禽流感與人畜共通疾病研討會
第四屆沙門氏菌暨其他細菌性人畜共通疾病研討會
Fourth National Symposium on Salmonellelosis
and other bacterial zoonosis
議程及論文集摘要
Final Program and Abstract
時 間：民國98年9月12日 (星期六)
主辦單位：國立嘉義大學
生命科學院 微生物與免疫學系/生物醫藥科學研究所
農 學 院 獸醫學系 動物科學系
農業委員會 動植物 HYPERLINK "https://www.doczj.com/doc/323127479.html,/_ylt=A3eg8p8AU_hJJ5MAY0Fr1gt.;_ylu=X3oDMTE1MmNkZDh1BHNlYwNzcgRwb3MDMQRjb2xvA3R3MQR2dGlkA1RXQzA1N18yMTk-/SIG=11dsps2cb/EXP=1241097344/**http%3a/https://www.doczj.com/doc/323127479.html,.tw/" \t "_blank" 動植物防疫檢疫局
地 點：國立嘉義大學蘭潭校區圖資大樓演講廳
（嘉義市學府路300號）
禽流感與人畜共通疾病研討會
第四屆沙門氏菌暨其他細菌性人畜共通疾病研討會
時間：民國98年9月12日 (星期六)
主辦單位：國立嘉義大學 生命科學院 微生物與免疫學系/生物醫藥科學研究所
農 學 院 獸醫學系 動物科學系
農業委員會 動植物 HYPERLINK "https://www.doczj.com/doc/323127479.html,/_ylt=A3eg8p8AU_hJJ5MAY0Fr1gt.;_ylu=X3oDMTE1MmNkZDh1BHNlYwNzcgRwb3MDMQRjb2xvA3R3MQR2dGlkA1RXQzA1N18yMTk-/SIG=11dsps2cb/EXP=1241097344/**http%3a/https://www.doczj.com/doc/323127479.html,.tw/" \t "_blank" 動植物防疫檢疫局
地點：國立嘉義大學蘭潭校區圖資大樓演講廳（嘉義市學府路300號）
議程表
時 間 內 容 主持人 演講人 08:30-09:00 報到 (Registration) 09:00-9:20 開幕典禮(Opening remarks) 校長、院長 09:20-109:510 專題演講
家禽流行性感冒與人類的關係Convergent evolution of Salmonella typhoid agents 張伯俊 陳秋麟
國立嘉義大學劉樹林
北京大學 09:50-10:20 專題演講： HYPERLINK "javascript:openProject('1201746071222',%20'D',%20'T',%20'97',%20'C','')" 以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒 陳秋麟朱紀實 張伯俊
國立中興大學 10:1020-10:3040 Break 禽流感與其他細菌人畜共通傳染病 10:3040-1011:5010 專題演講:
禽流感之基因重組桿狀病毒疫苗之開發談家禽流行性感冒與人類的關係 蘇耀期劉宏仁
劉宏仁
國立屏東科技大學陳秋麟
國立嘉義大學 11:10-11:30 乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於Methicillin與Vancomycin之抗藥性情形專題演講: 禽流感之基因重組桿狀病毒疫苗之開發 劉宏仁張伯俊 蘇耀期
國立嘉義大學劉宏仁
國立屏東科技大學 11:30-11:50 Necrotizing fasciitis and mastitis associated methicillin resitance Staphyloccus aureus乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於Methicillin與Vancomycin之抗藥性情形 張簡志炫
嘉義基督教醫院蘇耀期
國立嘉義
大學 11:50-13:00 Lunch (Poster session) 流行病學 與治療 13:00-13:15 犬隻沙氏桿菌分子流行病學之調查 余章游 廖明輝
國立屏東科技大學 13:15:13:30 High mobility group 1 (HMGB1) protein as the therapeutic target for severe sepsis and septic shock 金立德
國立嘉義大學 13:30-13:45 嘉義地區沙門氏菌在水禽幼雛及蛋膜感染 金立德 余章游
國立嘉義大學 13:45-14:00 Salmonella Virchow質體增加在THP-1細胞中存活能力及Salmonella Virchow SGI-2之演化 李永茹
國立嘉義大學 14:00-14:20 合照 14:20-15:00 Break (Poster session) 致病機制與遺傳 (Pathogenesis and Genetics) 15:00-15:15 Immunity of Chicken to Salmonella enterica serovar Enteritidis infection 鄧景浩 朱紀實
國立嘉義大學 15:15-15:30 A constitutively mannose-sensitive agglutinating Salmonella enterica serovar Typhimurium LB5010 strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes 葉光勝
臺北醫學大學 15:30-15:45 Why Salmonella enterica serotype Choleraesuis is more invasive than serovar Typhimurium? 葉光勝朱紀實 邱政洵
長庚林口醫院 15:45-16:00 Identification and Characterization characterization of Escherichia coli Ggenes eEpidemiologically aAssociated with Urinary urinary Tract tract iInfections 鄧景浩
國立成功大學 16:00-16:30 Panel Discussion
陳秋麟、余章游、蘇耀期、陳國隆、朱紀實、葉光勝 Lecture section
Convergent evolution of Salmonella typhoid agents -------- P
劉樹林
北京大學、哈爾濱醫科大學、CUniversity of Calgary
家禽流行性感冒與人類的關係
陳秋麟
國立嘉義大學獸醫學系
HYPERLINK "javascript:openProject('1201746071222',%20'D',%20'T',%20'97',%20'C','')" 以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒
張伯俊1、謝快樂2、沈瑞鴻2
1國立中興大學微生物暨公共衛生學研究所, 2國立中興大學獸醫學系
禽流感之基因重組桿狀病毒疫苗之開發
劉宏仁
國立屏東科技大學 生物科技研究所 獸醫學系
乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於Methicillin與Vancomycin之抗藥性情形
蘇耀期、李研懷
國立嘉義大學獸醫學系
Methicillin-resistant Staphylococcus aureus associated necrotizing fasciitis and mastitis
Chih-Hsuan Changchien1, 2, Wan-Lin Chen2, and Chishih Chu1*
Department of Microbiology and Immunology, National Chiayi University1; Department of Plastic and Reconstructive Surgery, Chiayi Christian Hospital2
犬隻沙氏桿菌分子流行病學之調查
1劉紋紋 2陳玄峰 1盧明志 1廖明輝
1國立屏東科技大學獸醫學系 2國立屏東科技大學生物科技研究所
8. High mobility group 1 (HMGB1) protein as the therapeutic target for severe sepsis and septic shock
Li-T
e Chin
國立嘉義大學微生物與免醫學系
9. 嘉義地區沙門氏菌在水禽幼雛及蛋膜感染
Chang-You Yu,*§ Jiang-Liang Lin,* Chishih. Chu,* Yao-Chi Su,§ Shu-Wun Chen, * Pei-Chun Tu,
§Department of Veterinary Medicine, *Department of Microbiology and Immunology, National Chiayi University
The change of Salmonella genomic island and R plasmid-mediating survival in THP-1 cells for Salmonella enterica serovar Virchow
Yung-Lu Lee1, Chien-Shun Chiou2, Chishih Chu1,
1Graduate Institute and Department of Microbiology and Immunology, National Chiayi University, 2The Central Branch Office, Center for Disease Control
11. Immunity of Chicken to Salmonella enterica serovar Enteritidis infection
Chishih Chu1, Kuo-Lung Chen2, Chang-You Yu3
Department of Microbiology and Immunology1, Department of Animal Sciences2, Department of Veterinary Medicines3, National Chiayi University
12. A constitutively mannose-sensitive agglutinating Salmonella enterica serovar Typhimurium LB5010 strain, carrying a transposon in the fimbrial usher gene stbC, exhibits multidrug resistance and flagellated phenotypes
