Title page
XXX attenuate XXX‐induced XXX disease through/by the inhibition/regulation of
XXX
Running title: XXX attenuate XXX disease
Xiao‐ming Wang1, San Zhang2, Chong Ling‐hu1*
1 department of XXX, XXX university, XXX city 123456, China.
2 department of XXX, XXX university, XXX city 123456, China.
*Correspondence to:
Professor Chong Ling‐hu, M.D., Ph.D.
Department of Clinical Medicine，XXX University City College School of Medicine 000 XXX Street, XXX city 123456，P.R. China
Email: XXX@
Abstract
Background
Rational for study and gap in knowledge (1‐2 sentences)
Hypothesis or purpose statement (1 sentence)
General experimental design (optional)
Methods (One result with one method.)
XXX was determined/detected/measured/evaluated/examined by XXX method/experiment.
We determined/detected/measured/evaluated/examined the XXX level using XXX method/experiment.
Ect.
Results
We fonnd that ….
Compared with …. group, XXX was increased/decreased/enhanced/reduced by XXX treatment/administrations.
Conclusions and implications
These results/data were suggested/indicated/demonstrated that …..
Key word: 5‐6 key words.
Introduction
Background：Known (General to specific)
Unknown: gap, how study will fill gap, and why filling gap is important
Until now, it has not been possible to identify ….
However, we do not know what effect this treatment will have on ….
There has been some disagreement, however, in the findings with this model….
So far, only qualitative findings have been obtained…
Because of the rarity of this cancer, it has been difficult to determine survival rates with statistical certainty…
Hypothesis
The purpose of our study was to determine ….
We hypothesized that ….
Our hypothesis was that….
Strategy
We tested our hypothesis by examining….
To do this, we used … and analyzed … in an situ model ….
To evaluate/investigate/explore the inhibitory/regulatory effect of … on …., the …. Assay/methods/experiments were employed/used.
Conclusion
Overall, our findings that … confirm/indicate/suggest/demonstrate ….
In this study, we found/observed/demonstrated that ….
In the present study, we found/observed/demonstrated that ….
Materials and methods
Materials and reagents
Drugs, reagents, equipment, antibodies, ect.
Cell lines and cultures
Which ones cells were used in this study?
What is the suppliers of the cells?
How the cells were prepared?
How the cells were cultured?
How the cells were treated?
How the cells were grouped?
How the cell‐samples were collected?
Human subjects
IRB approval;
Infomed consent;
Source of study population;
Inclusion and exclusion criteria;
Sample collection and number of samples.
