ADTT1-1

胸苷激酶1的研究进展邢晓光【摘要】恶性肿瘤是威胁人类健康最主要的疾病之一,目前主要靠病理学及影像学来诊断,只有患者具有明显占位性病变和临床症状后才能确诊,而此时已非癌变最早期,且已过最佳治疗时期.因此,对恶性肿瘤具有早期诊断作用的血清肿瘤标志物是目前研究的重点.随着现代血清学肿瘤标志物相关研究的迅速发展,一种新的细胞增殖标志物,即胸苷激酶1(TK1)被证实在肿瘤早期发现、疗效评估、预后判断等方面具有重要意义.现从TK1的结构、功能、与DNA合成及肿瘤的关系等方面予以综述.%Malignant tumor is the most important disease threatening human health, current major di-agonsis of which is by pathology and imaging; affirmative diagnosis can only be achieved when patients have obvious space-occupying lesions and clinical symptoms, missing the early stage of canceration, which is the optimal treatment time. Therefore it is the current research focus to find serum tumor marker for early diagnosis of malignant tumor. With the rapid development of the serum tumor marker,thymidine kinase-l( TK1 ),a new cell proliferation marker, has been confirmed to be of great significance in early detection, therapeutic effect evaluation and prognosis of tumors. Here is to make a review on TK1 structure, function and its correlation with DNA synthesis and tumors.【期刊名称】《医学综述》【年(卷),期】2012(018)001【总页数】4页(P66-69)【关键词】胸苷激酶1;恶性肿瘤;细胞增殖标志物【作者】邢晓光【作者单位】天津港口医院检验科,天津,300456【正文语种】中文【中图分类】R730恶性肿瘤是威胁人类健康最主要的疾病之一，对其早期诊断更具临床意义。

Page 1 of 2APPLICATIONThe ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack supports 10GBASE-T networks. The jack is part of a complete ATLAS-X1 Cat 6A UTP system, ideal for the most demanding mission-critical network applications. The connector supports emerging technologies and will easily adapt to network trends.SPECIFICATIONThe jack shall meet or exceed the requirements for channel and component-level electrical transmission performance as described in ANSI/TIA-568.2-D (Cat 6A), ISO/IEC11801-1 (Class E A ), and EN 50173-1 (Class E A ). The jack shall be compliant with ANSI/TIA-1096-A, c(UL)us Listed, and be independently verified for electrical transmission performance and power delivery. The jack body shall be made of die-cast zinc and all plastic components shall be made of high-impact, fire-retardant plastic rated UL 94V-0. The jack shall support tool-free termination and re-termination and shall not require a specialized termination tool. The jack wiring shall be universal to accommodate T568A and T568B wiring schemes. The jack shall be available in 13 colors; more than established by the ANSI/TIA-606-C standard. The jack shall be offered in standard and shuttered styles and select jacks shall be supplied with interchangeable icons. The jack shall be compliant with IEEE 802.3 PoE Type 1, 2, 3, 4 (100 watts max).DESIGN CONSIDERATIONS• Use in any QUICKPORT™ housing to support Cat 6A UTP connectivity in surface-mount, flush-mount, or modular furniture outlets and field-configurable panels• Can be used in conjunction with other QUICKPORT snap-in modules for voice/data/video applications over UTP , coax, and fiber• To identify ports, use different colored modules andicons for each application (full selection of ANSI/TIA-606 compatible colors, 13 available)• Robust housing and shutter protects the jack in harsh environmentsATLAS-X1™ Cat 6A Component-Rated UTP QUICKPORT™ Jack6AUJK-xx6, ICONS-ICxFEATURES• Independently tested and guaranteed to exceed all component, permanent link, and channel margins• Patented Retention Force Technology™ (RFT) protects against tine damage and increases system longevity • For Power over Ethernet, RFT maintains contact force between plug and jack, preventing arcing from intermittent disconnects• Unique design supports tool-free termination andre-termination and requires no specialized termination tool • Short jack design supports a wider range of applications (e.g. shallow boxes, enclosures, bend radius, etc.)• Terminates from 26 to 22 AWG solid or stranded conductors for use on various cable types• Robust IDCs can withstand 20 re-termination cycles and jack contacts are tested for 750 plug-mating cycles to ensure system longevity• Available in 13 ANSI/TIA-606-C compatible colors • Tested and approved for use in air-handling spaces (plenum rating) in accordance with UL Standard 2043• Select jacks available with interchangeable icons (voice, data, A/V, blank) for easy ID• Jack with internal shutter protects against dust and debris • Solid metal body dissipates 53% more heat than plastic, minimizing damage from excess heat in PoE applications • Tine geometry prevents arcing damage where plug and jack make contactSTANDARDS & REGULATIONS• ANSI/TIA-568.2-D (Cat 6A)• ISO/IEC 11801-1 (Cat 6A)• EN 50173-1 (Cat 6A)• ANSI/TIA-1096-A (formerly FCC Part 68)• IEC 60603-7 (includes IEC 60512-5-2)• IEC 60512-99-002• IEEE 802.3 PoE Type 1, 2, 3, 4 (100 watts max)• Cisco UPOE, UPOE+ (90 watts max)• Power over HDBaseT™ PoH (95 watts max)• c(UL)us Listed (UL 1863)• UL 2043 Plenum Certified • RoHS 3• ETL verified to meet the IEC 60512-99-002 standard for support of IEEE 802.3 Type 4 PoE (100 watt) applicationsCOUNTRY OF ORIGINUSA and Mexico (Contact Customer Service for details)6AUJK-xx6, ICONS-ICxUSANetwork Solutions Headquarters +1 (800) 722 2082 *******************Leviton Berk-Tek Cable : +1 (800) 237 5835 ************************Asia Pacific+852 3620 2602********************Canada+1 (800) 461 2002**********************Europe+44 (0) 1592 772124 **********************Latin AmericaMX: +52 (55) 2128 6286 LATAM: +52 (55) 2333 5963 *********************Middle East & Africa +971 (4) 247 9800 *******************NETWORK SOLUTIONS PRODUCTS ARE AVAILABLE WORLDWIDE IN OVER 100 COUNTRIES. VISIT US ONLINE AT /NS TO LEARN MORE.Page 2 of 2For further support information, visit /ns/support6AUJK-xx6, ICONS-ICx6AUJK-xx6, ICONS-ICxMECHANICAL SPECIFICATIONSDimensions:See belowMaterials: Jack Body: Die-cast zincSpring-Wire Contacts: High quality, copper-based alloy, plated with 50 microinches of gold for lowest contact resistance andmaximum life Temp. (Storage):Temp. (Installation): Temp. (Operating):Humidity (Max.):WARRANTY INFORMATIONFor Leviton product warranties, go to /ns/warrantyPART NUMBERDescriptionStandard Jack Jack with Shutter GREENPACK™12-Pack Standard Jack ATLAS-X1™ Cat 6A Component-Rated UTP QUICKPORT™ Jack, white 6AUJK-RW66AUJK-SW66AUJK-CW6ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, light almond 6AUJK-RT66AUJK-ST6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, ivory 6AUJK-RI66AUJK-SI6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, yellow 6AUJK-RY66AUJK-SY6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, orange 6AUJK-RO66AUJK-SO6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, crimson 6AUJK-RC66AUJK-SC6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, dark red 6AUJK-RR66AUJK-SR6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, purple 6AUJK-RP66AUJK-SP6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, blue 6AUJK-RL66AUJK-SL66AUJK-CL6ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, green 6AUJK-RV66AUJK-SV6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, gray 6AUJK-RG66AUJK-SG6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, black6AUJK-RE66AUJK-SE66AUJK-CE6ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, brown6AUJK-RB66AUJK-SB6—Green (V)Blue (L)Purple (P)Crimson (C)Dark Red (R)Orange (O)Yellow (Y)Black (E)Gray (G)Ivory (I)Light Almond (T)Brown (B)Color-matched icons (ICONS-ICx) can be ordered separately in 72-quantity packs.x = icon color。

