An Intelligent People-Flow Counting Method for Passing through a Gate 阅读小结 一、文章思路： 使用一个摄像机和基于面积和颜色分析的方法，对通过门的行人采用分两阶段计数策略：1.使用行人面积初步估算行人数量；2.使用行人的颜色信息进行跟踪并精确初步的估算数量。其中设备系统安装图和算法流程图如下：
二、算法叙述 1、计数算法描述： 首先，设定两条辅助线之间的区域为监测区域，分离前背景，获取接触两条辅助线的行人团块。使用基于面积的计算方法初步估计行人的数量，采用HIS颜色模型标识这些行人团块并且进行跟踪，颜色提取能应对出现行人团块在监测区域内的分离或合并，在接触另一条辅助线时再次精确初步估算的数值。 2、基于面积的人数计算 N p 记为单个人图像的典型面积，指的是最小值。多人（N个）团块的面积由于重叠所以小于N* N p。规则如下： 其中，PN PI 为行人模块总人数，N PI为行人模块的面积。 3、HIS直方图分析 在各种颜色模型中，使用HIS颜色模型描述人非常合适。因此文章采用HIS颜色模型色度向量： 亮度向量： 首选使用色度向量，当饱和度接近0时，使用亮度向量替代色度向量。 k越大匹配耗时越小但误差也越大，k越小匹配精度越高但匹配时间也越大，因此需要选择合适的值。文章色度取k=10，亮度取k=16。 4、行人跟踪： 对各行人模块划定边框，在下一帧跟踪时，如果前后帧行人模块边框重叠且仅是两块则认为是同一行人模块，否则才使用先前建立的HIS颜色向量进行匹配跟踪。
5、合并和分裂处理： 合并：如果前帧的两个行人模块在后帧中合并为一更大（指面积）的行人模块，前后帧模块有重叠则认为是合并情况出现，但对于没有重叠情况只能通过前面的颜色向 量进行匹配跟踪，如果匹配失败，那就很难处理而丢弃匹配跟踪。 分裂：跟合并处理方法差不多道理。 三、文章存在的优点与缺点： 1、优点： 1）算法简单，具有实时效果，准确率高，在单人和正常合并分裂情况下准确率达到100%。2）补充了前人对合并分裂情况的没有考虑的处理。 3）颜色向量的设计和选用 2、缺点： 1）在合并分裂的时候，如果出现前后帧行人模块边框没有重叠且颜色向量在匹配中失效则只能丢弃跟踪，在多个行人模块颜色向量相近是匹配失效的原因之一。因此在行人衣着相近算法误差不增多。 2）在非正常的合并和分裂存在的情况，准确率明显下降。
键盘钢琴谱大全（键盘钢琴曲谱、键盘钢琴琴谱、键盘钢琴乐谱、键盘 钢琴卡农、键盘钢琴谱子） 日期：2011-08-29作者：korey 键盘钢琴是一款非常经典的在线弹钢琴小游戏。但是咱们玩家很多都不会弹钢琴，如果没有键盘钢琴谱的话，咱们只能乱弹一通。 所以，小编YY收集了很多键盘钢琴谱，堪称键盘钢琴谱大全。其中包括键盘钢琴曲谱、键盘钢琴琴谱、键盘钢琴乐谱，当然，更少不了我们最爱的键盘钢琴卡农这个谱子咯！对照着键盘钢琴谱，咱们弹起来吧！ 《卡农》和炫版(括号是一起按） H-JLO E-ILN F-HJM C-GIL D-FHK A-EHJ D-FHK E-GIL (HQ)-JLO (EP)-GIL (OF)-HJM (NC)-GJL (MD)-FHK (LA)-CEH (MD)-FHK (NE)-GIL ONOH (G B )LIJ (CH)ONM (CN)QST (DR)QPR (QA)PON (DM)LKJ (EI)KJI (EH)IJK (EG)ILK (FJ)MLK (CL)KJI (DH)FMN (AO)NML (DK)JIM LML (SJ)QRS-QR(SE)LMNOPQR (FQ)OPQ-JK(GL)MLKLJKL (FK)MLK-JI(JE)IHIJKLM (FK)MLM-NO(GL)MNOPQRS (SI)QRS-QR(SE)LMNOPQR (FQ)OPQ-JK(GL)MLKLJKL (FK)MLK-JI(EJ)IHIJKLM
(FK)MLM-NO(GL)MNOPQRS (HQ)-JLO (EP)-GIL (OF)-HJM (NC)-GJL (MD)-FHK (LA)-CEH (MD)-FHK (NE)-GILNQOQPO 《卡农单音版》 SQR SQR SLMN OPQR QOP QOP QJKL MNON MON MLK LKJK LMNO MON ONO NLMN OPQR SQR SQR SLMN OPQR QOP QOP QJKL MNON MON MLK LKJK LONO MON ONO NLMN OPQRS 《月亮代表我的心》 LOQSONQS STUVTS QPOOO QPOOO PQPOMPQP LOQSONQS STUVTS QPOOO QPOOO PQPMNOPO QSQPOSN MNMNMLQ SQPOSN MNOOOPQP LOQSONQS STUVTS QPOOO QPOOO PQPMNOPO 《生日快乐》 EEFEHG EEFEIH EELJHGF KKJHIH 《世上只有妈妈好》 QPNPSQPQ NPQPNMLJPNM MNPPQNML
