princ-ch23-presentation

肾孤立性纤维瘤1例李唐;王绍武;边杰;黄杰;孙卫兵;王齐敏【期刊名称】《中国临床医学影像杂志》【年(卷),期】2018(029)004【总页数】2页(P301-302)【关键词】肾肿瘤;纤维瘤;体层摄影术,X线计算机【作者】李唐;王绍武;边杰;黄杰;孙卫兵;王齐敏【作者单位】大连医科大学附属第二医院,辽宁大连116023;大连医科大学附属第二医院,辽宁大连116023;大连医科大学附属第二医院,辽宁大连116023;大连医科大学附属第二医院,辽宁大连116023;大连医科大学附属第二医院,辽宁大连116023;大连医科大学附属第二医院,辽宁大连116023【正文语种】中文【中图分类】R737.11;R730.262;R814.42病例男，26岁，外院体检超声发现左肾肿块1周后就诊本院。

临床查体均呈阴性。

本院超声检查：左肾中部一低回声光团，大小约34 mm×26 m m，向外凸，后方回声衰减，考虑肾癌。

CT平扫和增强扫描：左肾中部肾实质内见等及稍高密度实性肿块，向肾轮廓外及肾盂突出，最大层面约27mm×39mm，病变内未见坏死及囊变，未见明确脂肪密度，平扫CT值约27～39 HU（图1），增强扫描皮质期肿块强化程度明显低于周围相对正常的肾皮质，其内可见斑片状轻度强化，CT值约45～55 HU（图2）；髓质期肿块持续强化，呈斑片状明显强化灶，CT 值约60～75 HU（图3）。

影像诊断：左肾少血供肿块，考虑恶性肿瘤。

行左肾部分切除术，术中见肿瘤位于左肾中上极，偏腹侧，外生性，距肿瘤边界0.5 cm 处完整切除肿瘤。

大体观：肿瘤包膜完整，切面呈鱼肉样。

镜下观：肿瘤由梭形细胞构成，大部分细胞异型性不明显，少许细胞伴轻-中度异型（图4），细胞密集与疏松区交替分布丰富；肿物与肾组织界限尚清，其内可见较多血管，局部黏液样变。

免疫组化：肿瘤细胞 BCL-2 （+），CD34 （+），SMA 局部（+），EMA （±），HMB45（-），S-100（-），CD117（-），DOG-1（-），Desmin（-），Calponin（-），CD68（-），AE1/AE3（-），Ki-67 指数约 1%～3%。

