H om e ?P roducts ?Library P reparation for N ext Generation Sequencing ?Library P reparation for N ext Generation Sequencing
Product Listing Product Overview
Library Preparation for Next Generation Sequencing
NEBNext? reagents are a series of highly pure reagents that facilitate sample
preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Av ailable in sets, master mix sets and modules, these robust reagents undergo stringent quality controls and functional v alidation, ensuring maximum yield, conv enience and v alue.
Each set of reagents is functionally v alidated together through construction of a library from an accepted DNA or RNA reference sample that is then sequenced on the
appropriate platform. Whenev er a new lot of any of the reagents is introduced into a set, the set is re-v alidated by library construction and sequencing.
NEBNext reagents are av ailable for sample preparation for Illumina?, 454?, SOLiD?and Ion Torrent? sequencing platforms in multiple formats, including reagents sets, master mix sets and modules.
NEBNext? is a registered trademark of New England Biolabs, Inc.
Illumina? is a registered trademark of Illumina, Inc.
454? is a trademark of Roche.
Ion Torrent? and SOLiD? are trademarks owned by Life Technologies, Inc.
Featured Products
NEBNext? Ultra? II DNA Library Prep Kit for Illumina?
NEBNext? Ultra? II Ligation Module
NEBNext Ultra II Q5 Master Mix
Videos
1 of 1
The common problem of adaptor dimer formation during small RNA library construction can be av oided by using NEBNext protocols. Learn about this technique, and how it improv es both performance and sensitiv ity in library construction.
A Breakthrough Method of RNA Sam ple Preparation
RNA Modules
Are adaptors or primers included in the NEBNext? products?
Are NEBNext? products v alidated in Next Generation Sequencing workflows?
Protocol for a PCR reaction using NEBNext? Ultra? II Q5? Master Mix (M0544)
Protocol for use with NEBNext? Ultra? II End Repair/dA-Tailing Module (E7546)
Protocol for use with NEBNext? Ultra? II Ligation Module (E7595)
Other Tools & Resources
Feature Articles
Addressing Challenges in Microbiome DNA Analysis
Publications related to Library Preparation for Next Generation Sequencing:
1. Saiful, I., Kj?llquist, U., Moliner, A. et al (2011). Characterization of the single-cell transcriptional
landscape by highly multiplex RNA-seq Genome Res. . 21, 1160-167.
2. Neiman, M., Lundin, S., Sav olainen, P., Ahmadian, A. (2011). Decoding a Substantial Set of Samples in
Parallel by Massiv e Sequencing PLoS ONE . 6, e17785. DOI: 10.1371/journal.pone.0017785
3. Yuan, B., Wang, J., Cao, H. et al (2011). High-throughput analysis of the mutagenic and cytotoxic
properties of DNA lesions by next-generation sequencing Nuc. Acids Res. . 39, DOI: 10.1093/nar/gkr159 4. Steele, P.R and Pires, J.C. (2010). Biodiv ersity assessment: State-of-the-art techniques in
phylogenomics and species identification Am. J. Bot. . 98, 415-25.
5. Feng, S., Cokusb, S.J., Zhang, X. et al (2010). Conserv ation and div ergence of methylation patterning in
plants and animals Proc. Natl. Acad. Sci. USA . 19, 8689-8694.
6. Johnston, J.J., Teer, J.K., Cherukuri, P.F. et al (2010). Massiv ely parallel sequencing of exons on the X
chromosome identifies RBM10 as the gene that causes a syndromic form of cleft palate Am. J. Hum.
Genet. . 86, 743-748.
7. Bonnefond, A., Durand, E., Sand, O. et al (2010). Molecular diagnosis of neonatal diabetes mellitus using
next-generation sequencing of the whole exome PLoS One . 10, e13630.
8. Schmidt, D., Wilson, M.D. Spyrou, C., Brown, G.D., Hadfield, J. and Odom, D.T. (2009). ChIP-seq: Using
high-throughput sequencing to discov er protein-DNA interactions Methods . 48, 240-48.
9. Smith, Z.D., Gu, H., Bock, C., Gnirke, A. and Meissner, A. (2009). High-throughput bisulfite sequencing
in mammalian genomes Methods . 48, 226-32.
10. Maricic, T. and P??bo, S. (2009). Optimization of 454 sequencing library preparation from small amounts
of DNA permits sequence determination of both DNA strands Biotechniques . 46, 51-2; 54-7.
11. Freeman, J.D., Warren, R.L., Webb, J.R., Nelson, B.H. and Holt, R.A. (2009). Profiling the T-cell receptor
beta-chain repertoire by massiv ely parallel sequencing Genome Res. . Jun. 13,
12. Green, R.E., Krause, J., Ptak, S.E., Briggs, A.W., Ronan, M.T., Simons, J.F., Du, L., Egholm, M.,
Rothberg, J.M., Paunov ic, M. and P??bo, S. (2006). Analysis of one million base pairs of Neanderthal DNA Nature . 444, 330-6.
13. Johnsson N. and Johnsson K. (2003). A fusion of disciplines: chemical approaches to exploit fusion
proteins for functional genomics Chembiochem . 4, 803-810.
Advantages
Convenient Formats – All of the required enzymes, buffers and nucleotides are included in our sets and modules; many are av ailable in master mix format. Sets for Small RNA and for Ion Torrent include adaptors and primers. Modules offer the ability to customize sample preparation.
Functional Validation – Each reagent set or module is functionally v alidated by preparation of a genomic DNA library that is then sequenced using the appropriate sequencing platform (Illumina, Roche/454, SOLiD or Ion Torrent) and by preparation of an expression library or single-stranded DNA or by preparation of a library from an accepted RNA reference sample.
Stringent Quality Controls – Additional QCs ensure maximum quality and purity.
Value Pricing
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