Technical20A-A-56032CN D030521Notice 1FED Commercial Item Description (CIDS) Ink, Marking, Epoxy Base21A-A-59126-970926FED Terminals, Feedthru (Insulated) and Terminals, Stud (Insulated andNoninsulated)22A-A-59132CR A100607ValidationNotice 1DLA Amyl Acetate, Technical23A-A-59135CR-971028FED Commercial Item Description Packaging Material, Sheet24A-A-59136CR-971028FED Cushioning Material, Packaging, Closed Cell Foam Plank25A-A-59178DD A041012Notice 1USGOVT Nipple, Electrical Terminal26A-A-59503DC C110303FED Commercial Item Description Nitrogen, Technical27A-A-59551CP A091022USGOVT Wire, Electrical, Copper (Uninsulated)28A-A-59569DJ C090122Notice 1DLA Qualification Sampling and Testing of Steels for Transverse TensileProperties29A-A-59588DJ B120130Notice 1FED Commercial Item Description Rubber, Silicone30A-A-59877CT-100909FED Commercial Item Description Insulating Compound, Electrical, Embedding31AIR4127CG - 071101SAE Steel: Chemical Composition and Hardenability32AISI-1010Unavailable-AISI Low Carb Stl Unavailable 33AISI-50100Unavailable-AISI Bearing Stl Unavailable 34AISI-52100Unavailable-AISI Bearing Stl Unavailable 35AISI-B-1112Unavailable-AISI Low Carb Free Mach Stl Unavailable 36AISI-C-1212Unavailable-AISI Material Spec, Stl Unavailable 37AISI-C-1213Unavailable-AISI Low Carb Free Mach Stl Unavailable 38AISI-C-1214Unavailable-AISI Material Spec, Stl Unavailable 39AMS 2175CR A100601SAE Castings, Classification and Inspection of40AMS 2201Cancelled CN Can940901SAE Tolerances Aluminum and Aluminum Alloy Bar, Rod, Wire, and ForgingStock Rolled or Cold-FinishedANSI H35.2 41AMS 2221G060201SAE Tolerances, Copper and Copper Alloy Bars and Rods42AMS 2222BG J060201SAE Tolerances, Copper and Copper Alloy Sheet, Strip, and Plate43AMS 2223BF H060201SAE Tolerances Copper and Copper Alloy Seamless Tubing44AMS 2224G060201SAE Tolerances Copper and Copper Alloy Wire45AMS 2241CN R070701SAE Tolerances, Corrosion and Heat-Resistant Steel, Iron Alloy, Titanium, andTitanium Alloy Bars and Wire46AMS 2242CC G080604SAE Tolerances Corrosion and Heat Resistant Steel, Iron Alloy, Titanium andTitanium Alloy Sheet, Strip and Plate47AMS 2243DH K130601SAE Tolerances Corrosion and Heat-Resistant Steel Tubing48AMS 2248DB G110301SAE Chemical Check Analysis Limits Corrosion and Heat-Resistant Steels andAlloys, Maraging and other Highly-Alloyed Steels, and Iron Alloys49AMS 2249CN G090701SAE Chemical Check Analysis Limits Titanium and Titanium Alloys50AMS 2259CN E071201SAE Chemical Check Analysis Limits Wrought Low-Alloy and Carbon Steels51AMS 2269CN F060501SAE Chemical Check Analysis Limits Nickel, Nickel Alloys, and Cobalt Alloys52AMS 2300CU L100801SAE Steel Cleanliness, Premium Aircraft-Quality Magnetic Particle InspectionProcedure53AMS 2301CT K100801SAE Steel Cleanliness, Aircraft Quality Magnetic Particle Inspection Procedure54AMS 2303CT F100801SAE Steel Cleanliness, Aircraft Quality, Martensitic Corrosion Resistant SteelsMagnetic Particle Inspection Procedure55AMS 2304CV B100801SAE Steel Cleanliness, Special Aircraft-Quality Magnetic Particle InspectionProcedure56AMS 2310BE F060201SAE Qualification Sampling and Testing of Steels for Transverse TensileProperties57AMS 2315DF G130201SAE Determination of Delta Ferrite Content58AMS 2350Cancelled - no s/s spec CN BA891001SAE Standards and Test Methods Ok to use cancspec.59AMS 2355DB K110301SAE Quality Assurance Sampling and Testing Aluminum Alloys and MagnesiumAlloy Wrought Products (Except Forging Stock), and Rolled, Forged, orFlash Welding Rings60AMS 2360CN D070701SAE Room Temperature Tensile Properties of Castings61AMS 2370DB K110601SAE Quality Assurance Sampling and Testing Carbon and Low-Alloy SteelWrought Products and Forging Stock62AMS 2371DB J110601SAE Quality Assurance Sampling and Testing Corrosion and Heat-ResistantSteels and Alloys Wrought Products and Forging Stock63APPROVED PER LR242820AMS 2372DP G140601SAE Quality Assurance Sampling and Testing Carbon and Low-Alloy SteelForgings64AMS 2375CN D070601SAE Control of Forgings Requiring First Article Approval65AMS 2380CN F080601SAE Approval and Control of Premium-Quality Titanium Alloys66AMS 2400DG X130201SAE Plating, Cadmium67AMS 2401DF J130201SAE Plating, Cadmium Low Hydrogen Content Deposit68AMS 2403BM L041001SAE Plating, Nickel General Purpose69APPROVED PER LR242392AMS 2404DP G131201SAE Plating, Electroless Nickel70AMS 2405DL E131001SAE Electroless Nickel Plate, Low Phosphorous71AMS 2406DE M121101SAE Plating, Chromium Hard Deposit72AMS 2408DL K130901SAE Plating, Tin73AMS 2410CR K100401SAE Plating, Silver Nickel Strike, High Bake74AMS 2411DM H131201SAE Plating, Silver for High Temperature Applications75AMS 2412CN J091201SAE Plating, Silver Copper Strike, Low Bake76AMS 2416CU L101201SAE Plating, Nickel-Cadmium Diffused77AMS 2417DL J130901SAE Plating, Zinc-Nickel Alloy78AMS 2418CU H110201SAE Plating, Copper79AMS 2419BM C030501SAE Plating, Cadmium-Titanium80AMS 2420D021201SAE Plating of Aluminum for Solderability Zinc Immersion Pre-TreatmentProcess81AMS 2423See Special Notes CE D020401SAE Plating, Nickel Hard Deposit82AMS 2424CR F100401SAE NI Plate, Low Stressed Deposit83AMS 2426DL E130901SAE Coating, Cadmium Vacuum Deposition84AMS 2427DD D070701SAE Aluminum Coating Ion Vapor Deposition85AMS 2429CY D111001SAE Bronze Plate Masking86AMS 2430DB S120701SAE Shot Peening, Automatic87AMS 2432DF D130201SAE Shot Peening, Computer Monitored88AMS 2433C041001SAE Plating, Nickel-Thallium-Boron or Nickel-Boron89AMS 2434CY D110901SAE Plating, Tin-Zinc Alloy90AMS 2435Noncurrent CN G070601SAE Coating, Tungsten Carbide-Cobalt Coating, Detonation Process91AMS 2437BN C710111SAE Coating, Plasma Spray Deposition92AMS 2438CL D090701SAE Plating, Chromium Thin, Hard, Dense Deposit93AMS 2444BM A001201SAE Coating, Titanium Nitride Physical Vapor Deposition94AMS 2451CW C110701SAE Plating, Brush General Requirements95AMS 2460See Special Notes DF A130301SAE Plating, Chromium96AMS 2468Cancelled CN G981001SAE Hard Anodic Coating Treatment of Aluminum Alloys AMS 2469 97AMS 2469DM J140201SAE Hard Anodic Coating Treatment of Aluminum and Aluminum AlloysProcessing and Performance Requirements98AMS 2470DH N130701SAE Anodic Treatment of Aluminum Alloys Chromic Acid Process99AMS 2471DM H140201SAE Anodic Treatment of Aluminum Alloys Sulfuric Acid Process, UndyedCoating100AMS 2472DD F070801SAE Anodic Treatment of Aluminum Alloys Sulfuric Acid Process, Dyed Coating101AMS 2473DK H130801SAE Chemical Film Treatment for Aluminum Alloys General Purpose Coating102AMS 2474Noncurrent DD D060201SAE Chemical Treatment for Aluminum Alloys Low Electrical ResistanceAMS 2477Coating103AMS 2477DD A100401SAE Conversion Coating for Aluminum Alloys Low Electrical Coating104AMS 2481CP J100201SAE Phosphate Treatment Antichafing105AMS 2482CN D100101SAE Hard Anodic Coating on Aluminum Alloys Polytetrafluoroethylene (PTFE)-Impregnated or Codeposited106AMS 2485BY K080101SAE Coating, Black Oxide107AMS 2486CR E100501SAE Conversion Coating of Titanium Alloys Fluoride-Phosphate Type108AMS 2487CN A000301SAE Anodic Treatment of Titanium and Titanium Alloys Solution pH 12.4Maximum109AMS 2488D000606SAE Anodic Tr: Ti, Ti Alys110AMS 2515DE G130101SAE Polytetrafluoroethylene (PTFE) Resin Coating Low Build, 370 to 400 °C(698 to 752 °F) Fusion111AMS 2516DF E130301SAE Polytetrafluoroethylene (PTFE) Resin Coating High Build, 370 to 400 °C(698 to 752 °F) Fusion112AMS 2525DJ D130701SAE Graphite Coating, Thin Lubricating Film Impingement Applied113AMS 2526DE D130101SAE Molybdenum Disulfide Coating, Thin Lubricating Film Impingement Applied114AMS 2590DD-101201SAE Rotary Flap Peening of Metal Parts115AMS 2615BM F060901SAE Pressure Testing Hydraulic Pressure as Specified116AMS 2630CR C100101SAE Inspection, Ultrasonic Product Over 0.5 Inch (12.7 mm) Thick117AMS 2631CW D110701SAE Ultrasonic Inspection Titanium and Titanium Alloy Bar and Billet118AMS 2632BN A950301SAE Inspection, Ultrasonic, of Thin Materials 0.50 Inch (12.7 mm) and Under inCross-Sectional Thickness119AMS 2635Cancelled Can810701SAE Radiographic Insp ASTM E1742 120AMS 2640Cancelled CH Can960401SAE Magnetic Particle Inspection ASTM E1444 121AMS 2645Cancelled CH Can950201SAE Fluorescent Penetrant Inspection ASTM E1417 122AMS 2649DM D131201SAE Etch Inspection of High Strength Steel Parts123AMS 2658CN C091001SAE Hardness and Conductivity Inspection of Wrought Aluminum Alloy Parts124AMS 2664CH F950701SAE Brazing, Silver for Use Up to 800 °F (427 °C)125AMS 2665DH H130501SAE Brazing, Silver for Use up to 400 °F (204 °C)126AMS 2666Cancelled Can840101SAE Ag Braz, High Temp AMS 2664 127AMS 2670BK J060601SAE Brazing, Copper128AMS 2671Cancelled CH Can920101SAE Copper Brazing Corrosion and Heat Resistant Steels and Alloys AMS 2670 129AMS 2672CY G120101SAE Brazing, Aluminum Torch or Furnace130AMS 2673DB E120101SAE Brazing, Aluminum and