广东开心版三年级下册《Unit 8 Counting》2018年单元测试卷一、填空题 1．（8分）用am ，is ，are填空 （1）I young． （2）You beautiful． （3）Jenny strong． （4）He happy． （5）We tired． （6）They cold． （7）you happy ？Yes，I． （8）he happy ？Yes，he． 二、写出下列单词 2．（1分）十三十四十五十六十七十八十九二十 多少桔子桃子三明治樱桃草莓 三、句型转换 3．（1分）There are eleven oranges 否定句： 一般疑问句： 4．（1分）There is one sandwich． 否定句： 一般疑问句： 5．（1分）There is an elephant （变一般问句） elephant ？ 6．（1分）There are fifteen cherries （就划线部分提问） are there ？ 7．（1分）There are thirteen peaches （就划线部分提问） are there ？
广东开心版三年级下册《Unit 8 Counting》2018年单元 测试卷 参考答案 一、填空题 1．am；are；is；is；are；are；Are；am；Is；is； 二、写出下列单词 2．thirteen；fourteen；fifteen；sixteen；seventeen；eighteen；nineteen；twenty；how many；orange；peach；sandwich；cherry；strawberry； 三、句型转换 3．There aren't eleven oranges．；Are there eleven oranges？；4．There isn't one sandwich；Is there one sandwich？；5．Is there an；6．How many cherries；7．How many peaches；
26个字母，26个钢琴键，可以用a-z来弹，也可以用鼠标，呵呵，大家可以来试试呀！ <月亮代表我的心> LOQSONQS STUVTS QPOOO QPOOO PQPOMPQP LOQSONQS STUVTS QPOOO QPOOO PQPMNOPO QSQPOSN MNMNMLQ SQPOSN MNOOOPQP LOQSONQS STUVTS QPOOO QPOOO PQPMNOPO 《童话》 LONOL LONOL LONO OOMML LONOL LQPPO LONOM MMOTS PPRRQQ QQNPOONO ONOR LSRQP PPRRQQ QQVUTUV VPOT TTSSS LSRQQRQ QRQ RQPO OQST TTSPPRQ OQST TTSPPRQRQPO PQMMOONO 《安静》 QQQQPONPPPO LQPOOOLQPOOP QQQQPONPPPO LQPOOOLQPOOPPQR RRRRQPOOOPP LSSSRQPPPQQ MRQRQPOONOL QRQRQPOPS LQRSRQSLQRSRQS LQRSRQOPPPQO SSOONOOSSOONOO RRQQPPORRQQPPO LQRSRQSLQRSRQS LQRSRQOPPPQO SSOONOOSSOONOO RRQQPPOORQPOMOO 《好好恋爱》 JKLLLLLKJIIL NNONOOOPQNNL HMMMJMOLLLLJH KKKJKLMMMOML JKJJKLLLLJIHHO NMLML JHHMMLLLKJ OOOONOPP LRQLLRPLRQRQRQPO ONMMQMQMLLQLQL RQRQOP LRQLLRSPLRQQQRQPQ ONMMQMQMLLQLQPOM OPNMNMNQPOO 《痴心绝对》 OPQQQRQPPOPPSP ONOOOQQOOMNNQN MLMMMRRQSO MLMMMRROOMP OOPQQQRQPPOPPSP ONOOOQQOOMNNQN MLMMRRQSO MLMMMRROOONO 《会呼吸的痛》 STVTXXTW WWVUVWXWSV VUTUVRRRVVWVSSS SYXWXX STVTXXTW WWVUVWXWSVV VUTUVRRRVVWVSSS STUVVUVV
HONOOLLLLKKJ HONOOLLLLPPO H ONONMHNMNML HMLMLKKJKLMM L HONOOLLLLKKJ HONOOLLLLPP O HONONM HNMNML HMLMLKKJK LMML HLHKJ HHLHKJH 《蜗牛》 Flash键盘钢琴谱 J JJKJIJIH HHK KIHHIJ HHL LMLKJK GH EEJ JKJIJH EEH HEHIJKL HONN N ONMMNLHKLM MNOPQL EFLK KLK JIHI EEH HEHIJKL HONN NONMMNL HKLM MNOPQL EFLK KJIHHGH 《123木头人》 Flash键盘钢琴谱LKJJJ III JIHIJO NML MKJKJK IJKONL MLL LKJJJ ILN MNONM J LMK KPOL IJKKJKL J I H 《神秘园》 Flash键盘钢琴谱 jmno no opnmn nomlm lj jmno no opnmn nomlm lm nost tusrs noqr rsqpq nost tusrs strqr rsqpq jmno no opnmn nomlm lj jmno no opnmn nomlm lm
