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Agronomic and environmental impacts of phosphorus

Agronomic and environmental impacts of phosphorus
Agronomic and environmental impacts of phosphorus

ORIGINAL ARTICLE

Agronomic and environmental impacts of phosphorus fertilization of low input bahiagrass systems in Florida

Augustine K.Obour ?Maria L.Silveira ?

Joao M.B.Vendramini ?Lynn E.Sollenberger ?

George A.O’Connor ?James W.Jawitz

Received:11May 2010/Accepted:30July 2010/Published online:19August 2010óSpringer Science+Business Media B.V.2010

Abstract Phosphorus management in low input bahiagrass (Paspalum notatum Flugge)systems rep-resents a major challenge of agronomic and environ-mental importance.Concerns over agricultural P transport to surface waters have prompted several revisions in the P fertilizer recommendations for bahiagrass in Florida.This study evaluated the effects of revised P fertilizer recommendations on forage dry matter yield (DMY)and nutritive value and the potential impacts on water quality in bahiagrass pastures growing on a Spodosol.Treatments con-sisted of the factorial combination of three N rates (0,56,and 112kg N ha -1)and four P rates (0,5,10,and 20kg P ha -1),replicated three times and applied annually in May of 2007and 2008.Forage was harvested at 28-d intervals and DMY,tissue P and crude protein concentration,and P uptake were measured.To monitor P leaching potential,suction cup lysimeters were installed at 15-,30-,60-,90-,and

150-cm depths.In 2007,bahiagrass DMY was not affected by P;however,in 2008there was a linear increase in DMY from 6.9to 8.2Mg ha -1as P rate increased.In both years,tissue P increased linearly from 2.1to 2.5g kg -1as P rates increased from 0to 20kg P ha -1.Similarly,P uptake increased from 14kg P ha -1for the control to 19kg P ha -1for the treatment receiving 20kg P ha -1.During the 2-year study,P fertilization had no impact on soil Mehlich-1,water-extractable P,and leachate P concentrations.Leachate P concentrations at the 15-and 30-cm depths varied seasonally (from 0.05to 0.85mg P l -1),with greater spikes occurring during periods of high water table conditions.Current P fertilization recommenda-tion can increase bahiagrass DMY with no adverse impacts on water quality.

Keywords Leaching áPasture fertilization áSpodosol áWater quality

Introduction

Bahiagrass is the most widely planted perennial forage grass in Florida,occupying about 2million ha in the state (Muchovej and Mullahey 2000).Bahiagrass pastures are predominantly grown on Spodosols and are well adapted to the sandy soils of Florida.The grass tolerates low soil fertility conditions,low pH,and intermittent wet conditions and produces reasonable

A.K.Obour áM.L.Silveira (&)áJ.M.

B.Vendramini Range Cattle Research and Education Center,University of Florida,3401Experiment Station,Ona,FL 33865,USA e-mail:mlas@u?.edu

L.E.Sollenberger

Agronomy Department,University of Florida,2185McCarty,Gainesville,FL 32611,USA

G.A.O’Connor áJ.W.Jawitz

Soil and Water Science Department,University

of Florida,106Newell Hall,Gainesville,FL 32611,USA

Nutr Cycl Agroecosyst (2011)89:281–290DOI 10.1007/s10705-010-9393-1

forage yields in droughty conditions.Most forage-based cow-calf(Bos sp.)systems in Florida rely on bahiagrass pasture as the major source of energy and protein for most of the growing season,therefore adequate production of bahiagrass forage is critical for the success of the cow-calf industry in the state.

Approximately80%of cow-calf production in Florida is concentrated in environmentally sensitive areas in Central and South Florida(FDCAS1998), where P transport from agricultural activities repre-sents a serious threat to water quality(Reddy et al. 1999).The primary cow-calf production region in the state,the Lake Okeechobee watershed,faces signif-icant problems associated with nonpoint-source pol-lution of surface waters by agricultural P.Although dairy and beef operations have been suggested as the major contributors of P to Lake Okeechobee watershed(Burgoa et al.1991),potential P losses from low-input cow-calf systems are smaller than other intensive agricultural operations such as con-?ned animal feeding operations and intensive row-crop farming.However,because of the extensive acreage occupied by grazinglands in Florida,P transport from beef ranching operations can poten-tially have major impacts on water quality(Allen 1988).Therefore,effective P-management strategies that balance productivity and environmental protec-tion are required to support the continued success of the cow-calf industry and water quality programs in the state.