Aluminum Alloys Molten Flux (Dip)131AMS 2675DF H130201SAE Brazing, Nickel Alloy Filler Metal132AMS 2680C010601SAE Electron-Beam Welding for Fatigue Critical Applications133AMS 2681B000301SAE Electron Beam Welding134AMS 2685Noncurrent CP E071001SAE Welding, Tungsten Arc, Inert Gas GTAW Method135AMS 2689Noncurrent CH A980201SAE Fusion Welding Titanium and Titanium Alloys136AMS 2694DE C130101SAE In-Process Welding of Castings137AMS 2700DA E111101SAE Passivation of Corrosion Resistant Steels138AMS 2728DC C120901SAE Heat Treatment of Wrought Copper Beryllium Alloy Parts139AMS 2745CJ A071201SAE Induction Hardening of Steel Parts140AMS 2750DB E120701SAE Pyrometry141AMS 2753CF C080801SAE Liquid Salt Bath Ferritic Nitrocarburizing Non-Cyanide Bath142AMS 2755Cancelled CM Can090701SAE Nitriding, Molten Salt Bath143AMS 2759CE E081001SAE Heat Treatment of Steel Parts General Requirements144AMS 2759/1CJ E090201SAE Heat Treatment of Carbon and Low-Alloy Steel Parts Minimum TensileStrength Below 220 ksi (1517 MPa)145AMS 2759/2CR F100501SAE Heat Treatment of Low-Alloy Steel Parts Minimum Tensile Strength 220 ksi(1517 MPa) and Higher146AMS 2759/3CE E080801SAE Heat Treatment Precipitation-Hardening Corrosion-Resistant and MaragingSteel Parts147AMS 2759/4CA C080301SAE Heat Treatment Austenitic Corrosion-Resistant Steel Parts148AMS 2759/5D040601SAE Heat Treatment Martensitic Corrosion Resistant Steel Parts149AMS 2759/6BM B051101SAE Gas Nitriding and Heat Treatment of Low - Alloy Steel Parts150AMS 2759/7CT B100501SAE Carburizing and Heat Treatment of Carburizing Grade Steel PartsSAE Ion Nitriding151AMS 2759/8CG A070601See SpecialNotes152AMS 2759/9CL D090501SAE Hydrogen Embrittlement Relief (Baking of Steel Parts)153AMS 2759/10CN A060601SAE Automated Gaseous Nitriding Controlled by Nitriding Potential154AMS 2759/11BW-050401SAE Stress Relief of Steel Parts155AMS 2762Noncurrent CP B020101SAE Carburizing Carbon and Low-Alloy Steel Parts156AMS 2768CR C100701SAE Heat Treatment of Magnesium Alloy Castings157AMS 2769DD B091201SAE Heat Treatment of Parts in a Vacuum158AMS 2770DM L140501SAE Heat Treatment of Wrought Aluminum Alloy Parts159AMS 2771DF E130201SAE Heat Treatment of Aluminum Alloy Castings160AMS 2772CW F110701SAE Heat Treatment of Aluminum Alloy Raw Materials161AMS 2774DC D121001SAE Heat Treatment Wrought Nickel Alloy and Cobalt Alloy Parts162AMS 2800CN D060801SAE Identification Finished Parts163AMS 2801B030301SAE Heat Treatment of Titanium Alloy Parts164AMS 2807CF B080201SAE Identification Carbon and Low-Alloy Steels, Corrosion and Heat-ResistantSteels and Alloys Sheet, Strip, Plate and Aircraft Tubing165AMS 3025CN C090901SAE Polyalkylene Glycol Heat Treat Quenchant166AMS 3106Cancelled Can830401SAE Primer, Adhesive, Corr Inhib AMS 3107 167AMS 3107A910401SAE Primer, Adhesive, Corr-Inhibiting168APPROVED PER LR242821AMS 3195DP G140501SAE Silicone Rubber Sponge, Closed Cell, Medium169AMS 3216G050901SAE Fluorocarbon (FKM) Rubber High-Temperature - Fluid Resistant LowCompression Set 70 to 80170AMS 3218C050901SAE Fluorocarbon (FKM) Rubber High-Temperature - Fluid Resistant LowCompression Set 85 to 95171AMS 3276CB E080301SAE Sealing Compound, Integral Fuel Tanks and General Purpose, IntermittentUse to 360 °F (182 °C)172AMS 3301DB H110601SAE Silicone Rubber, General Purpose, 40 Durometer173AMS 3305H900401SAE Silicone Rubber, Gen Purp, 75-85174AMS 3374DL D131101SAE Sealing Compound Aircraft Firewall, Silicone175APPROVED PER LR243042AMS 3384DP-130801SAE RUBBER, FLUOROCARBON ELASTOMER (FKM) 70 TO 80HARDNESS, LOW TEMPERATURE SEALING Tg -22 deg. F (-30 deg. C)FOR ELASTOMERIC SHAPES OR PARTS IN GAS TURBINE ENGINEOIL, FUEL AND HYDRAULIC SYSTEMS176AMS 3410J981001SAE Flux, Ag Braz177AMS 3411D981001SAE Flux Silver Brz, High Temp178AMS 3644BL G060901SAE Plastic: Polyimide for Molded Rod, Bar, and Tube, Plaque, and formedParts179AMS 3645CY D120101SAE Polychlorotrifluoroethylene (PCTFE), Compression Molded HeavySections, Unplasticized180AMS 3650CY D120101SAE Rods, Sheets, and Molded Shapes, Polychlorotrifluoroethylene (PCTFE)Unplasticized181AMS 3651Cancelled Can870401SAE PTFE AMS 3667 182AMS 3651Cancelled Can870401PTFE AMS 3652 183AMS 3651Cancelled Can870401PTFE AMS 3656 184AMS 3651Cancelled Can870401PTFE AMS 3660 185AMS 3652C930101SAE PTFE Film, Non-Crit Grade186AMS 3656CW H110801SAE PTFE Extrusions, Normal Strength, As Sintered187AMS 3657CW F110801SAE PTFE, Extrusions, Premium Strength, As Sintered188AMS 3658CW F110801SAE PTFE, Extrusions, Premium189AMS 3659CW F110801SAE Polytetrafluoroethylene (PTFE) Extrusions, Premium Strength, Sinteredand Stress-Relieved190AMS 3660CW E110801SAE Polytetrafluoroethylene Moldings191AMS 3666DB E120101SAE PTFE Sht, Glass Reinforced192AMS 3667CW E110801SAE Polytetrafluoroethylene Sheet, Molded General Purpose Grade, AsSintered193AMS 3668CW E110801SAE PTFE, Moldings, Premium Grade, A Sintered194AMS 3670/1B950401SAE Unfilled Polyamide-Imide, Bar195AMS 3824CN C950901SAE Cloth, Glass Finished for Resin Laminates196AMS 4001Cancelled CK Can070701SAE Aluminum Sheet and Plate 0.12Cu (1100-0) Annealed ASTM B209 197AMS 4013DM G140201SAE Aluminum Sheet, Laminated Surface Bonded198AMS 4015CN L070201SAE Aluminum Alloy, Sheet and Plate 2.5Mg - 0.25Cr (5052-0) Annealed199AMS 4016DE M130101SAE Aluminum Alloy, Sheet and Plate 2.5Mg - 0.25Cr (5052-H32) StrainHardened, Quarter Hard, and Stabilized200AMS 4017CN K041201SAE Aluminum Alloy Sheet and Plate 2.5Mg - 0.25Cr (5052-H34) Strain-Hardened, Half Hard, and Stabilized201AMS 4023Noncurrent CN E840401SAE Aluminum Alloy Sheet and Plate Alclad 1.0Mg - 0.60Si - 0.28Cu - 0.20Cr(Alclad 6061; -T6 Sheet, -T651 Plate)202AMS 4025CE L080701SAE Aluminum Alloy, Sheet and Plate 1.0Mg - 0.60Si-0.28Cu-0.20Cr(6061-0)Annealed203AMS 4026CE M080701SAE Aluminum Alloy, Sheet and Plate 1.0Mg -0.60Si-0.28Cu-0.20Cr (6061;-T4Sheet, T-451 Plate) Solution Heat Treated and Naturally Aged204AMS 4027CE N080701SAE Aluminum Alloy, Sheet and Plate 1.0Mg -0.60Si-0.28Cu-0.20Cr (6061;-T6Sheet, T-651 Plate) Solution and Precipitation Heat Treat205AMS 4037CY P111201SAE Aluminum Alloy, Sheet and Plate 4.4Cu - 1.5Mg - 0.60Mn (2024; - T3 FlatSheet, T351 Plate) Solution Heat Treated206AMS 4048CW N100801SAE Aluminum Alloy Sheet and Plate, Alclad 5.6Zn - 2.5Mg - 1.6Cu - 0.23Cr(Alclad 7075-O) Annealed or When Specified, "As fabricated" (Alclad 7075-F)207AMS 4049CW L101201SAE Aluminum Alloy Sheet and Plate, Alclad 5.6Zn - 2.5Mg - 1.6Cu - 0.23Cr(Alclad 7075; -T6 Sheet - T651 Plate) Solution and Precipitation HeatTreated208AMS 4056DB G101001SAE Aluminum Alloy, Sheet and Plate 4.4Mg - 0.70Mn - 0.15Cr (5083-01)209AMS 4080CN N091201SAE Aluminum Alloy, Drawn Seamless Tubing 1.0Mg - 0.60Si - 0.28Cu - 0.20Cr(6061-O) Annealed210AMS 4081CC J080601SAE Aluminum Alloy Tubing, Hydraulic, Seamless, Drawn, Round 1.0Mg -0.60Si - 0.28Cu - 0.20Cr (6061-T4) Solution Heat Treated and NaturallyAged211AMS 4083DE L121101SAE Aluminum Alloy Tubing, Hydraulic, Seamless, Drawn, Round 1.0Mg -0.60Si - 0.28Cu - 0.20Cr- (6061-T6) Solution and Precipitation HeatTreated212AMS 4086BL N060901SAE Aluminum Alloy, Drawn, Round, Seamless Hydraulic Tubing 4.4Cu-1.5Mg-0.60Mn (2024-T3) Solution Heat Treated, Cold Worked, and NaturallyAged213AMS 4088BT K070301SAE Aluminum Alloy, Drawn, Seamless Tubing 4.4Cu-1.5Mg-0.60Mn (2024-T3)Solution Heat Treated and Cold Worked214AMS 4107F051101SAE Alum Aly Die Forg, (7050-T14)215AMS 4113CH E030701SAE Aluminum Alloy, Extruded Profiles 1.0Mg - 0.60Si - 0.28Cu - 0.20Cr (6061-T6) Solution and Precipitation Heat Treated216AMS 4115CU H090701SAE Aluminum Alloy, Rolled or Cold-Finished, Bars, Rods, Wire, and FlashWelded Rings Annealed 1.0Mg - 0.60Si - 0.2Cu - 0.20Cr (6061-0)217AMS 4116CN H090701SAE Aluminum Alloy, Bars, Rods, and Wire 1.0Mg - 0.60Si - 0.3Cu - 0.20Cr(6061-T4) Cold Finished, Solution Heat Treated and Naturally Aged218AMS 4117CM J090701SAE Aluminum Alloy, Rolled or Cold Finished Bars, Rods, and Wire and FlashWelded Rings 1.0Mg -0.60Si - 0.28Cu - 0.20Cr (6061; - T6, -T651)Solution and Precipitation Heat Treated219AMS 4119Cancelled CN Can900101SAE Aluminum Alloy Bars, Rolled, Drawn, or Cold Finished 4.4Cu - 1.5Mg -AMS 41200.60Mn (2024-T351) Stress Relief Stretched220AMS 4120R020901SAE Aluminum Alloy, Rolled or Cold Finished Bars, Rods, and Wire 4.4Cu - 1.5Mg - 0.60Mn (2024) Solution Heat Treated and Naturally Aged (T4)Solution Heat Treated, Cold Worked, and Naturally Aged (T351)221AMS 4121CA H071101SAE Aluminum Alloy Bars, Rods, and Wire, Rolled or Cold Finished 4.5Cu -0.85Si - 0.80Mn - 0.50Mg (2014-T6) Solution and Precipitation HeatTreated222AMS 4123CN H060101SAE Aluminum Alloy, Rolled or Cold Finished Bars and Rods (7075-T651)Solution and Precipitation Heat Treated223AMS 4124DG E120901SAE Aluminum Alloy, Rolled or Cold Finished Bars, Rods, and Wire 5.6Zn-2.5Mg-1.6Cu-0.23Cr (7075-T7351) Solution