《轨迹》 Flash键盘钢琴谱 qeee reee weewqe qwee eweq hqewqw qee e reee weewqte qwee eweq hqewqw qjqjw q jwqjq q ttqqt qgqrewe ee rewqj wewq qtr ewqwet g r eee ereww weqq ett qttrr ewreq qq wEwqw gr eee ereww weqq ett qttrr e wreq qerw jq 《无赖》 Flash键盘钢琴谱HIJJIJKJIGE FGHHIJJMLJL JLMMMLK JKLLKJ JKKLKHI HIJJIJKJIGE FGHHI JJMLJL JLMMMLK JKLLKJ JKJHIH H HEFGHHIJHE EDDFEDE EFFGJIIHGH HHFEFG EEEJIHHIJHL JJLMMNOLJ JJ LMMMKMNMLKLJ JKJKLMLL EJIHH IJHL JJLMMNONJ JJLMMMKMNMLJI H JKJKLMLLJIHH 《海阔天空》 Flash键盘钢琴谱 QPO PQSSSSTS TUVVVVVVUTST TS S SQPO QRQPPQPP QPPPOOOO POO TUVVVVVVUTSSSQPO VVVVWWW
Counting stars Lately I've been, I've been losing sleep Dreaming 'bout the things that we could be But baby I've been, I've been prayin' hard Said no more counting dollars We'll be counting stars Yeah, we'll be counting stars I see this life Like a swinging vine Swing my heart across the line In my face is flashing signs Seek it out and ye shall find Old, but I'm not that old Young, but I'm not that bold And I don't think the world is sold I'm just doing what we're told I, feel something so right But doing the wrong thing I, feel something so wrong But doing the right thing
I could lie, could lie, could lie everything that kills me makes me feel alive Lately I've been, I've been losing sleep Dreaming 'bout the things that we could be Baby I've been, I've been prayin' hard Said no more counting dollars We'll be counting stars Lately I've been, I've been losing sleep Dreaming 'bout the things that we could be Baby I've been, I've been prayin' hard Said no more counting dollars We'll be, we'll be counting stars I feel the love And I feel it burn Down this river every turn Hope is a four letter word Make that money Watch it burn Old, but I'm not that old Young, but I'm not that bold
《童话》 LONOL LONOL LONO OOMML LONOL LQPPO LONOM MMOTS PPRRQQ QQNPOONO ONOR LSRQP PPRRQQ QQVUTUV VPOT TTSSS LSRQQRQ QRQ RQPO OQST TTSPPRQ OQST TTSPPRQRQPO PQMMOONO 《心语心愿》 TVUTSTQ TSTVUVUTUV VWXXXXW VUTUS TVUTST QSTXWVUV VUTTTTSSTQ SSTXWVUVV VUTTSUT 《会呼吸的痛》 STVTXXTW WWVUVWXWSV VUTUVRRRVVWVSSS SYXWXX STVTXXTW WWVUVWXWSVV VUTUVRRRVVWVSSS STUVVUVV 《欢乐颂》 J J K L L K J I H H I J J I I J J K L L K J I H H I J I H H I I J H I J K J H I J K J I H I E J J K L L K J I H H I J I H H 《小星星》 OOSSTTS RRQQPPO SSRRQQP SSRRQQP OOSSTTS RRQQPPO 《千千阙歌》 HHIJ LMONNNLJ IIIJK MOQPPNL HHIJ LMONNNLJ IIIJK MOQPPNL