ｅｘｐｒｅｓｓｉｏｎｓｏｆＺＮＲＦ２ｍＲＮＡａｎｄｐｒｏｔｅｉｎｗｅｒｅｄｏｗｎ ｒｅｇｕｌａｔｅｄｓｉｇｎｉｆｉｃａｎｔｌｙ．Ｓｉｍｕｌｔａｎｅｏｕｓｌｙ，ｔｈｅｐｒｏｔｅｉｎｓｅｘ ｐｒｅｓｓｉｏｎｓｏｆＬＣ３ＩＩａｎｄＢｅｃｌｉｎ １ｉｎｃｒｅａｓｅｄ，ａｎｄｔｈｅｅｘ ｐｒｅｓｓｉｏｎｏｆｐ６２ｐｒｏｔｅｉｎｄｅｃｒｅａｓｅｄｉｎＯＧＤ／Ｒｇｒｏｕｐ．ＣｏｍｐａｒｅｄｗｉｔｈＯＧＤ／Ｒｇｒｏｕｐ，ｔｈｅｃｅｌｌｖｉａｂｉｌｉｔｙｗａｓｅｎ ｈａｎｃｅｄ，ｔｈｅｃｅｌｌａｐｏｐｔｏｓｉｓａｎｄａｕｔｏｐｈａｇｙｗｅｒｅｄｅ ｃｒｅａｓｅｄｉｎＬＶ ＺＮＲＦ２ｇｒｏｕｐ．Ｉｎｃｏｎｔｒａｓｔ，ｔｈｅｃｅｌｌｖｉａ ｂｉｌｉｔｙｄｅｃｒｅａｓｅｄａｎｄｔｈｅｃｅｌｌａｐｏｐｔｏｓｉｓａｎｄａｕｔｏｐｈａｇｙｗｅｒｅａｇｇｒａｖａｔｅｄａｆｔｅｒｔｒａｎｓｆｅｃｔｉｎｇｓｉＲ ＺＮＲＦ２．Ｃｏｎ ｃｌｕｓｉｏｎｓ　ＺＮＲＦ２ｐｒｏｔｅｃｔｓＰＣ１２ｃｅｌｌｓｆｒｏｍｔｈｅｉｎｊｕｒｙｃａｕｓｅｄｂｙＯＧＤ／Ｒａｎｄｉｔｓｍｅｃｈａｎｉｓｍｍａｙｂｅｒｅｌａｔｅｄｔｏｔｈｅｉｎｈｉｂｉｔｉｏｎｏｆａｕｔｏｐｈａｇｙ．Ｋｅｙｗｏｒｄｓ：ｏｘｙｇｅｎ ｇｌｕｃｏｓｅｄｅｐｒｉｖａｔｉｏｎ／ｒｅｏｘｙｇｅｎａｔｉｏｎ；ＰＣ１２ｃｅｌｌｓ；ＺＮＲＦ２；ａｕｔｏｐｈａｇｙ；ｍＴＯＲ；ａｐｏｐｔｏｓｉｓ网络出版时间：２０２１－５－２８１０：１２　网络出版地址：ｈｔｔｐｓ：／／ｋｎｓ．ｃｎｋｉ．ｎｅｔ／ｋｃｍｓ／ｄｅｔａｉｌ／３４．１０８６．Ｒ．２０２１０５２７．１４５８．０１６．ｈｔｍｌ长链非编码ＲＮＡＫｃｎｑ１ｏｔ１对小鼠糖尿病心肌病的作用杨　帆１，王丽宏２（１．南京大学医学院附属鼓楼医院内分泌科，江苏南京　２１０００８；２．哈尔滨医科大学附属第二医院内分泌科，黑龙江哈尔滨　１５０００１）ｄｏｉ：１０．３９６９／ｊ．ｉｓｓｎ．１００１－１９７８．２０２１．０６．００８文献标志码：Ａ文章编号：１００１－１９７８（２０２１）０６－０７７４－０７中国图书分类号：Ｒ ３３２；Ｒ３２２ １１；Ｒ３４２ ２；Ｒ５４２ ２；Ｒ５４２ ２０２ ５；Ｒ５８７ ２摘要：目的　研究长链非编码ＲＮＡＫｃｎｑ１ｏｔ１在糖尿病心肌病中的作用及机制。

探讨ET-1能否引起大鼠心肌自由基含量的增高-病理学论文-基础医学论文-医学论文——文章均为WORD文档，下载后可直接编辑使用亦可打印——内皮素－1(endothelin－1，ET－1)是心血管系统中ET的主要形式，可由内皮细胞、血管平滑肌细胞、心肌细胞、肝细胞和星形胶质细胞等合成。