Heat Treated, Stress Relievedby Stretching and Overaged224AMS 4128CN D071001SAE Aluminum Alloy Bars, Rolled or Cold Finished 1.0Mg - 0.60Si - 0.30Cu -0.20Cr (6061-T451) Solution Heat Treated and Stress Relieved byStretching225AMS 4132DF G130201SAE Aluminum Alloy, Die and Hand Forgings, Rolled Rings, and Forging Stock2.3Cu-1.6Mg-1.1Fe-1.0Ni-0.18Si-0.07Ti (2618-T61) Solution andPrecipitation Heat Treated226AMS 4133CN E090301SAE Aluminum Alloy Forgings and Rolled Rings 4.4Cu -0.85Si -0.80Mn -0.50Mg (2014-T6) Solution and Precipitation Heat Treated227AMS 4135Cancelled CN Can860401SAE Aluminum Alloy Forgings 4.5Cu - 0.85Si - 0.80Mn - 0.50Mg (2014-T6)AMS 4133Solution and Precipitation Heat Treated228AMS 4141CE F081001SAE Aluminum Alloy Die Forgings 5.6Zn - 2.5Mg - 1.6Cu - 0.23Cr (7075-T73)Solution and Precipitation Heat Treated229AMS 4144BN F060501SAE Aluminum Alloy, Hand Forgings and Rolled Rings 6.3Cu - 0.30Mn - 0.18Zr -0.10V - 0.06Ti (2219-T852/T851) Solution Heat Treated, MechanicallyStress Relieved, and Precipitation Heat-Treated230AMS 4149D020901SAE Aluminum Alloy, Die and Hand Forgings 5.6n - 2.5Mg - 1.6Cu - 0.23Cr(7175-T74) Solution and Precipitation Heat Treated231AMS 4150DG M130401SAE Aluminum Alloy, Extrusions and Rings 1.0Mg - 0.60Si - 0.28Cu - 0.20Cr -(6061-T6) Solution and Precipitation Heat Treated232AMS 4162D030701SAE Aluminum Alloy, Extrusions 6.3Cu - 0.30Mn - 0.18Zr - 0.10V - 0.06Ti (2219-T8511) Solution Treated, Stress Relief Stretched, Straightened, andPrecipitation Heat Treated233AMS 4173DG F130401SAE Aluminum Alloy, Extrusions 1.0Mg - 0.60Si - 0.30Cu - 0.20Cr (6061-T6511) Solution Heat Treated, Stress Relieved by Stretching,Straightened, and Precipitation Heat Treated234AMS 4181C030401SAE Aluminum Alloy, Welding Wire 7.0Si - 0.38Mg - 0.10Ti (4008) (UNSA94008)235AMS 4182CN G091201SAE Alum Aly Wire, Annealed 5.0Mg - 0.12Mn - 0.12Cr (5056-0) Annealed236AMS 4185DB E120201SAE Fill Mtl, Alum Braz, 12SI, (4047)237AMS 4188Cancelled Can861001SAE Wldg Wire AMS 4181 238AMS 4188Cancelled Can861001SAE Wldg Wire AMS 4233 239AMS 4188Cancelled Can861001SAE Wldg Wire AMS 4244 240AMS 4188Cancelled Can861001SAE Wldg Wire AMS 4245 241AMS 4188Cancelled Can861001SAE Wldg Wire AMS 4246 242AMS 4210CN K050301SAE Aluminum Alloy, Castings 5.0Si - 1.2Cu - 0.50Mg (355.0-T51) PrecipitationHeat Treated243AMS 4212CU K110201SAE Aluminum Alloy Castings 5.0Si - 1.2Cu - 0.50Mg (355.0-T6) Solution andPrecipitation Heat Treated244AMS 4214CN J080601SAE Castings, Aluminum Alloy Sand 5.0Si - 1.2Cu - 0.50Mg (355.0 T71)Solution Heat Treated and Overaged245AMS 4215CN H080301SAE Aluminum Alloy, Castings 5.0Si - 1.2Cu - 0.50Mg (C355.0-T6) Solution andPrecipitation Heat Treated246AMS 4217CN H070401SAE Aluminum Alloy, Castings 7.0Si - 0.32Mg (A356.0-T6) (formerly T6PTemper) Solution and Precipitation Heat Treated247AMS 4218CN J100101SAE Aluminum Alloy Castings 7.0Si-0.35Mg (A356.0-T6) (formerly T6PTemper) Solution and Precipitation Heat Treated248AMS 4223CN D070401SAE Aluminum Alloy, Castings 4.5Cu - 0.70Ag - 0.30Mn - 0.25Mg - 0.25Ti(A201.0-T4) Solution Heat Treated and Naturally Aged249AMS 4224Cancelled - no s/s spec CN C100101SAE Aluminum Alloy Castings, Sand 4.0Cu - 2.1Ni - 2.0Mg - 0.30Cr - 0.30Mn -0.13T - 0.13V (243.0) Stabilized Ok to use canc spec.250AMS 4225CN D070601SAE Aluminum Alloy, Heat Resistant, Castings 5.0Cu - 1.5Ni - 0.25Mn - 0.25Sb- 0.25Co - 0.20Ti - 0.20Zr (203.0-T6) Solution Heat Treated andPrecipitation Heat Treated251AMS 4226Noncurrent CN A830101SAE Aluminum Alloy Castings, High Strength 5.0Cu - 0.35Mn - 0.18Zr- 0.10V(224.0) Solution and Precipitation Heat Treated (Overaged)252AMS 4227Cancelled - no s/s spec CN E050701SAE Aluminum Alloy, Casting, Sand, 8.0Cu 6.0Mg 0.50Mn 0.50Ni, As Cast Ok to use cancspec.253AMS 4229DA F120201SAE Aluminum Alloy Castings, High Strength 4.5Cu - 0.7Ag - 0.30Mn - 0.25Mg -0.25Ti (A201.0-T7) Solution Heat Treated and Overaged254AMS 4233C030301SAE Aluminum Alloy, Welding Wire 4.5 Cu - 0.70Ag - 0.30Mn - 0.25Mg - 0.25Ti(A201.0-T7) Solution Heat Treated and Overaged255AMS 4235CN B080301SAE Aluminum Alloy Castings 4.6Cu - 0.35Mn - 0.25Mg - 0.22Ti (A206.0-T71)Solution and Precipitation Heat Treated256AMS 4236DF C130201SAE Aluminum Alloy Castings 4.6Cu - 0.35Mn - 0.25Mg - 0.22Ti (A206.0-T4)Solution Heat Treated and Naturally Aged257AMS 4237Cancelled - no s/s spec CN B070401SAE Aluminum Alloy Castings, Sand 4.6Cu - 0.35Mn - 0.25Mg - 0.22Ti (206.0 -T71) Solution Heat Treated and Naturally Aged Ok to use canc spec.258AMS 4241CN D091101SAE Aluminum Alloy Castings 7.0Si - 0.58Mg - 0.15Ti -0.06Be (D357.0 - T6)Solution and Precipitation Heat Treated Dendrite Arm Spacing (DAS)Controlled259AMS 4244CE B080701SAE Aluminum Alloy, Welding Wire 4.6Cu - 0.35Mn - 0.25Mg - 0.22Ti forWelding A206.0 Type Alloys260AMS 4245CR E100401SAE Aluminum Alloy, Welding Wire 5.0Si - 1.2Cu - 0.50Mg (355) (UNS A03550)261AMS 4246Noncurrent CP D080201SAE Aluminum Alloy, Welding Wire 7.0Si - 0.52Mg (357) (UNS A03570)262AMS 4260Not Acceptable to Use atParker HannifinAerospaceCL G080601SAE Alum Aly Cast, Invest (356.0-T6)BPS4829263AMS 4261CN F091201SAE Aluminum Alloy Castings, Investment 7.0Si - 0.32Mg (356.0 - T51)Precipitation Heat Treated264AMS 4280CN J080601SAE Aluminum Alloy Castings, Permanent Mold 5.0Si - 1.2Cu - 0.5Mg (355.0-T71) Solution Heat Treated and Overaged265AMS 4284DC J110301SAE Aluminum Alloy Castings, Permanent Mold 7.0Si - 0.30Mg (356.0-T6)Solution and Precipitation Heat Treated266AMS 4289CN-011101SAE Aluminum Alloy Castings 7.0Si - 0.55Mg - 0.12Ti (F357.0-T6) Solution andPrecipitation Heat Treated267AMS 4291CT H101001SAE Aluminum Alloy, Die Castings 8.5Si - 3.5Cu (A380.0-F) (See AS1990) AsCast268AMS 4315CK-050701SAE Aluminum Alloy Sheet and Plate 5.6Zn - 2.5Mg - 1.6Cu - 0.23Cr 7075: (-T76 Sheet, -T7651 Plate) Solution and Precipitation Heat Treated269AMS 4316CY A111101SAE Aluminum Alloy, Alclad Sheet and Plate 5.6Zn - 2.5Mg270AMS 4437DM F140201SAE Magnesium Alloy Castings, Sand 8.7Al - 0.70Zn (AZ91C-T6) Solution HeatTreated and Aged271AMS 4507BW H011101SAE Copper Alloy (Brass), Sheet, Strip, and Plate 70Cu - 30Zn Half Hard (H02) 272AMS 4510CN G010501SAE Phosphor Bronze, Sheet, Strip, and Plate 94.5Cu - 4.0Sn - 0.19P SpringTemper (H08)273AMS 4511A040701SAE Copper Beryllium Alloy Castings 97Cu-2.1Be-0.52(Co+Ni)-0.28Si Solutionand Precipitation Heat Treated (TFOO)274AMS 4530CY J110901SAE Copper-Beryllium Alloy Sheet, Strip, and Plate 98Cu - 1.9Be Solution HeatTreated (TB00)275AMS 4533DF D130201SAE Copper-Beryllium Alloy, Bars and Rods 98Cu - 1.9Be Solution andPrecipitation Heat Treated (TF00, formerly AT)-UNS C17200276AMS 4597CY A111201SAE Copper-Nickel-Tin Alloy, Bars and Rods 77Cu - 15Ni - 8Sn SolutionAnnealed, Cold Finished and Spinodal Hardened (TX TS)277AMS 4631Noncurrent CL E880401SAE Aluminum Bronze Rods, Bars, and Forgings 90.5Cu - 7.5Al - 1.95: StressRelieved278AMS 4633CL A031201SAE Bronze, Aluminum Silicon, Rods, Bars, and Forgings 90Cu - 7.0Al - 1.8SiDrawn and Stress Relieved (HR50)279AMS 4634CL B090301SAE Aluminum Bronze Bars, Rods, and Forgings 905Cu - 7.5Al - 1.9Si StressRelieved280AMS 4635CL F090701SAE Aluminum Bronze Bars, Rods, and Forgings 87Cu - 9Al - 3Fe StressRelieved281AMS 4640CV H110501SAE Aluminum Bronze, Bars, Rods, Shapes, Tubes, and Forgings 81.5Cu -10.0Al - 4.8Ni - 3.0Fe Drawn and Stress Relieved (HR50) or TemperAnnealed (TQ50)282AMS 4650DF M130201SAE Copper-Beryllium Alloy, Bars, Rods, Shapes and Forgings 98Cu - 1.9BeSolution Heat Treated TB00 (A)283AMS 4651CN C050701SAE Copper-Beryllium Alloy, Bars and Rods 98Cu - 1.9Be (CDA 172) HardTemper (TD04)284AMS 4674CN G060901SAE Nickel - Copper Alloy, Corrosion-Resistant, Bars and Forgings 67Ni - 30Cu- 0.04S Free Machining285AMS 4701CN G091001SAE Copper Wire, Oxygen-Free 99.95 (Cu+Ag) Annealed286AMS 4730CN G080701SAE Nickel-Copper Alloy Wire, Corrosion-Resistant 67Ni-31Cu Annealed (400) 287AMS 4765E990501SAE Braz Fill Mtl288AMS 4769F990501SAE Braz Fill Mtl289AMS 4770K990501SAE Braz Fill Mtl290AMS 4772J990501SAE Braz Fill Mtl291AMS 4774F990501SAE Braz Fill Mtl292AMS 4775CT J101001SAE Nickel Alloy, Brazing Filler Metal 73Ni - 0.75C - 4.5Si - 14Cr - 3.1B - 4.5Fe1790 to 1970 °F (977 to 1077 °C) Solidus-Liquidus Range293AMS 4776CT H101001SAE Nickel Alloy, Brazing Filler Metal 73Ni - 4.5Si - 14Cr - 3.1B - 4.5Fe (LowCarbon) 1790 to 1970 °F (977 to 1077 °C) Solidus-Liquidus Range294AMS 4777CT H101001SAE Nickel Alloy, Brazing Filler Metal 82Ni - 4.5Si - 7.0Cr - 3.1B - 3.0Fe 1780 to1830 °F (971 to 999 °C) Solidus-Liquidus Range295AMS 4786CN H090701SAE Gold-Palladium-Nickel Alloy, Brazing Filler Metal, High Temperature 70 Au- 8.0Pd - 22Ni 1845 to 1915 °F (1007 to 1046 °C) Solidus-Liquidus Range 296AMS 4787F000401SAE Gold-Nickel Alloy, Brazing Filler Metal, High Temperature 70Au - 8.0Pd -22Ni 1845 to 1915。

