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第十二单元第38讲随堂反馈高效突破

第十二单元第38讲随堂反馈高效突破
第十二单元第38讲随堂反馈高效突破

1.如图甲、乙是微生物接种常用的两种方法,请回答相关问题:

(1)图甲是利用________________法进行微生物接种,图乙是利用________________法进行微生物接种。这两种方法所用的接种工具分别是________和________;对这两种接种工具进行灭菌和消毒的方法依次是________和酒精消毒。

(2)接种操作为什么一定要在火焰附近进行?____________________________________

__________________________________________________________。

(3)接种后,在固体培养基上培养细菌时为什么进行倒置培养?________________________________________________________________________。

(4)如图是采用上述接种方法接种后培养的效果图解,其接种方法是________(填图甲或图乙)。

2.(2014·北京东城联考)高温淀粉酶在大规模工业生产中有很大的实用性。研究者从热泉中筛选了高效产生高温淀粉酶的嗜热菌,其筛选过程如图所示。

(1)进行①过程的目的是________;②过程所使用的接种方法是________________法。

(2)从用途上来说,Ⅰ号培养基属于________培养基,仅以淀粉作为________源;从物理状态上来说,Ⅱ号培养基属于固体培养基,配制时应加入________作为凝固剂。

(3)Ⅰ、Ⅱ号培养基配制和灭菌时,灭菌与调节pH的先后顺序是________________;一般对配制的培养基采用________________法灭菌。

(4)部分嗜热菌在Ⅰ号培养基上生长时可释放________分解培养基中的淀粉,在菌落周围形成透明圈;应挑出透明圈________(大或小)的菌落,接种到Ⅱ号培养基。

3.(2013·高考四川卷改编) 普通酵母菌直接利用淀粉的能力很弱,有人将地衣芽孢杆菌的α-淀粉酶基因转入酵母菌中,经筛选得到了可高效利用淀粉的工程酵母菌菌种(过程如图甲所示)。

(1)图甲中,过程①需要的酶有____________________。为达到筛选目的,平板内的固

体培养基应以________作为唯一碳源。②、③过程需重复几次,目的是________________________________________________________________________ ________________________________________________________________________。

(2)某同学尝试过程③的操作,其中一个平板经培养后的菌落分布如图乙所示。该同学的接种方法是______________;推测该同学接种时可能的操作失误是______________。

(3)以淀粉为原料,用工程酵母菌和普通酵母菌在相同的适宜条件下密闭发酵,接种________________菌的发酵罐需要先排气,其原因是________________________________________________________________________

________________________________________________________________________。

1.解析:图甲和图乙分别是平板划线法和稀释涂布平板法,这两种方法所用的接种工具分别是接种环和涂布器。由于在酒精灯火焰附近存在着无菌区域,因此接种常在火焰附近进行。在微生物培养过程中会产生水,对微生物的生长造成影响,故需要倒置培养。

答案:(1)平板划线稀释涂布平板接种环涂布器灼烧灭菌(2)火焰附近存在着无菌区域(3)避免培养过程产生的水分影响微生物的生长(4)图乙

2.解析:(1)由图中显示,在①号试管中细菌数目减少,可推知此过程为稀释;Ⅰ号培养基中微生物菌落均匀分布,可确定②使用的接种方法是稀释涂布平板法。(2)由图示可知②过程是分离能产生高温淀粉酶的嗜热菌的过程,Ⅰ号培养基应为以淀粉作为唯一碳源的选择培养基,培养基呈固体状是因为配制时加入了琼脂作为凝固剂。(3)制备培养基时应先调节pH、后灭菌,对培养基进行灭菌的常用方法是高压蒸汽灭菌法。(4)部分嗜热菌之所以能分解淀粉,是因为此类细菌在生长时释放了淀粉酶,培养基中的淀粉被分解后,会形成大小不一的透明圈,其中透明圈越大,说明该菌落是高效产生高温淀粉酶的嗜热菌。

答案:(1)稀释稀释涂布平板(2)选择(唯一)碳琼脂(3)先调节pH,后灭菌高压蒸汽灭菌(4)淀粉酶大

3.解析:(1)由图甲基因工程操作的基本过程可以看出,①为基因工程中基因表达载体的构建过程,需要用到的工具酶有限制性核酸内切酶和DNA连接酶。为筛选转基因酵母菌,需对酵母菌进行选择培养,即将酵母菌培养在以淀粉为唯一碳源的培养基中。为提高转基因酵母菌的纯度,需要多次筛选。(2)分离纯化某种菌的常用方法有稀释涂布平板法和平板划线法。由菌落分布情况可以看出,该同学采用的是稀释涂布平板法,且由于涂布不均匀造成了乙图所示现象。(3)由于工程酵母菌利用淀粉的能力强,发酵产生酒精和CO2的速率较快,故需要先排气。