ET－1在心律失常、慢性心力衰竭、动脉粥样硬化等多种心血管疾病发病中都起着重要作用。

氧自由基是机体内氧分子的不完全代谢产物，在缺血、缺氧等应激刺激下，可导致氧自由基生成过多或清除能力减弱，体内积累过多的氧自由基可对体内大分子物质产生毒害，直接或间接导致心血管疾病的发生。

此前我们已报道超氧化物歧化酶(superoxidedismutase－1，SOD－1)能明显减轻ET－1引起大鼠心律失常，本实验应用电子自旋共振(ESR)方法经大鼠检测冠状动脉口注射外源性ET－1后对大鼠心肌氧自由基含量的影响，以明确ET－1能否引起大鼠心肌自由基含量的增高。

1、材料与方法1．1实验材料1．1．1动物Sprague－Dawley大鼠30只，雄性，体重(20020)g，由大学医学部实验动物中心提供。

1．1．2仪器E－200电子自旋共振仪(X波段，德国Brucker 公司)。

1．1．3试剂ET－1(日本大阪肽研究所公司);超氧化物歧化酶(SOD－1，南京建成生物公司)。

1．2实验方法1．2．1分组30只雄性SD大鼠，随机分为3组，每组10只。

ET－1组:冠状动脉口注射1．5molL－1的ET－1900pmolkg－1;SOD－1+ET－1组:冠状动脉口注射SOD－10．6mgkg－14min后，再注射1．5molL－1的ET－1900pmolkg－1(各组大鼠ET－1的注射剂量均为0．6mLkg－1);对照组:冠状动脉口注射生理盐水0．6mLkg－1。

1．2．2冠状动脉口注射［6］大鼠用20%尿酯6mLkg－1腹腔注射麻醉，仰卧位固定，气管插管，分离右侧颈总动脉。

二硫代氨基甲酸盐缓蚀剂DTC-1的合成及性能研究I. 引言- 研究目的和意义- 国内外研究现状II. 实验部分- 合成技术路线- 合成方法及反应机理- 产物的表征和纯化III. 性能测试- 腐蚀性能测试方法- 缓蚀性能测试方法- 结果分析和比较IV. 机理研究- 缓蚀机理的探究- 确定缓蚀剂的作用机制V. 结论和展望- 综合分析实验结果和机理研究结果- DTC-1的应用前景和未来研究方向参考文献I. 引言缓蚀剂是一种常用的材料，在金属材料的防腐蚀方面具有广泛的应用。

近年来，随着腐蚀问题愈发突出，人们对缓蚀剂的研究和应用也越来越重视。

其中，二硫代氨基甲酸盐是目前应用较为广泛的缓蚀剂之一。

DTC-1即二甲基二硫代氨基甲酸二乙酯，是一种新型的二硫代氨基甲酸盐类缓蚀剂，其具有良好的缓蚀性能和水热稳定性，广泛应用于腐蚀保护、钢材的溶液防锈、防锈涂料等方面。

本文旨在研究DTC-1的合成及其缓蚀性能，探究其物化性质和缓蚀机理，为该类缓蚀剂的应用提供新的思路和方法。

目前，国内外对二硫代氨基甲酸盐缓蚀剂的研究较为活跃。

国内外许多学者从不同的角度和方向研究了这种化合物的合成、性能、机理等方面。

但是，目前研究大多数集中在缓蚀效果评价以及相关化合物的合成上，对于机理探究和优化改进研究不足，因此本文对DTC-1缓蚀剂进行了合成和性能研究，并对其缓蚀机理进行了初步探究，为相关领域研究提供一定的借鉴和参考。

本文的内容主要包括三个部分：实验部分、性能测试和机理研究部分。

在实验部分，我们将介绍DTC-1的合成方法和反应机理；在性能测试部分，我们将通过不同的腐蚀性能测试和缓蚀性能测试来评价DTC-1的表现；在机理研究部分，我们将探究DTC-1缓蚀的机理，并寻找适合其应用的改进策略。

最后，我们将综合分析实验结果和机理研究结果，对DTC-1的应用前景和未来研究方向进行展望。

II. 实验部分A. 合成技术路线DTC-1的合成主要是通过二乙酸二甲酯与硫代氨基甲酸双分子进行反应得到。

针对小于或等于150磅的包装产品ISTA（国际运输安全协会）1系列非模拟整体测试程序ISTA（国际运输安全协会），您运输包装的合作伙伴，是全世界包装成品性能测试中的引领者ISTA（国际运输安全协会）测试系列是产品性能测试最基本的一类测试，主要针对包装及产品对运输损坏抵抗力测试，但不是指对产品的实际运输损坏的模拟，且并不一定严格符合承运者实际包装规则如果操作恰当，ISTA（国际运输安全协会）能够提供很多实际性的利益，如缩短包装时间，增加产品可信度产品保护，减少产品损伤，带来利润经济实惠的分销成本令客户满意，并维持长期交易共分为三个部分：概述，测试和报告概述部分讲了实验室测试前的基本知识测试部分讲了实验测试时的具体操作行动指令报告部分讲了记录的数据资料报告应递交到ISTAISTA（国际运输安全协会）测试程序中提供了两个重量和尺寸大小版本系统。

即英制版本（英寸－磅）及国际单位版本（米制）。

括号内提供了英寸－磅单位与米制单位，其他一些表格中则分开显示每一个单位系统版本均可选择，但选择其中一种单位系统后，整个测试程序中应保持一致，不能再选择另外一个单位系统。