试剂盒使用说明和包被实验示例：一、包被液的配制：coating buffer 包被缓冲液（EL-CPX01）：该试剂为10X 浓缩液，使用前请根据实验用量，用蒸馏水稀释至1X，即配即用，例：10ml coating buffer + 90ml蒸馏水；Coating buffer 用于抗体的包被；可替代以PBS为主的包被液体；请用1* coating buffer 和适量一抗配成包被液，抗体浓度请参考抗体的说明。

二、包被实验示例：在ELISA酶标板（EL-CPX02）中每孔加入100ul包被液（包被液若有剩余可多包被一点，以条为单位），在板上贴膜（封板，plate sealer，），室温（控制室温在25℃，板放置处旁放置温度计控温）温育过夜（下午4点到第二天早上9点，不宜缩短时间，会降低包板效率）；结束温育（也请参考抗体说明书的包被条件进行相应调整）。

多余的配好的包被液不能保存。

三、洗板封闭：1)将洗液20×Wash Buffer（EL-00402）（可以用PBS +Tween 20代替）配制成1×Wash Buffer（实验前请合理计算用量，配制成足够的量待用），用1×Wash Buffer洗板4次（注：包被组分在加稳定剂前不能剧烈震荡或完全干燥，所以洗板过程需快速、温和，不要拼命拍板）；2)洗板后立即加入200ul每孔的酶标板稳定剂（EL-CPX03），37℃温育1小时；3)弃掉或吸取酶标板稳定剂（注：拍干，千万不要洗板）。

封闭好的酶标板可以有以下几种处理方式。

方式一：直接进行检测。

方式二：冻干（需有冻干机，长期保存）1)冻干成品：把酶标板放入冰箱，-20℃冰箱冷冻过夜。

2)调试真空冻干机，立即将冷冻过夜的酶标板放入真空冻干机冻干24小时；3)取出冻干的酶标板，立即将酶标板放入一个装有干燥剂的铝箔袋内（EL-CPI05-20），贴上标签，放4℃冰箱长期保存。