In recent years,concerns about the environmental impacts of P fertilization on bahiagrass pastures have prompted several revisions in the University of Florida bahiagrass fertilization recommendations (Hanlon et al.2008).The current P fertilizer recom-mendation for established bahiagrass in Florida is based on both soil and plant tissue P testing (Mylavarapu et al.2007).Phosphorus is recom-mended only when tissue P concentrations are below1.5g kg-1and soil test P levels are very low or low(\15mg kg-1Mehlich-1P).However,there are limited?eld data to validate the revised P fertilization recommendations for established bahia-grass pastures.

The emphasis of most previous studies on bahia-grass P fertilization has been maximizing bahiagrass yield at relatively high levels of fertilizer P.Thus, despite signi?cant literature on bahiagrass response to P fertilization,there is limited information on strategies that balance productivity with environmen-tal implications.Field research is necessary to determine the minimum amount of P required to sustain bahiagrass yield and pasture persistence without adversely affecting water quality.This is particularly important in Florida where the hydrology in combination with environmental conditions and soil characteristics accelerate nutrient transport of agricultural P to surface water.The objectives of the study were to(i)investigate bahiagrass response to reduced P fertilization rates and(ii)evaluate the potential effects of bahiagrass P fertilization on soil-test P concentrations and water quality.

Materials and methods

The experiment was conducted on an established bahiagrass?eld(27°230N,81°560W)on Smyrna sand (sandy,siliceous,hyperthermic Aeric Alaquods). Initial soil characterization of the Ap horizon(to a depth of15cm)showed an average soil pH of5and Mehlich-1extractable P,K,Mg,and Ca of5,40,74, and579mg kg-1,respectively.Bahiagrass was established in1990and had no history of N and P fertilization during the past15years.Plot size was 12.2912.2m,with a3.1-m alley between plots. Treatments were a factorial combination of three N application rates(0,56and112kg N ha-1year-1) and four P rates(0,5,10,and20kg P ha-1year-1) arranged in a completely randomized design with three replications,for a total of36plots.The56and 112kg N ha-1rates correspond to the recommended University of Florida Institute of Food and Agricul-tural Sciences(UF-IFAS)low and medium bahia-grass N options,respectively(Mylavarapu et al. 2007).Phosphorus application rates correspond to0, 0.5-,1-,and2-times the UF-IFAS P fertilizer application rate of10kg P ha-1.Nitrogen was applied as ammonium nitrate and P as potassium phosphate.Each plot also received a basal annual application of47kg K ha-1(as KCl)and5kg ha-1 of micronutrient mix(F503G micromix)containing 24g kg-1of B and Cu,144g kg-1of Fe,60g kg-1 of Mn,0.6g kg-1of Mo,and56g kg-1of Zn.Plots received lime prior to the initiation of the study to raise the soil pH to 5.5.The experiment was conducted for2years(2007and2008),and treat-ments were applied in May of each year.

Forage dry matter yield,crude protein and tissue

P analysis

The plots were harvested at28-d intervals from June to October each year to determine DMY,tissue N and P concentrations,and N and P uptake.During each harvest,two0.9-96.1-m forage strips were har-vested from each plot to a7.5-cm stubble height using a forage harvester.The remaining herbage was mowed with a?ail harvester at the same stubble height and removed from the experimental site. Samples were weighed fresh,and sub-samples weighed and oven dried at60°C for48h for DMY determination.Dried samples were ground to pass a 1-mm mesh screen in a Wiley mill.Cumulative DMY was calculated as the sum of individual harvests for each year.

Tissue N and P were determined using the Kjeldahl digestion procedure(McKenzie and Wallace1954). Brie?y,0.2g of plant material and2g Kjeldahl digestion mixture were digested in5ml concentrated sulfuric acid at365°C for3–4h.Digested samples were diluted to100ml and analyzed on a Seal AQ2 discrete auto analyzer using the Kjeldahl N and P procedures(USEPA1993).Crude protein(CP)con-centration was calculated as percent N multiplied by 6.25(Stewart et al.2007).Nitrogen and P uptake were calculated as the product of tissue N and P concen-tration and DMY for each plot and harvest.