MLMLMNNMN PPPPNOPQ QQPPPOQ NLM LMOPQQPQ QPOP OMM LMOP QQPQ QSTSQQ QQPPOPOM QQRQPOP QQ Q P POP OMOO 《婚礼进行曲》 HKKK HLJK HKNNMLKJKL HKKK HLJK HKMOMKILMK <雪绒花> JLP OLK JJJKLML JLP OLK JLLMNO O P LLNMLJLO MOPON L JLP OLK JLLMNO ONMLII JKLL NMLII JKLL 北京欢迎你 QSQPQPQ QPMOQP POMOPQSP QTSLPO POMOPQSP QTSSQ PQPOSTQ MQPPO QSVST STSQQSS QSTVWVSQPSQQ 《青花瓷》Flash键盘钢琴谱 LLJ IJF IJLJ I LLJ IJE IJLI H HIJLMLJ LJJI I HIH IHI IJLJ J LLJ IJF IJLJI LLJ IJE IJLIH HIJ LMLJ LJJII EJIIH
Lately, I’ve been, I’ve been losing sleep 最近我总是辗转反侧难以入眠 Dreaming about the things that we could be 对我们曾有过的愿景浮想联翩 But baby, I’ve been, I’ve been praying hard, 但亲爱的我早已在内心深处祈祷着 Said, no more counting dollars 祈祷自己不再迷失于金钱的追逐中 we’ll be countin g stars 我们可以细数满天繁星 Yeah, we’ll be counting stars 细数满天繁星品味生活乐趣 I see this life like a swinging vine 生活就像一株跃动的藤蔓 Swing my heart across the line 长驱直入激活我的内心 In my face is flashing signs 在我脸上划过迹象 Seek it out and you shall find 遍寻之后你能发现 Old, but I'm not that old 我虽上年纪但不致老态龙钟 Young, but I'm not that bold 虽还年轻却未必鲁莽失礼 I don't think the world is sold 坚信这个世界美好如初 I'm just doing what we're told 我只是循规蹈矩地生活着 I feel something so right Doing the wrong thing 惯于离经叛道中体味心安理得 And I feel something so wrong Doing the right thing 亦于按部就班中痛感乏善可陈 I could lie, could Iie, could lie 我欺骗过伪装过失真过 Everything that kills me makes me feel alive 但置我于死地者必将赐我以后生 Lately, I’ve been, I’ve been losing sleep 最近我总是辗转反侧难以入眠
英语儿歌《Counting Bananas》歌词 英语儿歌《Counting Bananas》歌词英语儿歌《Counting Bananas》歌词 One banana, two bananas,one, two, three. (Count to three on your fingers.) Three bananas for me! (Point to yourself.) Four bananas, five bananas,four, five, six. (Count to six on your fingers.) Six bananas for me! (Point to yourself.) Seven, eight, nine, ten! (Count to ten on your fingers.) Ten bananas for me! (Point to yourself.) Eleven, twelve, thirteen. (Pretend to pick bananas from a banana tree.) Thirteen bananas for me! (Point to yourself.) Fourteen, fifteen, sixteen. (Pretend to pick bananas from a banana tree.) Sixteen bananas for me. (Point to yourself.) Seventeen, eighteen, nineteen, twenty! (Pretend to pick bananas from a banana tree.) Twenty bananas for me. (Point to yourself.) Bananas to the left. (Put your hands together up high over