（冻干同时可进行标准品冻干，标准品冻干需要购买标准品冻干液）。

REF 10213-4 4 x 24mL总蛋白 (TP)楔形瓶，每瓶含试剂可用量 24mL。

预期用途EasyRA® TP 试剂目的是用于通过 MEDICA EasyRA 临床化学分析仪进行人血清或血浆（采用肝素锂作为抗凝血剂）中总蛋白的定量测定。

仅用于体外诊断用途。

仅供专业人员使用。

摘要和说明1, 2, 3在人血清中，血清蛋白主要包括白蛋白（占总蛋白的50-60%），其余部分包括α1-、α2-、β- 和γ-球蛋白。

总蛋白的浓度对维持血液和组织之间水的正常平衡与交换是很重要的。

血清蛋白浓度较低，可能是由于吸收不良、合成受损引起的，或是由于出血或过度分解代谢导致蛋白损失而引起的，如肾病和肝病中观察到的情况，这种情况可能导致低蛋白血症。

在高免疫球蛋白血症（如多发性骨髓瘤和感染）或脱水情况下，可能发生高蛋白血症。

方法的原理本分析法采用双缩脲反应测定血清蛋白，其中碱性溶液中的二价铜离子与化合物（含 2 个或多个、与碳原子结合的酰胺基或肽基）反应，形成一种有色络合物4。

碱性 pH血清蛋白质类 + Cu++———— 紫色络合物紫色络合物以分光光度法在 550nm 处测定，以 700nm 作为空白波长。

在较宽的线性范围内，该络合物的密度与蛋白质浓度成正比5。

试剂五水合硫酸铜0.3g/dL氢氧化钠0.8g/dL碘化钾、酒石酸钾钠和叠氮化钠作为防腐剂。

注意事项1.当处理任何实验室试剂时，应遵守良好实验室安全规范(CLSI, GP17-A2)。

2.氢氧化钠具有腐蚀性，能引起烧伤。

不要用嘴吸取试剂。

如果吞服，应立即就医处理。

避免眼部和皮肤接触。

如发生眼部接触，立即用大量水清洗眼睛，并就医处理。

如果发生皮肤接触，用水清洗皮肤至少 15 分钟。

3.本试剂含叠氮化钠 <0.1%；叠氮化钠可与铅和铜水管反应，形成高爆炸性金属叠氮化物。

请参考化学品安全说明书中的危险、危害和安全信息。

4.就任何诊断试验方法而言，其结果应根据所有其它试验结果和患者的临床状况加以解释。

Schillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************Certificate We hereby certify, that the below mentioned products meetGMP-guidelines, EG –GMP-Annex 15 guidelineand PIC/S guideline PI 006-3 requirements.Products: SWB805 MultiMount™SLB815 Load CellAJB941M Junction BoxWeigh Module Mounting KitsManufacturer: Mettler-Toledo(Changzhou)Precision Instrument Ltd.No. 22 Zhengqiang Road,Changzhou, Jiangsu, P.R.ChinaGau-Algesheim, March 2018Prof. Ronald Ziegler M. Sc.Roland Pichler, Dipl.-Ing.(FH) PharmatechnikSchillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************Index1.Description of the products (3)1.1SWB805 MultiMount™ (3)1.2SLB815 Load Cell (3)1.3AJB941M Junction Box (4)1.4Weigh Module Mounting Kits (5)2. General requirements for Industrial Scales (6)3. Appraisal criteria for an optimal cleaning (7)4. Appraisal factors for qualification (10)Schillerstraße 65 71229 Leonberg www.ig-pharma.deSubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070 E-Mail:*****************1. Description of the products1.1 SWB805 MultiMount™The SWB805 is a fully approved hygienic weigh module for food and pharmaceutical applications.Model no.:SWB805 MultiMountMaterial:304 stainless steel / 316 stainless steel HD-9570 Silicone RubberFinish:Electro polish, final mirror polish, RA < 0.8µm Hygienic approval: NSF1.2 SLB815 Load CellLoad Cell for SWB805 MultiMount Weigh ModuleModel no.:SLB815Rated capacity (R.C.) [kg (lb, nominal)]:110 (250), 220 (500), 550 (1,250), 1100 (2,500), 2,200 (5,000), 4,400 (10,000)Temperature range [°C (°F)] Compensated: -10 ~ +40 (+14 ~ +104) Operating: -40 ~ +65 (-40 ~ +150) Safe storage: -40 ~ +80 (-40 ~ +176Hygienic approval Hygienic approval:NSFSchillerstraße 65 71229 Leonberg www.ig-pharma.deSubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070 E-Mail:*****************Excitation voltage [V AC/DC] Recommended: 5 ~ 15 Max.:15Terminal resistance Ω Excitation: 384 ± 10 Output: 350 ± 1 Material Spring element: 17-4 PH stainless steelFinish: Electro polish, final mirror polish, RA < 0.8µmProtection Type: welded IP rating: IP68, IP69K NEMA rating: NEMA 6/6PCableDiameter [mm (in)]:PVC: 5.2 (0.2)1.3 AJB941M Junction BoxPrecision Junction Boxes are intended to connect analog load cells and allow very precise shift adjustment by selecting precision resistors via two hex-step switches.P/N: 30219978Size: Medium Application: Hygienic No. of load cells:2, 3, 4Temperature range [°C (°F)] Operating: -30 ~ +65 (-4 ~ +150) Safe storage:-40 ~ +80 (-40 ~ +176)Schillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************MaterialEnclosure: 316 stainless steel, FDA approvedCable entry fitting: stainless steelFinish: Electro polish, final mirror polish, RA < 0.8µmProtectionType: SiliconIP rating: IP69KHygienic approvalHygienic approval:NSF1.4 Weigh Module Mounting KitsWelding and bolting kits are available which meet hygienic requirements. These kits con-tain hygienic fasteners and seals for all metal to metal interfaces. The top and base plates may be secured using Welding Kits, Bolt Kits, or Concrete Kits. Any combination of kits can be used for the top and bottom mounting.Schillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************2. General requirements for Industrial ScalesIndustrial Scales (weigh modules) have to show the suitable measurement range and the re-quired precision (EC-GMP guide1, chapter 3.40).They have to be calibrated regularly, which has to be documented (EC-GMP guide, chap-ter 3.41).The permitted tolerance must be provided for the respective weighing capacity, by considera-tion of the measuring inaccuracies, i.e. the still tolerated deviation of the debit value. Working with raw materials, the equipment and the utensils used have to meet the require-ments for surfaces in pharmaceutical production.According to § 211.65 “C onstruction of the equipment”2 of the FDA: …(a) Equipment shall be constructed so that surfaces that contact components, in-process materials, or drug products shall not be reactive, additive, or absorptive so as to alter the safety, identity, strength, qual-ity, or purity of the drug product beyond the official or other established requirements.”The cleaning ability is confirmed by the cleaning validation. Established cleaning instructions are necessary as a prerequisite for a cleaning validation.The amount of permitted residuals whether active pharmaceutical ingredients or cleaning agents, is dependent on the preliminary manufactured product. These include the derivative product and the lot size of the derivative product. A first, general statement for not critical products can be made with the criteria "visual clean". According to the literature3, backlogs of 375 µg per 100 cm² are no longer visible.1EC-GMP guide, chapter 3.402USA 21 CFR Part 2113Buscalferri et.al., Pharmind 62, Nr. 6 (2000)Schillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************3. Appraisal criteria for an optimal cleaningGeneralIn principle, those parts of equipment, which come into contact with the product, have to be cleaned well. Regarding the balances, these parts are the load plates.Both the FDA inspection guideline for cleaning validation and the PIC guideline PIC/S 006-3 cite the visual criterion as one out of three possible acceptance criterions. From these the most appropriate criterion has to be chosen to appraise the cleaning success.The products SWB805 MultiMount™, SLB815 Load Cell, AJB941M Junction Box and Weigh Module Mounting Kits were subjected to a qualified examination regarding "cleaning ability"4 This means that the surfaces of the products SWB805 MultiMount™, SLB815 Load Cell, AJB941M Junction Box and Weigh Module Mounting Kits must be free of visible residuals after cleaning. As a basis for this examination the works of Buscalferri, F., Assignation of the visibility limit of pharmaceutical active agents (“Bestimmung der Sichtbarkeitsgrenze von pharmazeutischen Wirkstoffen”), master thesis, Albstadt-Sigmaringen University, course of studies pharmaceutical technology (1999) and Fourman, G. L., Mullen, Determining Cleaning Validation Acceptance Limits for Pharmaceutical Manufacturing Operations , Pharm. Technol. 17 (4), 54 (1993) were used.4 The examinations and their evaluation were carried out by Prof. R. ZieglerSchillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************ProcedureA granulated material, which was coloured with Erythrosine, was used as a sample. The ex-aminations51 were carried out according to the “visually clean” criteria (please see “GMP Berater” 8.E.1.5). First the load plates of the products SWB805 MultiMount™,SLB815 Load Cell, AJB941M Junction Box and Weigh Module Mounting Kits were polluted, following by a cleaning step. A cleaning agent (P3-cosa PUR 80 Manufacturer: Ecolab GmbH & Co. OHG, Düsseldorf), which is commonly used in pharmaceutical production was taken and feigned according to different pollution degrees.The cleaning success was then appraised visually.The exact data can be taken from the test report. These results show clearly, that the depletion degree meets in principle the hygienic requirements. In principle the depletion degree is de-pendent on the examined material and the specific requirements of the examination.ResultsIn regard to the cleaning, the products SWB805 MultiMount™, SLB815 Load Cell,AJB941M Junction Box and the Weigh Module Mounting Kits , correspond to the visually clean criteria.5These experiments were performed by Prof. R. ZieglerSchillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************SummaryThe construction of the products SWB805 MultiMount™, SLB815 Load Cell, AJB941M Junc-tion Box and the Weigh Module Mounting Kits are GMP-compliant. The cleaning of the parts, which come in contact with the product, has to be carried out well. There are no inaccessible places where dust can accumulate. Once the load plate is installed, the load plate will be welded to the vessel, so that the cleaning of the parts which are not in contact with the prod-uct is guaranteed.The SWB805 MultiMount™, SLB815 Load Cell, AJB941M Junction Box and Weigh Module Mounting Kits are easy to clean as well. Every part is easily accessible. The mechanic com-ponents are constructed according to the GMP guidelines.Schillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 4 55435 Gau-Algesheim Fon +49 6725 9111070E-Mail:*****************4. Appraisal factors for qualificationGeneralThe PIC/S guideline PI 006-3 and the EG-GMP guide Annex 15 mention principles for qualifi-cation and validation.Every machine or equipment which directly or indirectly influences the quality of the product shall be qualified. The machine or equipment shall be designed in agreement with the pre-vailing GMP guidelines. The machine shall be installed in agreement with the design specifi-cation and the functions shall be checked with the available documentation (functional qualifi-cation).ProcedureThe available documentation of the METTLER TOLEDO products SWB805 MultiMount™, SLB815 Load Cell, AJB941M Junction Box and Weigh Module Mounting Kits was checked to the effect of whether design qualification, installation qualification and functional qualification are feasible.Schillerstraße 65 71229 Leonberg www.ig-pharma.de SubsidiaryLise-Meitner-Straße 455435 Gau-AlgesheimFon +49 6725 9111070E-Mail:*****************Page 11 of 11ResultsThe documentation of the METTLER TOLEDO products SWB805 MultiMount™,SLB815 Load Cell, AJB941M Junction Box and the Weigh Module Mounting Kits is very de-tailed. Also an exact description of the modules with design drawings is available. The mate-rials used are described precisely.GMP-relevant documents are available (e.g. inspection certificate, CE-mark). Particulars re-garding the maintenance are furnished.SummaryThe documentation of the manufacturer of the METTLER TOLEDO productsSWB805 MultiMount™, SLB815 Load Cell, AJB941M Junction Box and the Weigh Module Mounting Kits is written in a very detailed way and offers the necessary conditions for the ex-ecution of qualification, as it is demanded by EG-GMP guide Annex 15 and PIC/S guideline PI 006-3.。