两个单位均能够转换成实际意义的数据，但不是绝对相等非常重要：测试前须阅读并理解整个文件序言测试程序1A概述测试程序1A是针对单个包装产品的整体测试能够用来评估产品性能能够用于比较包装及产品设计更改后的相关性能包装与产品视为一个整体，不分开考虑未包括诸如潮湿，受压及异常搬运等运输条件状况其他一些ISTA（国际运输安全协会）程序适合于不同的条件或满足其他目的具体建议：实施随机振动而非固定位移，使用ISTA（国际运输安全协会）测试程序1G而非1A使用ISTA（国际运输安全协会）测试程序1C来测量包装产品的最小抗压值使用ISTA（国际运输安全协会）部分即时性能测试程序2A来测量国际运输分销产品使用ISTA（国际运输安全协会）全面即时性能测试程序3A来测量小包袋运输包装产品更多信息，请参考选用ISTA（国际运输安全协会）测试程序项目指导测试程序1A概述测试范围测试程序1A涵盖了重量小于或等于150磅（68千克）单个包装运输产品测试例外：对于置于卡板上其重量大于100磅(45千克)的单个包装产品，应按照测试程序1E进行产品损伤的容许及降级接受测试前承运人应确定什么构成对产品的损伤什么程度范围内损伤可容许测试结论中决定产品条件的正确方法测试结论中可接受的包装条件更多信息，请参考选用ISTA（国际运输安全协会）测试程序项目指导―――――――――――――――――――――――――――――――――――――――测试样品测试样品应是未经测试的实际包装和产品。

DNA甲基化羟化酶TET1的研究进展于建才;王伟;赵娜【摘要】TET1作为TET蛋白家族的一员,是一种α-酮戊二酸和Fe2+依赖的双加氧酶,能转化5-甲基胞嘧啶(5mC)为5-羟甲基胞嘧啶(5hmC),从而启动DNA去甲基化程序.TET1既能对胚胎干细胞分化基因保持沉默,又能使其多能性基因保持激活,这种双管齐下的调控方式保持了胚胎干细胞自我更新能力和多能性潜力.TET1与肿瘤、精神疾病及白血病等疾病密切相关,因此TET1的研究对于拓展DNA去甲基化、胚胎干细胞基因调控及探求临床疾病治疗新靶向具有潜在价值.【期刊名称】《医学综述》【年(卷),期】2014(020)022【总页数】3页(P4051-4053)【关键词】TET1;DNA甲基化羟化酶;胚胎干细胞调控;肿瘤【作者】于建才;王伟;赵娜【作者单位】哈尔病医科大学附属第一医院心内八科,哈尔滨150001;承德医学院研究生院,河北承德067000;哈尔病医科大学附属第一医院心内八科,哈尔滨150001【正文语种】中文【中图分类】Q756DNA甲基化是一种重要的表观遗传修饰，在胚胎发育、造血系统及肿瘤等相关疾病发生过程中起重要作用[1]。

高等动物体内DNA甲基化主要是指DNA甲基转移酶介导的在CpG二核苷酸胞嘧啶第5位碳原子上加入甲基基团转变为5-甲基胞嘧啶(5-methylcytosine，5mC)的修饰过程[2]，并且这个动态过程可以逆转[3]。

TET(ten eleven translocation)1是一种5mC羟基化酶，能启动DNA去甲基化程序，调控胚胎干细胞的发育，并与肿瘤、精神、血液系统等疾病发病密切相关。

1 TET1的基本特征TET家族是重新调控已经分化细胞的一种重要功能蛋白，在DNA去甲基过程和干细胞编程、抑制肿瘤细胞增殖等方面起关键作用[4]。

人类TET蛋白家族共有3名成员：TET1，TET2和TET3，其中TET1发现最早，由Tahiliani等[5]在研究1例存在t(10,11)(q22，q23)异位的白血病患者中作为融合蛋白发现的。

一种基于AD9959的频率合成器的设计闫朝政;田克纯;吴子勇;郭智威【摘要】直接数字频率合成(Direct Digital Synthesizer,DDS)利用了全数字的结构,通过DAC把数字量的信号转换为模拟量的信号,从而合成所需的模拟频率.DDS 拥有直接频率合成技术与间接式频率合成技术的优势.AD9959是一个内置了4个10 bit速度最高可达500 MS/s采样率的DAC的DDS芯片.以DDS原理为依据,采用AD9959芯片,以STC10L08XE单片机为MCU,设计了一种产生高频本振信号的频率合成器,有效应用在音频信号接收机中.【期刊名称】《电声技术》【年(卷),期】2013(037)002【总页数】6页(P25-29,32)【关键词】直接数字频率合成技术;AD9959;频率【作者】闫朝政;田克纯;吴子勇;郭智威【作者单位】桂林电子科技大学,广西桂林541004;桂林电子科技大学,广西桂林541004;桂林电子科技大学,广西桂林541004;桂林电子科技大学,广西桂林541004【正文语种】中文【中图分类】TN9121 引言现在音频信号是数字格式进行存储，而采集到的音频样本是模拟格式，在将模拟格式的音频信号进行数字化A/D之前，需要混频器对模拟音频信号进行下变频，以降低A/D采样率，减少A/D的复杂度，节约成本。

而频率合成器则为混频器提供本振信号源。

直接数字频率合成技术(DDS)是根据相位概念直接合成所需的频率，DDS具有频率转换快的特点，而且频率在一定的范围内可以适当地扩展，能够输出宽带本振信号，同时在一定程度上抑制杂散。

该设计是音频处理接收机模拟前端模块的本振源。

2 直接数字频率合成(DDS)的工作原理和结构直接数字频率合成技术可提供快速的信号建立时间，纯净的信号频谱［1］。

DDS 技术是通过DAC把数字量的信号转换为模拟量的信号，从而合成所需的频率。

DDS的结构如图1所示。

图1 DDS的框图结构在DDS工作时，在参考时钟的触发之下，相位累加器与频率控制字(Frequency Control Words，FCW)K在每一个时钟周期内相加一次，相加之后的相位值在每个周期内以二进制码的形式去寻址正弦查询表ROM，将相应的相位信息转化到相应的数字幅度值，ROM输出的数字量经过DAC之后转化为模拟量，如图2后半部分所示的阶梯波，再经过一个低通滤波器滤掉其他分量得到一个平滑的正弦波本振信号［2］。