1吳冠勳, 2王克銓, 3許元勳, 3葉光勝
1台北市立萬芳醫院小兒科, 2臺北醫學大學醫學科學研究所,
3臺北醫學大學醫學系微免學科
13. Why Salmonella enterica serotype Choleraesuis is more invasive than serovar Typhimurium?
K.-Y. Huang1, I.-H. Wang1, L.-C. Wang1, C.-H. Chiu1,2
1Graduate Institute of Basic Medical Science, Chang Gung University College of Medicine and 2Department of Pediatrics, Chang Gung Children’s Hospital, Chang Gung University College of Medicine
14. Identification and Characterization of Escherichia coli Genes Epidemiologically Associated with Urinary Tract Infections
Bin-Hsu Mao, Wen-Jun Huang, and Ching-Hao Teng
Institute of Molecular Medicine, National Cheng Kung University School of Medicine, Tainan city, Taiwan
Poster section
1. Differences in characteristics between Streptococcus agalactiae fish and human isolates
Pei-Yu Huang1, Yu-Hsin Chang1, Feng-Ting Chang1, Jiun-Nun Hou2, Chishih Chu2, Chun-Che Chen1
Graduate Institute of Aquatic Biosciences, National Chiayi University1
Department of Microbiology and Immunology, National Chiayi University2
2. Plasmid, antibiotic susceptibility and pulsed field gel electrophoresis (PFGE) analysis of Salmonella Pullrum
Yi-Chun Chen1, Shu-Wun Chen1, Shu-Lin Liu2 and Chishih Chu1
1 Department of Microbiology and Immunology, National Chiayi University, Taiwan, ROC and 2Department of Microbiology, Peking University Health Science Center, Beijing, PROC
3. Differences in antimicrobial susceptibility, resistance genes. plasmid types between blood and respiratory Escherichia coli isolates
Yi-Lin Tsai1, Nuo-Shian Wang1, Yi-Hsuan Chen1, Shu-Wun Chen1, Jain-Shing Liu2, and Chishih Chu1
Department of Microbiology and Immunology, National Chiayi University1
Tainan Hospital, Department of
Health, Executive Yuan, R.O.C.2
4. Antimicrobial resistance to erythromycin and fluorioquinolone and their resistance genes in Streptococcus agalactiae
Yi-Rou Wang1, Jiun-Nun Hou1, Ting-Yu Lin 1, Mei-Hui Wang2, Ying-Shiang Wang2, Chishih Chu1
Department of Microbiology and Immunology, National Chiayi University1; Department of Pediatrics, Chiayi Chang-Gung Memorial Hospital2
5. Genetic variations and antimicrobial susceptibility of clinical Salmonella enterica serotype Stanley isolates in 2004 - 2007 from Taiwan
Li-Ting Sun1, Dvi-Wei Fan1, Shu-Wun Chen1, Chien-Shun Chiou2, Chishih Chu1
Department of Microbiology and Immunology, national Chiayi University,1
The Central Branch Office, Center for Disease Control, Taichung, Taiwan2
6. 乳牛披衣菌感染之疫情探討
陳賢文、余章游
國立嘉義大學獸醫學系
7. Pathogenicity and antibiotic Resistance in Streptococcus iniae
Feng-Ting Chang, Bin-Hong Hsu, Che-Chun Chen
Department of Aquatic Biosciences, National Chiayi University
8. 山羊流產披衣菌
李志展
國立嘉義大學獸醫學系
9. 嘉南地區種禽場沙門氏菌疫情調查
申國榮、郭慧蓮、辜啟瑞、吳晟瑋、蔡宗展、陳秋麟、余章游
國立嘉義大學獸醫學系
10. The protective effect of β-glucan from different sources on Salmonella Enteritidis invasion in chicks
May-Ling Ho*, Ling-Ping Huang, Tsai-Tzu Chen, Kuo-Lung Chen
Department of Animal Science, National Chiayi University
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沙門氏菌的分子進化Molecular Evolution of Salmonella leading to distinct pathogens
人畜共患病感染因子宿主適應性的遺傳學基礎Genetic basis of host adaptation in pathogenic bacteria that infect both humans and animals
腫瘤疫苗、HIV疫苗研究
1998.10-Present
Adjunct Research Professor, Department of Microbiology and Infectious Diseases, University of Calgary, Faculty of Medicine, Calgary, AB, Canada.
Research: Genomic divergence and evolution of Salmonella.
1996.12-1998.9
Research associate, Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, Calgary, AB, Canada.
Research: Bacterial genomic evolution.
1992.06-1996.11
Research associate, Department of Biological Sciences, University of Calgary, Calgary, AB, Canada. Worked on bacterial genomics.
Research: Construction and comparison of physical maps of Salmonella species.
1990.03-1992.05
Postdoctoral fellow, Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.
Research: Physical mapping
of S. typhimurium LT2, S. enteritidis, S. paratyphi B.
會員關係及學術兼職
American Society for Microbiology會員
中國藥典委員會第8屆委員
北京微生物學會理事
雜誌編委Journal Editorship
Member of Editorial Board of Journal of Microbiology and Immunology
Member of Editorial Board of International Journal of Genetics
國內外科研基金審閱External Grant Review
中國國家科技部973重大傳染病專案
美國自然科學基金會National Science Foundation (NSF) of United States
中國國家自然科學基金委National Natural Science Foundation of China (NSFC)
稿件審閱 (Manuscript review for the following journals)
Journal of Bacteriology (ASM, USA)
Microbiology (France)
Canadian Journal of Microbiology (Canada)
Genome Biology (UK)
Emerging Infectious Diseases (USA)
中華微生物學和免疫學雜誌Chinese Journal of Microbiology and Immunology (China)
Nucleic Acid Research (USA)
Journal of Clinical Microbiology (ASM, USA)
Functional and Integrative Genomics (Germany)
Hereditas (Sweden)
International Journal of Immunogenetics (UK)
Journal of Microbiology and Immunology (中華微免英文版，China)
中華醫學雜誌Chinese Journal of Medicine (China)
中國生物化學和分子生物學報
北京大學學報（醫學版）
Journal of Life Sciences (China)
BMC Microbiology (UK)、
BMC Genomics (UK)、
BMC Bioinformatics (UK)
Infection and Immunity (ASM, USA)
Cell Biology International (UK)
Veterinary Microbiology (Elsevier)
Applied and Environmental Microbiology (ASM, USA)
Cancer Letters (Elsevier)
Genomics (Elsevier)
生理科學進展
國際遺傳學雜誌
Genome Research
Journal of Biomedicine and Biotechnology
專利
A biological method for production of enterodiol. Application number: 200810104215.2
A novel method for construction of tumour vaccines based on attenuated Salmonella. Application number: 200710179692.0
發表論著
I. 原始論文 (2007-2009最近五年)
Qing-Hua Zou, et al., Molecular characterization of a novel prophage of Salmonella paratyphi C. Under review by J Bacteriol.
Fang Chen, et al., mutL regulates genetic stability through conversion between two allele states: a role in bacterial evolution at the population level. Under review by Genetics.
Wei-Qiao Liu, et al., Genome comparison of Salmonella typhimurium DT104 strains: multi-resistance not exclusively determined by SGI-1. Under review by FEMS Microbiology Letters.
Fang Chen, et al., 2009. A Genome Map of Salmonella enterica serovar Agona: Numerous Insertions and Deletions Reflecting the Evolutionary History of a Human Pathogen. FEMS Microbiology Letters. 293: 188-195.
Wei-Qiao Liu, et al., 2009. Salmonella paratyphi C: diverging from S. choleraesuis and converging with S. typhi. PLoS ONE. 4(2): e4510.doi:10.1371/journal.pone.0004510
Ye-Jun Wang, et al., 2008. Two oral HBx vaccines delivered by live attenuated Salmonella: both eliciting effective anti-tumor immunity.
Cancer Letters. 263: 67-76.
Zheng, X., S.-L. Liu. 2008. The relaxing ori-ter balance of Mycoplasma genomes. Science in China. 51 (2): 1-8.
Liu, W.-Q., et al., 2007. Diverse genome structures of Salmonella paratyphi C. BMC Genomics. 8: 290.
