蛋白质一级结构分析
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ProtParam
User-provided sequence:
10 20 30 40 50 60 MLKKQEKFYY GGDYNPEQWD ESVWKEDMRL MKKAGVNYVS INIFSWARLQ PDEETYDFST
70 80 90 100 110 120 LDKIMDMLAE NGIGADLATA TAAPPAWLSR KYPDSLPVDK DGARFLPGSR QHYCPNSKDY
130 140 150 160 170 180 ARLAAKLVRK IAERYKNHPA LVMWHVNNEY GCHIAECYCD NCKKAFQTWL KEKYSTIENL
190 200 210 220 230 240 NKSWSTDFWS QRYYEWEEIC LPGKTPTFAN PMQQLDYKAF MDDSLLALYK MERDILKAYT
250 260 270 280 290 300 PDVPVMTNLM GLHKPVDGFH WAKEMDLVTW DAYPDPFDRI PYAQFMAHDL MRSLKKQPFL
310 320 330 340 350 360 LMEQAAGAVN WRAQNAVKTP GQMRLWSYEA AAHGADGIMF FQWRASQGGA EKFHSGMVPH
370 380 390 400 410 420 SGDEESRNFR EVVQLGNELK NLEKVTGSAY AADVAIVFDW KNWWALELDS KPSSLVTYIK
430 440 450 460 470 480 QLLPFYKVLH TRNIGVDFIH PDEAMDRYKV VFAPASYRVT KAFADKVKDY VEKGGYFVSN
490 500 510 520 530 540 FFSGIADEND RVYLGGYPGA YRDILGIYVE EFAPMKKGAV HQIRTGYGDA AIRVWEEKIH
550 560 570 580 590 600 LKGAEALAWF KDGYLAGSPA VTAHHCGKGK AYYIGTQPDE QYLSSLLKDI LQEADVRPAL
610 620 630 640 650 660 DAPRGVEVVV RKNGNEKYLF LLNHTDQVQF VNAGGTYLEL INGDTESETV RLSPRDVKIL
QVIEK
References and documentation are available.
Number of amino acids: 665
Molecular weight: 76089.5
Theoretical pI: 6.06
Amino acid composition:
Ala (A) 63 9.5%
Arg (R) 29 4.4%
Asn (N) 27 4.1%
Asp (D) 47 7.1%
Cys (C) 7 1.1%
Gln (Q) 24 3.6%
Glu (E) 42 6.3%
Gly (G) 43 6.5%
His (H) 17 2.6%
Ile (I) 26 3.9%
Leu (L) 54 8.1%
Lys (K) 51 7.7%
Met (M) 20 3.0%
Phe (F) 28 4.2%
Pro (P) 32 4.8%
Ser (S) 31 4.7%
Thr (T) 24 3.6%
Trp (W) 19 2.9%
Tyr (Y) 38 5.7%
Val (V) 43 6.5%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 89Total number of positively charged residues (Arg + Lys): 80
Atomic composition:
Carbon C 3451
Hydrogen H 5221
Nitrogen N 907
Oxygen O 988
Sulfur S 27
Formula: C
3451H
5221
N
907
O
988
S
27
Total number of atoms: 10594
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 161495
Abs 0.1% (=1 g/l) 2.122, assuming all pairs of Cys residues form cystines
Ext. coefficient 161120
Abs 0.1% (=1 g/l) 2.118, assuming all Cys residues are reduced Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.16
This classifies the protein as stable.
Aliphatic index: 75.14
Grand average of hydropathicity (GRAVY): -0.451
EXPASY ——protparam