Soil analysis

Prior to treatment application,and at the end of each growing season,?ve composite soil core samples were taken from the Ap(0–15cm),E(16–30cm)and Bh (31–60cm)horizons of each plot.Soil samples were air dried,crushed,and sieved through a2-mm stainless steel screen and analyzed for Mehlich-1P(Mehlich 1953)and WEP(Page et al.1982).Mehlich-1extract-able P concentrations was determined by equilibrating 5g of soil and20ml of Mehlich-1solution (0.0125M H2SO4and0.05M HCl)for5min on a reciprocating shaker and then?ltering through a Whatman#42?lter paper.Phosphorus concentrations were measured colorimetrically on a Seal AQ2discrete analyzer(USEPA1993).Water-extractable P was determined by equilibrating2g of air-dried soil and 20ml of DDI water.The suspension was shaken on an orbital shaker at a rate of200strikes min-1at room temperature for1h.After shaking,the samples were centrifuged at3,2009g for10min,and the superna-tant was?ltered(0.45l m).Phosphorus analysis was performed as described for Mehlich-1P.

Water quality monitoring

Water quality was monitored on plots receiving annual application of56kg N ha-1and0,5,or 10kg P ha-1.The N rate of56kg ha-1represents the UF/IFAS low N option and is use most commonly in cow-calf systems in Florida.The plots were isolated hydrologically by berms and ditches.Due to the?at topography of the landscape,coupled with the sandy nature of the soil,leaching and sub-surface runoff are expected to be the predominant pathways of nutrient losses.The berms and dense ground cover are expected to further reduce the risks of nutrient transport via surface runoff.

Five suction cup lysimeters(referred to as lysime-ters herein)were installed in the center of each plot at 15-,30-,60-,90-,and150-cm depths.The15-and 30-cm lysimeters were located above the spodic horizon,whereas lysimeters at60-,90-,and150-cm were below the spodic horizon.Leachate samples were collected after rainfall events[10mm.A total of12leachate samples were collected per year in 2007and2008.Samples were collected within 2–24h(depending on the soil moisture conditions) using a hand vacuum pump(*60kPa)and stored at 4°C until analysis.Lechate samples were?ltered through a0.45-l m pore size?lter and analyzed for ortho-P concentration using a Seal AQ2discrete auto analyzer(USEPA1993).

Statistical analysis

Statistical analyses were performed using Proc Mixed (SAS1999).Nitrogen and P rates and year were considered?xed effects,with replicates and their interactions considered as random effects.Year was considered a?xed effect because of the potential for carryover effects of treatments from Year1to2.Year was included in the model as a subplot treatment in a split-plot arrangement,with the treatment combina-tions being the main plots.The PDIFF test of the LSMEANS procedure and single degree of freedom orthogonal contrasts were used to compare means. Treatments and their interactions were considered

signi?cant when F -test P values were \0.05.Inter-actions not discussed in the results and discussion section were not signi?cant (P [0.05).The means reported are least squares means.

Results and discussion Climatological data

Total rainfall recorded in 2007and 2008was below the 67-year average (Table 1).Rainfall in 2008was greater than 2007,especially during the months of May through August,which correspond to the peak of forage production in South Florida.Minimum and maximum temperatures were similar during the 2-year study.

Cumulative bahiagrass dry matter yield

There was signi?cant (P =0.03)interaction between P application and year for cumulative bahiagrass DMY (Table 2).Although P application showed no effects on forage DMY in 2007,bahiagrass increased linearly to increasing P application in https://www.doczj.com/doc/fb8509237.html,-pared to the zero P control,in 2008bahiagrass DMY

increased *4,10,and 19%when P was applied at rates of 5,10,and 20kg P ha -1,respectively.Results observed in 2008are consistent with those reported by Rechcigl et al.(1992)who observed a 25%yield

Table 1Monthly average maximum and minimum temperatures and total monthly rainfall at the range cattle research and education center,Ona,FL in 2007and 2008Month

Temperature Rainfall

20072008

Min (°C)

Max (°C)Min (°C)Max (°C)2007(mm)2008(mm)67-year

average a (mm)Jan.13231422382355Feb.12221623163967Mar.17251724515780Apr.1828202741863May 22302231107096June 23302531203249221July 26322431152195213Aug.26322631208250210Sept.25312530169141187Oct.24291828524279Nov.1926162521950Dec.15241422523151Total

992

1,124

1,372

a

Source :Sellers 2006

Table 2Cumulative bahiagrass dry matter yield (DMY),tis-sue P concentration,and P uptake as affected by P application rates and on bahiagrass swards P rate (kg ha -1)

Cumulative DMY a Tissue P b (g kg -1)

P uptake b (kg ha -1)