答案:(1)限制性核酸内切酶、DNA连接酶淀粉进一步筛选纯化获得分解淀粉能力强的酵母菌(2)稀释涂布平板法涂布不均匀

(3)工程酵母工程酵母菌分解淀粉产生葡萄糖的能力强,导致酒精发酵产生CO2的速率更快

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一、选择填空 1.Bill’s sister has _____ MP4. A. an B. the C. / D. a 2.What does your sister like _____ _breakfast ? A. with B. in C. for D. at 3.What’s your brother’s name ? ____name is Victor Green. A. He B. She C.Her D.His 4.What are _____ under the chair ? They are books . A.the B. this C. those D. that 5.Can you ____your tennis racket to me ? A. bring B. call C. take D. like 6.His uncle plays tennis very _______ A.good B. fine C. nice D. well 7.Linda ____7basketball games every evening. A. looks B. look C. watch D. watches 8.Mom, ____my friend, Jane. A. she is B. here is C. he is D. this is 9.Mrs Green has three daughters, ___she loves them very much. A. but B. for C. and D. with 10. I can’t play ping-pong.It is _ A. difficult B. fun C. interesting D. relaxing 11.I like carrots, but Mona doesn’t like _____ A. it B.them C. this D. these 12.Does Peter have a tennis racket ? ___________. But he has some tennis. A.Yes, he does B.Yes, he is C.No, he isn’t D.No, he doesn’t 13. How is your grandmother,Tom? ________. A. She is 62 years old B. She is OK C. I'm fine D. sorry 14.It’s October 10th. Lucy is _______ years old now. Today is her ______ birthday. A. twelve twelvth B. twelve twelve C. twelfth twelve D. twelve twelfth 15. He usually watches TV S aturday evening. A.on B.in C.at D.for 16. ___your brother___ a baseball? A. Do,has B. Does, have C. Does has D. Don't have 17. --_____is my computer game? ---It’s in the drawer.

《学英语随堂反馈》提高卷 Unit 7 of 7B

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家长开放日反馈表05403

玛纳斯县电厂学校“家长开放日”活动反馈表 贵家长:您好! 真诚地感谢您百忙之中抽出时间来参加这次“家长开放日活动”!您的参与是对我们学校工作的支持,您的意见和建议对我校的发展尤为重要。请您结合本次“家长开放日”活动如实填写这张反馈表,感谢您的参与和支持! (请在您赞同的观点的()里打“√”) 1、开放日活动所听的课中,您认为上得最好的课是 语文()数学()英语()体育()音乐()其他() 2、听完课后,您对老师的表现 满意()较满意()一般()不满意() 3、听完课后,您对您孩子今天课上的表现 满意()较满意()一般()不满意() 4、您对教师关爱学生、教育学生的态度 满意()较满意()一般()不满意() 5、您对教师教育教学的能力和水平 满意()较满意()一般()不满意() 6、您对此次“家长开放日”活动的满意度: 满意()较满意()一般()不满意() 7、您认为有必要进行家长开放日活动吗?为什么? 8、您对学校管理的总体印象 非常满意()满意()基本满意()不满意() 9、您想对班主任或任课教师说的是: 10、您对学校、班级目前的教育、教学及管理工作有何意见或建议:

谢谢您对本次活动的支持与配合!我们将根据您所提出的宝贵意见,认真反思,总结经验,改进不足,不断促进学校发展。 玛纳斯县电厂学校 玛电学校家长开放日听课记录表 温馨提示: 为了让孩子们的课堂活动正常、有序地进行,届时请您配合做好以下事项: 1、听课时,请您准时参加,认真听课,不要交谈,将手机置于调为震动或者关机状态,不接打电话。 2、听课后,我们准备了一份《家长听课记录表》和《反馈意见表》请您填写宝贵意见(请自备笔填写); 3、当天家长可以带目的地观看孩子在校的学习表现,当然这可能并不是最真实的,因为有您的存在,多少会给孩子带来影响。当我们看到孩子有异常表现时,请您不用惊讶和担心;当发现孩子有进步时,请给予他(她)及时的鼓

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随堂听课评课记录

随堂听课评课记录 听课、评课是教学研究的有效手段。在倡导大力开展校本教学研究的今天,大力开展听课评课活动、研究并改进教学中存在的问题尤其显得重要。以下是随堂听课评课记录,欢迎阅读。 一、复习导入。 1、师:小朋友,想去梅花山看梅花吗?先来读一读该课的词语,读对了词语,咱们就去看梅花。 2、(课件出示生字词) “开火车”读生词,齐读生词。 二、精读课文。 1、过渡:现在,就让咱们跟随小作者去看梅花吧。 2、学习第一自然段。 ⑴出示课文的第一自然段。(出示课件) 红笔标出:“盛开的梅花,你挨着我,我挨着你,像蓝天下的片片彩云,那一朵一朵的梅花,晶莹透亮,散发出淡淡的清香。” 指名读句子。 自由读。(用自己喜欢的方式读,边读边想) 【评:在教学过程中教予学生读书的方法,引导学生变机械的阅读为思考性的阅读】 ⑵小朋友,你们读了这句话想到了什么?说说看。 指名说。师生交流:(出示课件) ①盛开的梅花,你挨着我,我挨着你,像蓝天下的片片彩云。