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E v a l u a t i o nC e r t i f i c a t e Issuing Aut horit y NM i Certin B.V.22 December 2016C. Oost ermanHead C ert ificat ion BoardNM i Certin B.V. Hugo de Groot plein 1 3314 EG Dordrecht The Net herlandsT +31 78 6332332 cert in@nmi.nlwww.nmi.nl This document is iss ued under t he provis ion t hatno liability is accept ed and that the applicants hall indemnify third-part y liabilit y.R eproduction of t he completedocument only is permitt ed.Number TC10822 revision 0Project number: 16200475Page 1 of 1Issued by NM i C ert in B.V.In accordance w it h Aspect s of t he Volunt aryOIM L R117-Producer Endress + Hauser Flow t ec AGKägenst rasse 7C H-4153 ReinachS w it zerlandM easuring inst rument A flow transm itt er (calculat ing/indicat ing device for Endress + HauserC oriolis meters), int ended t o be used as a part of a measuring inst rument.Brand : Endress + Hauser Flow t ec AGDesignat ion : Promass 300S oft w are versions : see paragraph 1.2 of t he descript ionAccuracy class : 0,3Environment classes : M3 / E2Temperat ure range ambient: -40 °C +55 °CFurt her propert ies and t est result s are described in t he annexes:Descript ion TC10822 revision 0;Document at ion folder TC10822-1.R emarks An overview of t he performed t est s is given in Annex 2 appert aining t ot his Evaluat ion Cert ificat e.Number TC10822 revision 0Project number 16200475Page 1 of 51General inform ation on the flow transm itterAll propert ies of t his flow t ransmit t er, w het her ment ioned or not, shall not be in conflictw it h t he Legislat ion.This Evaluat ion Cert ificat e is t he posit ive result of t he applied volunt ary, modular approach, for a component of a measuring inst rument, as described in WELM EC guide 8.8.The complet e measuring inst rument must be covered by an EC-t ype examinat ion C ert ificat e or an EU-t ype examinat ion C ert ificat e.This Evaluat ion Cert ificat e is valid for t he Endress + Hauser flow t ransmit t er, as described in paragraph 1.1 of t he descript ion and may only be used in combinat ion w it h Coriolismeasurement sensors manufact ured by Endress + Hauser Flow t ec AG:- That are covered by an Evaluat ion Cert ificate;- Direct ly ment ioned in t he EU-t ype examinat ion cert ificat e of t he measuring syst em.1.1Essential PartsDescript ion Docum ent at ionRem arksnum berPow er Supply100 240 V AC / 50/60 Hz 10822/0-02; 10822/0-0324 V DC 10822/0-04; 10822/0-05100 240 V AC / 50/60 Hz;10822/0-06; 10822/0-0724 V DCA m plif ierFor Exi Hart10822/0-08; 10822/0-09 Zone 1 st andardFor Exi Hart10822/0-10; 10822/0-11 Zone 1 IIC 200 °CFor M odbus and Hart10822/0-12; 10822/0-13 Zone 2For Exd M odbus and Hart10822/0-14; 10822/0-15 Zone 1 st andardFor Exd M odbus and Hart10822/0-16; 10822/0-17 Zone 1 IIC 200 °CCPU M odemFor Exi hart devices 10822/0-18; 10822/0-19 I/O 1 = Exi current out put + HartFor Non-Ex Hart devices 10822/0-20; 10822/0-21 I/O 1 = C urrent out put + HartFor M odbus devices 10822/0-22; 10822/0-23 I/O 1 = M odbusI/O M odulesM ult i I/O module Non-Ex 10822/0-24; 10822/0-25 Frequency, pulse, st at us in/out,current in/outM ult i I/O module Ex 10822/0-26; 10822/0-27Double pulse 10822/0-28; 10822/0-29 Double pulse channel, phaseshift edDisplayDisplay module 10822/0-30; 10822/0-31 Int egrat ed in flow t ransmit t er orremot e (ext ernal) moduleNumber TC10822 revision 0Project number 16200475Page 2 of 5Descript ion Docum ent at ionRem arksnum berTerm inal boardsTerminal board 10822/0-32; 10822/0-33 For Ex devicesTerminal board 10822/0-34; 10822/0-35 For Non-Ex devicesTerminal board 10822/0-36 Used at remot e display moduleBack planeFor Ex devices 10822/0-37; 10822/0-38For non Ex devices 10822/0-39; 10822/0-40- Input s (sensor side)From t he connect ed C oriolis sensor t he Promass 300 receives:Tw o pick-of f signals; analogue mV signals from w hich t he mass flow and densit y aredeterminedOne t w o-w ire Pt-1000 t emperat ure t ransmit t er for t he measurement of t he t ubet emperat ureOne t w o-w ire Pt-1000 t emperat ure t ransmit t er for t he measurement of t he carriert ube t emperat ure.Please not e t hat t hese t emperat ures shall not be used for Cust ody Transfer relat edpurposes, but only for t he correct ion given above.