《痴心绝对》 个人收集整理勿做商业用途 《童话》 个人收集整理勿做商业用途 个人收集整理勿做商业用途 《下一站天后》 个人收集整理勿做商业用途 《安静》 个人收集整理勿做商业用途 《天空之城》 个人收集整理勿做商业用途 ... 个人收集整理勿做商业用途 《好好恋爱》 个人收集整理勿做商业用途 个人收集整理勿做商业用途 《梦中地婚礼》 个人收集整理勿做商业用途 个人收集整理勿做商业用途 《美丽地神话》 个人收集整理勿做商业用途 个人收集整理勿做商业用途 《卡农》括号表示两个键一起按，表示停顿 () () () 个人收集整理勿做商业用途 ()() () () () () () 个人收集整理勿做商业用途 ()() () () ()() () () 个人收集整理勿做商业用途 ()() () () ()()()()()()()()()()()()()()()()() 个人收集整理勿做商业用途() () ()() () () ()个人收集整理勿做商业用途 《遇见》
, , 《梦中地婚礼》键盘钢琴谱《想唱就唱》键盘钢琴谱 《梦里》键盘钢琴谱
马克西姆澄镜之水》 ()()()()()()()( : * }. ()()()()()()()()() ()()()()()`()()()()键盘钢琴论坛,键盘钢琴下载,键盘钢琴谱,键盘弹钢琴,键盘钢琴曲谱键盘钢琴谱" ()(;)()()()()()(;)()()()() ()()()()()()()()()键盘钢琴,键盘钢琴网,用键盘弹钢琴,键盘钢琴软件^ { ()()()()()()()()()键盘钢琴论坛,键盘钢琴下载,键盘钢琴谱,键盘弹钢琴,键盘钢琴曲谱键盘钢琴谱; , ; ()()`()()()()()(;)()()()- { , ) () ()()()()()()()()()()()() ()()()()()()()()()()()() ()();()(5C)();()()();()()();() $ ?. ) ; [()] 论坛钢琴,键盘钢琴谱,电脑弹钢琴,键盘弹钢琴谱,键盘钢琴下载,键盘钢琴谱,键盘钢琴曲谱,键盘弹钢琴,用键盘弹钢琴,键盘钢琴软件,键盘钢琴乐谱,流行歌曲键盘钢琴谱键盘钢琴谱,键盘钢琴乐谱键盘钢琴; " ()()()()()()()()()()键盘钢琴论坛钢琴,键盘钢琴谱,电脑弹钢琴,键盘弹钢琴谱,键盘钢琴下载,键盘钢琴谱,键盘钢琴曲谱,键盘弹钢琴,用键盘弹钢琴,键盘钢琴软件,键盘钢琴乐谱,流行歌曲键盘钢琴谱键盘钢琴谱,键盘钢琴乐谱键盘钢琴: ' \* ` * ()()()()()()()()()()键盘钢琴论坛,键盘钢琴下载,键盘钢琴谱,键盘弹钢琴,键盘钢琴曲谱键盘钢琴谱) " , ?' ()()()()()()()()()() ()()()()()`()()()()()() ()(;)()()()()()()()()() ()()()()()()()()()() ()()()()()()()()()()() ()()()()()()()()()()() ()()()()`()()()()()()键盘钢琴键盘钢琴研究学院$ : * ()()()()()() ()()()()()()()()() ()()()()()()()()()()()" ' " \ " $ } ()()()()()() `()()()()() ( , ?) ) ()()(;)()()()()() (]个人收集整理勿做商业用途
Counting Stars数星星 Performed by OneRepublic Lately, I've been, I've been losing sleep 最近阿我总是失眠 Dreaming about the things that we could be 织著我们的梦想 But baby, I've been, I've been playing hard, 但是宝贝阿我总是一直很努力 Sitting, no more counting dollars 坐著不再只数著钱 We'll be counting stars, yeah we'll be counting stars 我们来数星星来数星星吧 I see this life like a swinging vine 我看人生就像摇摆的藤蔓 Swing my heart across the line 动汤我的内心跨过这道线 And my face is flashing signs 我的脸就像那闪烁符号 Seek it out and you shall find 搜寻而你找到了 Oh, but I'm not that old 但我没那麼老 Young, but I'm not that bold 年轻但我没那麼英勇无谓 I don't think the world is sold 我不认为这是个被出卖的世界 I'm just doing what we're told 我只是做上头告诉我们该做的事 I feel something so right (我)感受到某些事是如此地正确 Doing the wrong thing 做著坏事 I feel something so wrong (我)感觉某些事错得一踏涂地 Doing the right thing 做对的事 I could lie, could lie, could lie