Gong, J., et al., 2007. Spontaneous conversion between mutL and 6bp?mutL in Salmonella typhimurium LT7: association with genome diversification and possible roles in bacterial adaptation. Genomics. 90: 542-549.
汪業軍，侯豔，黃鶴，劉桂榮，劉樹林。2007。沙門菌III型分泌蛋白融合HBx抗原介導的T細胞免疫效果觀察。中華微生物學和免疫學雜誌。27：719-724。
嚴岩，李彤，劉樹林。2007。兩種培養基對銅綠假單胞菌和大腸埃希菌生物被膜厚度的影響。中國實驗診斷學(Chin J Lab Diagn)。11（5）：12-17。
劉桂榮，劉葳嶠，祁丹妮，何曉燕，張鳳民，劉樹林。2006。雞沙門菌基因組物理圖。中華微生物學和免疫學雜誌。26：965-970。
部小霞，Aaron White，王玲，劉樹林。2006。沙門菌細聚合菌毛作為新型載體表達外源基因片段。中華微生物學和免疫學雜誌。26：974-978。
Liu, G.-R., et al., 2006. Genome plasticity and ori-ter rebalancing in Salmonella typhi. Molecular Biology and Evolution. 23 (2): 365-371.
Wu, K.-Y., et al., 2005. The genome of Salmonella gallinarum: distinct insertions/deletions and rare rearrangements. J. Bacteriol. 188: 4720-4727.
Kothapalli, S., et al., 2005. Diversity of genome structure in Salmonella enterica serovar Typhi populations. J. Bacteriol. 187: 2638-2650.
II. 綜述與合著
S.-L. Liu. 2007. Physical Mapping of Salmonella Genomes. In “Methods in Molecular Biology”, Heide Schatten and Abraham Eisenstark (Eds), The Humana Press.
Liu, S.-L., X. Zheng, L. Wang, H. Ling, and G.-R. Liu. 2004. Racing with HIV-1: challenges and hopes. Journal of Microbiology and Immunology. Vol. 2 (1): 1-5.
劉樹林，龔俊. 2004. 細菌進化中的基因橫向轉移。Horizontal gene transfer in bacteria during evolution. 中華微生物學和免疫學雜誌Chinese Journal of Microbiology and Immunology. Vol. 24 (6): 498-504.
龔俊，劉樹林2004 空腸彎麴菌與大腸彎麴菌基因分型研究進展Typing of Campylobacter jejuni and Campylobacter coli. 中華微生物學和免疫學雜誌 Chinese Journal of Microbiology and Immunology. Vol. 24 (5): 414-8.
衣美英，朱萬孚，劉樹林2003 基因組結構在細菌種系發育研究中的應用 Genome structure and bacterial phylogeny. 中華微生物學和免疫學雜誌 Chinese Journal of Microbiology and Immunology. Vol. 23 (12): 995-998.
Sanderson, K. E., M. McClelland and S-L. Liu. 1999. Chapter II.8, P. 217-233. "Stable" Genomes. Invited chapter in "Organization of the Prokaryotic Genome", Robert Leo Charlebois (ed), ASM Press.
Liu, S-L. and K. E. Sanderson. 1998. Bacterial genome analysis, Vol. 27, P. 371-381. In "Methods in Microbiology: Ba
cterial Pathogenesis", P. H. Williams, J. Ketley, and G. Salmond (eds), Academic Press, N.Y.
Sanderson, K. E., and S.-L. Liu. 1998. Chromosomal rearrangements in enteric bacteria. A review in Electrophoresis 19: 569-572.
Sanderson, K. E., S.-L. Liu, A. Hessel, and M. McClelland. 1998. Genome evolution in the Salmonellae, P. 230-239. Invited chapter in "Bacterial Genomes: Physical Structure and Analysis", F. de Bruijn, J. Lupski, and G. Weinstock (eds), Chapman and Hall.
Liu, S-L. and K. E. Sanderson. 1998. Salmonella typhi, P. 733-736. Invited chapter in "Bacterial Genomes: Physical Structure and Analysis", F. de Bruijn, J. Lupski, and G. Weinstock (eds), Chapman and Hall.
Liu, S-L. and K. E. Sanderson. 1998. Salmonella typhimurium, P. 737-740. Invited chapter in "Bacterial Genomes: Physical Structure and Analysis", F. de Bruijn, J. Lupski, and G. Weinstock (eds), Chapman and Hall.
Sanderson, K. E., A. Hessel, S.-L. Liu, and K.E. Rudd. 1996. The genetic map of Salmonella typhimurium, edition VIII pp.1903-1999. In Escherichia coli and Salmonella: Cellular and Molecular Biology, Edition 2, F.C. Neidhardt, R. Curtiss III, C.A. Gross, et al., (eds.). American Society for Microbiology, Washington, D.C.
Liu, S-L., K-K. Wong, M. McClelland, and K. E. Sanderson. 1993. Construction of a physical map of the genome of Salmonella typhimurium, p. 41-50. In F. Cabello, C. Hormaeche, P. Mastroeni, and L. Bonina (ed.), Biology of Salmonella. Plenum Press, New York and London.
Liu, S-L., and K. E. Sanderson. 1993. Physical map of Salmonella typhimurium LT2., p. 2.91-2.92. In S. J. O'Brien (ed.), Genetic maps, 6th ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
Liu, S-L. 1982. The non-A, non-B hep
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id, a potentially fatal systemic infection. It is not known whether the typhoid agents have evolved from a common ancestor (by divergent processes) or acquired similar pathogenic traits independently (by convergent processes). Comparison of different typhoid agents with non-typhoidal Salmonella lineages will provide excellent models for studies on how similar pathogens might have evolved. We sequenced a strain of S. paratyphi C, RKS4594, and compared it with previously sequenced Salmonella strains. RKS4594 contains a chromosome of 4,833,080 bp and a plasmid of 55,414 bp. We predicted 4,640 intact coding sequences (4,578 in the chromosome and 62 in the plasmid) and 152 pseudogenes (149 in the chromosome and 3 in the plasmid). RKS4594 shares as many as 4346 of the 4,640 genes with a strain of S. choleraesuis,
which is primarily a swine pathogen, but only 4008 genes with another human-adapted typhoid agent, S. typhi. Comparison of 3691 genes shared by all six sequenced Salmonella strains placed S. paratyphi C and S. choleraesuis together at one end, and S. typhi at the opposite end, of the phylogenetic tree, demonstrating separate ancestries of the human-adapted typhoid agents. S. paratyphi C seemed to have suffered enormous selection pressures during its adaptation to man as suggested by the differential nucleotide substitutions and different sets of pseudogenes, between S. paratyphi C and S. choleraesuis.
陳秋麟 教授
國立嘉義大學 農學院? 獸醫學系
現 職：教授兼主任秘書
學 歷：國立中興大學獸醫學博士(2004畢業)
國立中興大學獸醫學碩士(1985/09至1987/06)
台灣省立嘉義農業專科學校獸醫科(1968/09至1974/06)
簡要經歷：
國立嘉義技術學院畜牧科講師、副教授
台灣省政府農林廳畜牧科家畜衛生股長
台灣省立嘉義農業專科學校獸醫科助教
(A)期刊論文
???? 1. 陳秋麟、謝快樂。1987。台灣鵝的網狀內皮增殖病及其病原性研究。台灣省畜牧獸醫學會會報。49: 1-19。
???? 2. 陳秋麟。1990。馬立克病和傳染性華氏囊病疫苗接種對肉雞新城雞病免疫及生長性能之影響。台灣省畜牧獸醫學會會報。56: 1-14。
???? 3. 吳建平、陳國隆、洪炎明、陳秋麟。1992。白肉雞育雛開始三天之飲水中添加糖對死亡率及生長性能之影響。台灣省畜牧獸醫學會會報。60: 43-50。
???? 4. 吳建平、陳國隆、陳秋麟、洪炎明。1992。蔗糖添加方式對肉雞生長性能及屠體品質之影響。嘉義農專學報。31: 77-86。
???? 5. 陳國隆、連塗發、吳建平、陳秋麟、洪炎明。1993。產前(18週齡 )強制拔毛對來航蛋雞產蛋性狀之影響。嘉義農專學報。32: 57-63。
???? 6. 項延塏、林哲夫、蘇耀期、王建雄、陳運雄、陳秋麟。1993。台灣北部地區乳羊飼養環境與寄生蟲感染之關係。嘉義農專學報。34: 15-30。
???? 7. 吳建平、陳國隆、洪炎明、陳秋麟。1993。高溫及採血方法對荷蘭牛血漿中兒茶酚胺濃度與泌乳量之影響。嘉義農專學報。35: 59-66O
???? 8. 連塗發、陳世宜、陳秋麟、吳建平。1993。日糧中添加不同水準之比碇甲酸鉻對肉豬生長性能及血液性狀之影響。中國畜牧學會會誌。22(4): 349-357。
???? 9. Lian, T.F. et al., 1996. Effects of chromium picolinate and chromium chloride on growth performance and serum traits of growing-finishing swine. Proceedings, West. Sect., A.Socie. Anim. Sci. 47: 150-153.?
