HPLC 法分析不同产地生何首乌中的蒽醌类化合物
潘文嘉;林双;黎文敏;林坚涛
【期刊名称】《医药前沿》
【年(卷),期】2012(000)036
【摘要】Objective: To determine the contents of anthraquinone before and after hydrolysis in the commercial medicinal materials of Radix Polygomi Multiflori colected from different areas respectively by RP-HPLC. Methods: The determination was carried out using C18 column,with mobile phase consisted of methanol-0.1% phosphoric acid solution(85:15),the detection wavelength was 254 nm. Results: Anthraquinone contents in Sichuan Emei Mountain sample is maximal.The free type and union type emodin contents were 235.59mg/100g, 18.17mg/100g and the free type physcion was 97.69 mg/100g .The contents in the Chongqing Jin Yun county , Guizhou Shi Bing county, Guangxi Tian Yang sample were lower. Conclusion: Anthraquinone contained in the samples were mainly emodin and physcion.The contents varied greatly in the samples from the different producing areas. Make a Radix Polygomi Multiflori quality evaluation has provided the reliable index and method.% 目的：采用反相高效液相色谱法测定十个已知产地的生首乌水解前、后蒽醌类成分的含量,从而评价不同采集地生何首乌的质量.方法：HPLC 法,以C18为色谱柱,甲醇-0.1%磷酸(85：15)为流动相,流速为1.0ml?min -1；检测波长254nm；结果：四川峨眉山样