WHO International Standard1st WHO International Standard for Human Papillomavirus (HPV)Type 16 DNA NIBSC code: 06/202 Instructions for use(Version 2.0, Dated 10/11/2010)1. INTENDED USEThe 1st International Standard for HPV Type 16 (HPV-16) DNA Nucleic Acid Amplification Techniques consists of a freeze-dried preparation of recombinant plasmid containing full-length HPV-16 DNA cloned via its unique BamH1 site (Quint et al., 2006). The standard has been formulated in a background of purified human genomic DNA, lyophilized in 0.5 ml aliquots and stored at -20 °C. The material was calibrated in an international collaborative study involving 19 laboratories (Wilkinson et al., 2008). The International Standard contains material that is proprietory to third parties and should be used for the sole purpose of calibrating in-house or working standards for the amplification and detection of HPV-16 DNA. The International Standard should not be used for any other purpose and should be discarded after use. 2. CAUTIONThis preparation is not for administration to humans .This material contains DNA derived from C33A cells. As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratory's safety procedures. Such safety procedures should include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts.3. UNITAGEThe 1st International Standard for HPV-16 DNA Nucleic Acid Amplification Techniques has been assigned a unitage of 5 x 106 International Units (IU) per ampoule.Traceability statement:It was proposed at a WHO meeting in January 2008 (WHO Meeting Report, 2008) that the instructions for use of the International Standard for HPV-16 DNA include the calculations and assumptions used in determining the theoretical HPV-16 qenome equivalents (GEq) of the bulk material used in formulating the International Standard, thus demonstrating that 1 IU is equivalent to 1 GEq for HPV-16 DNA . The definitive unitage of the 1st WHO International Standard for HPV-16 DNA therefore remains as IU while the traceability statement would allow users to equate IU with GEq.Assays for DNA concentration of the recombinant HPV-16 plasmid stock preparation were performed in Dr Cosette Wheeler‟s laboratory, University of New Mexico (UNM). DNA concentrations were determined by absorbance at 260 nm as well as spectrofluorometrically using the Picogreen assay (Invitrogen Corporation, USA). A correlation coefficient of 0.95 or higher was obtained between the two DNA measurements. 10 ng HPV-16 plasmid DNA/μl was supplied to NIBSC for formulating the bulk material for subsequent freeze-drying. The UNM laboratory also provided NIBSC with a statement indicating that 1.0 x 1011 GEq/ml for HPV-16 is equal to 1.17 ng/μl. 10 ng HPV-16 plasmid DNA/μl plasmid stock preparation is therefore equivalent to 8.547 x 1011 HPV-16 GEq/ml. NIBSC used this data in formulating the 1st International Standard for HPV Type 16 DNA.Formulation of bulk material for the 1st International Standard for HPV Type 16 DNA (NIBSC code 06/202):At NIBSC, the bulk HPV-16 plasmid DNA material was prepared according to the formula:HPV GEq/ml of bulk material = (HPV GEq/ml of plasmid stock x volume plasmid stock) / volume bulk material.Therefore,HPV-16 GEq/ml of bulk material = (8.547 x 1011 HPV-16 GEq/ml plasmid stock) x (0.02223 ml HPV-16 plasmid stock) / 1900 ml HPV-16 bulk material = 1.0 x 107 HPV-16 GEq/ml bulk materialThe HPV-16 DNA bulk material was subsequently freeze-dried in 0.5 ml aliquots.Certain assumptions are required for equating IU to GEq for the 1st International Standard for HPV-16 DNA: 1) 1.0 x 1011 GEq/ml for HPV-16 is equal to 1.17 ng/μl. 2) There is no loss in activity of the HPV-16 DNA upon lyophilization. 3) The recombinant HPV-16 plasmid DNA accurately mimics the activity of HPV-16 viral DNA in biological samples.Independent calculation of GEq/ml for recombinant HPV-16 plasmid DNA.NIBSC also independently calculated the genome equivalence of the HPV-16 plasmid stock preparation and bulk preparation in which the molecular weights of the full-length HPV-16 genome and pBR322 DNA were based on sequence content using BioEdit Sequence Alignment Editor v7.0.5.3 (Tom Hall, Isis Pharmaceuticals Inc., USA). The sequences used for determining the molecular weights are GenBank Accession number J01749.1 for pBR322 and the reference sequence for HPV16 (Accession K02718).BioEdit dataDNA molecule: HPV16 Accession K02718 Length = 7904 base pairsMW= 4786756.00 Daltons, double strandedDNA molecule: cloning vector pBR322 Length = 4361 base pairsMW= 2653867.00 Daltons, double strandedFormulaeGEq/ml of the HPV plasmid stock was calculated according to the formula: GEq/ml of the HPV plasmid stock = (DNA concentration of HPV plasmid stock) x (MW of HPV DNA + MW of pBR322)-1 x (Avogadro‟s Number) where Avogadro‟s Number = 6.022x1023 molecules/molGEq/ml of the bulk HPV DNA materials was calculated according to the formula:HPV GEq/ml of bulk material = (HPV GEq/ml of plasmid stock x volume plasmid stock) / volume bulk material.CalculationThe recombinant HPV-16 plasmid stock preparation was supplied to NIBSC at a concentration of 10 ng/μl. Using the MW determinations shown above, the GEq/ml of the HPV-16 plasmid stock is:= (10 x 10-9 g/μl) x (mol/(7440623 g) x (6.022x1023 molecules/mol) = 8.093 x 108 molecules/μl = 8.093 x 1011molecules/ml = 8.093 x 1011 HPV-16 GEq/ml22.23μl of the recombinant HPV-16 plasmid stock was diluted to a final volume of 1900ml, therefore,HPV-16 GEq/ml of bulk material = (8.093 x 1011 HPV-16 GEq/ml plasmid stock) x (0.02223 ml HPV-16 plasmid stock) / 1900 ml HPV-16 bulk material = 0.947 x 107 HPV-16 GEq/ml bulk material4. CONTENTSCountry of origin of biological material: United Kingdom.Each ampoule contains the lyophilized equivalent of 0.5 ml HPV-16 plasmid DNA in 10mM Tris buffer pH7.4 containing 1mM EDTA, 5 mg/ml trehalose and ~1 x 106 human GEq/ml derived from C33a cells.5. STORAGEThe ampoule should be stored at -20 °C or below on receipt.Please note: because of the inherent stability of lyophilized material, NIBSC may ship these materials at ambient temperature.6. DIRECTIONS FOR OPENINGDIN ampoules have an …easy -open‟ coloured stress point, where the narrow ampoule stem joins the wider ampoule body.Tap the ampoule gently to collect the material at the bottom (labeled) end. Ensure that the disposable ampoule safety breaker provided is pushed down on the stem of the ampoule and against the shoulder of the ampoule body. Hold the body of the ampoule in one hand and the disposable ampoule breaker covering the ampoule stem between the thumb and first finger of the other hand. Apply a bending force to open the ampoule at the coloured stress point, primarily using the hand holding the plastic collar.Care should be taken to avoid cuts and projectile glass fragments that might enter the eyes, for example, by the use of suitable gloves and an eye shield. Take care that no material is lost from the ampoule and no glass falls into the ampoule. Within the ampoule is dry nitrogen gas at slightly less than atmospheric pressure. A new disposable ampoule breaker is provided with each DIN ampoule.7. USE OF MATERIALNo attempt should be made to weigh out any portion of the freeze-dried material prior to reconstitution.The 1st International Standard for HPV-16 DNA contains high copy number template. There is a high risk of HPV-16 plasmid DNA contamination via aerosolization upon opening of the glass ampoule. The material must be opened and handled in a separate laboratory environment, away from other pre-amplification components such as reagents, labware and samples.The material is supplied lyophilized and, before use, should be reconstituted in 0.5 ml sterile nuclease-free water. Ensure that the inside surface of the ampoule is wetted with the added water so that any particles of freeze-dried material adhering to the glass are reconstituted. The reconstituted material has a final concentration of 1 X 107 IU/ml. The reconstituted material is suitable for calibration of in-house or working standards for the amplification and detection of HPV-16 DNA.. The material is not suitable for calibrating or assessing extraction, precipitation or centrifugation procedures. The material has NOT been calibrated for human DNA nucleic acid amplification techniques.8. STABILITYReference materials are held at NIBSC within assured, temperature-controlled storage facilities. The 1st International Standard for HPV-16 DNA should be stored at -20 °C or below on receipt.Studies on the stability of reconstituted standard are underway. Users should determine the stability of the reconstituted material according to their own method of preparation, storage and use.NIBSC follows the policy of WHO with respect to its reference materials.9. REFERENCESQuint, W. G. V., Pagliusi, S. R., Lelie, N., de Villiers, E. M., Wheeler, C. M. and the World Health Organization Human Papillomavirus DNA International Collaborative Study Group. (2006). Results of the First WorldHealth Organization International Collaborative Study of Detection of Human Papillomavirus DNA. J. Clin. Microbiol. 44: 571-579.Wilkinson, D.E., Baylis, S.A., Padley, D., Heath, A.B., Ferguson, M., Pagliusi, S.R., et al. Establishment of the 1st World Health Organization international standards for human papillomavirus type 16 DNA and type 18 DNA. Int J Cancer 2010 Jun 15;126(12):2969-83.WHO meeting report, on “Standardization of HPV assays and the role of HPV LabNet in supporting vaccine introduction” Geneva, Switzerland, 23-25 January 2008, in preparation.10. ACKNOWLEDGEMENTS11. FURTHER INFORMATIONFurther information can be obtained as follows; This material: enquiries@ WHO Biological Standards:http://www.who.int/biologicals/en/JCTLM Higher order reference materials: /en/committees/jc/jctlm/ Derivation of International Units:/products/biological_reference_materials/frequently _asked_questions/how_are_international_units.aspx Ordering standards from NIBSC:/products/ordering_information/frequently_asked_q uestions.aspxNIBSC Terms & Conditions:/terms_and_conditions.aspx12. CUSTOMER FEEDBACKCustomers are encouraged to provide feedback on the suitability or use of the material provided or other aspects of our service. Please send any comments to enquiries@13. CITATIONIn all publications, including data sheets, in which this material is referenced, it is important that the preparation's title, its status, the NIBSC code number, and the name and address of NIBSC are cited and cited correctly.15. LIABILITY AND LOSSInformation provided by the Institute is given after the exercise of all reasonable care and skill in its compilation, preparation and issue, but it is provided without liability to the Recipient in its application and use. It is the responsibility of the Recipient to determine the appropriateness of the standards or reference materials supplied by the Institute to the Recip ient (“the Goods”) for the proposed application and ensure that it has the necessary technical skills to determine that they are appropriate. Results obtained from the Goods are likely to be dependant on conditions of use by the Recipient and the variability of materials beyond the control of the Institute.All warranties are excluded to the fullest extent permitted by law, including without limitation that the Goods are free from infectious agents or that the supply of Goods will not infringe any rights of any third party.The Institute shall not be liable to the Recipient for any economic loss whether direct or indirect, which arise in connection with this agreement.The total liability of the Institute in connection with this agreement, whether for negligence or breach of contract or otherwise, shall in no event exceed 120% of any price paid or payable by the Recipient for the supply of the Goods.If any of the Goods supplied by the Institute should prove not to meet their specification when stored and used correctly (and provided that the Recipient has returned the Goods to the Institute together with written notification of such alleged defect within seven days of the time when the Recipient discovers or ought to have discovered the defect), the Institute shall either replace the Goods or, at its sole option, refund the handling charge provided that performance of either one of the above options shall constitute an entire discharge of the Institute‟s liability under this Condition.。