?? 10. 陳國隆、陳秋麟、連塗發、洪炎明。1997。飼糧中不同銅含量對蛋小雞組織殘留及血清生化值之影響。台灣省畜牧獸醫學會會報。67: 39-44。
?? 11. 陳國隆、吳建平、陳秋麟。1997。飼糧中不同銅含量對蛋小雞生產性狀與小腸組織結構之影響。嘉義農專會報。50: 31-39。
?? 12. 楊程堯、張伯俊、沈瑞鴻、陳秋麟、李龍湖、謝快樂。1997。台灣新近分離新城雞病病毒野外株之毒力與核酸序列分析。中華民國獸醫學會雜誌。23(1): 34-42。
?? 13. Chiou, et al., 1999. Effect of dietary copper on the morphology of gastro-intestinal tract in broiler chickens. Asian-Aus. J. Anim. Sci. 12(4): 548-553.
?? 14. 吳建平、陳國隆、陳秋麟、林炳宏。1999。飼糧中添加菸鹼酸鉻對0-8週齡火雞生長性能、屠體性狀及血液生化值之影響。國立嘉義技術學院學報。63: 1-9。
?? 15. 王璧娟、連塗發、陳秋麟、林高塚。2000。牛蒡對老鼠血糖、血脂質與尿酸的影響。台灣農業化學與食品化學。38(2): 181-183。
2.
?? 16. 陳國隆、陳秋麟、吳建平。2000。不同灌食次數及縮短灌食期間對土番鴨生產肥肝之影響。中華生質能源會誌。19(1-2): 1-9。
3.
?? 17. 龔順榮、張伯俊、陳秋麟、謝快樂。2000。台灣雞源與鴨源分離之敗血性巴斯德桿菌之分子特性。中華民國獸醫學會雜誌。26(3): 233-241。
4.
?? 18. Chen CL, Chang MS, Lee MS, Shien JH, Ou SJ, Shieh HK 2003, Nucleotide sequences of goose circovirus isolated in Taiwan Avian Pathology 32:165-171.
?? 519. 陳秋麟、張伯俊、謝快樂、李敏旭、歐繕嘉、陳建弘、沈瑞鴻。2004。應用聚 合
酶連鎖反應檢測水禽中之鵝環狀病毒。台灣獸醫誌。30(1):84-90。
?? 20 6. 陳秋麟、張伯俊、沈瑞鴻、龔順榮、李敏旭、陳建弘、謝快樂。2004。台灣鵝
環狀病毒之分子流行病學調查與病毒定量技術。台灣獸醫誌。30(2):156-162。
?? 721. 吳建平、陳秋麟、蔡秀敏、陳國隆。2004。性別對來航雞隻免疫反應之影響。嘉大農林學報。1：85-96。
?? 822. 吳建平、陳秋麟、李宗育、陳國隆。2004。菸鹼酸鉻對肥育期豬隻生長、血液及屠體性狀之影響。中國畜牧學會會誌。33(4)：271-277。
239. 陳秋麟、李敏旭、張淑卿、黃郁珳、羅登源。2006。台灣火雞禽痘感染。嘉大農林學報。3：99-107。
1024. Chen CL. et al., 2006. Development of a Polymerase Chain Reaction Procedure for Detection and Differentiation of Duck and Goose Circovirus. Avian Diseases 50: 92-95.
1125. 張淑卿、龔順榮、施良儒、*陳秋麟。2006。台灣火雞鼻氣管鳥桿菌（Ornithobacterium rhinotracheale）細菌分離及血清學調查。台灣獸醫學雜誌。32：180-185。
1226. Lee MS, et al., 2006. Genetic and Pathogenic Characterization of H6N1 Avian Influenza Viruses Isolated in Taiwan between 1972 and 2005. Avian Diseases 50: 561-571.
1327. Ching-Ho WANG, et al., 2007. Serological Prevalence of Salmonella Enterica Serovars Enteritidis and Pullorum
in Different Types of Chickens in Taiwan During 2005. Taiwan Vet J 33: 14-19.
1428. Dan-Yuan Lo, et al., 2007. Appendicular Telangiectatic Osteosarcoma. J Agric For (NCYU). 4: 1-9.
O12 談家禽流行性感冒與人類的關係
禽流感和人類的關係
陳秋麟 教授
國立嘉義大學獸醫學系
流行性感冒病毒屬於正黏液病毒科 (Orthomyxoviridae family)，根據其核蛋白及基質蛋白的抗原差異分為甲、乙或丙型（Type A,B,C）。禽流感病毒(AIV)屬於甲型（Type A），目前分為高病原性禽流感病毒（HPAI）和低病原性禽流感病毒（LPAI），依國際疫會規定只要是H5或H7不論HPAI或LPAI均需通報，然後依一定程序鑑定後採取既定防疫措施。禽流感病毒冇種表面抗原-血液凝集素 (haemagglutinin, H)及神經胺酸酶 (neuraminidase, N)，能引起特定亞型及免疫反應，目前有16種H亞型 (H1-16) 及 9種N亞型 (N1-9)。禽流感病毒對鳥類的內皮起源 (腸，肺) 之上皮組織的主要受體種類-α2,3連結的唾液酸接受體結合位置（receptor-binding site）有高親合性，因此主要影響禽鳥，對雞隻和火雞具有高致病力，但因病毒會不定時基因突變，衍生新品種，導致原來僅感染禽類的流感病毒，變得可以影響人類；此外，已研究證實人類氣管、支氣管和小支氣管內有一定數目的纖毛細胞同樣帶有較低密度像禽類之接受體，對於與受感染的活家禽、被其糞便嚴重污染的表面或物件有密切接觸的人，感染的風險極高。1997年於香港爆發H5N1，當時受感染的18人中有6人死亡，在流行病學上與活禽市場的高度接觸有關；2003年末再度發生H5N1亞型高病原性禽流感，接著由東北亞、東南亞並傳至蘇聯和歐洲，由於這些突變的流感病毒對人類是全新的病毒，因此曾和病禽或其病源接觸的人導致嚴重病症，自2003年起累計至2009年8月31日止全球共發生440病例，262人死亡。由於這高致病性禽流感H5N1病毒株有特異性能跳躍不同物種類障礙和在人類引起高致死率嚴重疾病，文獻指出A型流感病毒在不同物種間的傳播以及重組曾見於豬、人、鴨及火雞，引起1957及1968年世界大流行的人類流感病毒均含有類似禽類流感病毒的基因片斷，因此，全球仍關注其是否在人類提升另一全世界流行性感冒大流行的可能性，當人（高度風險群）同時感染禽流感與和流行性感冒兩種病毒，這兩種病毒在其體內可能經互換基因而衍生新變種病毒，如果這新病毒會發生直接從人對人 (代替只從鳥-禽-畜對人)傳染，預期將會遇見全世界流行性感冒大流行開始。今年以來雖然因為在全球多國發生H1N1新型流感的泛流行疫情而轉移焦點，但仍應提高警覺，隨時注意高致病性禽流感H5N1
的後續發展。
張伯俊 教授
?學歷：
國立陽明大學生物化學研究所博士
?經歷：
國立中興大學獸醫微生物學研究所長 (2006.09.01~)
國立中興大學獸醫微生物學研究所教授
美國Stanford大學遺傳研究所博士後研究
國立陽明大學微生物及免疫研究所博士後研究
發表期刊論文著作
M.-S. Lee, et al., Genetic and Pathogenic Characterization of H6N1 Avian Influenza Viruses Isolated in Taiwan between 1972 and 2005. Avian Diseases 2006;
C.-L. Chen, et al., Development of a Polymerase Chain Reaction Procedure for Detection and Differentiation of Duck and Goose Circovirus. Avian Diseases 2006; 50: 92-95
C.-Y. Wang, et al., Expression of Capsid Proteins and Nonstructural Proteins of Waterfowl Parvoviruses in Escherichia coli and Their Application to Serological Assay. Avian Pathology 2005; 34: 1-7.