DTS1242 Deadbreak Bolted T ee Connector: Interface C for Single Core Cable with Copper Wire Screen and Outer Sheath Installation InstructionsDISCLAIMER OF WARRANTIES AND LIMITATION OF LIABILITYThe information, recommendations, descriptions and safety notations in this document are based on Eaton Corporation’s (“Eaton”) experience and judgment and may not cover all contingencies. If further information is required, an Eaton sales office should be consulted. Sale of the product shown in this literature is subject to the terms and conditions outlined in appropriate Eaton selling policies or other contractual agreement between Eaton and the purchaser.THERE ARE NO UNDERSTANDINGS, AGREEMENTS, WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE OR MERCHANTABILITY, OTHER THAN THOSE SPECIFICALL Y SET OUT IN ANY EXISTING CONTRACT BETWEEN THE PARTIES. ANY SUCH CONTRACT STATES THE ENTIRE OBLIGATION OF EATON. THE CONTENTS OF THIS DOCUMENT SHALL NOT BECOME PART OF OR MODIFY ANY CONTRACT BETWEEN THE PARTIES. In no event will Eaton be responsible to the purchaser or user in contract, in tort (including negligence), strict liability or other-wise for any special, indirect, incidental or consequential damage or loss whatsoever, including but not limited to damage or loss of use of equipment, plant or power system, cost of capital, loss of power, additional expenses in the use of existing power facilities, or claims against the purchaser or user by its customers resulting from the use of the information, recommendations and descriptions contained herein. The information contained in this manual is subject to change without notice.ii InstallatIon InstructIons MN650023EN October 2016ContentsDISCLAIMER OF WARRANTIES AND LIMITATION OF LIABILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ii SAFETY FOR LIFE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iv SAFETY INFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .iv Safety instructions (iv)PRODUCT INFORMATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 Introduction (1)Read this manual first (1)Additional information (2)Acceptance and initial inspection (2)Handling and storage (2)Standards (2)INSTALLATION PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 Kit contents (2)Tools required (2)Cable preparation (1) (3)Adapter (2) (3)Crimp lugs (3) (4)Mechanical lugs (3) (4)Tee body (4) (5)Stud (5) (5)Tee (6) (5)Nut (7) (5)Plug (8) (5)Cap and earth wire (9) (5)iiiInstallatIon InstructIons MN650023EN October 2016iv DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screen and Outer SheathInstallatIon InstructIons MN650023EN October 2016Safety for lifeEaton meets or exceeds all applicable industry standards relating to product safety in its Cooper Power™ series products. We actively promote safe practices in the use and maintenance of our products through our service literature, instructional training programs, and the continuous efforts of all Eaton employees involved in product design, manufacture, marketing, and service.We strongly urge that you always follow all locally approved safety procedures and safety instructions when working around high voltage lines and equipment, and support our “Safety For Life” mission.Safety informationThe instructions in this manual are not intended as asubstitute for proper training or adequate experience in the safe operation of the equipment described. Only competent technicians who are familiar with this equipment should install, operate, and service it.A competent technician has these qualifications:●●Is thoroughly familiar with these instructions.●●Is trained in industry-accepted high and low-voltage safe operating practices and procedures.●●Is trained and authorized to energize, de-energize, clear, and ground power distribution equipment.●●Is trained in the care and use of protective equipment such as arc flash clothing, safety glasses, face shield, hard hat, rubber gloves, clampstick, hotstick, etc.Following is important safety information. For safeinstallation and operation of this equipment, be sure to read and understand all cautions and warnings.This manual may contain four types of hazard statements:Indicates an imminently hazardous situation which, ifnot avoided, will result in death or serious injury .Indicates a potentially hazardous situation which, if notavoided, could result in death or serious injury .Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury .CAUTIONIndicates a potentially hazardous situation which, if not avoided, may result in equipment damage only .Safety instructionsFollowing are general caution and warning statements that apply to this equipment. Additional statements, related to specific tasks and procedures, are located throughout themanual.Hazardous voltage . Contact with hazardous voltage will cause death or severe personal injury . Follow all locally approved safety procedures when working around high- and low-voltage lines and equipment .G103 .3Before installing, operating, maintaining, or testing this equipment, carefully read and understand the contents of this manual . Improper operation, handling ormaintenance can result in death, severe personal injury, and equipment damage .G101 .0This equipment is not intended to protect human life . Follow all locally approved procedures and safety practices when installing or operating this equipment . Failure to comply can result in death, severe personal injury and equipment damage .G102 .1Power distribution and transmission equipment must be properly selected for the intended application . Itmust be installed and serviced by competent personnel who have been trained and understand proper safety procedures . These instructions are written for suchpersonnel and are not a substitute for adequate training and experience in safety procedures . Failure to properly select, install or maintain power distribution and transmission equipment can result in death, severe personal injury, and equipment damage . G122 .21DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screenand Outer SheathInstallatIon InstructIonsMN650023EN October 2016All associated apparatus must be de-energized during any hands-on installation or maintenance . Failure to comply will result in death, severe personal injury andequipment damage .Eaton’s Cooper Power series separable connector is designed to be operated in accordance with normal safe operating procedures . These instructions are not intended to supersede or replace existing safety and operating procedures . Separable connectors must be de-energized during operation or maintenance . Visible break and adequate grounding must be provided before cable work proceeds . Ensure that the connector is rated for the intended application before it is installed .Separable connectors should be installed and serviced only by personnel familiar with good safety practice and the handling of high-voltage electrical equipment . Failure to comply could result in death, severe personalinjury and equipment damage .Use only voltage indicating instruments specifically designed for test points . Use of conventional voltage sensing devices may provide false “No Voltage” indications .The test point must be dry and free of contaminant’s when checking for voltage . After indication is taken: clean, dry, and lubricate the test point cap with silicone grease and assemble to the test point .The capacitive test point is not sufficiently accurate, nor is it intended for, actual voltage measurements or phasing operations .A reading of no voltage from the test point should not be the only indication of a de-energized circuit obtained before touching the connector . Other procedures can include direct conductor voltage testing or grounding using a live-line tool .Failure to comply could result in death, severe personalinjury and equipment damage .Figure 1 . Line illustration of 42 kV bolted tee connectorProduct informationIntroductionEaton’s Cooper Power series 1250 A, 42 kV bolted teeseparable connectors are used to terminate polymeric cable to equipment, such as transformers, switchgear, motors etc. equipped with bushings meeting type C interface per CENELEC EN 50180 and 50181. They are fully screened and fully submersible when mated with the proper bushing or plug and they meet the requirements of CENELEC HD 629.1 S2. The bolted tee connectors can be mounted vertically, horizontally or any angle in between with proper cable support. Each bolted tee connector is tested for AC withstand and partial discharge prior to leaving the factory.Read this manual firstRead and understand the contents of this manual and follow all locally approved procedures and safety practices before installing or operating this equipment.2DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screen and Outer SheathInstallatIon InstructIons MN650023EN October 2016Additional informationThese instructions cannot cover all details or variations in the equipment, procedures, or process described nor provide directions for meeting every possible contingency during installation, operation, or maintenance. For