勿忘初心 Counting Stars Lately, I’ve been, I’ve been losing sleep 最近我总是辗转反侧难以入眠 Dreaming about the things that we could be 对我们曾有过的愿景浮想联翩 But baby, I’ve been, I’ve been praying hard, 但亲爱的我早已在内心深处祈祷着 Sitting, no more counting dollars 祈祷自己不再迷失于金钱的追逐中 we’ll be counting stars 我们可以细数满天繁星 Yeah, we’ll be counting stars 细数满天繁星品味生活乐趣 I see this life like a swinging vine 生活就像一株跃动的藤蔓 Swing my heart across the line 长驱直入激活我的内心 And my face is flashing signs 在我脸上划过迹象 Seek it out and you shall find 遍寻之后你能发现 Old, but I'm not that old 我虽上年纪但不致老态龙钟 Young, but I'm not that bold 虽还年轻却未必鲁莽失礼 I don't think the world is sold 坚信这个世界美好如初 I'm just doing what we're told 我只是循规蹈矩地生活着 I feel something so right when doing the wrong thing 惯于离经叛道中体味心安理得 And I feel something so wrong when doing the right thing 亦于按部就班中痛感乏善可陈 I could lie, coudn't I, could lie 我欺骗过伪装过失真过 Everything that kills me makes me feel alive 但置我于死地者必将赐我以后生 I feel the love and I feel it burn 我感觉到爱火在胸中熊起 Down this river, every turn 于河流的每个迂回处翻腾
《童话》Flash键盘钢琴谱 LONOL LONOL LONO OOMMLLONOL LQPPO LONOM MMOTS PPRRQQ QQNPOONO ONOR LSRQPPPRRQQ QQVUTUV VPOT TTSSSLSRQQRQ QRQ RQPOOQST TTSPPRQ OQST TTSPPRQRQPO PQMMOONO 《星语心愿》Flash键盘钢琴谱 TVUTSTQ TSTVUVUTUV VWXXXXW VUTUS TVUTST QSTXWVUV VUTTTTSSTQ SSTXWVUVV VUTTSUT 《会呼吸的痛》Flash键盘钢琴谱 STVTXXTW WWVUVWXWSV VUTUVRRRVVWVSSS SYXWXX STVTXXTW WWVUVWXWSVV VUTUVRRRVVWVSSS STUVVUVV 《欢乐颂》Flash键盘钢琴谱 J J K L L K J I H H I J J I IJ J K L L K J I H H I J I H H I I J H I J K J H I J K J I H I E J J K L L K J I H H I J I H H
《小星星》Flash键盘钢琴谱 OOSSTTS RRQQPPO SSRRQQP SSRRQQPOOSSTTS RRQQPPO 《千千阙歌》Flash键盘钢琴谱 HHIJ LMONNNLJ IIIJK MOQPPNLHHIJ LMONNNLJ IIIJK MOQPPNLMLMLMNNMN PPPPNOPQQQPPPOQ NLMLMOPQQPQ QPOP OMM LMOP QQPQ QSTSQQQQPPOPOM QQRQPOP QQ Q PPOP OMOO 《婚礼进行曲》Flash键盘钢琴谱 HKKK HLJK HKNNMLKJKL HKKK HLJK HKMOMKILMKNMLII JKLL NMLII JKLL HKKK HLJK HKMOMKILMKILMKK 《青花瓷》Flash键盘钢琴谱 LLJ IJF IJLJ I LLJ IJE IJLI H HIJLMLJ LJJI I HIH IHI IJLJ J LLJ IJF IJLJI LLJ IJE IJLIH HIJ LMLJ LJJII EJIIH
《童话》 LONOL LONOL LONO OOMML LONOL LQPPO LONOM MMOTS PPRRQQ QQNPOONO ONOR LSRQP PPRRQQ QQVUTUV VPOT TTSSS LSRQQRQ QRQ RQPO OQST TTSPPRQ OQST TTSPPRQRQPO PQMMOONO
《心语心愿》 TVUTSTQ TSTVUVUTUV VWXXXXW VUTUS TVUTST QSTXWVUV VUTTTTSSTQ SSTXWVUVV VUTTSUT 《会呼吸的痛》 STVTXXTW WWVUVWXWSV VUTUVRRRVVWVSSS SYXWXX STVTXXTW WWVUVWXWSVV VUTUVRRRVVWVSSS STUVVUVV