H.-J. Tsai, et al., Genetic variation of viral protein 1 genes of field strains of waterfowl parvoviruses and their attenuated derivatives. Avian Diseases 2004; 48:512-521.
H. K. Shieh, et al., Complete Nucleotide Sequences of S1 and N Genes of Infectious Bronchitis Virus Isolated in Japan and Taiwan. Jouranl of Veterinary Medical Science 2004. 66: 555-558
J.-R. Wu, et al. 2003. Molecular characterization of plasmids with antimicrobial resistant genes in avian isolates of Pasteurella multocida. Avain Diseases. 47: 1384-1392 (SCI)
C.-L. Chen, et al., 2003. Nucleotide sequences of goose circovirus isolated in Taiwan. Avian Pathology. 32, 165-171.
J.-H. Shien, et al., 2002. Characterization of Newcastle disease virus isolated from Taiwan in 1998-2000. J. Chin. Vet. Sci. 28, 1-8.
P.-C. Chang, et al., Expression of Infectious Laryngotracheitis Virus (ILTV) Glycoproteins in Escherichia coli and Their Application in Enzyme-linked Immunosorbent Assay. Avian Diseases 2002,46, 570-580.
O23 HYPERLINK "javascript:openProject('1201746071222',%20'D',%20'T',%20'97',%20'C','')" 以Multiplex RT-PCR與PCR/RFLP技術快速檢測及區分H5、H6亞型禽流感病毒及高病原性新城病毒
張伯俊1、謝快樂2、沈瑞鴻2
1國立中興大學微生物暨公共衛生學研究所, 2國立中興大學獸醫學系
中文摘要
本研究建立以multiplex RT-PCR配合RFLP技術，以快速鑑定及區分禽流感病毒(AIV)與新城病毒(NDV)，並可快速區分AIV之H5或H6，以及N1或N2亞型，另外也可區分NDV之疫苗株與田間強毒株。在AIV與 NDV之區分方面，以AIV之NP基因引子對配合NDV之F基因引子對
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of avian influenza virus (AIV) and Newcastle disease virus (NDV). This method could also differentiate between H5, H6, N1 and N2 subtypes of AIV and differentiate vaccine strain from field strains of NDV. For identification of AIV and NDV, multiplex RT-PCR using primer pairs specific for the NP gene of AIV and for the F gene of NDV gave the best sensitivity and specificity. For differentiating between H5/H6 and N1/N2, multiplex RT-PCR using primer pairs H5-310/H6-730 and N1-365/H639 gave the best sensitivity and specificity. For RFLP analyses, the RT-PCR product amplified from the H5 gene was subjected to HaeIII and AluI digestion and the RFLP patterns obtained could differentiate H5 viruses (H5N2) of American lineage from those of Eurasian lineage (H5N1). In summary, the multiplex RT-PCR and RFLP method developed in this study could be used for rapid identification and differentiation of H5N1, H5N2, H6N1 virues and NDV in 5-6 hrs, and circumvent the need for sequence analyses.
劉宏仁 教授兼生技所所長
國立屏東科技大學 獸醫學系/ 生物科技研究所
分子病毒學暨生物技術研究室
學歷：
(1)美國奧本大學博士（1996年）:主修分子病毒學、生物技術及禽病學
(2)加拿大Manitoba大學醫學院內科及生理系博士後研究(1996-1997):
主持人經歷:
現職：國立屏東科技大學 生物科技研究所 教授兼所長 2006/08-迄今 國立屏東科技大學 獸醫學系 教授 2003/08-迄今 經歷: 南部科學園區
亞洲基因科技股份公司 董事會 獨立董事 2006/01-2009/06 財團法人生物技術開發中心
國立屏東科技大學
獸醫學系 專案顧問
副教授 2007/01-2008/12
2000/08- 2003/07 國立屏東科技大學 獸醫學系 助理教授 1997/08- 2000/07 加拿大Manitoba大學醫學院 內科及生理系 博士後研究員 1996/07- 1997/07 台灣省家畜衛生試驗所 豬瘟系 助理(公務人員高等考試及格分發) 1987/09-1991/08
研究成果目錄： (2007-2009)
1. Julius L. et al., Apoptosis induction by avian reovirus through P53 and mitochondrial pathways. BBRC 356: 529-535, 2007. (IF: 2.65) (SCI)
2 Hsin Y. et al., Apoptosis induction by avian reovirus related to tissue injury. Avian Pathology 36 :155-159, 2007. (IF: 1.7) (Vet. Sci. 18/134) (SCI)
3. Tang, D. G., et al., Display of avian influenza virus hemagglutinin on baculovirus envelope: effect of cytoplasmic domain on virus properties and potentials as vaccine. Molecular Therapy 15: 989-996, 2007. Corrigendium 15(9):1736-1736. (IF: 5.97) (SCI)
4. Hsiao-Ping Lee, et al., Variation of baculovirus-harbored transgene transcription among mesenchymal stem cell-derived progenitors leads to varied expression. Biotechnology & Bioenginering 97: 649-655, 2007. (IF: 2.94) (SCI)
5. Y. Y. Lien, et al., Cloning and nucleotide sequencing of the seco
nd internal transcribed spacer of ribosomal DNA for three species of Eimeria from chickens in Taiwan. The Veterinary Journal 173 : 186-191, 2007. (IF: 1.8) (Vet. Sci. 15/ 134) (SCI)
6.Yung Fu Wu, et al., Sequence and phylogenetic analysis of interleukin (IL)-1(-encoding genes of five avian species and structural and functional homology among these IL-1( proteins. Veterinary Immunology and Immunopathology 116:37-46, 2007. (IF: 1.91) (Vet. Sci. 12/134) (SCI)
7. Su, Y. P., et al., Avian reovirus core protein {micro}A expressed in Escherichia coli possesses both NTPase and RTPase activities. J. General Virology 88: 1797-1805, 2007. (IF: 3.05) (SCI)
8. Shen, P. C., et al., Genetic variation of the λA and λC protein encoding genes of avian reovirus. Research in Veterinary Science 83: 394-402, 2007. (IF: 1.38) (Vet. Sci. 34/134) (SCI)
9. Sue T. et al., Suppression of bovine ephemeral fever virus by RNA interference. Journal of Virological Methods 145: 84-87, 2007. (IF: 2.08) (SCI)
10. Chi Y. Wang , et al., The cleavage of the hemagglutinin protein of avian influenza virus in Pichia pastoris. J. Virological Methods 146:293-297, 2007. (IF: 2.08) (SCI).
11. Liu, H. J.* and Yao C. Hsieh. Control and prevention of avian influenza virus. Science Development 417: 62-69, 2007.
12. Julius L. C. et al., Avian reovirus p17 protein causes G2/M cell cycle arrest through inhibition of p34Cdc2. J. International Cooperation, 2: 12-25, 2007.