2007(Mg ha -1

)2008(Mg ha -1)0 6.5 6.9 2.113.957.07.2 2.315.710 6.67.6 2.417.320 6.88.2 2.518.9SE 0.30.30.060.7Polynomial contrast

NS c

L**

L***

L***

**P B 0.01;***P B 0.0001

a

Cumulative DMY was calculated as the sum of individual harvests for each year.Data are means across three N rates and three replicates (n =9)

b

Data are means across three N rates,2years,and three replicates (n =18)

c

NS not signi?cant,L linear

increase in bahiagrass plots grown on a Spodosol and receiving24kg P ha-1compared to control(zero P) treatments.In that study,N application rates were similar to our study(120kg N ha-1),and the bahi-agrass yield response was linear for P rates up to 24kg ha-1.However,Rhoads et al.(1997)showed that bahiagrass grown on an Ultisol in North Florida receiving336kg N ha-1can respond to P applica-tion rates as high as84kg P ha-1.

There was a linear response of bahiagrass DMY to N application rates in both years;however,the interaction‘N9P’was not signi?cant.Cumulative DMY in2007ranged from4.9to8.4Mg ha-1for the 0and112kg N ha-1treatments,respectively, whereas in2008DMY was 5.1and9.9Mg ha-1 for the same treatments.More rainfall in2008likely caused greater DMY in that year,and it also may have increased response to P fertilizer,resulting in a linear DMY response to P applications.

Our results showed that bahiagrass response to P application can be variable depending on year and environmental conditions.This trend may also explain the apparent discrepancies in the literature relative to the effects of P fertilization on bahiagrass pastures in South Florida.For instance,whereas Rechcigl et al.(1995)showed that P application had no effect on bahiagrass DMY,other studies by the same researchers(Rechcigl et al.1992;Rechcigl and Bottcher1995)using similar P application rates, showed that bahiagrass DMY increased quadratically to P application with maximum yield obtained at 24kg P ha-1.Ibrikci et al.(1992)showed that bahiagrass response to P may also depend on the application rate.Bahiagrass responded to P rates of 17kg P ha-1,beyond which there was no response to additional P(up to68kg P ha-1).The poor correla-tion between P application rates and bahiagrass DMY (R2=0.13–0.23)observed by Rechcigl and Bottcher (1995),indicates that other factors such as climate conditions may have affected bahiagrass response to fertilizer application.As in our study,Ibrikci et al. (1999)observed no response to P in Year1of a 2-year study,but P application increased bahiagrass DMY in Year2.Although the authors did not explain the lack of response in Year1,they showed that P application increased root length density which may have enhanced P uptake over time and,consequently, increased DMY in Year2.Tissue P concentration and P uptake

Tissue P concentration was affected by P application rate(P=0.002)and year(P\0.0001).Tissue P concentration increased linearly in response to P application rates from0to20kg P ha-1(Table2). Tissue P varied from2.1(no P added)to2.5g kg-1 (20kg P ha-1).These values were well above the proposed critical limit of1.5g kg-1(Silveira et al. 2007),which explains the lack of DMY response to P in2007and the relatively small response observed in 2008.The linear increase in tissue P concentration as a function of P application rate suggests luxury P consumption by bahiagrass.These tissue P concen-trations,despite low Mehlich-I soil P in the Ap horizon,support the suggestion that bahiagrass growing on Spodosols accesses P from below the Ap(Ibrikci et al.1994).

Tissue P was greater in2007(2.5g kg-1)than in 2008(2.2g kg-1).The interaction between P rates and year was not signi?cant(P=0.3).Tissue P concentration in2007were2.3,2.4,and2.6g kg-1 for the treatments receiving0,5,10,and 20kg P ha-1,respectively;whereas the same treat-ments showed tissue P concentrations of1.9,2.1,2.3, and2.4g kg-1in2008.Greater tissue P concentration in2007was likely due to the greater yields obtained in 2008which resulted in greater dilution of tissue P. Similar results were also reported by Vendramini et al. (1999)who observed a linear decrease in tissue P concentration as a function of bahiagrass yield.Unlike results reported by Silveira et al.(2010)from a greenhouse study,N rates had no effect(P=0.07)on tissue P concentrations.Differences were likely due to the much smaller N application rates used in our study (0to112kg N ha-1)than by Silveira et al.(2010)(up to300kg N ha-1).