《欢乐颂》 J J K L L K J I H H I J J I I J J K L L K J I H H I J I H H I I J H I J K J H I J K J I H I E J J K L L K J I H H I J I H H 《小星星》OOSSTTS RRQQPPO SSRRQQP SSRRQQP OOSSTTS RRQQPPO
《千千阙歌》 HHIJ LMONNNLJ IIIJK MOQPPNL HHIJ LMONNNLJ IIIJK MOQPPNL MLMLMNNMN PPPPNOPQ QQPPPOQ NLM LMOPQQPQ QPOP OMM LMOP QQPQ QSTSQQ QQPPOPOM QQRQPOP QQ Q P POP OMOO
Counting Stars-OneRepublic 这首来自美国著名流行摇滚乐队OneRepublic的新单Counting Stars，收录在第三张专辑《Native》中。这首歌的歌词通过对一种内心挣扎无奈的描述，描绘了现在社会人们追名逐利，一切向利益看齐，迷失在金钱的迷雾中的社会现象。干脆利落的鼓点节奏仿佛试图敲响人们沉睡的心，歌曲旋律激昂，述说的何止是一种态度。 这首单曲发行不久后便迅速进入美国、英国、澳大利亚等多个国家的单曲排行榜前列，并且摘得了英国单曲榜的冠军。 歌词 Lately I been, I been losing sleep 最近我总是辗转反侧难以入眠 Dreaming ’bout the things that we could be 对我们曾有过的愿景，浮想联翩 But baby I been, I been prayin' hard 但亲爱的,我早已在内心深处祈祷着 Said no more counting dollars 祈祷自己不再迷失于金钱的追逐中 We'll be counting stars 我们可以细数满天繁星 Yeah, we'll be counting stars 细数满天繁星，品味生活乐趣 I see this life 生活就像 Like a swinging vine 一株跃动的藤蔓 Swing my heart across the line 长驱直入，激活我的内心 In my faces flashing suns 在我脸上，留下痕迹 Seek it out and ye shall find 遍寻之后，你能发现 Old, but I'm not that old 我虽上年纪，但不老态龙钟
26个字母 26个钢琴键请关闭汉字输入法点一下上面的FLASH才可正常使用可以用a-z来弹也可以用鼠标键盘钢琴谱键盘弹钢琴键盘钢琴下载键盘钢琴软件电脑键盘钢琴键盘钢琴曲谱钢琴键盘图 flash键盘钢琴用键盘弹钢琴 flash键盘钢琴谱 《童话》Flash键盘钢琴谱 LONOL LONOL LONO OOMMLLONOL LQPPO LONOM MMOTS PPRRQQ QQNPOON O ONOR LSRQPPPRRQQ QQVUTUV VPOT TTSSSLSRQQRQ QRQ RQPOOQST TTS PPRQ OQST TTSPPRQRQPO PQMMOONO 《星语心愿》Flash键盘钢琴谱 TVUTSTQ TSTVUVUTUV VWXXXXW VUTUS TVUTST QSTXWVUV VUTTTTSSTQ SSTXWVUVV VUTTSUT 《会呼吸的痛》Flash键盘钢琴谱 STVTXXTW WWVUVWXWSV VUTUVRRRVVWVSSS SYXWXX STVTXXTW WWVU VWXWSVV VUTUVRRRVVWVSSS STUVVUVV 《欢乐颂》Flash键盘钢琴谱 J J K L L K J I H H I J J I IJ J K L L K J I H H I J I H H I I J H I J K J H I J K J I H I E J J K L L K J I H H I J I H H 《小星星》Flash键盘钢琴谱 OOSSTTS RRQQPPO SSRRQQP SSRRQQPOOSSTTS RRQQPPO 《千千阙歌》Flash键盘钢琴谱 HHIJ LMONNNLJ IIIJK MOQPPNLHHIJ LMONNNLJ IIIJK MOQPPNLMLMLMNNMN PP PPNOPQQQPPPOQ NLMLMOPQQPQ QPOP OMM LMOP QQPQ QSTSQQQQPPOPO M QQRQPOP QQ Q PPOP OMOO 《婚礼进行曲》Flash键盘钢琴谱 HKKK HLJK HKNNMLKJKL HKKK HLJK HKMOMKILMKNMLII JKLL NMLII JKLL HKKK HLJK HKMOMKILMKILMKK 《青花瓷》Flash键盘钢琴谱 LLJ IJF IJLJ I LLJ IJE IJLI H HIJLMLJ LJJI I HIH IHI IJLJ J LLJ IJF IJLJI LLJ IJE IJLIH HIJ LMLJ LJJII EJIIH 《夜曲》Flash键盘钢琴谱