13. Wen T. et al., Supression of S-class genes of avian reovirus by RNA interference. Veterinary Microbiology 129: 252-261, 2008. (IF: 2.37) (Vet. Sci. 5/134) (SCI).
14. Chi-Young Wang, et al., Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of Newcastle disease virus. Veterinary Microbiology 130: 28-36. 2008. (IF: 2.37) (Vet. Sci. 5/134) (SCI).
15. Wu, Y. P., et al.,. Avian reovirus gene expression is required for the induction of chicken IL-1B mRNA expression in chicken peripheral blood mononuclear cells. J. General Virology 89:1059-1068, 2008. (IF: 3.05) (SCI)
16. Shu K. Li, et al., Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of avian reovirus. J. Virological Methods 49: 35-41, 2008. (IF: 2.08) (SCI).
17. Yu T. Chen, et al., Proteasome inhibition reduces avian reovirus replication and apoptosis induction in cultured cells. J.Virological Methods 151: 95-100, 2008. (IF: 2.08) (SCI).
18. Hung-Jen Liu . et al., Activation of small Gtpase Rho A and Rac 1 is required for avian reovirus p10-indiuced syncytiun formation. Molecule and Cells 26: 396-403, 2008. (IF: 1.92) (SCI)
19. Xin-Gang Xu, et al., Baculovirus surface display of Erns envelope glycoprotein of classical swine fever virus. J. Virological Methods 153:149-155, 2008 (IF:2.08) (SCI).
20. Ching-Ju Yeh, et al., TNFα mediates pseudorabies virus-induced apoptosis via theact
ivation of p38 MAPK and JNK/SAPK signaling. Virology 581:55-66, 2008. (IF 3.54) (SCI).
21. Review article: Wen T. Ji, Hung J. Liu*. PI3K-Akt signaling and viral infection. Recent Patent on Biotechnology 2 (3): 218-226, 2008. (SCI)
22. Xin-Gang Xu and Hung-Jen Liu*. Baculovirus surface display of E2 envelope glycoprotein of classical swine fever virus and immunogenicity of the displayed proteins in mouse model. Vaccine 26: 5455-5460, 2008. (IF: 3.3) (Vet. Sci. 1/134) (SCI).
23. Yueh H. et al., Baculovirus surface display of σC and σB proteins of avian reovirus and immunogenicity of the displayed proteins in a mouse model. Vaccine 26: 6361-6367 2008. (Vet. Sci. 1/134) (IF 3.3) (SCI).
24. Ping-Yuan Lin, et al., Modulation of p53 by mitogen-activated protein kinase pathways and protein kinase C delta during avian reovirus S1133-induced Apoptosis. Virology 385:323-324, 2009. (IF 3.54) (SCI)
25. Chi H. Lin, et al., Bovine ephemeral fever virus-induced apoptosis requires virus gene expression and activation of Fas and mitochondrial signaling pathway. Apoptosis 14: 864-877, 2009. (IF: 3.97) (SCI)
26. Wen T. Ji et al.,. Avian reovirus influences phosphorylation of several factors involved in
host protein translation including eukaryotic translation elongation factor 2 (eEF2) in Vero
cells. BBRC 384: 301-305, 2009. (IF: 2.65) (SCI)
27. Julius L. et al., Cell cycle regulation. Recent Patent on Biotech.3 (1): 1-9, 2009. (SCI)
28. Hung-Jen Liu , et al., Introduction of recombinant baculovirus of avian influenza. Veterinary Medical Association of Tainan County 14 : 96-117.
29. Xin-Gang Xu, et al., Baculovirus surface display of NS3 nonstructural protein of classical swine fever virus. J. Virological Methods 159:259-264, 2009. (IF: 2.08) (SCI).
30. Hung-Jen Liu. Introduction of immunoglobulin in yolk (IgY). CCPC Magazine 71: 28-33, 2009.
31. Yao-Ching Hsieh, et al., Diagnosis of avian tuberculosis in Swinhoe’s Pheasants using conventional and molecular-based techniques. Avian Diseases, 2009 (in press) (IF:1.54) (Vet. Sci. 30/134) (SCI).
32. Hsieh, C. Y., et al., Isolation and identification of a new fish pathogen, Francisella-like bacterium (FLB) causing systemic granulomas in tilapias. J. Fish Diseases, (in press)
33. Zi-Jiang Yang , et al., Development o
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neral Virology, ( Revised). (SCI)
36. Julius L. C. et al., Avian reovirus p17 protein causes G2/M cell cycle arrest. The Veterinary Journal, . (in review) (SCI).
37. Wen T. et al., Bovine ephemeral feve
r virus diffenertly regulates the activity of Akt and
mTOR complexes 1. Veterinary Journal (submitted) (SCI).
38. Guan M. et al., An amino acid residue substitution at the heptad repeat region in the stalk of the Newcastle disease virus HN glycoprotein impairs its hemagglutinin activity. Avian Pathology. (SCI).
39. Feng-Lang Lin and Hung-Jen Liu*. Antiproliferative activity of a natural compound from Morinda citrifolia and its effects on apoptosis in human hepatoma SKHep 1. BBRC, (in review) (SCI).
40. Wen T. Ji and Hung J. Liu*. 5-amino-4-imidazolecarboxamide ribose (AICAR) down-regulates multiplication of bovine ephemeral fever virus in Vero cells. Journal of Virological Methods, (in review) (SCI).
41. Chi-Young Wang, et al., Development of a reliable assay protocol for identification of diseases (RAPID)-bioactive amplification with probing (BAP) for detection of H5 avian influenza virus. J. Virological Methods, (in review) (SCI).
(二)專利
1. 胡育誠、楊定罡、劉宏仁 以假模式桿狀病毒促進對抗禽流感的免疫性」
2007. (美國專利 申請中)
2. 胡育誠、楊定罡、劉宏仁 以假模式桿狀病毒促進對抗禽流感的免疫性」
2007. (台灣專利 申請中)
3. 劉宏仁、李淑桂 偵測不同血清型及基因型家禽里奧病毒之磁珠核酸探針檢測方法. 2008. (台灣專利; 申請中)
4. 劉宏仁、林岳宏. 泛用型桿狀病毒表面呈現系統之開發及應用於次單位疫苗製備 2008. (台灣專利; 申請中)
O34 禽流感之基因重組桿狀病毒疫苗之開發
劉宏仁 教授兼所長
國立屏東科技大學 生物科技研究所, 獸醫學系
Baculovirus display系統已成為研發次單位疫苗之重要工具。被表現之蛋白質可表現於桿狀病毒之封套，形成所謂 ”重組桿狀病毒”。此重組桿狀病毒可作為次單位疫苗。家禽流行性感冒病毒(avian influenza virus ; AIV)為正黏液病毒科(Orthomyxoviridae)之A型流行性感冒病毒。高病原性家禽流行性感冒(highly pathogenic avian influenza, HPAI)對雞或火雞造成高度傳染或急速死亡。Hemagglutinin (HA)和Neuraminidase (NA)為家禽流行性感冒病毒套膜上的主要免疫原。傳統的作法利用桿狀病毒系統所表現的目標蛋白存在於昆蟲細胞細胞質中。我们成功將H5N2禽流感HA及NA蛋白以鑲嵌的方式表現在桿狀病毒外套膜，以製備基因重組桿狀病毒。我们研究亦發現若以原本屬於桿狀病毒包膜蛋白gp64之胞質區段(cytoplasmic domain，CTD) 來取代HA蛋白本身之CTD可更有效率地將此HA蛋白鑲嵌並表現在桿狀病毒的外套膜上。我們以Baculovirus display表現平台構築了兩種禽流感之重組桿狀病毒(Bac-HA64 & BacSC-NA)，經由免疫螢光/共軛焦顯微鏡的觀察，兩種病毒在感染昆蟲細胞後，皆可以有效地表現重組HA及NA蛋白於細胞膜上。而由免疫金電子顯微鏡
觀察，兩種重組蛋白也都可以展現在病毒的套膜上。為進一步探討研發之基因重組桿狀病毒是否適合開發成有效的次單位疫苗，因此進行雞隻疫苗效力及攻毒實驗免疫實驗，結果顯示雞隻經Bac-HA64重組桿狀病毒次單位疫苗免疫兩次後，HI抗体可達64-128倍以上並完全保護雞隻攻毒後免於致病。另HI抗体力價達16倍以上者之雞隻使用108.5 ELD50之H5N1病毒攻毒可得完全保護效果。這些結果顯示HA抗原適合開發成有效的次單位疫苗，用以防備禽流感。
蘇耀期 副教授
國立嘉義大學 農學院? 獸醫學系
學 歷：台灣大學獸醫學 博士
簡要經歷：
1977-1979年任職美國雅培藥廠動物部業物代表。
1981年公務人員高等考試暨專門職業技術人員高等考試獸醫類組及格。
1983-1986年任職南投縣政府家畜疾病防治所技士。
1986-1987年任職省農林廳畜牧科技士。
1985-1987年兼任國立嘉義農專獸醫科講師。
1987-1989年任職國立嘉義農專畜產科專任講師。
1989-1999年任國立嘉義農專及嘉義技術學院畜產系專副教授。
1991-1994年兼任國立嘉義農專進修部教務組組長。
1999年 國立嘉義大學農學院獸醫學系專任副教授迄今。
(A)期刊論文
1. Chiu, S.Y., et al., 2000. Studies of eyes of pursang cat(s): (II) Anisocornea, concomittent iris reaction, and unilateral iris response upon light illumination in relation to the distances of light source. A. Environ. Microbiol. (Vol. II) 8: N0.4 117-122.