Tissue P concentrations observed in this study are consistent with previous research conducted on sim-ilar soils in South Florida.Adjei et al.(2006)observed tissue P concentrations ranging from1.5to3.0g kg-1 in bahiagrass plots receiving0to20kg P ha-1 harvest-1.Rechcigl et al.(1992)reported tissue P concentrations ranging from1.6g kg-1for control unfertilized plots to3.3g kg-1for treatments receiv-ing48kg P ha-1.

Bahiagrass P uptake responded linearly to P appli-cation rates(Table2).Increasing P application rates

from0to20kg ha-1increased bahiagrass P uptake by *36%.Similarly,N application increased bahiagrass P uptake linearly(P\0.0001).On average,N appli-cations of0,56,and112kg ha-1resulted in P uptakes of12,17,and20kg P ha-1.Newman et al.(2009a,b) studying bahiagrass P uptake growing on a Spodosol also reported that N fertilization enhanced bahiagrass P uptake.These authors reported bahiagrass P uptake of 13–32kg P ha-1in plots receiving N application rates of*250to600kg N ha-1year-1.Since DMY reported by Newman et al.(2009a,b)were similar to ours,greater P uptake in those studies compared to our study was likely due to the differences in initial soil P concentrations(Mehlich-1P concentrations in the Ap horizon=29mg kg-1)and N fertilization regimens. Crude protein concentration and N uptake

Crude protein concentration increased linearly with increasing N application but was unaffected by P application(P=0.9).Adjei et al.(2000)and Obour et al.(2009)reported similar lack of response of CP to P application.Averaged across P rates and year,CP concentration varied from92g kg-1for the control to 99for the56N ha-1treatment,and105g kg-1for the treatment that received112kg N ha-1.These CP values are consistent with values reported by others for warm-season grasses in Florida(Adjei et al.2000; Muchovej and Mullahey2000;Obour et al.2009).

There was a year effect on bahiagrass CP concen-tration(P\0.0001),with greater average CP in2007 (106g kg-1)than in2008(87g kg-1).Differences were likely due to variation in environmental condi-tions and the lower bahiagrass DMY in2007.Similar results were also observed by Johnson et al.(2001), who reported a7%reduction in bahiagrass CP concentrations during the months of greater forage production.Sumner et al.(1991)observed a14% decrease in bahiagrass CP concentration in response to greater forage mass production.

Bahiagrass N uptake was affected by N rate (P\0.0001)and year(P=0.008).Bahiagrass N uptake varied from74kg N ha-1(control)to151kg N ha-1for the treatments receiving112kg N ha-1. Nitrogen uptake was greater in2007(116kg N ha-1) than in2008(108kg N ha-1).This was due to the greater tissue N concentrations in2007.Phosphorus addition had no effect on bahiagrass N uptake (P=0.2).Soil phosphorus concentrations

Phosphorus applications to bahiagrass showed no effect on Mehlich-1soil P concentrations in the Ap, E,and Bh horizons.Mehlich-1soil P concentrations in the Ap horizon ranged from4.2mg kg-1(zero P control)to 5.5mg kg-1P for the20kg P ha-1 treatment.Similarly,Mehlich-1P in the Bh horizons was40mg kg-1for the zero P control and46mg kg-1 for the20kg P ha-1treatment.Although soil-test P was not affected by P application rate,there was a year effect on soil-test P concentrations(Table3). After2years of P application,Mehlich-1soil P concentration in the Ap horizon were lower than the initial conditions and were considered very low (\10mg kg-1),according to the current soil test P interpretations for agronomic crops in Florida(My-lavarapu et al.2007).Therefore,based on soil test results alone,it was expected that bahiagrass would respond to P application.Similarly,Mehlich-1P concentrations in the E and Bh horizons found at the end of2007and2008growing season were either smaller or similar to the initial conditions(Table3). This result suggests that P application at rates similar to P removal will likely have no impacts on soil-test P concentrations.

Regardless of treatment,P concentration in the Bh horizon was always greater than in the Ap and E horizons(Table3).The substantial amount of P held in the Bh horizon may be plant available(Ibrikci et al.1994)and likely contributed to bahiagrass P uptake,masking treatment effects.

There was no effect of P application on WEP concentration(P=0.75).Water-extractable P con-centration averaged across treatments after2-year of P fertilization was3.6,0.4,and2.8mg kg-1for the Ap,E,and Bh horizons,respectively.Numerous studies have shown that WEP is a reliable indicator of P loss potential(Kleinman et al.2002;Ehlert et al. 2003).Results from the current study indicate that addition of relatively low rates of P fertilizer for 2years did not increase the risks associated with P transport,even when P was applied up to twofold the recommended rate(20kg P ha-1).Extensive bahi-agass roots,adequate ground cover,and removal of harvested forage(i.e.,no grazing and associated P return in excreta)likely increased fertilizer utilization ef?ciency and removal from the sward and mini-mized the potential for soil P accumulation.