Video Article Counting Human Neural Stem Cells Steven Marchenko,Lisa Flanagan Department of Pathology,University of California,Irvine URL:https://www.doczj.com/doc/9d14197127.html,/index/Details.stp?ID=262 DOI:10.3791/262 Citation:Marchenko S.,Flanagan L.(2007).Counting Human Neural Stem Cells.JoVE.7.https://www.doczj.com/doc/9d14197127.html,/index/Details.stp?ID=262,doi:10.3791/262 Abstract Knowledge of the exact number of viable cells in a given volume of a cell suspension is required for many routine tissue culture manipulations,such as plating cells for immunocytochemistry or for cell transfections.This protocol describes a straightforward and fast method for differentiating between live and dead cells and quantifying the cell concentration and total cell number using a hemacytometer.This procedure first requires detaching cells from a growth surface and resuspending them in media.Next,the cells are diluted in a solution of Trypan blue (ideally to a concentration that will give 20-50cells per quadrant)and placed in the hemacytometer.Finally,averaging the counts of viable cells in several randomly selected quadrants,dividing the average by the volume of one 1mm 2quadrant (0.1μl)and multiplying by the dilution factor gives the number of cells per l.Multiplying this cell concentration by the total volume in μl gives the total cell number.This protocol describes counting human neural stem/precursor cells (hNSPCs),but can also be used for many other cell types. Protocol Passaging Human Neural Stem Cells Note :Please refer to the Passaging Human Neural Stem Cells article on how to detach and resuspend the cells. https://www.doczj.com/doc/9d14197127.html,/index/Details.stp?ID=263 Preparing the Cell Suspension for Counting Counting Cells in the Hemacytometer 1.Place 25μl of 0.4%Trypan Blue solution and 20μl of cell media into an eppendorf tube. 2.Resuspend the cells to be counted by gently tapping the tube containing the cells in a known volume of media to create a homogenous suspension.Place 5μl of cells into the eppendorf tube from the previous step.This step dilutes the cell concentration by a factor of 10. 3.Place the cover glass on the hemacytometer,and load about 11-12μl of the cell suspension. 1.Observe the entire grid of the hemacytometer in a phase contrast microscope.Focus on one quadrant of the grid (such as the one in red).