2. Su, Y.C., Feng, C.Y. 2000. Endoparasites of Ardeidae birds in Taiwan. AEMppl. Environ. Microbiol. (Vol. III) 9:11-18.
3. Lin, P.H., Su, Y.C. 2000. The Effects of Native Chickens Inoculated Eimeria tenella and Dietary Diclazuril Supplementation on the Growth Performance and Immune Response. Jour. of the Biomass Energy Society of China.? 18(3-4 ): 151-159.
4. Su, Y.C., et al., 2000. Antibiotic sensitivity of pathogenic isolates: (V) Durgs having isolates with moderate sensitivity of gram nagative bacilli during 1995-1998. AEMppl. Environ. Microbiol. (Vol. II) 8:101-107.
5. Mar, P.H., Su, Y.C. Fei, A.C.Y. Bowman, D.D. 2000. A survey of endoparasitic zoonoses of stray dogs and cats in Taipei city. Appl. Environ. Microbiol. Vol. II 8(3):77-86.
6. Chiu, S.Y. et al., 2000. Legal infection disease : (IV) distribution of case numbers and chronological curves of Japanese B encephalitis of last decade in the republic of china. AEMppl. Environ. Microbiol. Vol. III 9( 1):3-9.
7. Chiu, S.Y. et al., 2000.Studies of AIDS and HIV-seropositive infections in the republic of china: (III) Comparative analysis of AIDS/HIV-1 seropositive case numbers of various administrative areas of this country. Microbiol. Vol. III 9(2):35-47.
8. Chiu, S.Y. et al., 2001. Interaction of Snail and Tropical Fishes In Aqua Environme
nt L1) Study of Movements of Snail(s), The Speed, Dirrections Under Room Temperature. Appl. Environ. Microbiol. 10: 9-15.
9. Shiu SY, et al., 2002. Studies of body temperatures(1) Body temperatures of varies animals icluding human beings. A. Environ. Microbiol. 10: 65-73.
10.. Chiu SY, et al., 2001. Interaction of Snail snail and Tropical tropical Fishes fishes In in Aqua Environment L1) Study of Movements of Snail(s), The Speed, Dirrections Under Room Temperature. AEMppl. Environ. Microbiol. 10: 9-15.
11. Su YC, et al., 2003. Differential diagnosis of five avian Eimeria species by polymerase chain reaction using primers derived from the internal transcribed spacer1 (ITS-1) sequence.. Vet. Parasitol. 117: 221-227. (SCI)
12. Shiu SY, et al., 2004. Antibiotics with moderate percentages of sensitive isolates during 1998-2001. A. Environ. Microbiol. 12: 57~64.
13. Su YC, Fei ACY. 2004. Endoparasites of the Crested Goshawk, Accipiter trivirgatus formosae, from Taiwan, Republic of China. Comparative Parasitology. 71(2), 178~183. (SCI)
14. 謝志宏等、朱紀實、蘇耀期、王誠明、余章游。2008。家禽種禽及孵化場之沙門氏桿菌疫情與抗藥性分析。嘉大農林學報5:70-86。
15. Chang-You Yu, et al., 2008. Comparison of the association of age with the infection of Salmonella and Salmonella enterica serovar Typhimurium in Pekin ducks and Roman geese. Poultry Science.
(SCI, impact factor: 1.747; 15.9% top, 7/44; in press)
16. Maw-Rong Chao, et al., 2007. Assessing the Prevalence of Salmonella enterica in Poultry Hatcheries by Using Hatched Membranes. Poultry Science.86:1651-1655. (SCI, impact factor: 1.747)
O54 乳羊分離之Staphylococcus aureus與Staphylococcus lentus對於
Methicillin與Vancomycin之抗藥性情形
蘇耀期、李研懷
國立嘉義大學獸醫學系
摘 要
葡萄球菌在人及動物為普遍造成食物中毒、傷口及醫源性的感染源，由於金黃色葡萄球菌具強烈致病性與對抗生素具有抗藥性，相關的研究也很多。金黃色葡萄球菌引起之牛羊乳房炎亦為一重要的問題，為瞭解乳羊場分離的葡萄球菌之抗藥性的分佈情形，首先將臨床分離菌株(2006~2008 分離之S. aureus113株及S. lentus 31株)進行16種抗生素敏感性試驗，將所獲得之結果進行分析後，發現普遍地存在著多重抗藥性，其中2006~2007分離之MRSA為0%(0/86)，VRSA為64.0%(55/86)，而2008年分離之MRSA則為44.4%(12/27)， VRSA為29.6%(8/27)；另外2008年分離之MRSL為100%(31/31)，VRSL為96.8%(30/31)；將此菌株再輔以最小抑菌濃度(MIC)試驗，將兩者結果進行分析與比較發現MRSA MIC50 為16， MIC90 為>256，VRSA MIC50 為 16~24，MIC90 為48，另MRSL MIC50和 MIC90 分別為>256，VRSL 的MIC50 和MIC90分別為32及64 均呈現抗藥性質。我們繼續探討乳羊與人源性MRSA的MecA與VacA,B,C,D基因檢測及脈衝電泳分析其同源關係
。
張簡志炫 醫師
嘉義基督教醫院整形外科
學歷
中山醫學大學醫學系
國立嘉義大學微免所
經歷
高雄長庚醫院整形外科主治醫師
中華民國整形外科專科醫師
中華民國美容外科醫學會會員
Publications
1. Chang-Chien CH. HYPERLINK "https://www.doczj.com/doc/323127479.html,/pubmed/17113524?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum" Bacteraemic necrotizing fasciitis with compartment syndrome caused by non-O1 Vibrio cholerae. J Plast Reconstr Aesthet Surg. 2006;59(12):1381-4.
2. Chang-CHien CH. et al., Bacteremic necrotizing facsciitis with compartment syndrome caused by non-O1 Vibro cholerae. JPRAS. 2006; 59:1381-1384.
3. Chang-Chien CH, Ding HT, Liu C, Yang CS. HYPERLINK "https://www.doczj.com/doc/323127479.html,/pubmed/16945371?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum" Vibrio infection associated with finning injury of the hand. Injury. 2007 May;38(5):614-8.