Table of ContentsLB Medium (1)NZ Medium (2)SM Buffer (3)SET Buffer (4)6X Prehyb Soln (5)10 X TBE (6)10 X TAE (7)20 X SSC (8)1% SDS, 0.2 M NaOH (9)14% PEG (8000), 2M NaCl, 10 mM MgSO4 (10)20% SDS (11)1.0 M Tris, pH 8.0, 1.5 M NaCl (12)10mM Tris-HCl, pH 7.5, 10mM MgSO4 (13)10 mM Tris, 50 mM EDTA, pH 7.5 (14)10 mM Tris-HCl, 1 mM EDTA, pH 7.5 (15)3 M Sodium Acetate, pH 4.8 (16)Electrophoresis dye (17)Labelling Stop dye (18)Sequencing gel dye (19)5% Acrylamide (20)6% Acrylamide in TBE, 50% Urea (21)40% Acrylamide (22)LB Medium (1 Liter)10g Bacto-tryptone5g Bacto-yeast extract10g NaClFor forty plates add 1% agar--1g. Autoclave media. When cool, add ampicillin and pour plates. For 1L of media, add 1.8 mL amp.NZ Medium (500 mL)5 g Bacto-tryptone2.5 g Bacto-yeast extract2.5 g NaCl1.25 g MgSO4For 20 plates add 1.2% agar--6g. Autoclave and pour plates at 50o CSM Buffer (1L)5.8 g NaCl1.2 g MgSo450 mL 1M Tris-HCl, pH 7.50.1 g Gelatin (doesn't dissolve)AutoclaveUsed for phage dilution and storage.SET Buffer50 mM Tris-HCl, pH 8.0, 50 mM EDTA, 20% w/v Sucroseto make 200mL:40 g Sucrose10 mL of 1M Tris20 mL of 0.5 M EDTA, disodium saltbring to 200 mL with H206X Prehybridization Solutionto make 500 mL300 mL ddH20150 mL 20X SSC50 mL 50X Denhardt's solution1 mL 0.5 M EDTA (disodium salt)2.5 mL 20% SDS6X refers to the concentration of SSC10X TBE Buffer (for polyacrylamide gels) to make one liter:60.75 g Tris3.7 g EDTA (tetrasodium salt)30 g Boric acid10X TAE Buffer (For agarose gels)to make one liter:48.20 g Tris6.75 g NaAce3.75 g EDTA (disodium salt)Adjust pH to 7.6 with acetic acid. (Approx. 20 mL)20X SSCto make one liter:175.3 g NaCl88.2 g NaCitrateadd water to bring volume to one liter.adjust to pH 7.0 with HCl.1% SDS, 0.2 M NaOHto make 100 mL:93 mL ddH205 mL 20% SDS2 mL 10 M NaOH14% PEG (8000), 2M NaCl, 10 mM MgSO4 to make one liter:140 g PEG117 g NaCl2.46 g MgSO4For use in phage DNA preparation.20% SDSto male 250 mL:50 g of SDS in a beakerAdd stir bar and H20 last.This solution will have to be heated for the SDS to dissolve.1.0 M Tris, pH 8.0, 1.5 M NaClto make one liter:121.1 g Trizma87.6 g NaClin a volume of water less than 1L. Adjust pH with HCl, then bring to 1L with H2010 mM Tris-HCl, pH 7.5, 10 mM MgSO4to make one liter:10 mL 1 M Tris-HCl2.46 g MgSO4for use in phage DNA preparation10 mM Tris, 50 mM EDTA, pH 7.5to make 200 mL:2 mL 1 M Tris20 mL 0.5 M EDTA (tetrasodium salt)178 mL ddH20adjust pH with HCl.10 mM Tris-HCl, 1 mM EDTA, pH 7.5to make 200 mL:2.0 mL 1 M Tris-HCl, pH 7.50.4 mL 0.5 M EDTA197.6 mL ddH203 M Sodium Acetate, pH 4.8to make one liter:408.1 g NaAce (trihydrate; gets cold in soln)about 700 mL H20adjust pH with glacial acetic acid (takes a lot)Measure tru pH by dilution with water; range will be between 4.8 and 5.5.Electrophoresis Dyeto make 4 mL:3 mL 50 mM EDTA, 10 mM Tris-HCl, pH 8.01 mL glycerol20 μL BPB10 μL Xylene cyanolStop dye for labelled probe1 mL 50 mM EDTA, 10 mM Tris, pH 7.5-8.5about 200 μl glyceroladd a few grains of blue dextran (8000)Sequencing gel dyefor approx 1 mL:1 mL formamide10 μL xylene cyanol10 μl BPB3 μL 10 M NaOH5% acrylamideto make 200 mL:20 mL 10X TBE25 mL 40% acrylamide155 mL H206% Acrylamide in TBE, 50% Ureato make 500 mL:50 mL 10X TBE75 mL 40% acrylamide250 g Ureabring to 500 mL with H2O40% Acrylamide (38:2 acrylamide:bis acrylamide) to make 200 mL:76 g acrylamide4 g bis acrylamidebring to 200 mL with H2O。

Product Data SheetPerkadox 16Di(4-tert-butylcyclohexyl) peroxydicarbonatePerkadox® 16 is applied as an initiator for the suspension and mass polymerization of vinyl chloride in the temperature range between 40°C and 65°C. Perkadox® 16 can be used alone or in combination with other peroxides, such as 1,1,3,3-Tetramethylbutyl peroxyneodecanoate (Trigonox 423), Cumyl peroxyneodecanoate (Trigonox 99) or Dilauroyl peroxide (Laurox), to increase reactor efficiency.CAS number15520-11-3EINECS/ELINCS No.239-557-1TSCA statuslisted on inventoryMolecular weight398.5SpecificationsAppearance White powderAssay94.0-97.0 %Inorganic + organic hydrolysable chloride≤ 4000 mg/kgCharacteristicsBulk density, 20 °C450-480 kg/m³Density, 20 °C 1.13 g/cm³ApplicationsPerkadox® 16 can be used for the market segments: polymer production, thermoset composites and acrylics with their different applications/functions. For more information please check our website and/or contact us.Half-life dataThe reactivity of an organic peroxide is usually given by its half-life (t½) at various temperatures. For Perkadox® 16 in chlorobenzene:0.1 hr82°C (180°F)1 hr64°C (147°F)10 hr48°C (118°F)Formula 1kd = A·e-Ea/RTFormula 2t½ = (ln2)/kdEa126.39 kJ/moleA7.44E+15 s-1R8.3142 J/mole·KT(273.15+°C) KThermal stabilityOrganic peroxides are thermally unstable substances, which may undergo self-accelerating decomposition. The lowest temperature at which self-accelerating decomposition of a substance in the original packaging may occur is the Self-Accelerating Decomposition Temperature (SADT). The SADT is determined on the basis of the Heat Accumulation Storage Test.SADT40°CEmergency temperature (Tₑ)35°CControl temperature (Tc)30°CMethod The Heat Accumulation Storage Test is a recognized test method for thedetermination of the SADT of organic peroxides (see Recommendations on theTransport of Dangerous Goods, Manual of Tests and Criteria – United Nations,New York and Geneva).StorageDue to the relatively unstable nature of organic peroxides a loss of quality can be detected over a period of time. To minimize the loss of quality, Nouryon recommends a maximum storage temperature (Ts max. ) for each organic peroxide product.Ts max.20°C (please see note below)Note When stored under the recommended storage conditions, Perkadox® 16 willremain within the Nouryon specifications for a period of at least 3 months afterdelivery. The Ts max of 20°C is not to be interpretated as ambient or roomtemperature as this differs per region and season. Perkadox® 16 has a high qualitycomposition and to hold that it should be stored at below 20°C. At temperaturesabove 20°C the decomposition of Perkadox® 16 progresses fast which leads tosignificant loss of quality. If you have questions about this, please contact yourlocal Nouryon account manager for advice.Packaging and transportIn North America Perkadox® 16 is packed in non-returnable cartons containing 25 polyethylene bags of 1 lb net weightor 5 polyethylene bags of 5 lb net weight. In other regions the standard packaging is a cardboard box for 20 kg peroxide. Both packaging and transport meet the international regulations. For the availability of other packed quantities contact your Nouryon representative. Perkadox® 16 is classified as Organic peroxide type C; solid, temperature controlled; Division 5. 2; UN 3114.Safety and handlingKeep containers tightly closed. Store and handle Perkadox® 16 in a dry well-ventilated place away from sources of heat or ignition and direct sunlight. Never weigh out in the storage room. Avoid contact with reducing agents (e. g. amines), acids, alkalis and heavy metal compounds (e. g. accelerators, driers and metal soaps). Please refer to the Safety Data Sheet (SDS) for further information on the safe storage, use and handling of Perkadox® 16. This information should be thoroughly reviewed prior to acceptance of this product. The SDS is available at /sds-search.Major decomposition products Carbon dioxide, 4-tert-Butyl-cyclohexanolAll information concerning this product and/or suggestions for handling and use contained herein are offered in good faith and are believed to be reliable.Nouryon, however, makes no warranty as to accuracy and/or sufficiency of such information and/or suggestions, as to the product's merchantability or fitness for any particular purpose, or that any suggested use will not infringe any patent. Nouryon does not accept any liability whatsoever arising out of the use of or reliance on this information, or out of the use or the performance of the product. Nothing contained herein shall be construed as granting or extending any license under any patent. Customer must determine for himself, by preliminary tests or otherwise, the suitability of this product for his purposes.The information contained herein supersedes all previously issued information on the subject matter covered. The customer may forward, distribute, and/or photocopy this document only if unaltered and complete, including all of its headers and footers, and should refrain from any unauthorized use. Don’t copythis document to a website.Perkadox® and Trigonox are registered trademarks of Nouryon Functional Chemicals B.V. or affiliates in one or more territories.Contact UsPolymer Specialties Americas************************Polymer Specialties Europe, Middle East, India and Africa*************************Polymer Specialties Asia Pacific************************2023-1-10© 2023Polymer production Perkadox 16。