additional information, contact your representative.Acceptance and initial inspectionEach bolted tee connector is completely inspected and tested at the factory. It is in good condition when accepted by the carrier for shipment. Upon receipt of a bolted tee connector, inspect it thoroughly for damage and loss of parts incurred during shipment. If damage or loss is discovered, file a claim with the carrier immediately.Handling and storageBe careful during handling and storage of the bolted tee connector to minimize the possibility of damage. If the bolted tee connector is to be stored for any length of time prior to installation, provide a clean, dry storage area.StandardsISO 9001 Certified Quality Management SystemInstallation procedureKit contents1 - Tee connector (test point optional)1 - Cable adapter1 - Crimp lug or mechanical lug 1 - Stud 1 - Nut 1 - Plug 1 - Rubber cap1 - Cable sealing kit (if ordered)Silicone lubricant, paper wipes, Iso (Isopropyl Alcohol) wipes and installation instructions.Tools required●●13-mm open-end wrench ●●22-mm socket●●Torque wrench up to 70 Nm●●For mechanical lug (if ordered):●●6-mm Allen hex key for 400 mm 2size ●●8-mm Allex hex key for all other sizesOptional Capacitive T est Point Operating Instructions: Use only voltage indicating instruments specifically designed for test points . Use of conventional voltage sensing devices may provide false “No Voltage” indications .The test point must be dry and free of contaminants when checking for voltage . After indication is taken: clean, dry, and lubricate the test point cap with siliconegrease and assemble to the test point .Figure 2 . T ee connector components (sequential order of installation shown)3DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screenand Outer SheathInstallatIon InstructIons MN650023EN October 2016Cable preparation (1)1. Train the cable to the desired finished position and cutthe cable. (See Figure 3.)ote:N If the cable is visibly bent, straighten it beforeinstalling the tee. A bent cable will result in aloose interface and reduce the dielectric withstand of the interface, especially for large cable sizes. Recommended method for straightening the cable: Heat up the cable to 90 °C by using a heat tape. Keep the tape on for approximately 120 minutes and then remove the heat tape. Keep the cable straight while cooling. It may be helpful to tape a splint to the cable while it is cooling down.Crimp LugChamferMechanical LugChamferFigure 3 . Cable preparation for crimp lug (top) and mechanical lug (bottom)2. Remove the cable jacket for a distance of 315 mm (forcrimp lug only) or 320 mm (for mechanical lug only) from the end of the cable.3. Pull back drain wires. Use vinyl tape to secure wirestemporarily.4. Remove 265 mm (for crimp lug only) or 270 mm (formechanical lug only) of insulation screen. Chamfer the sharp end at a 45° angle.5. Remove “L ” mm of cable insulation.ote:N Determine the value of “L ” from Table 1.6. Apply three layers of vinyl tape of the end of theconductor.7. Remove vinyl tape from the screen wires. Wrap twohalf-lapped layers of sealing tape over cable sheath, then pull down the screen wires. Wrap two layers of sealing tape over screen wires, and cover the end ofcable sheath and screen wires.Figure 4 . Application of tape to conductor and screen wiresT able 1 . Dimension “L ”Conductor Size (mm 2)Crimp Lug Mechanical LugL (mm)L (mm)Centering Sleeve Required955054Yes 120No 150No 1857772No24030040087No 500105116No 630115800-123NoAdapter (2)1. Polish and thoroughly clean the cable insulation, usingsand paper provided if necessary, and Iso wipes.2. Wrap two turns of vinyl tape to serve as marker at aposition of 23 mm from end of insulation screen (seeFigure 5).Figure 5 . Application of tape marker for cable adapter 3. Lubricate exposed cable insulation and adapter insideinterface.4. Slide the cable adapter onto the cable, black end first,until the black end is flush with tape marker. Check the Critical Dimension as shown in Figure 6. Then remove the marker.4DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screen and Outer SheathInstallatIon InstructIons MN650023EN October 2016Figure 6 . Cable adapter: Critical Dimension for crimp lug and mechanical lug5. Wipe off grease from end of cable adapter preparingsurface for application of tape.6. Apply 3-4 layers of semi-conductive tape over theadapter edge and cable screen wires. Then apply 2 layers of sealing tape over the semi-conductive tape and caulk screen wires. Wrap 2-3 half-lapped layers of vinyl tape over the sealing tape.7. Remove the tape from the end of the conductor.Crimp lugs (3)Figure 7 . Crimp lug crimp sequence1. Wire brush conductor (aluminum only). Wipe offaluminum dust. Do not get dust on cable adapter.2. Immediately insert the conductor into the crimpingbarrel as far as it will go. Ensure that the flat of the lug spade is parallel to the face of the bushing.3. Crimp the lug starting at the mark on the barrel of thespade end of the lug as shown in Figure 7. Rotate the crimping tool 90 degrees for each successive crimp.4. Remove any excess inhibitor that may have come outof the crimp lug. File off any sharp projections. Apply a layer of vinyl tape on the barrel.T able 2 . Crimp and Mechanical Lug ChartConductor Size (mm 2)Part Number (DIN Type)Die Size Part Number (Mechanical)Allen Hex Key95CDT630U9522CDT630SB1508 mm120CDT630U12022 150CDT630U15025 185CDT630U18528CDT630SB300240CDT630U24032 300CDT630U30034 400CDT630U40038CDT630SB400 6 mm 500CDT1250U50044CDT1250SB6308 mm630CDT1250U63048 800--CDT1250SB800Mechanical lugs (3)1. Check Table 1 to see if a centering sleeve is required.If required, check the chart supplied with the lug to determine the correct color sleeve for the conductor size being used.2. Wire brush conductor (aluminum only).3. Immediately insert the conductor into the barrel ofthe lug and visually check that the lug is on as far as possible.4. With the appropriate Allen hex key (per Table 2), screwin bolt No. 1 until it breaks off smoothly. Proceed with bolt No. 2 in a similar way. If the lug has more than two (2) bolts, continue with No. 3 (See Figure 8 for tightening sequence).5. Once all the bolts have broken off, smooth any roughedges with a file and install the covering caps. Carefully clean off any filings.Centering sleeveFigure 8 . Mechanical lug tightening sequence5DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screenand Outer SheathInstallatIon InstructIons MN650023EN October 2016Tee body (4)1. Clean the outer surface of the cable adapter and theinterior of the cable entrance of the tee connector with a lint-free cloth. Apply a thin layer of lubricant to both surfaces.2. Push the tee connector body over the cable adapter asfar as it will go. Ensure that the hole in the top of the crimp connector is visible through the interface end ofthe tee.Figure 9 . T ee connector body installationStud (5)1. Tighten the stud to 55 Nm, using a 13-mm open-endwrench.Figure 10 . Installation of stud in bushingTee (6)1. Clean the bushing interface and the tee connectorinterface with an Iso wipe. Wait for cleaning solvent to evaporate. Lubricate both interfaces.2. Push the tee connector onto the bushing. Make surethe stud passes through the hole in spade of lug.ote:N If a companion tee is to be installed, do notproceed any further . Follow the DTB1242 Deadbreak Bolted Companion T ee ConnectorInstallation Instructions MN650024EN .Figure 11 . Installation of nut and deadend plugNut (7)1. Put hex nut on the stud; then tighten the nut to 70 Nmusing a torque wrench and a 22-mm socket.Plug (8)1. Clean the deadend plug and the tee connectorinterfaces with cleaning wipes. Wait for cleaningsolvent to evaporate. Apply a thin layer of lubricant to both surfaces.2. Insert the deadend plug into the tee and engage thethreads of the threaded stud.3. Tighten the deadend plug to 55 Nm using a torquewrench and 22-mm socket.Cap and earth wire (9)1. Clean the interior surface of the rubber cap.2. Place it over the deadend plug and push it until it snapsinto place.3. If not already attached, connect drain wire to thegrounding tab of tee body as shown in Figure 12.4. Connect drain wire on tee body to earthing system.5. Clamp the cable below cable jacket seal.6DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screen and Outer SheathInstallatIon InstructIonsMN650023EN October 2016Figure 12 . Cable clamp and screen wire earthing arrangement7DTS1242 Deadbreak Bolted Tee Connector: Interface C for Single Core Cable with Copper Wire Screen and Outer SheathInstallatIon InstructIons MN650023EN October 2016his page is intentionally left blank.TEaton1000 Eaton Boulevard Cleveland, OH 44122United StatesEaton’s Power Systems Division 2300 Badger Drive Waukesha, WI 53188United States/cooperpowerseries© 2016 EatonAll Rights ReservedPrinted in USAPublication No. MN650023EN Rev 00 October 2016Eaton is a registered trademark.All trademarks are propertyof their respective owners.For Eaton's Cooper Power series productinformationcall 1-877-277-4636 or visit:/cooperpowerseries.。