Leachate phosphorus concentration

There was a year 9P rate 9sampling depth inter-action for leachate P concentration (Table 4).In 2007,leachate P concentration measured in the lysimeters placed at 15cm were not different between the control and the 10kg P ha -1treatments,but concentration for the 10kg P ha -1rate was greater than the treatments receiving 5kg P ha -1(Table 4).For the lysimeters at 30-cm depth,the greatest P concentration of 0.9mg l -1was recorded for the control treatments.In 2008,increased leachate P concentrations at 15-cm depth were observed in the treatments receiving 10kg P ha -1.However,leach-ate P at the 30-cm depth was similar for the control and the treatments receiving 10kg P ha -1,which suggests that limited P leaching from the upper soil layers occurred.Regardless of the P application rate,leachate P concentrations found in lysimeters at 60-,90-,and 150-cm depths were similar in both years.Thus,P application had no effect on leachate P concentration below the spodic horizon.In general,leachate P concentrations in the lysimeters below the spodic horizon ([60cm)were less than in the lysimeters installed at upper depths.This trend suggests there was limited P movement below the Bh horizon during the 2-year study.These results are also consistent with the Mehlich-1and WEP soil data that showed no effect of P fertilization on soil P concentration in the Bh horizon (Table 3).The high variability associated with leachate P in both years (Table 4)re?ects the seasonal ?uctuation in P losses by leaching.It is likely that high water table conditions experienced during the summer months increased P release from the Bh horizon and promoted subsequent transport of P to the surface horizons.Toor et al.(2004)also observed signi?cant variations in leachate P as a function of the season.There was seasonal variation in leachate P during the 2-year study (Fig.1)but the interaction ‘sea-son 9treatment’was not signi?cant (P =0.3).In 2007,leachate P concentrations in all ?ve lysimeters were similar during the ?rst four sampling events (Fig.1a).However,after 4July,there was a gradual increase in leachate P concentration measured in the upper two lysimeters (15and 30cm)than in the lysimeters below the spodic horizon.In 2007,there was a large spike in leachate P concentration in the 15-and 30-cm lysimeters in September and October

T a b l e 3M e h l i c h -1s o i l P c o n c e n t r a t i o n s a t t h e v a r i o u s d e p t h s a s a f f e c t e d b y y e a r

A p h o r i z o n (0–15c m )

E h o r i z o n (16–30c m )

B h h o r i z o n (31–60c m )

I n i t i a l a

(m g k g -1)

2007(m g k g -1)

2008(m g k g -1)S E P v a l u e b I n i t i a l (m g k g -1)2007(m g k g -1)2008(m g k g -1)S E P v a l u e I n i t i a l (m g k g -1)

2007(m g k g -1)2008(m g k g -1)S E P v a l u e

4.0b

6.3a 3.0c 0.4\0.00012.3a 0.9b 1.0b 0.30.000546a

32b 51a

4.20.0001

D a t a a r e m e a n s a c r o s s t h r e e N r a t e s ,f o u r P r a t e s ,a n d t h r e e r e p l i c a t e s (n =36)

a

I n i t i a l s o i l t e s t P r e f e r s t o s o i l P c o n c e n t r a t i o n s i n s a m p l e s c o l l e c t e d p r i o r t o P a p p l i c a t i o n .2007a n d 2008r e f e r t o s a m p l e s c o l l e c t e d a t t h e e n d o f e a c h g r o w i n g s e a s o n (N o v e m b e r o f e a c h y e a r )

b

M e a n s w i t h i n a s o i l d e p t h f o l l o w e d b y t h e s a m e l e t t e r a r e n o t d i f f e r e n t u s i n g t h e L S M E A N S /P D I F F p r o c e d u r e (P [0.05)

Table4Leachate P concentration at the various depths as affected by year and P application rate to bahiagrass swards

20072008

P rate(kg ha-1)

Depth(cm)05100510 Leachate P(mg L-1)

150.4ab a0.07b0.5a0.2b0.09b0.5a 300.9a0.05c0.3b0.3a0.10b0.4a 600.01a0.07a0.009a0.005a0.03a0.03a 900.1a0.02a0.01a0.009a0.03a0.11a 1500.03a0.008a0.006a0.009a0.01a0.02a SE0.20.1

Data are means across12sampling events for each year

a Means within a soil depth and year followed by the same letter are not different using the LSMEANS/PDIFF procedure(P[0.05)

(Fig.1a).For instance,leachate P concentration in the15-cm lysimeter increased from0.03mg l-1on4 June to0.76mg l-1in September,representing a 25-fold increase in P concentration compared to the initial sampling period.A similar trend was observed in2008,with the greatest spike in leachate P concentrations in the upper lysimeters occurring in August and September(Fig.1b).