“盛开”完全开放,“你挨着我,我挨着你”是说花非常之多之密,“像蓝天下的片片彩云”是说花儿色彩绚丽,开遍山坡。 ②“那一朵一朵的梅花,晶莹透亮,“散发出淡淡的清香”。 “晶莹透亮”写出梅花的光亮可爱,“散发出淡淡的清香”写出梅花的香味雅而不俗,沁人心脾。联想到那首王安石的《梅花》:遥知不是雪,为暗香有来。“暗香”就是指这淡淡的清香。 教师根据学生的回答,用红笔标出“片片”、“清香”、“淡淡”、“彩云”、“晶莹特亮”、“挨着”。 【评:充分发挥学生在课堂上的主体性。学生生动化的语言,教师机智地引导,让学生从文本对话中再现生动形象的梅花山画面,“一千个读者就有一千个哈姆雷特”,每个学生在自己的眼中构建了一个美丽的梅花山,更好地辅助学生理解课文,读好课文。】 ⑶指导朗读 ①师:小朋友们想象中的梅花上可真是美不胜收阿!让我们一起去看看吧! (播放配音录像) 【评:先在文本对话中引发学生的思考,进行自我艺术世界的构建,再给学生以视觉的冲击,完善艺术世界的构建,使学生更好地理解课文。】 ②“盛开的梅花,你挨着我,我挨着你,像蓝天下的片片彩云”。(出示课件) 梅花多吗?美吗?

牛津译林版英语九上《Unit3》随堂反馈提高卷

学英语随堂反馈提高卷Test for Unit 3 of 9A Class ____________ Name ____________ (整卷满分为90分) 一、单项填空(15分) ( ) 1. —Look! She’s running so fast! —Hard to _______ her legs were once broken. A. know B. imagine C. realize D. find ( ) 2. I don’t doubt _______ she can do it well, but I doubt _______ she is willing to do it. A. that; that B. if; if C. that; if D. if; that ( ) 3. Half of the class _______ done most of the work. The left _______ rather difficult. A. have, is B. has, is C. have, are D. has, are ( ) 4. There is nothing _______. A. for us to worry B. of us to worry C. for us to be worried D. for us to worry about ( ) 5. ––_______ we go camping this summer holiday? ––Nothing _______ be better! A. Will; need to B. Shall; could C. Could; must D. Shall; have to ( ) 6. Could you tell me _______? A. who can help us organize the show B. how long you have bought the car C. what was your favourite subject D. how many students are there in your class ( ) 7. —Nobody but you _______ late for school this morning. —Sorry , I _______ to school when it began to rain heavily. A. was; went B. were; was going C. was; was about to go D. is; am going ( ) 8. —_______ I take my umbrella with me? —_______ better to do so. The weather is changeable. A. Must; You’d B. Shall; It’s C. Need; You’d D. Would; It’s ( ) 9. Little Tom seems to know who _______ to get more pocket money. A. can he talk to B. he can talk C. to talk to D. to talk ( ) 10. Why not _______ your teacher for help when you can’t finish _______ the story by yourself? A. to ask; write B. to ask; writing C. ask; writing D. asking; to write ( ) 11. Being _______, the novel written by a famous teacher sells _______. A. very worth of being read; good B. well worth reading; good C. well worth reading; well D. very worth reading; well ( ) 12. Let me _______ if you have any problems _______ the problems. I’ll try my best to help you. A. to know; to work out B. know; working out C. know; worked out D. knew; working on ( ) 13. Fred loved Maths, but believe it or not, he _______ passed the last exam. A. only B. easily C. almost D. hardly ( ) 14. ––Excuse me, could you tell me _______?––Yes. It’s about twenty minutes’ walk. A. how long it takes to go to the park B. how far it is from here to the park C. how does it take to go to the park D. how far is it from here to the park ( ) 15. ––You must be very excited about going to New York for further study. ––, but I am afraid I won’t do well because my English is poor. A. I’m sorry B. Certainly not C. I don’t know yet D. Well, I should be 二、完形填空(10分) I had said goodbye to my husband, Joe, so often, but this time was 1 _ . We now had our first child. After nights of talks, we made the difficult decision that Joe would go by himself to Alabama for the six-month training course, and I would stay behind with our new son. It was important that I 2 _ my teaching position near our home. Also some friends could come for help. On Joe’s 3 _ evening at home, I bathed little Joey and was heading the bedroom when Joe gently

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