- Out put s (sensor side)The Promass 300vibrat ing mot ion. The frequency of t he vibrat ion is aut omat ically adjust ed t o t heresonant f requency of t he measurement t ubes. This out put is referred t o as: drive current out put- Input/Out put (elect ronic calculat or/indicat ing device)The Promass 300 is equipped w it h t he follow ing C ust ody Transfer out put s: Double, 90° or 180° phase shif t ed pulse-out put for t he t ransmission of volume- ormass-informat ion. Phase shif t is select able4 20 mA out put for t he t ransmission of densit y. This out put can also be used forinput/out put via Hart prot ocol.Ot her informat ion can also be t ransmit t ed via t his out put (for example flow rat e), but t his informat ion is not W&M approved.4 20 mA input for t he t ransmission of product t emperat ure. The (ext ernal)t emperat ure is used for aut omat ic correct ion of t he t emperat ure influence on t heconnect ed measurement sensor and overrides bot h int ernal t w o-w ire Pt-1000t emperat ure sensors.4 20 mA input for t he t ransmission of product pressure. The pressure is used foraut omat ic correct ion of t he pressure influence on t he connect ed measurement sensor.S t at us input or st at us out put (depending on version)M odbus R S485 serial prot ocol. Dat a t ransmission is saf e guarded w it h C R C checksumover t he dat a package.Number TC10822 revision 0Project number 16200475Page 3 of 51.2Essential Characteristics-S oft w are t ype P;R isk C lass C;Ext ensions T and I-5 apply.- S oft w are-versionsVersion num bers Checksum01.00.02 0xE87F (M odbus)0x321F (Hart)The soft w are version and checksum can be verified on screen:S oft w are version, menus Diagnost ics Device Info Firmw are VersionC hecksum, menus Operat ion C ust ody Transfer ChecksumNot e t he s oft w are version includes t he soft w are of all modules ment ioned inparagraph 1.1.- C ust ody Transf er paramet ersThe C ust ody Transfer paramet ers and t he mandat ory set t ings are ment ioned in Annex 1.For securing t he Weight s and M easures paramet ers:Go t o menu S et up Advanced set up C ust.t rans. act.Login w it h aut horized user login and passw ord and complet e all ot her set t ings (dat eand t ime)S et dipsw it ch number 2 t o ON. Only t he Weight s and M easures paramet ers aresecured against changing.Aft er exit ing t he menu syst em, t he lock symbol () is show n on t he display and t hebot t om row show s t he Weight s and M easures count er.Alt ernat ive met hod is t o set dipsw itch number 1 to ON; t his secures all paramet ersagainst changing. The lock symbol () is show n on t he display.S ecurit y disabled S ecurit y enabled (paramet ers prot ect ed) - The flow t ransmit t er may only be used in combinat ion w it h an approved flow comput er[1] as t he display may not be used as a primary indicat ion.[1]The flow comput er must have an Evaluat ion or Part s Cert ificat e issued by a recognised Not ifiedBody under Annex M I-005 (M ID 2004/22/EC) or Annex VII (M ID 2014/32/EU).Number TC10822 revision 0 Project number 16200475 Page 4 of 51.3 Essential Shapes- Inscript ions of the Promass 300AtTC10822 is placed on t he housing(s) of the Promass 300; Ident ificat ion of t he measurementsensor; Serial number;In case of a remot e version: t he serial number of t he measurement sensor is inscribed on t he Promass 300 and/or t he serial number of t he Promass 300 is inscribed on t he measurement sensor. - S t at us of t he deviceIn case an elect ronic calculat or/indicat ing device is connect ed t o t he Promass 300, theis sent t o t he connect ed device in one of t he follow ing ways:-channels, t hus enabling t he flow comput er t o det ect a pulse failure and consequent ly generat e an alarm. Ifnecessary, an ext ra elect rical board is added t o prevent t his act ion short -circuit ing t hePromass 300.The current out put is set t o failure current (minimum or maximum value is possible), t hus enabling t he flow comput er t o det ect the failure.The Promass' pulse out put is set t o maximum pulse rat e for one channel and t o no pulses for t he ot her pulse channel. This w ill t rigger t he pulse securit y checking of t he connect ed elect ronic calculat or/ indicating device.The M odbus.In all cases t he st at us of t he device must be configured so, t hat t he alarm conditions- S ealing.S ee chapt er 2.