In both years,the period of large spikes in soil solution P concentration in the15-and30-cm lysimeters coincided with periods of high rainfall and high water table conditions at the experimental site. The high water table might have contributed to P ?uxes from the spodic horizon into the surface layers, thus increasing P concentration in the lysimeters above the spodic layer.The?uctuating water table conditions experienced in Florida may affect the redox conditions in the soil and P release from the spodic,hence contributing signi?cantly to P?uxes from the spodic layer(Pant et al.2002).Further studies are needed to evaluate the in?uence of ?uctuating water table on P bioavailability to bahi-agrass pastures growing on Spodosols in Florida and potential losses of P to the environment. Summary and conclusions

Treatments spanning a range of low levels of P fertilization were targeted in this study because of the critical need to minimize P loss to surface water while maintaining vigorous bahiagrass stands.Across the range of N rates most often used on bahiagrass swards in Florida,increasing P application from0to 20kg ha-1had no effect on DMY in1year and increased DMY by19%in the second year.There was no effect of P application on any soil P response. Leachate P was generally not affected by P applica-tion.The only exception occurred in2008where leachate P concentration at15-cm depth was greater for the10kg P ha-1compared to the other treat-ments.However,leachate P at the30-cm depth was either lower or similar for the10kg P ha-1com-pared to the control in2007and2008,respectively. Results suggested that limited P leaching occurred at depths[30cm.

Additional work with low rates of P is warranted because this study covered a relatively short time scale and because these data are from harvested bahiagrass swards instead of grazed pastures.Results also suggest that further studies are needed to investigate the impact of?uctuating water table on P availability and loss potential.

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常用英文缩写大全(全)

企业各职位英文缩写: GM(General Manager)总经理 VP(Vice President)副总裁 FVP(First Vice President)第一副总裁 AVP(Assistant Vice President)副总裁助理 CEO(Chief Executive Officer)首席执行官,类似总经理、总裁,是企业的法人代表。 COO(Chief Operations Officer)首席运营官,类似常务总经理 CFO(Chief Financial Officer)首席财务官,类似财务总经理 CIO(Chief Information Officer)首席信息官,主管企业信息的收集和发布CTO(Chief technology officer)首席技术官类似总工程师 HRD(Human Resource Director)人力资源总监 OD(Operations Director)运营总监 MD(Marketing Director)市场总监 OM(Operations Manager)运作经理 PM(Production Manager)生产经理 (Product Manager)产品经理 其他: CAO: Art 艺术总监 CBO: Business 商务总监 CCO: Content 内容总监 CDO: Development 开发总监 CGO: Gonverment 政府关系 CHO: Human resource 人事总监 CJO: Jet 把营运指标都加一个或多个零使公司市值像火箭般上升的人 CKO: Knowledge 知识总监 CLO: Labour 工会主席 CMO: Marketing 市场总监 CNO: Negotiation 首席谈判代表CPO: Public relation 公关总监 CQO: Quality control 质控总监 CRO: Research 研究总监 CSO: Sales 销售总监 CUO: User 客户总监 CVO: Valuation 评估总监 CWO: Women 妇联主席 CXO: 什么都可以管的不管部部长 CYO: Yes 什么都点头的老好人 CZO: 现在排最后,等待接班的太子 常用聊天英语缩写