O5 Methicillin-resistant Staphylococcus aureus associated necrotizing fasciitis and mastitis
Chih-Hsuan Changchien1, 2, Wan-Lin Chen2, and Chishih Chu1
Department of Microbiology and Immunology, National Chiayi University1; Department of Plastic and Reconstructive Surgery, Chiayi Christian Hospi
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al, and microbiological features of MRSA with NF and mastitis, and (2) examine the choice of empiric antibiotic therapy.
Methods: Staphylococcal chromosomal cassette mec (SCCmec) types, pulse-field gel electrophoresis (PFGE) patterns, and virulence factors of 16 MRSA isolated from NF patients and 11 MRSA isolated from mastitis patients in 2008 were determined. Medical records were reviewed, including demographic characteristics, comorbid underlying conditions, and clinical features.
Results: Most MRSA NF infections were usually monomicrobial. Over 90% of MRSA isolates were resistant to erythromycin and clindamycin. Resistance to trimethoprim-sulfamethoxazole (SXT) significantly differed between HA-MRSA isolates and CA-MRSA isolates. Molecular analysis of 16 available MRSA isolates indicated that the SCCmec III and nontypeable type were only identified in HA-MRSA, whereas SCCmec IV and V were present in the 6 CA-MRSA isolates. All isolates possessed γ-hemolysin and only CA-MRSA isolates encoded Panton-Valentine leukocidin (PVL) genes. Virulence factors TSST-1, ETA, and ETB of MRSA were rarely detected among NF patients.
Of the
mastitis-associated MRSA isolates, 10/11 (90.9%) was HA-MRSA (i.e., a history of hospitalization, surgery in the past year). However, antimicrobial resistant patterns and PCR results indicate that most isolates may be CA-MRSA. Mastitis caused by MRSA isolates was significantly less resistant to SXT (0), fucidin (0), clindamycin (72.7%), and erythromycin (72.7%) than those of other MRSA clinical syndromes. SCCmec IV and V types accounted for 81.9% of mastitis-associated MRSA. PVL genes were frequently identified. Mastitis had progressed to breast abscess and required surgical drainage in 1 MRSA infection, carrying PVL gene. None of the MRSA strains isolated from patients with mastitis carried the TSST-1 genes.
The SmaI-digested PFGE analysis of the 16 MRSA isolates associated with NF identified 8 PFGE patterns, 11 MRSA isolates associated with mastitis identified 6 PFGE patterns. Pulsotypes A, C, and D accounted for 68.8% of NF isolates and 72.7% of mastitis isolates.
Conclusions: MRSA is a significant emerging cause of NF and mastitis in Taiwan. In endemic areas, treatment of NF and mastitis patients should target MRSA with initial empiric antibiotics, exclusive erythromycin and clindamycin.
廖明輝 教授兼系主任 (Ming-Huei Liao)
屏東科技大學獸醫系 生物科技研究所
最高學歷：國立臺灣大學碩士 期刊論文：
1.
Chiu SY, et al., The oldest bacteria of this earth in New Mexico of the states. A Environ Microbiol 10: 59-62, 2002.
2.
Chiu SY, et al., Studies of body temperatures: (I) body temperatures of various animals including human beings. A Environ Microbiol 10: 65-73, 2002.
3.
Chiu SY, et al., Studies of body temperatures: (II) body temperatures of Sprague-Dawley rats. A Environ Microbiol 10: 75-82, 2002.
4.
Chiu SY, et al., Microscopic crystal structures of chemicals: (II) some organic chemicals with complex structures of crystals. A Environ Microbiol 10: 83-89, 2002.
5.
Chiu SY, et al., Studies of eyes of cat(s): (III) ophthalmic fundus of Siamese cats in comparison with that of pursing cats. A Environ Microbiol 10: 91-97, 2002.
6.
Liu HJ, et al., Development of an ELISA for detection of antibodies to avian reovirus in chickens. J Virol Methods 102: 129-138, 2002. (SCI)
7.
Liao MH, et al., Microbial isolations from clinical specimens of a surgical hospital of this country during the crossing period of 20th and 21st century.? A Environ Microbiol 11: 1-8, 2002.
8.
Chiu SY, et al., Antibiotics having high percentage of sensitive strains among the clinical isolates. A Environ Microbiol 11: 9-14, 2002.
9.
Chiu SY, et al., Microscopic views: (IV) some biological items with structural details including feeler of snail, leg of fly-eater and floating aqua plant. A Environ Microbiol 11: 15-23, 2002.
10.
Chiu SY, et al., Mar PH. Antibiotics having more than 90% resistant isolates during 1998-2001. A Environ Microbiol 11: 27-33, 2002.
11.
Chiu SY
, et al., Eyelids responses and pupils reactions of Siamese cats and Pursang cats. A Environ Microbiol 11: 35-40, 2002.
12.
Chiu SY, et al., Microscopic vuews: (II) egg capsule of tiny blattella germanica and antennae of land snail. A Environ Microbiol 11: 41-48, 2002.
1.13.
Chiu SY, et al., Appearance of arthropod Taiwanese prior to the 331 earthquack in Taipei city. A Environ Microbiol 11: 51-57, 2003.
2.14.
Chiu SY, et al., Investigations of merchandize, electric instruments, house safety, refrigerators and electronic calculators during the 331 earthquack in Taipei city. A Environ Microbiol 11: 59-70, 2003.
3.15.
Chiu SY, Liao MH, Su YC, Mar PH. Pseudofood without nutritional value found in readily eatable package or canes, drinks or tap water of this isolated island of west pacific ocean. A Environ Microbiol 11: 71-81, 2003.
4.16.
Liu HJ, et al., Detection of infectious bronchitis virus by multiple polymerase chain reaction and nucleotide sequence analysis. J Virol Methods 109: 31-37, 2003. (SCI)
5.17.
Chiu SY, et al., Chronological curves of some legal infectious diseases including hemorrhagic fever, rabies and plague of this country in the 20th century. A Environ Microbiol 12: 1-8, 2003.
6.18.
Chiu SY, et al., Minimal pupil size and rotation of eye ball of Siamese cat. A Environ Microbiol 12: 15-19, 2003.
7.19.
Yin CC, et al., Identification of Ehrlichia canis in cats by nested polymerase chain reaction and nucleotide sequence analysis. Taiwan Vet J 29: 122-128, 2003.
8.20.
Chiu SY, et al., Comparative analysisof changing of pupil shape upon light shinning on siamese cat(s) and pursingcat(s). A Environ Microbiol 12: 23-29, 2003.
9.21.
Chiu SY, et al., Some plants grown in the garden with indoor decorational value without roots. A Environ Microbiol 12: 37-41, 2003.
10.22.
Liu HJ, et al., Molecular evolution of avian reovirus: evidence for genetic diversity and reassortment of the S-class genome segments and multiple cocirculating lineages. Virology 314: 336-349, 2003. (SCI)
11.23.
Chiu SY, et al., The external structures in some detail of a moth found in a high building. A Environ Microbiol 12: 51-55, 2003.
12.24.
Chiu SY, et al., Antibiotics with moderate percentages of sensitive isolates during 1998-2001. A Environ Microbiol 12: 57-64, 2003.
13.25.
Yu FL, et al., Induction of hepatoma cells migration by phosphoglucose isomerase/autocrine motility factor through the upregulation of matrix metalloproteinase-3. Biochem Biophy Res Comm 314: 76-82, 2004. (SCI)
1426.
Chang CJ, et al., Apoptosis induced by bovine ephemeral fever virus. J Virol Methods 122: 165-170, 2004. (SCI)
1527.
Yu FL, et al., Hepatitis B virus X protein promotes cell migration by inducing matrix metalloproteinase-3. J Hepatol 42: 520-527, 2005. (SCI)
O6 犬隻沙氏桿菌分子流行病學之調查
1劉紋紋 2陳玄峰 1盧明志 1廖明輝
1國立屏東科技大學獸醫