- C ablingAll cables shall be shielded and t he shielding shall be connect ed on bot h sides.1.4 Conditional Part s- Key-boardThe keyboard consist s of a t hree keys of w hich t heir funct ion depends on t he menu and select ed it em. The keyboard is part of the LC D display. If t he display is not present , t he keyboard is also not present. - HousingInside t he met al housing t he Promass, t he essent ial and t he condit ional part s are mount ed. Access can be obt ained t hrough t w o sealable lids.S ee document at ion number 10822/0-01 for t he assembly of t he part s inside the housing. compact , explosion proof , direct ly mount ed on t he measurement sensor.Opt ionally t he display is mount ed in a separat e housing. The communicat ion w it h t he display is via M odbus.1.5 Conditional Shapes- Blinding of Promass displayIf an elect ronic calculat or/indicat ing device (flow comput er) is connect ed t o t he Promass 300, t he display (w hen present ) of t he Promass 300 may be blinded. The display is st ill pres ent and funct ioning, it is not possible t o read t he display.Number TC10822 revision 0Project number 16200475Page 5 of 52SealsAft er set t ing t he securit y sw it ch in t he secured posit ion, all lids are sealed against opening.3Conditions for Approval- Verify t he paramet ers set t ings ment ioned in Annex 1 for correct set t ing and w here applicable if t he set t ing is in accordance w it h t he calibrat ion dat a.- Ot her part ies may use t his Evaluat ion Cert ificat e only w it h t he w rit t en permission of Endress + Hauser Flow t ec AG, Kägenst rasse 7, CH-4153 R einach, S w it zerland.4Test ReportsAn overview of t he performed t est s is given in Annex 2 appert aining t o t his Evaluat ionC ert ificat e.Number TC10822 revision 0Project number 16200475Page 1 of 2M andat ory paramet er set t ings:General set t ings for all paramet ers (w here applicable):M ass unit: t, kg or gM ass flow unit: M et rical (for example kg/min)Volume unit: m3; dm3; cm3; L or mlVolume flow unit: M et rical (for example m3/h)Densit y unit: M et rical (for example kg/m3)Temperat ure unit: °C or KPressure unit: M et rical unitS imulat ion modes: S hall be disabledM enu Sub M enu Sub subm enuParam eter Value- DisplayLanguage Local language (preferred); EnglishOperat ionLocking S t at us C T act.; CT act.-def.par. S et up M ediumselect ionLiquidC urr.out put0/4 mA value S ee not e (1)20 mA valuePFS out put Assign pulse M ass flow or Volume flowValue perpulseS ee not e (2)Pulse w idt h S ee not e (3)Low flow cut off AssignvariableM ass flow or volume flow On value S ee not e (4)Off value S ee not e (5)Part ial pipe det AssignvariableDensit y Low value S ee not e (6) High valueAdvanced set up Tot aliser n Operat ionmodeS ee not e (7)Failure mode Act ual valueExpert S yst em Diagn.handlingAlarm delay 0Process param. Flow damping In accordance w it h t hecalibrat ion dat aDensit ydampingTemp.DampingNumber TC10822 revision 0Project number 16200475Page 2 of 2M enu Sub M enu Sub subm enuParam eter ValueExpert S ensor S ensor adjust. Inst all direct. C orrect inst allat ion direct ionshall be select ed.Variable adjust M ass flowoffsetS ee not e (8)M ass flowfact orS ee not e (9)Volume flowoffsetS ee not e (8)Volume flowfact orS ee not e (9) Densit y offset S ee not e (10) Densit y fact or S ee not e (10)C alibrat ion C al.Fact or According calibrat ion dat aNominaldiamet erC orrect sizeAccording calibrat ion dat aNot es t o t he mandat ory paramet er sett ings:(1)The sett ings in t he flow comput er shall mat ch t he set t ings in t he flow t ransmit t er.(2)The sett ings shall be such t hat t he maximum pulse frequency of 5000 Hz is not exceeded.(3)The value shall be such t hat t he pulses can st ill be processed by t he conn ect ed flow comput er.(4)Value at w hich t he low flow cut of f is enabled (pulse out put st opped)(5)Value at w hich t he low flow cut of f is disabled (pulsed st art ed). Is given as a percent age inrespect of t he On value.act ual value = On value x (1 + Off value/100).If t he act ual flow rat e is higher t han 20% of t he minimum flow rat e of t he (complet e)measuring syst em, pulses must be generat ed by t he flow t ransmit t er.(6)Values shall be explained during conformit y assessment(7)At leas t one of t he t ot alisers must be in accordance w it h t he calibrat ion dat a.(8)If t he mass or volume flow offset is not zero t he value shall be explained during t he conformit yasses sment for t he impact on t he meas urement performance. These paramet ers are notint ended for a correct ion of t he zero flow rate.(9)If t he mass/volume flow f act or is not 1 t he act ual value shall be explained during t heconformit y assessment for t he impact on t he measurement perf ormance.(10)If t he densit y off set and densit y fact or are not 0 resp. 1, t he act ual value s hall be explainedduring t he conformit y assessment.Number TC10822 revision 0Project number 16200475Page 1 of 1Performed t est s on t he flow transmit t er:TEST Part TYPE TEST REPORT TEST HOUSE REM A RKSC limat e , vibrat ion and EM C t est s according OIM L R117-2 Flowt ransmit t erPromass 300 NM i-16200475-01 NM i C ert in B.V.S oft w are Evaluat ion Flowt ransmit t erPromass 300 NM i-16200475-02 NM i C ert in B.V.。