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DARPA :国防高级研究计划局 ARPARNET(Internet) :阿帕网 ICCC :国际计算机通信会议 CCITT :国际电报电话咨询委员会 SNA :系统网络体系结构(IBM) DNA :数字网络体系结构(DEC) CSMA/CD :载波监听多路访问/冲突检测(Xerox) NGI :下一代INTERNET Internet2 :第二代INTERNET TCP/IP SNA SPX/IPX AppleT alk :网络协议 NII :国家信息基础设施(信息高速公路) GII :全球信息基础设施 MIPS :PC的处理能力 Petabit :10^15BIT/S Cu芯片: :铜 OC48 :光缆通信 SDH :同步数字复用 WDH :波分复用 ADSL :不对称数字用户服务线 HFE/HFC:结构和Cable-modem 机顶盒 PCS :便携式智能终端 CODEC :编码解码器 ASK(amplitude shift keying) :幅移键控法 FSK(frequency shift keying) :频移键控法 PSK(phase shift keying) :相移键控法 NRZ (Non return to zero) :不归零制 PCM(pulse code modulation) :脉冲代码调制nonlinear encoding :非线性编程 FDM :频分多路复用 TDM :时分多路复用 STDM :统计时分多路复用 DS0 :64kb/s DS1 :24DS0 DS1C :48DS0 DS2 :96DS0 DS3 :762DS0 DS4 :4032DS0 CSU(channel service unit) :信道服务部件SONET/SDH :同步光纤网络接口 LRC :纵向冗余校验 CRC :循环冗余校验 ARQ :自动重发请求 ACK :确认 NAK :不确认

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招呼篇 GTSY:Glad To See You高兴认识你 PMJI:Pardon My Jumping In =PMFJI:Pardon Me For Jumping In 败势,加入你们的谈话 WB:Welcome Back 欢迎回来 LTNS:Long Time No See 好久不见 笑篇 BEG:Big Evil Grin (非常)邪恶的笑 C&G:Chuckle And Grin 喀喀笑 GMBO:Giggling My Butt Off 笑掉我的屁屁 BWL:Bursting With Laughter 笑掉不行 CSG:Chuckle Snicker Grin 嘿嘿窃笑 KMA:Kiss My A$$ =MKB:Kiss My Butt 亲我的屁屁 LMAO:Laughing My A$$ Of =LMBO:Laughing My Butt Off =LMHO:Laughing My Head Off 笑死我了 LOL:Laughing Out Loud 放声笑 LSHMBB:Laughing So Hard My Belly Is Bouncing =LSHMBH:Laughing So Hard My Belly Hurts 笑到我肚子痛 告知篇 AFK:Away From Keyboard 离开键盘 BBL:Be Back Later =BBS:Be Back Soon =BRB:Be Right Back 稍待回来 CNP:Continue In Next Post 请看下一个留言 FYI:For Your Information 只给你知道 OIC:Oh,I See 喔,瞭 PS:Post Script 附注 QSL:Reply 回答 RTF:Read The FAQ 请看常见问题 AKA:Also Known As 又名为 FAQ:Frequently Asked Question 最常被问的问题 IC:I See 瞭 IGP:I Gotta Pee 我要去尿尿 POOF:I Have Left Chat 我已经离开聊天室啰 PM:Private Massage 私下寄消息。在聊天室常见的功能,你可以单独对有兴趣的人私下聊

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duno=don't know u=you ur=your kinda=kind of sorta=sort of 2=two或to 4=for shoulda=should have congrat=congratulation thx=thanks X'mas=Christmas wat=what biz=business ad=advertisement ft.=featuring abt=about pls=please rgds=regards ---问好--- 1,hiho=hola=yo=hi=hey=hellow=你好,大家好 2,wuz up=sup=what's up=(原意:怎么样你?/有什么事儿嘛?)也可作为问好用(当然是比较熟的两个人之间的问候),回答时有事说事,没事用"nothing/nothin much/not much/nm等回答就可以。 ---再见--- 1,cya=cu=see ya=see you=再见 2,laterz=later=cya later=see ya later=see you later=再见 3,gn=gn8=gnight=good night=晚安 4,nn=nite=晚安 说明:一般第一个人常说gnight/gn8,然后第二个人用nite,后面的用nn什么的都可以了。不要问我为什么,约定俗成而已。 ---惊叹赞扬--- 1,OMG=oh my god=我的天;**! 2,OMFG=oh my f ucking god=我的老天;**靠; 3,wtf=what the f uck=怎么会事!?;*!; 4,n1=nice 1=nice one=漂亮 5, pwnz=ownz=牛比!(例句:pwnz demo!;lefuzee ownz all the others!) 6,rullz=强!(例句:lefuzee rullz!;you guyz rull!!!) 7,you rock!=你牛比!(口语中常用,irc中偶尔能看到。) ---笑--- 1,lol=laughing out loud /laugh out loud=大笑 2,lmao=laughing my ass off=笑的屁股尿流 3,rofl=roll on floor laughing=笑翻天了 排序:hehe

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