For Genome DNA AnalysisNot IG C G G C C G C C G C C G G C GTaKaRa Code：D1166A 包 装 量：150 Units附带试剂： 10×H Buffer 500 μl 0.1% BSA 500 μl 0.1% TritonX-100 500 μl 10×Loading Buffer 500 μl纯 度：1） Overdigestion Test：≥15 Units 2） Ligation-Recutting Test： Ligation Effi.：＞95%,Recutting Effi.：100% 3） Genome DNA Digest：≥20 Units 4） pKF3 Cloning Test：＜2%●起在上述在50 μl1） 2） Ligation-Recutting Test：在经过10倍量该酶切出的DNA 片段中，加入 T4 DNA Ligase，使其连接，然后再使用该酶进行切断反应，判断Ligation-Recutting 效率。

3） Genome DNA Digest：在0.5 μg 的Escherichia coli GenomeDNA（包埋于0.5% Agarose Gel 中）中，加入过量的该限制酶，进行长时间（24 小时）酶切反应，然后进行脉冲电泳，确定切出的DNA 片段的电泳谱带不发生变化。

4） pKF3 Enforcement Cloning Test：使用10倍量的该酶，将Enforcement Cloning Vector pKF3 DNA 切开，然后再进行连接后，转化至TH2感受态细胞中，判断该酶切位点受到影响的重组体所占的比率。

●在各种Universal Buffer 中的相对活性：* +0.01% BSA+0.01% TritonX-100 →100%●各种DNA 的切断数： 使用时添加反应液量的1/10，即可停止反应，进行电泳。