大肠杆菌DNA复制相关蛋白PriC的初步研究孔敏敏;李艳华;董宇辉;王文雅;袁其朋【期刊名称】《中医学报》【年(卷),期】2011(26)6【摘要】目的:对大肠杆菌DNA复制相关蛋白PriC进行研究。

方法:构建重组表达载体pET21a-PriC及pET21a-PriC1-101,并进一步诱导表达纯化。

利用Pulldown手段将pET21a-PriC-notag与pET28at-plus-PriB分别转化到大肠杆菌BL21(DE3)中,诱导表达及共纯化后检测PriC与PriB的相互作用情况。

结果:PriC经Ni柱亲和层析纯化获得的蛋白稳定性较差,易沉淀。

根据二级结构和蛋白稳定性预测及限制性蛋白酶水解结果,构建了PriC的截短突变蛋白PriC1-101,经Ni亲和层析和superdex75纯化,获得了稳定性较好的蛋白,经Western blot鉴定为目的蛋白。

PriC1-101的圆二色谱分析发现其具有较多的α-helix和无规卷曲,这为更好的了解其结构奠定了重要的基础。

通过Pulldown手段发现PriC与PriB 存在相互作用,并且PriC的稳定性有所提高。

结论:PriC蛋白的C-端氨基酸序列是PriC蛋白与PriB蛋白相互作用的主要区域,并对PriC蛋白的稳定性有较大影响。

【总页数】4页(P689-692)【关键词】大肠杆菌;DNA;蛋白PriC【作者】孔敏敏;李艳华;董宇辉;王文雅;袁其朋【作者单位】北京化工大学生命科学技术学院;中国科学院高能物理研究所多学科中心【正文语种】中文【中图分类】R285.5【相关文献】1.重组肿瘤相关抗原蛋白与肿瘤治疗性DNA疫苗静电耦合成为复合纳米颗粒的初步研究 [J], 王浩;阎瑾琦;张飒;贾锐;张亮;刘宁;王宇;宋晓国;于继云2.乙肝病毒大蛋白检测及其与HBV-DNA复制的相关性 [J], 韩富秋;董海荣;嘎勒登玛3.大肠杆菌TorS/TorR二组分体应答蛋白TorR对DNA复制起始的影响 [J], 姚远;乔佳鑫;李静;李慧;莫日根4.拟南芥肌动蛋白相关蛋白基因AtARP5和AtARP6共同参与DNA复制 [J], 吴迪;安增选;樊天谊;朱炎因版权原因，仅展示原文概要，查看原文内容请购买。