H&DEzetimibeTetrahydropyran Impurity (SCH59566)H&DEzetimibe Impurity((2R,3S)-2-[(4-Benzyloxyphenyl)-4-(4-Fluorophenylamino)m ethyl]pentanedioicacid)H&DEzetimibe(3R,4R,3'R)-isomerH&DEzetimibeAzetidinone Ring-opened Impurity深圳市恒丰万达医药科技有限公司CAS：3222-33-1中文名称：伊泽替米贝杂质英文名称：Ezetimibe分子式：C24H21F2NO3规格：10-100mg纯度：>95%用途：供实验室用H&DEzetimibe(3S,4S,3'S)-isomerH&D Ezetimibe FluoroIsomerH&DEzetimibe Impurity((2R,3S)-2-[(4-Benzyloxyphenyl)-4-(4-Fluorophenylamino)m ethyl]pentanedioicacid)H&D Ezetimibe Triol ImpurityH&DEzetimibeDeprotected Impurity ((S)-3-{(2R, 5S)-5-(4-Fluorophenyl)-2-[(S)-(4-Fluorophenylamino)-(4-OH-phenyl)methyl]-5-OH&D(R)-Ezetimibe Desfluoro ChloroImpurityH&D Ezetimibe Desfluoro Dehydoxy ImpurityH&D Ezetimibe Impurity9H&D Ezetimibe AnilineImpurityH&D Benzyl Ezetimibe Diol (Mixture of Diastereomers)依泽替米贝副作用;别名:依泽替米倍、依折麦布、Ezetrol、Zetia;依泽替米贝适应症:1.单独用于原发性高胆固醇血症：作为饮食控制的辅助治疗用于家族性高胆固醇血症的初期治疗，降低总胆固醇、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白B浓度。

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:Y–27632 is an ATP–competitive inhibitor of ROCK–I and ROCK–II , with K i of 220 nM and 300 nM for ROCK–I and ROCK–II , respectively.IC50 & Target: Ki: 220/300 nM (ROCK–I/II)[1]In Vitro: Y–27632 inhibits the ROCK family of kinases 100 times more potently than other kinases including protein kinase C,cAMP–dependent kinase and myosin light chain kinase. Y–27632 prolongs the lag time and delays the appearance of BrdU–labeled cells in a concentration–dependent manner, delays of about 1 and 4 h are noticed in the Swiss 3T3 cells treated with 10 and 100 μM Y–27632, respectively [1]. Y–27632 promotes neuronal differentiation of adipose tissue–derived stem cells (ADSCs). Compared to 1.0and 2.5 μM Y–27632 induced groups, percentages of neuroal–like cells achieved a peak in the 5.0 μM Y–27632 induced group [2].In Vivo: Y–27632 (5 and 10 mg/kg) significantly prolongs the onset time of myoclonic jerks when compare with saline group.Y–27632 (5 and 10 mg/kg) significantly prolongs the onset time of clonic convulsions when compare with saline group [3].Treatment with Dimethylnitrosamine (DMN) causes a significant decrease in rat body and liver weight (DMN–S group) compared with control animals (S–S group). Oral Y27632 (30 mg/kg) essentially prevents this DMN–induced rat body and liver weight loss (DMN–Y group)[4].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]Recombinant ROCK–I, ROCK–II, PKN, or citron kinase is expressed in HeLa cells as Myc–tagged proteins by transfection using Lipofectamine, and is precipitated from the cell lysates by the use of 9E10 monoclonal anti–Myc antibodycoupled to G protein–Sepharose. Recovered immunocomplexes are incubated with various concentrations of [32P]ATP and 10 mg of histone type 2 as substrates in the absence or presence of various concentrations of either Y–27632 or Y–30141 at 30°C for 30 min in a total volume of 30 μL of the kinase buffer containing 50 mM HEPES–NaOH, pH 7.4, 10 mM MgCl 2, 5 mM MnCl 2, 0.02% Briji 35, and 2 mM dithiothreitol. PKCa is incubated with 5 μM [32P]ATP and 200 μg/mL histone type 2 as substrates in the absence or presence of various concentrations of either Y–27632 or Y–30141 at 30°C for 10 min in a kinase buffer containing 50 mM Tris–HCl,pH 7.5, 0.5 mM CaCl 2, 5 mM magnesium acetate, 25 μg/mL phosphatidyl serine, 50 ng/mL 12–O–tetradecanoylphorbol–13–acetate and 0.001% leupeptin in a total volume of 30 μL. Incubation is terminated by the addition of 10 μL of 43 Laemmli sample buffer.After boiling for 5 min, the mixture is subjected to SDS–polyacrylamide gel electrophoresis on a 16% gel. The gel is stained withCoomassie Brilliant Blue, and then dried. The bands corresponding to histone type 2 are excised, and the radioactivity is measured [1]. Cell Assay: Y–27632 is dissolved in water and stored [1].[1]HeLa cells are plated at a density of 3×104 cells per 3.5–cm dish. The cells are cultured in DMEM containing 10% FBS in the presence of 10 mM Thymidine for 16 h. After the cells are washed with DMEM containing 10% FBS, they are cultured for an additional 8 h, and then 40 ng/mL of Nocodazole is added. After 11.5 h of theNocodazole treatment, various concentrations of Y–27632 (0–300 μM), Y–30141, or vehicle is added and the cells are incubated for another 30 min [1].Animal Administration: Y–27632 is dissolved in 0.9% NaCl (saline) (Mice)[3].Product Name:Y–27632Cat. No.:HY-10071CAS No.:146986-50-7Molecular Formula:C 14H 21N 3O Molecular Weight:247.34Target:ROCK; ROCK; ROCK Pathway:TGF–beta/Smad; Stem Cell/Wnt; Cell Cycle/DNA Damage Solubility:DMSO: ≥ 32 mg/mLY–27632 is dissolved in saline (final concentration 2%) (Rat)[4].[3][4]Mice[3]Male, inbred Swiss albino mice (2–3 months old) weighing 25–30 g are used. Mice are injected with a sub–convulsive dose of PTZ (35 mg/kg, i.p.) (on Mondays, Wednesdays and Fridays) of each week for a total of 11 injections. After each PTZ injection, mice are observed for 30 min and the occurrence of convulsive activity is recorded. After 30 min, the mice are then injected with either Fasudil (25 mg/kg, i.p.) or Y–27632 (5 mg/kg, i.p.) and returned to their home cages until the next injection. Control mice for Fasudil andY–27632 receives saline.Rat[4]Male Wistar Kind A rats (200–250 g) are used. DMN (1 g/mL) is diluted ten times with saline (final concentration 1%) and 10 mg/kg per day of DMN is injected intraperitoneally (i.p.) on the first 3 days of each week for 4 weeks. Y27632 is given orally once per day at a dose of 30 mg/kg for 4 weeks starting on the day of the first injection of DMN. The dose of 30 mg/kg corrects hypertension in several rat models without toxicity. Twenty rats are randomized into four experimental groups (n=5 in each group) as follows: (1) S–S (injection of saline i.p. and oral administration of saline); (2) S–Y (injection of saline i.p. and oral administration of Y27632); (3) DMN–S (DMN i.p. and oral administration of saline); (4) DMN–Y (DMN i.p. and oral administration of Y27632). The rats are weighed every week. They are sacrificed at the end of the fourth week and the liver is excised. In addition, a blood sample is taken immediately before the rats are sacrificed.References:[1]. Ishizaki T, et al. Pharmacological properties of Y–27632, a specific inhibitor of rho–associated kinases. Mol Pharmacol. 2000 May;57(5):976–83.[2]. Xue ZW, et al. Rho–associated coiled kinase inhibitor Y–27632 promotes neuronal–like differentiation of adult human adipose tissue–derived stem cells.Chin Med J (Engl). 2012 Sep;125(18):3332–5.[3]. Inan S, et al. Antiepileptic effects of two Rho–kinase inhibitors, Y–27632 and fasudil, in mice. Br J Pharmacol. 2008 Sep;155(1):44–51.[4]. Tada S, et al. A selective ROCK inhibitor, Y27632, prevents dimethylnitrosamine–induced hepatic fibrosis in rats. J Hepatol. 2001 Apr;34(4):529–36.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

分子量409.4溶解性（25°C ）DMSO 82 mg/mL 分子式C H F NO Water <1 mg/mL CAS 号163222-33-1Ethanol 82 mg/mL储存条件3年 -20°C 粉末状生物活性Ezetimibe (Zetia) is a compound that lowers cholesterol. Ezetimibe (Zetia) acts by decreasing cholesterol absorption in the intestine. Ezetimibe (Zetia) may be used alone, when other cholesterol-lowering medications are not tolerated, or together with statins when statins alone do not control cholesterol. Ezetimibe localises at the brush border of the small intestine, where ezetimibe (Zetia) inhibits the absorption of cholesterol from the intestine. Specifically, ezetimibe (Zetia)appears to bind to a critical mediator of cholesterol absorption, the Niemann-Pick C1-Like 1 (NPC1L1) protein on the gastrointestinal tract epithelial cells as well as in hepatocytes.不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA 指南）小鼠大鼠兔豚鼠仓鼠狗重量 (kg)0.020.15 1.80.40.0810体表面积 (m )0.0070.0250.150.050.020.5K 系数36128520动物 A (mg/kg) = 动物 B (mg/kg) ×动物 B 的K 系数动物 A 的K 系数例如，依据体表面积折算法，将白藜芦醇用于小鼠的剂量22.4 mg/kg 换算成大鼠的剂量，需要将22.4 mg/kg 乘以小鼠的K 系数（3），再除以大鼠的K 系数（6），得到白藜芦醇用于大鼠的等效剂量为11.2 mg/kg 。