IT-180ABS/IT-180ATCHigh Tg, Low CTE, Multifunctional Filled Epoxy Resin and Phenolic-Cured Laminate & PrepregIT-180A is an advanced high Tg (175℃ by DSC) multifunctional filled epoxy with low CTE, high thermal reliability and CAF resistance. It’s design for high layer PCB and can pass 260℃ Lead free assembly and sequential lamination process.Key Features =============================== Advanced High Tg Resin TechnologyIndustrial standard material with high Tg (175℃ by DSC) multifunctional filled epoxy resin and excellent thermal reliability. Lead-Free Assembly CompatibleRoHS compliant and suitable for high thermal reliability needs, and Lead free assemblies with a maximum reflow temperature of 260℃. Friendly Processing and CAF ResistanceFriendly PCB process like high Tg FR4. Users can short the learning curve when using this material.CAF ResistanceLow thermal expansion coefficient (CTE) helps to excellent thermal reliability and CAF resistance providing long-term reliability for industrial boards and automobile application.Available in Variety of ConstructionsAvailable in a various of constructions, copper weights and glass styles, including standard(HTE), RTF and VLP copper foil. ApplicationsMultilayer and High Layer PCB AutomobileBackplanesServers and Networking TelecommunicationsData StorageHeavy Copper ApplicationIndustrial ApprovalUL 94 V-0IPC-4101C Spec / 99/ 101/ 126 RoHS CompliantGlobal AvailabilityITEQ Laminate/ Prepreg : IT-180ATC / IT-180ABS IPC-4101C Spec / 99 / 101 / 126LAMINATE( IT-180ATC)Thickness<0.50 mm[0.0197 in] Thickness≧0.50 mm[0.0197 in] Units Test MethodPropertyTypical Value Spec Typical Value Spec Metric(English) IPC-TM-650 (or as noted)Peel Strength, minimumA. Low profile copper foil and very low profilecopper foil - all copper weights > 17µm[0.669 mil]B. Standard profile copper foil1.After Thermal Stress2.At 125°C [257 F]3.After Process Solutions 0.88 (5.0)1.23 (7.0)1.05 (6.0)1.05 (6.0)0.70 (4.00)0.80 (4.57)0.70 (4.00)0.55 (3.14)0.88 (5.0)1.40 (8.0)1.23 (7.0)1.23 (7.0)0.70 (4.00)1.05 (6.00)0.70 (4.00)0.80 (4.57)N/mm(lb/inch)2.4.82.4.8.22.4.8.3Volume Resistivity, minimumA. C-96/35/90B. After moisture resistanceC. At elevated temperature E-24/125 3.0x1010--5.0x1010106--103--3.0x10101.0x1010--104103MΩ-cm 2.5.17.1Surface Resistivity, minimumA. C-96/35/90B. After moisture resistanceC. At elevated temperature E-24/125 3.0x1010--4.0x1010104--103--3.0x10104.0x1010--104103MΩ 2.5.17.1Moisture Absorption, maximum - 0.12 0.8 % 2.6.2.1 Dielectric Breakdown, minimum - - 60 40 kV 2.5.6 Permittivity (Dk, 50% resin content)(Laminate & Laminated Prepreg)A. 1MHzB. 1GHzC. 2GHzD. 5GHzE. 10GHz 4.44.44.24.14.05.44.44.44.34.14.15.4 -- 2.5.5.92.5.5.13Loss Tangent (Df, 50% resin content)(Laminate & Laminated Prepreg)A. 1MHzB. 1GHzC. 2GHzD. 5GHzE. 10GHz 0.0150.0150.0150.0160.0170.0350.0140.0150.0150.0160.0160.035 -- 2.5.5.92.5.5.13Flexural Strength, minimumA. Length directionB. Cross direction --------580(84,300)450(65,400)415 (60,190)345 (50,140)N/mm2(lb/in2) 2.4.4Arc Resistance, minimum 125 60 125 60 s 2.5.1Thermal Stress 10 s at 288°C [550.4F],minimumA. UnetchedB. Etched PassPassPass VisualPass VisualPassPassPass VisualPass VisualRating 2.4.13.1Electric Strength, minimum(Laminate & Laminated Prepreg) 45 30 -- -- kV/mm 2.5.6.2 Flammability,(Laminate & Laminated Prepreg) V-0 V-0 V-0 V-0 Rating UL94 Glass Transition Temperature(DSC) 175 170 minimum 175 170 minimum ˚C 2.4.25 Decomposition Temperature -- -- 345 340 minimum ˚C 2.4.24.6(5% wt loss) X/Y Axis CTE (40℃ to 125℃) -- -- 10-13 -- PPM/˚C 2.4.24Z-Axis CTEA. Alpha 1B. Alpha 2C. 50 to 260 Degrees C ------------452102.760 maximum300 maximum3.0 maximumPPM/˚CPPM/˚C%2.4.24Thermal ResistanceA. T260B. T288 -------->60>3030 minimum15 minimumMinutesMinutes2.4.24.1CAF Resistance -- -- Pass AABUS Pass/Fail 2.6.25 Comparative Tracking Index(CTI) -- -- 175~250V -- V UL-746 The above data and fabrication guide provide designers and PCB shop for their reference. We believe that these information are accurate, however, the data may vary depend on the test methods and specification used. The actual sales of the product should be according to specification in the agreement between ITEQ and its customer. ITEQ reserves the right to revise its data at any time without notice and maintain the bestinformation available to users.IT-180ABS/IT-180ATCHigh Tg, Low CTE, Multifunctional Filled Epoxy Resin and Phenolic-Cured Laminate & Prepreg---------------------------------------------------------------------------------------------------------------------------------------------- Process Guideline1. Prepreg Handling & Storage(1) Shelf life is at least 3 months when prepreg stored in a cool dry environment (Temperature: <20 ℃ and Humidity: <50%).(2) Prepreg exposed to humidity should be resealed to minimize moisture absorption.(3)Prepreg should be stored in controlled environment for 12 hours prior to use.(4)Prepreg supplied in rolls or panels should be stored horizontally. To avoid damage, no stacking is recommended.2. Laminate Handling & Storage(1) Laminates should be stored in a dry environment(2) Laminate should always be stored flat3. Inner Layer Process(1)First around must be taken to determine suitable parameters (such as dimensional compensation, etc) before mass production.(2) Inner layers should be baked for at least 40 min at 120 ℃ after black or brown oxides treatment.Note: The material temperature is not allowed to >195 ℃ in lamination process if brown oxide treatment is used.4. Lamination Overview(1)Stacks must be prepared in lay-up room to avoid moisture absorption.(2) Recommended pressure ranges should be as follows:Hydraulic/350~400psiVacuum Hydraulic/300~400psi(3) For Lien Chieh press, heating rate is 1.3~1.8℃/min from 80℃ to 140℃, and for Burkle press, the heating rate is 1.5~3.0℃/min from 80℃to 140℃. Cooling rate below 3℃/min is recommended.(4) When the board temperature reaches 180℃ during the pressing process, the lamination process should be kept for at least 60 minutes.5. DrillingDrilling parameters are mainly dependent on the hole size, layer thickness, layer number, copper thickness, and stack height. The following drilling parameters are for reference only. Typical drilling parameters for 0.4~1.0 mm drills are as follows:Spindle speed: 45~105 KRPM Feed rate: 50~150 IPMRetract rate: 500~1000 IPM Max. hit count: <1000 HITSStack height: ≤2pnls(2~6layers), 1pnl(≥8layers) Entry Material: 0.2mm AluminumBack-up Material: 1.5mm Phenolic laminate Drilling Machine: Hitachi ND-6L210EBaking condition: After Drilling: 170 °C /2 hours6. DesmearThe following desmear parameters are for reference only:Horizontal (JETCHEM)Swell : 75℃ for 100 s Mn+7 : 55-65 g/ l at 85℃ for 180sVertical (ROHMHAAS)Swell : 65℃ for 365 s Mn+7 : 65-75 g/ l at 75℃ for 750sNormally, the typical parameters used to desmear FR-4 product may not produce optimum hole topography for IT-180A. One should consult with your chemical supplier to optimize your desmear condition, e.g. desmear two times or adjust other parameters, etc.。